Restriction map for
pFN21AB4774
(Circular) (Six-base) MAPSORT of: pFN21AB4774.seq Check: 3377 from: 1 to: 6895
pFN21AB4774 6895 bp
With 182 enzymes: SgfI *
October 20, 2009 10:36 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2375 4705 278
Size: 53 83 186 936 839 2330 2468
Fragments arranged by size:
2468 2330 936 839 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3294 4238 1937
Size: 1357 944 4594
Fragments arranged by size:
4594 1357 944
Acc65I G'GTAC_C
Cuts at: 4216 4216
Size: 6895
AclI AA'CG_TT
Cuts at: 1563 5022 5395 1563
Size: 3459 373 3063
Fragments arranged by size:
3459 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2774 4958 6342 6610 1632
Size: 1142 2184 1384 268 1917
Fragments arranged by size:
2184 1917 1384 1142 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6698
AflIII A'CryG_T
Cuts at: 1203 5794 6771 1203
Size: 4591 977 1327
Fragments arranged by size:
4591 1327 977
AgeI A'CCGG_T
Cuts at: 5726 5726
Size: 6895
AhdI GACnn_n'nnGTC
Cuts at: 5624 5624
Size: 6895
AleI CACnn'nnGTG
Cuts at: 1166 2490 1166
Size: 1324 5571
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3911 1184
Size: 2727 4168
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2819 2851 3083 3115 2819
Size: 32 232 32 6599
Fragments arranged by size:
6599 232 32 32
AlwNI CAG_nnn'CTG
Cuts at: 4638 6210 4638
Size: 1572 5323
ApaI G_GGCC'C
Cuts at: 2200 2591 2200
Size: 391 6504
ApaLI G'TGCA_C
Cuts at: 4952 6108 4952
Size: 1156 5739
ApoI r'AATT_y
Cuts at: 1493 3248 4160 4204 4430 4582 6691 1493
Size: 1755 912 44 226 152 2109 1697
Fragments arranged by size:
2109 1755 1697 912 226 152 44
AseI AT'TA_AT
Cuts at: 160 1032 5449 160
Size: 872 4417 1606
Fragments arranged by size:
4417 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6895
AvaI C'yCGr_G
Cuts at: 1870 1943 2036 3071 3731 3817 4016 4137 4220
Size: 73 93 1035 660 86 199 121 83
Cuts at: 4220 1870
Size: 4545
Fragments arranged by size:
4545 1035 660 199 121 93 86 83
73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2847 2880 2847
Size: 33 6862
BamHI G'GATC_C
Cuts at: 3396 4225 4592 3396
Size: 829 367 5699
Fragments arranged by size:
5699 829 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3172 3690 4216 5671 618
Size: 325 217 627 1385 518 526 1455 1842
Fragments arranged by size:
1842 1455 1385 627 526 518 325 217
BanII G_rGCy'C
Cuts at: 1391 2124 2200 2591 4214 1391
Size: 733 76 391 1623 4072
Fragments arranged by size:
4072 1623 733 391 76
BbeI G_GCGC'C
Cuts at: 3176 3176
Size: 6895
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2446 928
Size: 971 547 5377
Fragments arranged by size:
5377 971 547
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2222 2256 2804 2838 3449
Size: 34 50 34 201 34 548 34 611
Cuts at: 3449 3483 3502 3536 4118 4152 5086 5120 1903
Size: 34 19 34 582 34 934 34 3678
Fragments arranged by size:
3678 934 611 582 548 201 50 34
34 34 34 34 34 34 34 19
BciVI GTATCCnnnnn_n'
Cuts at: 2848 4787 5997 2848
Size: 1939 1210 3746
Fragments arranged by size:
3746 1939 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6895
BfrBI ATG'CAT
Cuts at: 2815 2815
Size: 6895
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5506 136
Size: 107 122 71 631 605 3834 1525
Fragments arranged by size:
3834 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6890 6890
Size: 6895
BlpI GC'TnA_GC
Cuts at: 2918 4312 2918
Size: 1394 5501
Bme1580I G_kGCm'C
Cuts at: 1790 2200 2591 2963 3501 4956 6112 1790
Size: 410 391 372 538 1455 1156 2573
Fragments arranged by size:
2573 1455 1156 538 410 391 372
BmgBI CAC'GTC
Cuts at: 1318 2656 3099 3567 1318
Size: 1338 443 468 4646
Fragments arranged by size:
4646 1338 468 443
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2485 3874 4027
Size: 416 53 83 399 24 1068 1389 153
Cuts at: 4027 4626 5584 442
Size: 599 958 1753
Fragments arranged by size:
1753 1389 1068 958 599 416 399 153
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6895
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2131 4140 2131
Size: 2009 4886
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2186 2525 2821 3184 3503 5555 1239
Size: 947 339 296 363 319 2052 2579
Fragments arranged by size:
2579 2052 947 363 339 319 296
Bpu10I CC'TnA_GC
Cuts at: 3210 3210
Size: 6895
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3569 5020 5885 6183 3569
Size: 1451 865 298 4281
Fragments arranged by size:
4281 1451 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2783 2969 5558 882
Size: 617 1284 186 2589 2219
Fragments arranged by size:
2589 2219 1284 617 186
BsaAI yAC'GTr
Cuts at: 493 1204 2569 3421 4686 493
Size: 711 1365 852 1265 2702
Fragments arranged by size:
2702 1365 1265 852 711
BsaBI GATnn'nnATC
Cuts at: 6889 6889
Size: 6895
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2372 3173 4144 4702
Size: 53 83 186 936 839 801 971 558
Cuts at: 4702 5084 5719 275
Size: 382 635 1451
Fragments arranged by size:
1451 971 936 839 801 635 558 382
186 83 53
BsaWI w'CCGG_w
Cuts at: 4182 5328 5726 6000 6147 6558 4182
Size: 1146 398 274 147 411 4519
Fragments arranged by size:
4519 1146 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2762 2792 2819 2849 2990
Size: 30 165 30 1359 30 27 30 141
Cuts at: 2990 3020 3875 3905 4046 4076 1178
Size: 30 855 30 141 30 3997
Fragments arranged by size:
3997 1359 855 165 141 141 30 30
30 30 30 30 30 27
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2120 2995 1186
Size: 934 875 5086
Fragments arranged by size:
5086 934 875
BseYI C'CCAG_C
Cuts at: 2288 2647 2962 3374 6098 6594 2288
Size: 359 315 412 2724 496 2589
Fragments arranged by size:
2724 2589 496 412 359 315
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3739 4190 1163
Size: 573 2003 451 3868
Fragments arranged by size:
3868 2003 573 451
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2065 2069 4345 5106 6134 1540
Size: 275 186 64 4 2276 761 1028 2301
Fragments arranged by size:
2301 2276 1028 761 275 186 64 4
BsiHKAI G_wGCw'C
Cuts at: 2303 3566 3659 4956 5041 6112 2303
Size: 1263 93 1297 85 1071 3086
Fragments arranged by size:
3086 1297 1263 1071 93 85
BsmI GAATG_Cn'
Cuts at: 4406 4499 4406
Size: 93 6802
BsmBI CGTCTCn'nnnn_
Cuts at: 3226 3598 3226
Size: 372 6523
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2124 2200 2303 2591 2963 3501 3566
Size: 399 334 76 103 288 372 538 65
Cuts at: 3566 3659 4214 4956 5041 6112 1391
Size: 93 555 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 555 538 399 372 334
288 103 93 85 76 65
BspEI T'CCGG_A
Cuts at: 6558 6558
Size: 6895
BspHI T'CATG_A
Cuts at: 4784 6514 4784
Size: 1730 5165
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4250 844
Size: 3406 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4782 1394
Size: 429 2959 3507
Fragments arranged by size:
3507 2959 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2169 2931 3931 5390 5564 65
Size: 1183 921 762 1000 1459 174 1396
Fragments arranged by size:
1459 1396 1183 1000 921 762 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2064 2358 3369 5539 5726 1700
Size: 110 254 294 1011 2170 187 2869
Fragments arranged by size:
2869 2170 1011 294 254 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6895
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6895
BssSI C'ACGA_G
Cuts at: 2642 4955 5967 2642
Size: 2313 1012 3570
Fragments arranged by size:
3570 2313 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3384 794
Size: 2590 4305
BstBI TT'CG_AA
Cuts at: 5699 5699
Size: 6895
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6895
BstYI r'GATC_y
Cuts at: 1972 2221 2348 3396 4225 4592 4977 4994 6446
Size: 249 127 1048 829 367 385 17 1452
Cuts at: 6446 6532 6544 6890 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1048 829 385 367 346 249
127 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2192 2766 2192
Size: 574 6321
BtgI C'CryG_G
Cuts at: 513 1065 2578 3670 513
Size: 552 1513 1092 3738
Fragments arranged by size:
3738 1513 1092 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6156
Fragments arranged by size:
6156 683 56
BtsI GCAGTG_nn'
Cuts at: 3043 3967 4411 5205 5225 3043
Size: 924 444 794 20 4713
Fragments arranged by size:
4713 924 794 444 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6895
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3982 4017 529
Size: 35 3418 35 3407
Fragments arranged by size:
3418 3407 35 35
DraI TTT'AAA
Cuts at: 4199 4199
Size: 6895
DraIII CAC_nnn'GTG
Cuts at: 3049 3049
Size: 6895
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5902 809
Size: 507 4586 1802
Fragments arranged by size:
4586 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2062 2066 2079
Size: 54 1425 50 275 18 232 4 13
Cuts at: 2079 2684 2902 3334 4342 5231 6860 8
Size: 605 218 432 1008 889 1629 43
Fragments arranged by size:
1629 1425 1008 889 605 432 275 232
218 54 50 43 18 13 4
EagI C'GGCC_G
Cuts at: 1537 1812 2062 2066 4342 1537
Size: 275 250 4 2276 4090
Fragments arranged by size:
4090 2276 275 250 4
EarI CTCTTCn'nnn_
Cuts at: 1353 2178 4825 1353
Size: 825 2647 3423
Fragments arranged by size:
3423 2647 825
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2117 3409 3795 5482 5856 6002
Size: 1064 138 528 1292 386 1687 374 146
Cuts at: 6002 387
Size: 1280
Fragments arranged by size:
1687 1292 1280 1064 528 386 374 146
138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2186 2525 2774 2821 3184 3503 4958
Size: 393 554 339 249 47 363 319 1455
Cuts at: 4958 5555 6342 6610 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1455 787 597 554 393 363 339
319 268 249 47
EcoNI CCTnn'n_nnAGG
Cuts at: 2102 2102
Size: 6895
EcoO109I rG'GnC_Cy
Cuts at: 2188 2196 2197 2587 2588 2188
Size: 8 1 390 1 6495
Fragments arranged by size:
6495 390 8 1 1
EcoRI G'AATT_C
Cuts at: 3248 4204 4582 3248
Size: 956 378 5561
Fragments arranged by size:
5561 956 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3301 3961 4117 3301
Size: 660 156 6079
Fragments arranged by size:
6079 660 156
FseI GG_CCGG'CC
Cuts at: 2068 2068
Size: 6895
FspI TGC'GCA
Cuts at: 3959 5401 3959
Size: 1442 5453
HaeII r_GCGC'y
Cuts at: 1678 3176 3457 3529 3760 6042 1678
Size: 1498 281 72 231 2282 2531
Fragments arranged by size:
2531 2282 1498 281 231 72
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2749 2762 2781 2794 3641
Size: 32 135 32 1177 13 19 13 847
Cuts at: 3641 3673 4046 4078 5536 5568 5610 5642 1373
Size: 32 373 32 1458 32 42 32 2626
Fragments arranged by size:
2626 1458 1177 847 373 135 42 32
32 32 32 32 32 19 13 13
HincII GTy'rAC
Cuts at: 669 1657 1938 3631 4239 4485 669
Size: 988 281 1693 608 246 3079
Fragments arranged by size:
3079 1693 988 608 281 246
HindIII A'AGCT_T
Cuts at: 748 2948 748
Size: 2200 4695
HpaI GTT'AAC
Cuts at: 4485 4485
Size: 6895
KasI G'GCGC_C
Cuts at: 3172 3172
Size: 6895
KpnI G_GTAC'C
Cuts at: 4220 4220
Size: 6895
MfeI C'AATT_G
Cuts at: 3265 4494 3265
Size: 1229 5666
MluI A'CGCG_T
Cuts at: 6771 6771
Size: 6895
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3253 4089 6009 6193 1305
Size: 1948 836 1920 184 2007
Fragments arranged by size:
2007 1948 1920 836 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2904 3336 10
Size: 54 1425 343 1072 432 3569
Fragments arranged by size:
3569 1425 1072 432 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2490 4049 4853 5212 5371 518
Size: 648 1324 1559 804 359 159 2042
Fragments arranged by size:
2042 1559 1324 804 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2029 2071 2292 2580 3465 3672 3688 3723
Size: 423 42 221 288 885 207 16 35
Cuts at: 3723 3762 3865 3974 4311 4988 6136 6381 1606
Size: 39 103 109 337 677 1148 245 2120
Fragments arranged by size:
2120 1148 885 677 423 337 288 245
221 207 109 103 42 39 35 16
NaeI GCC'GGC
Cuts at: 2066 2360 2066
Size: 294 6601
NarI GG'CG_CC
Cuts at: 3173 3173
Size: 6895
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6343
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6895
NgoMIV G'CCGG_C
Cuts at: 2064 2358 2064
Size: 294 6601
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6895
NotI GC'GGCC_GC
Cuts at: 4342 4342
Size: 6895
NsiI A_TGCA'T
Cuts at: 2817 2817
Size: 6895
NspI r_CATG'y
Cuts at: 1132 1229 3043 4048 4253 5798 1132
Size: 97 1814 1005 205 1545 2229
Fragments arranged by size:
2229 1814 1545 1005 205 97
PasI CC'CwG_GG
Cuts at: 1348 2782 3703 1348
Size: 1434 921 4540
Fragments arranged by size:
4540 1434 921
PciI A'CATG_T
Cuts at: 5794 5794
Size: 6895
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2647 2969 1108
Size: 1539 322 5034
Fragments arranged by size:
5034 1539 322
PfoI T'CCnGG_A
Cuts at: 2269 2502 2850 2269
Size: 233 348 6314
Fragments arranged by size:
6314 348 233
PmeI GTTT'AAAC
Cuts at: 4199 4199
Size: 6895
PmlI CAC'GTG
Cuts at: 3421 3421
Size: 6895
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2819 2851 4964 4996 6477 6509 2819
Size: 32 2113 32 1481 32 3205
Fragments arranged by size:
3205 2113 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2188 2188
Size: 6895
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6895
PsiI TTA'TAA
Cuts at: 4465 4465
Size: 6895
PspOMI G'GGCC_C
Cuts at: 2196 2587 2196
Size: 391 6504
PstI C_TGCA'G
Cuts at: 830 2266 3900 4131 4247 830
Size: 1436 1634 231 116 3478
Fragments arranged by size:
3478 1634 1436 231 116
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6895
PvuII CAG'CTG
Cuts at: 2292 3465 3723 3865 3974 2292
Size: 1173 258 142 109 5213
Fragments arranged by size:
5213 1173 258 142 109
SacII CC_GC'GG
Cuts at: 2581 3673 2581
Size: 1092 5803
SalI G'TCGA_C
Cuts at: 1936 4237 1936
Size: 2301 4594
SbfI CC_TGCA'GG
Cuts at: 4247 4247
Size: 6895
ScaI AGT'ACT
Cuts at: 5143 6855 5143
Size: 1712 5183
SfcI C'TryA_G
Cuts at: 826 1045 2262 3896 4127 4243 5378 6059 6250
Size: 219 1217 1634 231 116 1135 681 191
Cuts at: 6250 826
Size: 1471
Fragments arranged by size:
1634 1471 1217 1135 681 231 219 191
116
SfoI GGC'GCC
Cuts at: 3174 3174
Size: 6895
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6895
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6895
SmaI CCC'GGG
Cuts at: 1872 3073 3733 3819 4222 1872
Size: 1201 660 86 403 4545
Fragments arranged by size:
4545 1201 660 403 86
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3584 4999 5900 6162 820
Size: 197 926 1641 1415 901 262 1553
Fragments arranged by size:
1641 1553 1415 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4686 493
Size: 4193 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6895
SphI G_CATG'C
Cuts at: 1132 4253 1132
Size: 3121 3774
SrfI GCCC'GGGC
Cuts at: 3819 3819
Size: 6895
SspI AAT'ATT
Cuts at: 5 52 4819 5
Size: 47 4767 2081
Fragments arranged by size:
4767 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6895
StyI C'CwwG_G
Cuts at: 513 1065 3828 4334 513
Size: 552 2763 506 3074
Fragments arranged by size:
3074 2763 552 506
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3893 4922 4939 5092 5277 5722 325
Size: 1255 2313 1029 17 153 185 445 1498
Fragments arranged by size:
2313 1498 1255 1029 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4035 5141 6853
Size: 274 80 33 51 1443 2058 1106 1712
Cuts at: 6853 96
Size: 138
Fragments arranged by size:
2058 1712 1443 1106 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6855 6887 6855
Size: 32 6863
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6895
XbaI T'CTAG_A
Cuts at: 4231 4231
Size: 6895
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2388 2011
Size: 377 6518
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6895
XmaI C'CCGG_G
Cuts at: 1870 3071 3731 3817 4220 1870
Size: 1201 660 86 403 4545
Fragments arranged by size:
4545 1201 660 403 86
XmnI GAAnn'nnTTC
Cuts at: 2278 2831 5024 2278
Size: 553 2193 4149
Fragments arranged by size:
4149 2193 553
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2373 4703 276
Size: 53 83 186 936 839 2330 2468
Fragments arranged by size:
2468 2330 936 839 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NsiI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SacII SalI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BbvCI BsiWI BstXI BstZ17I
EcoICRI EcoRV FspAI NruI PacI PspXI PsrI RsrII
SacI SanDI SapI SexAI SfiI SwaI