Restriction map for
pFN21AB4783
(Circular) (Six-base) MAPSORT of: pFN21AB4783.seq Check: 5157 from: 1 to: 6661
pFN21AB4783 6661 bp
With 182 enzymes: SgfI *
February 27, 2009 14:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2542 2542
Size: 6661
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2680 4471 278
Size: 53 83 186 936 1144 1791 2468
Fragments arranged by size:
2468 1791 1144 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4004 1937
Size: 2067 4594
Acc65I G'GTAC_C
Cuts at: 3314 3982 3314
Size: 668 5993
AclI AA'CG_TT
Cuts at: 1563 4788 5161 1563
Size: 3225 373 3063
Fragments arranged by size:
3225 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3471 4724 6108 6376 1632
Size: 1839 1253 1384 268 1917
Fragments arranged by size:
1917 1839 1384 1253 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6464
AflIII A'CryG_T
Cuts at: 1203 5560 6537 1203
Size: 4357 977 1327
Fragments arranged by size:
4357 1327 977
AgeI A'CCGG_T
Cuts at: 5492 5492
Size: 6661
AhdI GACnn_n'nnGTC
Cuts at: 5390 5390
Size: 6661
AleI CACnn'nnGTG
Cuts at: 1166 2827 1166
Size: 1661 5000
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6661
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2766 2798 2766
Size: 32 6629
AlwNI CAG_nnn'CTG
Cuts at: 2824 3847 4404 5976 2824
Size: 1023 557 1572 3509
Fragments arranged by size:
3509 1572 1023 557
ApaLI G'TGCA_C
Cuts at: 3275 4718 5874 3275
Size: 1443 1156 4062
Fragments arranged by size:
4062 1443 1156
ApoI r'AATT_y
Cuts at: 1493 2093 2164 3640 3970 4196 4348 6457 1493
Size: 600 71 1476 330 226 152 2109 1697
Fragments arranged by size:
2109 1697 1476 600 330 226 152 71
AseI AT'TA_AT
Cuts at: 160 1032 5215 160
Size: 872 4183 1606
Fragments arranged by size:
4183 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6661
AvaI C'yCGr_G
Cuts at: 1870 1943 3986 1870
Size: 73 2043 4545
Fragments arranged by size:
4545 2043 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3298 3331 3298
Size: 33 6628
BamHI G'GATC_C
Cuts at: 3991 4358 3991
Size: 367 6294
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3172 3314 3686 3710 3982
Size: 325 217 627 1385 142 372 24 272
Cuts at: 3982 5437 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 1385 627 372 325 272 217
142 24
BanII G_rGCy'C
Cuts at: 1391 2148 3797 3980 1391
Size: 757 1649 183 4072
Fragments arranged by size:
4072 1649 757 183
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2435 928
Size: 971 536 5154
Fragments arranged by size:
5154 971 536
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4852 4886 1903
Size: 34 50 34 2831 34 3678
Fragments arranged by size:
3678 2831 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3119 4553 5763 3119
Size: 1434 1210 4017
Fragments arranged by size:
4017 1434 1210
BclI T'GATC_A
Cuts at: 1548 3237 1548
Size: 1689 4972
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2815 3392 5272
Size: 107 122 71 631 605 1143 577 1880
Cuts at: 5272 136
Size: 1525
Fragments arranged by size:
1880 1525 1143 631 605 577 122 107
71
BglII A'GATC_T
Cuts at: 2655 6656 2655
Size: 4001 2660
BlpI GC'TnA_GC
Cuts at: 4078 4078
Size: 6661
Bme1580I G_kGCm'C
Cuts at: 1790 3279 4722 5878 1790
Size: 1489 1443 1156 2573
Fragments arranged by size:
2573 1489 1443 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6661
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3786 4392 5350
Size: 416 53 83 399 24 2369 606 958
Cuts at: 5350 442
Size: 1753
Fragments arranged by size:
2369 1753 958 606 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6661
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2714 2924 2933 5321 1239
Size: 1475 210 9 2388 2579
Fragments arranged by size:
2579 2388 1475 210 9
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3714 4786 5651 5949 3714
Size: 1072 865 298 4426
Fragments arranged by size:
4426 1072 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5324 882
Size: 617 3825 2219
Fragments arranged by size:
3825 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3956 4452 493
Size: 711 2752 496 2702
Fragments arranged by size:
2752 2702 711 496
BsaBI GATnn'nnATC
Cuts at: 6655 6655
Size: 6661
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2677 4468 4850 5485
Size: 53 83 186 936 1144 1791 382 635
Cuts at: 5485 275
Size: 1451
Fragments arranged by size:
1791 1451 1144 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2182 3474 5094 5492 5766 5913 6324 2182
Size: 1292 1620 398 274 147 411 2519
Fragments arranged by size:
2519 1620 1292 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6436
Fragments arranged by size:
6436 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2028 3447 3555 3918 3921 3924 3933 3936
Size: 842 1419 108 363 3 3 9 3
Cuts at: 3936 1186
Size: 3911
Fragments arranged by size:
3911 1419 842 363 108 9 3 3
3
BseYI C'CCAG_C
Cuts at: 2538 5864 6360 2538
Size: 3326 496 2839
Fragments arranged by size:
3326 2839 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6088
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4111 4872 5900 1540
Size: 275 186 2110 761 1028 2301
Fragments arranged by size:
2301 2110 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2148 2614 3279 3658 3797 4722 4807 5878 2148
Size: 466 665 379 139 925 85 1071 2931
Fragments arranged by size:
2931 1071 925 665 466 379 139 85
BsmI GAATG_Cn'
Cuts at: 2760 4172 4265 2760
Size: 1412 93 5156
Fragments arranged by size:
5156 1412 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3046 3046
Size: 6661
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2148 2614 3279 3658 3797 3980 4722
Size: 399 358 466 665 379 139 183 742
Cuts at: 4722 4807 5878 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 665 466 399 379 358
183 139 85
BspEI T'CCGG_A
Cuts at: 2182 6324 2182
Size: 4142 2519
BspHI T'CATG_A
Cuts at: 4550 6280 4550
Size: 1730 4931
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2542 4016 844
Size: 1698 1474 3489
Fragments arranged by size:
3489 1698 1474
BsrBI CCG'CTC
Cuts at: 1394 1823 4548 1394
Size: 429 2725 3507
Fragments arranged by size:
3507 2725 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2406 5156 5330 65
Size: 1183 1158 2750 174 1396
Fragments arranged by size:
2750 1396 1183 1158 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3822 5305 5492 1700
Size: 110 2012 1483 187 2869
Fragments arranged by size:
2869 2012 1483 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6661
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6661
BssSI C'ACGA_G
Cuts at: 4721 5733 4721
Size: 1012 5649
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6661
BstBI TT'CG_AA
Cuts at: 5465 5465
Size: 6661
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6661
BstYI r'GATC_y
Cuts at: 1972 2655 2781 3991 4358 4743 4760 6212 6298
Size: 683 126 1210 367 385 17 1452 86
Cuts at: 6298 6310 6656 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1210 683 385 367 346 126
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2047 3112 3205 513
Size: 552 982 1065 93 3969
Fragments arranged by size:
3969 1065 982 552 93
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5922
Fragments arranged by size:
5922 683 56
BtsI GCAGTG_nn'
Cuts at: 3628 4177 4971 4991 3628
Size: 549 794 20 5298
Fragments arranged by size:
5298 794 549 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6661
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3535 3570 529
Size: 35 2971 35 3620
Fragments arranged by size:
3620 2971 35 35
DraI TTT'AAA
Cuts at: 3965 3965
Size: 6661
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5668 809
Size: 507 4352 1802
Fragments arranged by size:
4352 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2389 2556 2843
Size: 54 1425 50 275 18 559 167 287
Cuts at: 2843 3095 3286 3556 4108 4997 6626 8
Size: 252 191 270 552 889 1629 43
Fragments arranged by size:
1629 1425 889 559 552 287 275 270
252 191 167 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4108 1537
Size: 275 2296 4090
Fragments arranged by size:
4090 2296 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2582 3375 4591 1353
Size: 1229 793 1216 3423
Fragments arranged by size:
3423 1229 1216 793
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3456 5248 5622 5768 387
Size: 1064 138 1867 1792 374 146 1280
Fragments arranged by size:
1867 1792 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2146 3795 2146
Size: 1649 5012
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2714 2924 2933 3471 4724 5321 6108
Size: 393 1082 210 9 538 1253 597 787
Cuts at: 6108 6376 1239
Size: 268 1524
Fragments arranged by size:
1524 1253 1082 787 597 538 393 268
210 9
EcoNI CCTnn'n_nnAGG
Cuts at: 3350 3350
Size: 6661
EcoO109I rG'GnC_Cy
Cuts at: 2621 2621
Size: 6661
EcoRI G'AATT_C
Cuts at: 3970 4348 3970
Size: 378 6283
FspI TGC'GCA
Cuts at: 3083 5167 3083
Size: 2084 4577
HaeII r_GCGC'y
Cuts at: 1678 5808 1678
Size: 4130 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3185 3217 3351 3383 5302
Size: 32 135 32 1613 32 134 32 1919
Cuts at: 5302 5334 5376 5408 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1919 1613 135 134 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2797 3128 4005 4251 669
Size: 988 281 859 331 877 246 3079
Fragments arranged by size:
3079 988 877 859 331 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6661
HpaI GTT'AAC
Cuts at: 4251 4251
Size: 6661
KpnI G_GTAC'C
Cuts at: 3318 3986 3318
Size: 668 5993
MfeI C'AATT_G
Cuts at: 2454 4260 2454
Size: 1806 4855
MluI A'CGCG_T
Cuts at: 6537 6537
Size: 6661
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3013 5775 5959 1305
Size: 1708 2762 184 2007
Fragments arranged by size:
2762 2007 1708 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2845 3097 10
Size: 54 1425 343 1013 252 3574
Fragments arranged by size:
3574 1425 1013 343 252 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2827 4619 4978 5137 518
Size: 648 1661 1792 359 159 2042
Fragments arranged by size:
2042 1792 1661 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3701 4077 4754 5902 6147 1606
Size: 2095 376 677 1148 245 2120
Fragments arranged by size:
2120 2095 1148 677 376 245
NcoI C'CATG_G
Cuts at: 513 1065 3112 513
Size: 552 2047 4062
Fragments arranged by size:
4062 2047 552
NdeI CA'TA_TG
Cuts at: 387 2206 387
Size: 1819 4842
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6661
NotI GC'GGCC_GC
Cuts at: 4108 4108
Size: 6661
NspI r_CATG'y
Cuts at: 1132 1229 4019 5564 1132
Size: 97 2790 1545 2229
Fragments arranged by size:
2790 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 3338 1348
Size: 1990 4671
PciI A'CATG_T
Cuts at: 5560 5560
Size: 6661
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2947 2995 1108
Size: 1839 48 4774
Fragments arranged by size:
4774 1839 48
PfoI T'CCnGG_A
Cuts at: 2671 2691 3874 2671
Size: 20 1183 5458
Fragments arranged by size:
5458 1183 20
PmeI GTTT'AAAC
Cuts at: 3965 3965
Size: 6661
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4730 4762 6243 6275 4730
Size: 32 1481 32 5116
Fragments arranged by size:
5116 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2621 2621
Size: 6661
PshAI GACnn'nnGTC
Cuts at: 1613 3123 1613
Size: 1510 5151
PsiI TTA'TAA
Cuts at: 4231 4231
Size: 6661
PstI C_TGCA'G
Cuts at: 830 4013 830
Size: 3183 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6661
PvuII CAG'CTG
Cuts at: 3701 3701
Size: 6661
SacI G_AGCT'C
Cuts at: 2148 3797 2148
Size: 1649 5012
SalI G'TCGA_C
Cuts at: 1936 4003 1936
Size: 2067 4594
SbfI CC_TGCA'GG
Cuts at: 4013 4013
Size: 6661
ScaI AGT'ACT
Cuts at: 2593 4909 6621 2593
Size: 2316 1712 2633
Fragments arranged by size:
2633 2316 1712
SfcI C'TryA_G
Cuts at: 826 1045 3189 4009 5144 5825 6016 826
Size: 219 2144 820 1135 681 191 1471
Fragments arranged by size:
2144 1471 1135 820 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6661
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6661
SmaI CCC'GGG
Cuts at: 1872 3988 1872
Size: 2116 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3729 4765 5666 5928 820
Size: 197 926 1786 1036 901 262 1553
Fragments arranged by size:
1786 1553 1036 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4452 493
Size: 3959 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6661
SphI G_CATG'C
Cuts at: 1132 4019 1132
Size: 2887 3774
SspI AAT'ATT
Cuts at: 5 52 3036 4585 5
Size: 47 2984 1549 2081
Fragments arranged by size:
2984 2081 1549 47
StuI AGG'CCT
Cuts at: 1517 2809 1517
Size: 1292 5369
StyI C'CwwG_G
Cuts at: 513 1065 3112 4100 513
Size: 552 2047 988 3074
Fragments arranged by size:
3074 2047 988 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2906 3190 3543 4688 4705 4858 5043
Size: 1255 1326 284 353 1145 17 153 185
Cuts at: 5043 5488 325
Size: 445 1498
Fragments arranged by size:
1498 1326 1255 1145 445 353 284 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2545 2591 2723
Size: 274 80 33 51 1443 568 46 132
Cuts at: 2723 4907 6619 96
Size: 2184 1712 138
Fragments arranged by size:
2184 1712 1443 568 274 138 132 80
51 46 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6621 6653 6621
Size: 32 6629
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6661
XbaI T'CTAG_A
Cuts at: 2579 3997 2579
Size: 1418 5243
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3138 3674 3809 3138
Size: 536 135 5990
Fragments arranged by size:
5990 536 135
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6661
XmaI C'CCGG_G
Cuts at: 1870 3986 1870
Size: 2116 4545
XmnI GAAnn'nnTTC
Cuts at: 4790 4790
Size: 6661
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2678 4469 276
Size: 53 83 186 936 1144 1791 2468
Fragments arranged by size:
2468 1791 1144 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI PvuII SacI SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BbeI BbvCI BfrBI BplI
Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PmlI PspOMI PspXI PsrI RsrII SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI