Restriction map for
pFN21AB4782
(Circular) (Six-base) MAPSORT of: pFN21AB4782.seq Check: 5641 from: 1 to: 5746
pFN21AB4782 5746 bp
With 182 enzymes: SgfI *
February 27, 2009 14:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2303 2303
Size: 5746
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3556 278
Size: 53 83 186 936 2020 2468
Fragments arranged by size:
2468 2020 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3089 1937
Size: 1152 4594
Acc65I G'GTAC_C
Cuts at: 3067 3067
Size: 5746
AclI AA'CG_TT
Cuts at: 1563 3873 4246 1563
Size: 2310 373 3063
Fragments arranged by size:
3063 2310 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2345 2680 3809 5193 5461 1632
Size: 713 335 1129 1384 268 1917
Fragments arranged by size:
1917 1384 1129 713 335 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5549
AflIII A'CryG_T
Cuts at: 1203 2637 2745 4645 5622 1203
Size: 1434 108 1900 977 1327
Fragments arranged by size:
1900 1434 1327 977 108
AgeI A'CCGG_T
Cuts at: 4577 4577
Size: 5746
AhdI GACnn_n'nnGTC
Cuts at: 2797 4475 2797
Size: 1678 4068
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5746
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5746
AlwNI CAG_nnn'CTG
Cuts at: 2062 3489 5061 2062
Size: 1427 1572 2747
Fragments arranged by size:
2747 1572 1427
ApaLI G'TGCA_C
Cuts at: 3002 3803 4959 3002
Size: 801 1156 3789
Fragments arranged by size:
3789 1156 801
ApoI r'AATT_y
Cuts at: 1493 3055 3281 3433 5542 1493
Size: 1562 226 152 2109 1697
Fragments arranged by size:
2109 1697 1562 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4300 160
Size: 872 3268 1606
Fragments arranged by size:
3268 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5746
AvaI C'yCGr_G
Cuts at: 1870 1943 2073 2915 3071 1870
Size: 73 130 842 156 4545
Fragments arranged by size:
4545 842 156 130 73
BamHI G'GATC_C
Cuts at: 2760 3076 3443 2760
Size: 316 367 5063
Fragments arranged by size:
5063 367 316
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3067 4522 618
Size: 325 217 627 1280 1455 1842
Fragments arranged by size:
1842 1455 1280 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2843 3065 1391
Size: 1452 222 4072
Fragments arranged by size:
4072 1452 222
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2318 2454 928
Size: 971 419 136 4220
Fragments arranged by size:
4220 971 419 136
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3937 3971 1903
Size: 34 50 34 1916 34 3678
Fragments arranged by size:
3678 1916 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2990 3638 4848 2990
Size: 648 1210 3888
Fragments arranged by size:
3888 1210 648
BclI T'GATC_A
Cuts at: 1548 2505 1548
Size: 957 4789
BfrBI ATG'CAT
Cuts at: 2596 2596
Size: 5746
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4357 136
Size: 107 122 71 631 605 2685 1525
Fragments arranged by size:
2685 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2337 5741 2337
Size: 3404 2342
BlpI GC'TnA_GC
Cuts at: 3163 3163
Size: 5746
Bme1580I G_kGCm'C
Cuts at: 1790 2086 3006 3807 4963 1790
Size: 296 920 801 1156 2573
Fragments arranged by size:
2573 1156 920 801 296
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5746
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3477 4435 442
Size: 416 53 83 399 24 2060 958 1753
Fragments arranged by size:
2060 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5746
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2944 2944
Size: 5746
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2492 2819 4406 1239
Size: 1253 327 1587 2579
Fragments arranged by size:
2579 1587 1253 327
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3871 4736 5034 3871
Size: 865 298 4583
Fragments arranged by size:
4583 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2699 2977 4409 882
Size: 617 1200 278 1432 2219
Fragments arranged by size:
2219 1432 1200 617 278
BsaAI yAC'GTr
Cuts at: 493 1204 3001 3537 493
Size: 711 1797 536 2702
Fragments arranged by size:
2702 1797 711 536
BsaBI GATnn'nnATC
Cuts at: 3026 5740 3026
Size: 2714 3032
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2355 2658 2735 3553
Size: 53 83 186 936 822 303 77 818
Cuts at: 3553 3935 4570 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 822 818 635 382 303 186
83 77 53
BsaWI w'CCGG_w
Cuts at: 2752 4179 4577 4851 4998 5409 2752
Size: 1427 398 274 147 411 3089
Fragments arranged by size:
3089 1427 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5521
Fragments arranged by size:
5521 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2066 1186
Size: 880 4866
BseYI C'CCAG_C
Cuts at: 2025 4949 5445 2025
Size: 2924 496 2326
Fragments arranged by size:
2924 2326 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2354 2588 2596 1163
Size: 573 618 234 8 4313
Fragments arranged by size:
4313 618 573 234 8
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3196 3957 4985 1540
Size: 275 186 1195 761 1028 2301
Fragments arranged by size:
2301 1195 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2592 2925 3006 3807 3892 4963 2592
Size: 333 81 801 85 1071 3375
Fragments arranged by size:
3375 1071 801 333 85 81
BsmI GAATG_Cn'
Cuts at: 2596 3257 3350 2596
Size: 661 93 4992
Fragments arranged by size:
4992 661 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2086 2592 2843 2925 3006 3065 3807
Size: 399 296 506 251 82 81 59 742
Cuts at: 3807 3892 4963 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 506 399 296 251 85
82 81 59
BspEI T'CCGG_A
Cuts at: 5409 5409
Size: 5746
BspHI T'CATG_A
Cuts at: 3635 5365 3635
Size: 1730 4016
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2303 3101 844
Size: 1459 798 3489
Fragments arranged by size:
3489 1459 798
BsrBI CCG'CTC
Cuts at: 1394 1823 3633 1394
Size: 429 1810 3507
Fragments arranged by size:
3507 1810 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4241 4415 65
Size: 1183 2993 174 1396
Fragments arranged by size:
2993 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4390 4577 1700
Size: 110 2580 187 2869
Fragments arranged by size:
2869 2580 187 110
BsrGI T'GTAC_A
Cuts at: 96 2283 96
Size: 2187 3559
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5746
BssSI C'ACGA_G
Cuts at: 2087 3806 4818 2087
Size: 1719 1012 3015
Fragments arranged by size:
3015 1719 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2062 794
Size: 1268 4478
BstBI TT'CG_AA
Cuts at: 4550 4550
Size: 5746
BstEII G'GTnAC_C
Cuts at: 1184 2672 1184
Size: 1488 4258
BstYI r'GATC_y
Cuts at: 1972 2337 2760 3076 3443 3828 3845 5297 5383
Size: 365 423 316 367 385 17 1452 86
Cuts at: 5383 5395 5741 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 423 385 367 365 346 316
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2134 2583 2652 513
Size: 552 1069 449 69 3607
Fragments arranged by size:
3607 1069 552 449 69
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5007
Fragments arranged by size:
5007 683 56
BtsI GCAGTG_nn'
Cuts at: 2900 3262 4056 4076 2900
Size: 362 794 20 4570
Fragments arranged by size:
4570 794 362 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5746
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5711
DraI TTT'AAA
Cuts at: 3050 3050
Size: 5746
DraIII CAC_nnn'GTG
Cuts at: 3010 3010
Size: 5746
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4753 809
Size: 507 3437 1802
Fragments arranged by size:
3437 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2122 3012 3193
Size: 54 1425 50 275 18 292 890 181
Cuts at: 3193 4082 5711 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 890 889 292 275 181 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3193 1537
Size: 275 1381 4090
Fragments arranged by size:
4090 1381 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2873 3676 1353
Size: 1520 803 3423
Fragments arranged by size:
3423 1520 803
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2249 4333 4707 4853 387
Size: 1064 138 660 2084 374 146 1280
Fragments arranged by size:
2084 1280 1064 660 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2345 2492 2680 2819 3809 4406 5193
Size: 393 713 147 188 139 990 597 787
Cuts at: 5193 5461 1239
Size: 268 1524
Fragments arranged by size:
1524 990 787 713 597 393 268 188
147 139
EcoO109I rG'GnC_Cy
Cuts at: 2171 2491 2171
Size: 320 5426
EcoRI G'AATT_C
Cuts at: 3055 3433 3055
Size: 378 5368
FspI TGC'GCA
Cuts at: 4252 4252
Size: 5746
HaeII r_GCGC'y
Cuts at: 1678 2583 4893 1678
Size: 905 2310 2531
Fragments arranged by size:
2531 2310 905
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2305 2337 2783 2815 2934
Size: 32 135 32 733 32 446 32 119
Cuts at: 2934 2966 2976 3008 4387 4419 4461 4493 1373
Size: 32 10 32 1379 32 42 32 2626
Fragments arranged by size:
2626 1379 733 446 135 119 42 32
32 32 32 32 32 32 32 10
HincII GTy'rAC
Cuts at: 669 1657 1938 3090 3336 669
Size: 988 281 1152 246 3079
Fragments arranged by size:
3079 1152 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5746
HpaI GTT'AAC
Cuts at: 3336 3336
Size: 5746
KpnI G_GTAC'C
Cuts at: 3071 3071
Size: 5746
MfeI C'AATT_G
Cuts at: 3345 3345
Size: 5746
MluI A'CGCG_T
Cuts at: 5622 5622
Size: 5746
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2172 2814 4860 5044 1305
Size: 867 642 2046 184 2007
Fragments arranged by size:
2046 2007 867 642 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3014 10
Size: 54 1425 343 1182 2742
Fragments arranged by size:
2742 1425 1182 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3032 3704 4063 4222 518
Size: 648 1866 672 359 159 2042
Fragments arranged by size:
2042 1866 672 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2572 3162 3839 4987 5232 1606
Size: 966 590 677 1148 245 2120
Fragments arranged by size:
2120 1148 966 677 590 245
NcoI C'CATG_G
Cuts at: 513 1065 2134 513
Size: 552 1069 4125
Fragments arranged by size:
4125 1069 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5746
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5746
NotI GC'GGCC_GC
Cuts at: 3193 3193
Size: 5746
NsiI A_TGCA'T
Cuts at: 2598 2598
Size: 5746
NspI r_CATG'y
Cuts at: 1132 1229 2103 2131 2596 2749 2959 3104 4649
Size: 97 874 28 465 153 210 145 1545
Cuts at: 4649 1132
Size: 2229
Fragments arranged by size:
2229 1545 874 465 210 153 145 97
28
PasI CC'CwG_GG
Cuts at: 1348 2167 1348
Size: 819 4927
PciI A'CATG_T
Cuts at: 2745 4645 2745
Size: 1900 3846
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2167 1108
Size: 1059 4687
PfoI T'CCnGG_A
Cuts at: 2538 2538
Size: 5746
PmeI GTTT'AAAC
Cuts at: 3050 3050
Size: 5746
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3815 3847 5328 5360 3815
Size: 32 1481 32 4201
Fragments arranged by size:
4201 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2491 2491
Size: 5746
PshAI GACnn'nnGTC
Cuts at: 1613 2679 1613
Size: 1066 4680
PsiI TTA'TAA
Cuts at: 3316 3316
Size: 5746
PspXI vC'TCGA_Gb
Cuts at: 2073 2073
Size: 5746
PstI C_TGCA'G
Cuts at: 830 3098 830
Size: 2268 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5746
PvuII CAG'CTG
Cuts at: 2572 2572
Size: 5746
SalI G'TCGA_C
Cuts at: 1936 3088 1936
Size: 1152 4594
SbfI CC_TGCA'GG
Cuts at: 3098 3098
Size: 5746
ScaI AGT'ACT
Cuts at: 2513 2561 3994 5706 2513
Size: 48 1433 1712 2553
Fragments arranged by size:
2553 1712 1433 48
SexAI A'CCwGG_T
Cuts at: 3006 3006
Size: 5746
SfcI C'TryA_G
Cuts at: 826 1045 3094 4229 4910 5101 826
Size: 219 2049 1135 681 191 1471
Fragments arranged by size:
2049 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5746
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5746
SmaI CCC'GGG
Cuts at: 1872 2917 3073 1872
Size: 1045 156 4545
Fragments arranged by size:
4545 1045 156
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2073 3850 4751 5013 820
Size: 197 926 130 1777 901 262 1553
Fragments arranged by size:
1777 1553 926 901 262 197 130
SnaBI TAC'GTA
Cuts at: 493 3537 493
Size: 3044 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5746
SphI G_CATG'C
Cuts at: 1132 2103 3104 1132
Size: 971 1001 3774
Fragments arranged by size:
3774 1001 971
SspI AAT'ATT
Cuts at: 5 52 3670 5
Size: 47 3618 2081
Fragments arranged by size:
3618 2081 47
StuI AGG'CCT
Cuts at: 1517 2071 1517
Size: 554 5192
StyI C'CwwG_G
Cuts at: 513 1065 2066 2134 2358 2677 2802 3185 513
Size: 552 1001 68 224 319 125 383 3074
Fragments arranged by size:
3074 1001 552 383 319 224 125 68
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2427 3773 3790 3943 4128 4573 325
Size: 1255 847 1346 17 153 185 445 1498
Fragments arranged by size:
1498 1346 1255 847 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2283 2511 2559
Size: 274 80 33 51 1443 306 228 48
Cuts at: 2559 3992 5704 96
Size: 1433 1712 138
Fragments arranged by size:
1712 1443 1433 306 274 228 138 80
51 48 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5706 5738 5706
Size: 32 5714
Tth111I GACn'n_nGTC
Cuts at: 1316 2375 1316
Size: 1059 4687
XbaI T'CTAG_A
Cuts at: 3082 3082
Size: 5746
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2132 2299 2132
Size: 167 5579
XhoI C'TCGA_G
Cuts at: 1943 2073 1943
Size: 130 5616
XmaI C'CCGG_G
Cuts at: 1870 2915 3071 1870
Size: 1045 156 4545
Fragments arranged by size:
4545 1045 156
XmnI GAAnn'nnTTC
Cuts at: 2867 3875 2867
Size: 1008 4738
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3554 276
Size: 53 83 186 936 2020 2468
Fragments arranged by size:
2468 2020 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspXI PstI PvuI PvuII SalI SbfI ScaI
SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoNI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI PacI PmlI PspOMI PsrI RsrII SacI SacII
SanDI SapI SfiI SfoI SrfI SwaI