Restriction map for
pFN21AB4757
(Circular) (Six-base) MAPSORT of: pFN21AB4757.seq Check: 1069 from: 1 to: 6337
pFN21AB4757 6337 bp
With 182 enzymes: SgfI *
February 27, 2009 14:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3356 3356
Size: 6337
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4147 278
Size: 53 83 186 936 2611 2468
Fragments arranged by size:
2611 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3680 1937
Size: 1743 4594
Acc65I G'GTAC_C
Cuts at: 3658 3658
Size: 6337
AclI AA'CG_TT
Cuts at: 1563 4464 4837 1563
Size: 2901 373 3063
Fragments arranged by size:
3063 2901 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2046 3571 4400 5784 6052 1632
Size: 414 1525 829 1384 268 1917
Fragments arranged by size:
1917 1525 1384 829 414 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6140
AflIII A'CryG_T
Cuts at: 1203 2351 2555 5236 6213 1203
Size: 1148 204 2681 977 1327
Fragments arranged by size:
2681 1327 1148 977 204
AgeI A'CCGG_T
Cuts at: 5168 5168
Size: 6337
AhdI GACnn_n'nnGTC
Cuts at: 5066 5066
Size: 6337
AleI CACnn'nnGTG
Cuts at: 1166 2624 2874 3462 3522 3546 3630 1166
Size: 1458 250 588 60 24 84 3873
Fragments arranged by size:
3873 1458 588 250 84 60 24
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6337
AlwNI CAG_nnn'CTG
Cuts at: 4080 5652 4080
Size: 1572 4765
ApaLI G'TGCA_C
Cuts at: 4394 5550 4394
Size: 1156 5181
ApoI r'AATT_y
Cuts at: 1493 2613 2697 2740 2781 2865 2949 3033 3117
Size: 1120 84 43 41 84 84 84 84
Cuts at: 3117 3369 3537 3646 3872 4024 6133 1493
Size: 252 168 109 226 152 2109 1697
Fragments arranged by size:
2109 1697 1120 252 226 168 152 109
84 84 84 84 84 43 41
AseI AT'TA_AT
Cuts at: 160 1032 4891 160
Size: 872 3859 1606
Fragments arranged by size:
3859 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6337
AvaI C'yCGr_G
Cuts at: 1870 1943 3662 1870
Size: 73 1719 4545
Fragments arranged by size:
4545 1719 73
AvrII C'CTAG_G
Cuts at: 2210 2210
Size: 6337
BamHI G'GATC_C
Cuts at: 3667 4034 3667
Size: 367 5970
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3276 3658 5113 618
Size: 325 217 627 1489 382 1455 1842
Fragments arranged by size:
1842 1489 1455 627 382 325 217
BanII G_rGCy'C
Cuts at: 1391 2507 3095 3656 1391
Size: 1116 588 561 4072
Fragments arranged by size:
4072 1116 588 561
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5366
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4528 4562 1903
Size: 34 2591 34 3678
Fragments arranged by size:
3678 2591 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4229 5439 4229
Size: 1210 5127
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6337
BfrBI ATG'CAT
Cuts at: 2183 2183
Size: 6337
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4948 136
Size: 107 122 71 631 605 3276 1525
Fragments arranged by size:
3276 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6332 6332
Size: 6337
BlpI GC'TnA_GC
Cuts at: 3754 3754
Size: 6337
Bme1580I G_kGCm'C
Cuts at: 1790 4398 5554 1790
Size: 2608 1156 2573
Fragments arranged by size:
2608 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 2354 1318
Size: 1036 5301
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2967 4068 5026
Size: 416 53 83 399 24 1550 1101 958
Cuts at: 5026 442
Size: 1753
Fragments arranged by size:
1753 1550 1101 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6337
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3114 3114
Size: 6337
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2108 2559 2643 2727 2811 3147 3315 3399
Size: 869 451 84 84 84 336 168 84
Cuts at: 3399 4997 1239
Size: 1598 2579
Fragments arranged by size:
2579 1598 869 451 336 168 84 84
84 84
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2323 4462 5327 5625 2323
Size: 2139 865 298 3035
Fragments arranged by size:
3035 2139 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2539 3211 5000 882
Size: 617 1040 672 1789 2219
Fragments arranged by size:
2219 1789 1040 672 617
BsaAI yAC'GTr
Cuts at: 493 1204 4128 493
Size: 711 2924 2702
Fragments arranged by size:
2924 2702 711
BsaBI GATnn'nnATC
Cuts at: 2671 6331 2671
Size: 3660 2677
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4144 4526 5161 275
Size: 53 83 186 936 2611 382 635 1451
Fragments arranged by size:
2611 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4770 5168 5442 5589 6000 4770
Size: 398 274 147 411 5107
Fragments arranged by size:
5107 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6112
Fragments arranged by size:
6112 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6337
BseYI C'CCAG_C
Cuts at: 2061 5540 6036 2061
Size: 3479 496 2362
Fragments arranged by size:
3479 2362 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2434 1163
Size: 573 698 5066
Fragments arranged by size:
5066 698 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3787 4548 5576 1540
Size: 275 186 1786 761 1028 2301
Fragments arranged by size:
2301 1786 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2507 3179 4398 4483 5554 2507
Size: 672 1219 85 1071 3290
Fragments arranged by size:
3290 1219 1071 672 85
BsmI GAATG_Cn'
Cuts at: 2828 3848 3941 2828
Size: 1020 93 5224
Fragments arranged by size:
5224 1020 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2360 2360
Size: 6337
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2507 3095 3179 3656 4398 4483 5554
Size: 399 717 588 84 477 742 85 1071
Cuts at: 5554 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 717 588 477 399 85
84
BspEI T'CCGG_A
Cuts at: 6000 6000
Size: 6337
BspHI T'CATG_A
Cuts at: 4226 5956 4226
Size: 1730 4607
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3356 3692 844
Size: 2512 336 3489
Fragments arranged by size:
3489 2512 336
BsrBI CCG'CTC
Cuts at: 1394 1823 4224 1394
Size: 429 2401 3507
Fragments arranged by size:
3507 2401 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2734 3322 4832 5006 65
Size: 1183 1486 588 1510 174 1396
Fragments arranged by size:
1510 1486 1396 1183 588 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4981 5168 1700
Size: 110 3171 187 2869
Fragments arranged by size:
3171 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6337
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6337
BssSI C'ACGA_G
Cuts at: 4397 5409 4397
Size: 1012 5325
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6337
BstBI TT'CG_AA
Cuts at: 2917 5141 2917
Size: 2224 4113
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6337
BstYI r'GATC_y
Cuts at: 1972 3667 4034 4419 4436 5888 5974 5986 6332
Size: 1695 367 385 17 1452 86 12 346
Cuts at: 6332 1972
Size: 1977
Fragments arranged by size:
1977 1695 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5785
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5598
Fragments arranged by size:
5598 683 56
BtsI GCAGTG_nn'
Cuts at: 2654 2902 3238 3853 4647 4667 2654
Size: 248 336 615 794 20 4324
Fragments arranged by size:
4324 794 615 336 248 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6337
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2716 2751 2800 2835 3304 3339 529
Size: 35 2152 35 49 35 469 35 3527
Fragments arranged by size:
3527 2152 469 49 35 35 35 35
DraI TTT'AAA
Cuts at: 3641 3641
Size: 6337
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5344 809
Size: 507 4028 1802
Fragments arranged by size:
4028 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3784 4673 6302
Size: 54 1425 50 275 18 1954 889 1629
Cuts at: 6302 8
Size: 43
Fragments arranged by size:
1954 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3784 1537
Size: 275 1972 4090
Fragments arranged by size:
4090 1972 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2022 2038 2367 2914 4267 1353
Size: 669 16 329 547 1353 3423
Fragments arranged by size:
3423 1353 669 547 329 16
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4924 5298 5444 387
Size: 1064 138 3335 374 146 1280
Fragments arranged by size:
3335 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2505 2505
Size: 6337
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2046 2108 2559 2643 2727 2811 3147
Size: 393 414 62 451 84 84 84 336
Cuts at: 3147 3315 3399 3571 4400 4997 5784 6052 1239
Size: 168 84 172 829 597 787 268 1524
Fragments arranged by size:
1524 829 787 597 451 414 393 336
268 172 168 84 84 84 84 62
EcoNI CCTnn'n_nnAGG
Cuts at: 2237 2237
Size: 6337
EcoRI G'AATT_C
Cuts at: 2613 2697 2740 2781 2865 2949 3033 3117 3369
Size: 84 43 41 84 84 84 84 252
Cuts at: 3369 3537 3646 4024 2613
Size: 168 109 378 4926
Fragments arranged by size:
4926 378 252 168 109 84 84 84
84 84 43 41
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2067 2067
Size: 6337
FspI TGC'GCA
Cuts at: 4843 4843
Size: 6337
HaeII r_GCGC'y
Cuts at: 1678 5484 1678
Size: 3806 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2368 2400 4978 5010 5052
Size: 32 135 32 796 32 2578 32 42
Cuts at: 5052 5084 1373
Size: 32 2626
Fragments arranged by size:
2626 2578 796 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3681 3927 669
Size: 988 281 1743 246 3079
Fragments arranged by size:
3079 1743 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2323 748
Size: 1575 4762
HpaI GTT'AAC
Cuts at: 3927 3927
Size: 6337
KpnI G_GTAC'C
Cuts at: 3662 3662
Size: 6337
MfeI C'AATT_G
Cuts at: 3936 3936
Size: 6337
MluI A'CGCG_T
Cuts at: 6213 6213
Size: 6337
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2465 5451 5635 1305
Size: 1160 2986 184 2007
Fragments arranged by size:
2986 2007 1160 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4515
Fragments arranged by size:
4515 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2624 2638 2665 2874 2976 3142 3462
Size: 648 1458 14 27 209 102 166 320
Cuts at: 3462 3522 3546 3630 4295 4654 4813 518
Size: 60 24 84 665 359 159 2042
Fragments arranged by size:
2042 1458 665 648 359 320 209 166
159 102 84 60 27 24 14
MspA1I CmG'CkG
Cuts at: 1606 3753 4430 5578 5823 1606
Size: 2147 677 1148 245 2120
Fragments arranged by size:
2147 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5785
NdeI CA'TA_TG
Cuts at: 387 2635 2891 3363 387
Size: 2248 256 472 3361
Fragments arranged by size:
3361 2248 472 256
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6337
NotI GC'GGCC_GC
Cuts at: 3784 3784
Size: 6337
NsiI A_TGCA'T
Cuts at: 2185 2185
Size: 6337
NspI r_CATG'y
Cuts at: 1132 1229 2187 2559 3695 5240 1132
Size: 97 958 372 1136 1545 2229
Fragments arranged by size:
2229 1545 1136 958 372 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6337
PciI A'CATG_T
Cuts at: 2555 5236 2555
Size: 2681 3656
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6337
PfoI T'CCnGG_A
Cuts at: 2238 2238
Size: 6337
PmeI GTTT'AAAC
Cuts at: 3641 3641
Size: 6337
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4406 4438 5919 5951 4406
Size: 32 1481 32 4792
Fragments arranged by size:
4792 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6337
PsiI TTA'TAA
Cuts at: 2721 2805 3907 2721
Size: 84 1102 5151
Fragments arranged by size:
5151 1102 84
PstI C_TGCA'G
Cuts at: 830 3689 830
Size: 2859 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6337
SacI G_AGCT'C
Cuts at: 2507 2507
Size: 6337
SalI G'TCGA_C
Cuts at: 1936 3679 1936
Size: 1743 4594
SapI GCTCTTCn'nnn_
Cuts at: 2914 2914
Size: 6337
SbfI CC_TGCA'GG
Cuts at: 3689 3689
Size: 6337
ScaI AGT'ACT
Cuts at: 4585 6297 4585
Size: 1712 4625
SfcI C'TryA_G
Cuts at: 826 1045 2367 3685 4820 5501 5692 826
Size: 219 1322 1318 1135 681 191 1471
Fragments arranged by size:
1471 1322 1318 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6337
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6337
SmaI CCC'GGG
Cuts at: 1872 3664 1872
Size: 1792 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2338 4441 5342 5604 820
Size: 197 926 395 2103 901 262 1553
Fragments arranged by size:
2103 1553 926 901 395 262 197
SnaBI TAC'GTA
Cuts at: 493 4128 493
Size: 3635 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6337
SphI G_CATG'C
Cuts at: 1132 3695 1132
Size: 2563 3774
SspI AAT'ATT
Cuts at: 5 52 4261 5
Size: 47 4209 2081
Fragments arranged by size:
4209 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6337
StyI C'CwwG_G
Cuts at: 513 1065 2210 3776 513
Size: 552 1145 1566 3074
Fragments arranged by size:
3074 1566 1145 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4364 4381 4534 4719 5164 325
Size: 1255 2784 17 153 185 445 1498
Fragments arranged by size:
2784 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4583 6295 96
Size: 274 80 33 51 1443 2606 1712 138
Fragments arranged by size:
2606 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6297 6329 6297
Size: 32 6305
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6337
XbaI T'CTAG_A
Cuts at: 3673 3673
Size: 6337
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6337
XmaI C'CCGG_G
Cuts at: 1870 3662 1870
Size: 1792 4545
XmnI GAAnn'nnTTC
Cuts at: 2744 2828 2996 3500 4466 2744
Size: 84 168 504 966 4615
Fragments arranged by size:
4615 966 504 168 84
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4145 276
Size: 53 83 186 936 2611 2468
Fragments arranged by size:
2611 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PpiI PshAI PsiI PstI PvuI SacI SalI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI BaeI BbeI BbvCI Bpu10I
BsiWI BstXI BstZ17I Bsu36I DraIII EcoO109I EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI
PpuMI PspOMI PspXI PsrI PvuII RsrII SacII SanDI
SexAI SfiI SfoI SrfI SwaI XcmI