Restriction map for
pFN21AB4740
(Circular) (Six-base) MAPSORT of: pFN21AB4740.seq Check: 9862 from: 1 to: 7315
pFN21AB4740 7315 bp
With 182 enzymes: SgfI *
April 15, 2010 09:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2782 4336 4523 2782
Size: 1554 187 5574
Fragments arranged by size:
5574 1554 187
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2768 5125 278
Size: 53 83 186 936 1232 2357 2468
Fragments arranged by size:
2468 2357 1232 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4658 1937
Size: 2721 4594
Acc65I G'GTAC_C
Cuts at: 4403 4636 4403
Size: 233 7082
AclI AA'CG_TT
Cuts at: 1563 5442 5815 1563
Size: 3879 373 3063
Fragments arranged by size:
3879 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2586 2862 3145 4085 5378 6762 7030 1632
Size: 954 276 283 940 1293 1384 268 1917
Fragments arranged by size:
1917 1384 1293 954 940 283 276 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7118
AflIII A'CryG_T
Cuts at: 1203 2947 6214 7191 1203
Size: 1744 3267 977 1327
Fragments arranged by size:
3267 1744 1327 977
AgeI A'CCGG_T
Cuts at: 4400 6146 4400
Size: 1746 5569
AhdI GACnn_n'nnGTC
Cuts at: 3937 6044 3937
Size: 2107 5208
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7315
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7315
AlwNI CAG_nnn'CTG
Cuts at: 2732 2888 5058 6630 2732
Size: 156 2170 1572 3417
Fragments arranged by size:
3417 2170 1572 156
ApaI G_GGCC'C
Cuts at: 3221 3221
Size: 7315
ApaLI G'TGCA_C
Cuts at: 5372 6528 5372
Size: 1156 6159
ApoI r'AATT_y
Cuts at: 1493 4624 4850 5002 7111 1493
Size: 3131 226 152 2109 1697
Fragments arranged by size:
3131 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5869 160
Size: 872 4837 1606
Fragments arranged by size:
4837 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7315
AvaI C'yCGr_G
Cuts at: 1870 1943 3030 3433 4146 4640 1870
Size: 73 1087 403 713 494 4545
Fragments arranged by size:
4545 1087 713 494 403 73
BamHI G'GATC_C
Cuts at: 3304 3424 4377 4645 5012 3304
Size: 120 953 268 367 5607
Fragments arranged by size:
5607 953 367 268 120
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2294 2538 2655 4403 4636
Size: 325 217 627 507 244 117 1748 233
Cuts at: 4636 6091 618
Size: 1455 1842
Fragments arranged by size:
1842 1748 1455 627 507 325 244 233
217 117
BanII G_rGCy'C
Cuts at: 1391 2192 3221 4634 1391
Size: 801 1029 1413 4072
Fragments arranged by size:
4072 1413 1029 801
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2174 3177 928
Size: 971 275 1003 5066
Fragments arranged by size:
5066 1003 971 275
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3820 3854 5506 5540 1903
Size: 34 1883 34 1652 34 3678
Fragments arranged by size:
3678 1883 1652 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5207 6417 5207
Size: 1210 6105
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7315
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2688 2837 5926
Size: 107 122 71 631 605 1016 149 3089
Cuts at: 5926 136
Size: 1525
Fragments arranged by size:
3089 1525 1016 631 605 149 122 107
71
BglII A'GATC_T
Cuts at: 7310 7310
Size: 7315
BlpI GC'TnA_GC
Cuts at: 4732 4732
Size: 7315
Bme1580I G_kGCm'C
Cuts at: 1790 2473 3221 5376 6532 1790
Size: 683 748 2155 1156 2573
Fragments arranged by size:
2573 2155 1156 748 683
BmgBI CAC'GTC
Cuts at: 1318 2948 1318
Size: 1630 5685
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2148 2181 3289
Size: 416 53 83 399 24 731 33 1108
Cuts at: 3289 4049 5046 6004 442
Size: 760 997 958 1753
Fragments arranged by size:
1753 1108 997 958 760 731 416 399
83 53 33 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7315
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2281 2491 2511 2988 3125 3956 4028 4226
Size: 1042 210 20 477 137 831 72 198
Cuts at: 4226 4259 5975 1239
Size: 33 1716 2579
Fragments arranged by size:
2579 1716 1042 831 477 210 198 137
72 33 20
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2296 2806 5440 6305 6603 2296
Size: 510 2634 865 298 3008
Fragments arranged by size:
3008 2634 865 510 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2153 3910 4586 5978 882
Size: 617 654 1757 676 1392 2219
Fragments arranged by size:
2219 1757 1392 676 654 617
BsaAI yAC'GTr
Cuts at: 493 1204 2128 3100 3271 3547 5106 493
Size: 711 924 972 171 276 1559 2702
Fragments arranged by size:
2702 1559 972 924 711 276 171
BsaBI GATnn'nnATC
Cuts at: 7309 7309
Size: 7315
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2765 5122 5504 6139
Size: 53 83 186 936 1232 2357 382 635
Cuts at: 6139 275
Size: 1451
Fragments arranged by size:
2357 1451 1232 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4400 5748 6146 6420 6567 6978 4400
Size: 1348 398 274 147 411 4737
Fragments arranged by size:
4737 1348 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2862 2892 3770 3800 1178
Size: 30 165 30 1459 30 878 30 4693
Fragments arranged by size:
4693 1459 878 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3671 3778 4112 4604 1186
Size: 2485 107 334 492 3897
Fragments arranged by size:
3897 2485 492 334 107
BseYI C'CCAG_C
Cuts at: 2549 2617 2827 2855 2881 3009 3234 6518 7014
Size: 68 210 28 26 128 225 3284 496
Cuts at: 7014 2549
Size: 2850
Fragments arranged by size:
3284 2850 496 225 210 128 68 28
26
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2763 1163
Size: 573 1027 5715
Fragments arranged by size:
5715 1027 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3090 4765 5526 6554 1540
Size: 275 186 1089 1675 761 1028 2301
Fragments arranged by size:
2301 1675 1089 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2192 2647 3720 4295 5376 5461 6532 2192
Size: 455 1073 575 1081 85 1071 2975
Fragments arranged by size:
2975 1081 1073 1071 575 455 85
BsmI GAATG_Cn'
Cuts at: 4826 4919 4826
Size: 93 7222
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2192 2473 2647 3221 3720 4295 4634
Size: 399 402 281 174 574 499 575 339
Cuts at: 4634 5376 5461 6532 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 575 574 499 402 399
339 281 174 85
BspEI T'CCGG_A
Cuts at: 6978 6978
Size: 7315
BspHI T'CATG_A
Cuts at: 3600 5204 6934 3600
Size: 1604 1730 3981
Fragments arranged by size:
3981 1730 1604
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2379 2782 3027 3630 4336 4415 4523 4670
Size: 1535 403 245 603 706 79 108 147
Cuts at: 4670 844
Size: 3489
Fragments arranged by size:
3489 1535 706 603 403 245 147 108
79
BsrBI CCG'CTC
Cuts at: 1394 1823 2992 5202 1394
Size: 429 1169 2210 3507
Fragments arranged by size:
3507 2210 1169 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5810 5984 65
Size: 1183 4562 174 1396
Fragments arranged by size:
4562 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2535 2624 2652 2836 3630 4302 4400
Size: 110 725 89 28 184 794 672 98
Cuts at: 4400 5959 6146 1700
Size: 1559 187 2869
Fragments arranged by size:
2869 1559 794 725 672 187 184 110
98 89 28
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7315
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7315
BssSI C'ACGA_G
Cuts at: 3710 4058 5375 6387 3710
Size: 348 1317 1012 4638
Fragments arranged by size:
4638 1317 1012 348
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2559 794
Size: 1765 5550
BstBI TT'CG_AA
Cuts at: 3579 6119 3579
Size: 2540 4775
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7315
BstYI r'GATC_y
Cuts at: 1972 2573 3025 3304 3424 4329 4377 4531 4645
Size: 601 452 279 120 905 48 154 114
Cuts at: 4645 5012 5397 5414 6866 6952 6964 7310 1972
Size: 367 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 905 601 452 385 367 346
279 154 120 114 86 48 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 3090 3843 3856 513
Size: 552 939 1086 753 13 3972
Fragments arranged by size:
3972 1086 939 753 552 13
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3664 3823 487
Size: 683 56 2438 159 3979
Fragments arranged by size:
3979 2438 683 159 56
BtsI GCAGTG_nn'
Cuts at: 2473 3122 4831 5625 5645 2473
Size: 649 1709 794 20 4143
Fragments arranged by size:
4143 1709 794 649 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7315
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7280
DraI TTT'AAA
Cuts at: 2253 4619 2253
Size: 2366 4949
DraIII CAC_nnn'GTG
Cuts at: 3715 3715
Size: 7315
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6322 809
Size: 507 5006 1802
Fragments arranged by size:
5006 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2694 4762 5651
Size: 54 1425 50 275 18 864 2068 889
Cuts at: 5651 7280 8
Size: 1629 43
Fragments arranged by size:
2068 1629 1425 889 864 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4762 1537
Size: 275 2950 4090
Fragments arranged by size:
4090 2950 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4104 4152 4283 4367 5245 1353
Size: 2751 48 131 84 878 3423
Fragments arranged by size:
3423 2751 878 131 84 48
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2421 5902 6276 6422 387
Size: 1064 138 832 3481 374 146 1280
Fragments arranged by size:
3481 1280 1064 832 374 146 138
EcoICRI GAG'CTC
Cuts at: 2190 2190
Size: 7315
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2281 2491 2511 2586 2862 2988 3125
Size: 393 649 210 20 75 276 126 137
Cuts at: 3125 3145 3956 4028 4085 4226 4259 5378 5975
Size: 20 811 72 57 141 33 1119 597
Cuts at: 5975 6762 7030 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1119 811 787 649 597 393 276
268 210 141 137 126 75 72 57
33 20 20
EcoO109I rG'GnC_Cy
Cuts at: 2896 3218 3661 2896
Size: 322 443 6550
Fragments arranged by size:
6550 443 322
EcoRI G'AATT_C
Cuts at: 4624 5002 4624
Size: 378 6937
FspI TGC'GCA
Cuts at: 3104 5821 3104
Size: 2717 4598
HaeII r_GCGC'y
Cuts at: 1678 3851 6462 1678
Size: 2173 2611 2531
Fragments arranged by size:
2611 2531 2173
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2785 2817 3923 3955 5956
Size: 32 135 32 1213 32 1106 32 2001
Cuts at: 5956 5988 6030 6062 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2001 1213 1106 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2108 2308 2962 4659 4905 669
Size: 988 281 170 200 654 1697 246 3079
Fragments arranged by size:
3079 1697 988 654 281 246 200 170
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7315
HpaI GTT'AAC
Cuts at: 4905 4905
Size: 7315
KpnI G_GTAC'C
Cuts at: 4407 4640 4407
Size: 233 7082
MfeI C'AATT_G
Cuts at: 4914 4914
Size: 7315
MluI A'CGCG_T
Cuts at: 7191 7191
Size: 7315
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2340 3110 3616 6429 6613 1305
Size: 1035 770 506 2813 184 2007
Fragments arranged by size:
2813 2007 1035 770 506 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2696 10
Size: 54 1425 343 864 4629
Fragments arranged by size:
4629 1425 864 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2595 3528 3605 3644 3842 5273 5632
Size: 648 1429 933 77 39 198 1431 359
Cuts at: 5632 5791 518
Size: 159 2042
Fragments arranged by size:
2042 1431 1429 933 648 359 198 159
77 39
MspA1I CmG'CkG
Cuts at: 1606 2359 2491 2885 3092 3130 4204 4561 4731
Size: 753 132 394 207 38 1074 357 170
Cuts at: 4731 5408 6556 6801 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 1074 753 677 394 357 245
207 170 132 38
NaeI GCC'GGC
Cuts at: 2654 3632 2654
Size: 978 6337
NcoI C'CATG_G
Cuts at: 513 1065 2004 3843 3856 513
Size: 552 939 1839 13 3972
Fragments arranged by size:
3972 1839 939 552 13
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7315
NgoMIV G'CCGG_C
Cuts at: 2652 3630 2652
Size: 978 6337
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7315
NotI GC'GGCC_GC
Cuts at: 4762 4762
Size: 7315
NspI r_CATG'y
Cuts at: 1132 1229 2356 4261 4673 6218 1132
Size: 97 1127 1905 412 1545 2229
Fragments arranged by size:
2229 1905 1545 1127 412 97
PasI CC'CwG_GG
Cuts at: 1348 3214 3316 1348
Size: 1866 102 5347
Fragments arranged by size:
5347 1866 102
PciI A'CATG_T
Cuts at: 6214 6214
Size: 7315
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2985 3075 3690 3972 4030 1108
Size: 1877 90 615 282 58 4393
Fragments arranged by size:
4393 1877 615 282 90 58
PfoI T'CCnGG_A
Cuts at: 2424 2576 3327 4023 4176 2424
Size: 152 751 696 153 5563
Fragments arranged by size:
5563 751 696 153 152
PmeI GTTT'AAAC
Cuts at: 4619 4619
Size: 7315
PmlI CAC'GTG
Cuts at: 3547 3547
Size: 7315
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5384 5416 6897 6929 5384
Size: 32 1481 32 5770
Fragments arranged by size:
5770 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7315
PsiI TTA'TAA
Cuts at: 4885 4885
Size: 7315
PspOMI G'GGCC_C
Cuts at: 3217 3217
Size: 7315
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3491 3523 3491
Size: 32 7283
PstI C_TGCA'G
Cuts at: 830 2376 3774 4392 4667 830
Size: 1546 1398 618 275 3478
Fragments arranged by size:
3478 1546 1398 618 275
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7315
PvuII CAG'CTG
Cuts at: 2359 2491 4561 2359
Size: 132 2070 5113
Fragments arranged by size:
5113 2070 132
SacI G_AGCT'C
Cuts at: 2192 2192
Size: 7315
SacII CC_GC'GG
Cuts at: 3093 3093
Size: 7315
SalI G'TCGA_C
Cuts at: 1936 4657 1936
Size: 2721 4594
SapI GCTCTTCn'nnn_
Cuts at: 4152 4283 4152
Size: 131 7184
SbfI CC_TGCA'GG
Cuts at: 4667 4667
Size: 7315
ScaI AGT'ACT
Cuts at: 5563 7275 5563
Size: 1712 5603
SfcI C'TryA_G
Cuts at: 826 1045 2372 3770 4388 4663 5798 6479 6670
Size: 219 1327 1398 618 275 1135 681 191
Cuts at: 6670 826
Size: 1471
Fragments arranged by size:
1471 1398 1327 1135 681 618 275 219
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7315
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7315
SmaI CCC'GGG
Cuts at: 1872 3435 4642 1872
Size: 1563 1207 4545
Fragments arranged by size:
4545 1563 1207
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2275 2785 5419 6320 6582 820
Size: 197 926 332 510 2634 901 262 1553
Fragments arranged by size:
2634 1553 926 901 510 332 262 197
SnaBI TAC'GTA
Cuts at: 493 5106 493
Size: 4613 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7315
SphI G_CATG'C
Cuts at: 1132 4261 4673 1132
Size: 3129 412 3774
Fragments arranged by size:
3774 3129 412
SspI AAT'ATT
Cuts at: 5 52 5239 5
Size: 47 5187 2081
Fragments arranged by size:
5187 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7315
StyI C'CwwG_G
Cuts at: 513 1065 2004 2091 3843 3856 4754 513
Size: 552 939 87 1752 13 898 3074
Fragments arranged by size:
3074 1752 939 898 552 87 13
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4610 5342 5359 5512 5697 6142 325
Size: 1255 3030 732 17 153 185 445 1498
Fragments arranged by size:
3030 1498 1255 732 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4014 4113 5561
Size: 274 80 33 51 1443 2037 99 1448
Cuts at: 5561 7273 96
Size: 1712 138
Fragments arranged by size:
2037 1712 1448 1443 274 138 99 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2862 2894 7275 7307 2862
Size: 32 4381 32 2870
Fragments arranged by size:
4381 2870 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7315
XbaI T'CTAG_A
Cuts at: 4651 4651
Size: 7315
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4174 4174
Size: 7315
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7315
XmaI C'CCGG_G
Cuts at: 1870 3433 4640 1870
Size: 1563 1207 4545
Fragments arranged by size:
4545 1563 1207
XmnI GAAnn'nnTTC
Cuts at: 5444 5444
Size: 7315
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2766 5123 276
Size: 53 83 186 936 1232 2357 2468
Fragments arranged by size:
2468 2357 1232 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI
SacII SalI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoNI
EcoRV FalI FseI FspAI KasI NarI NruI NsiI
PacI PpuMI PspXI RsrII SanDI SexAI SfiI SfoI
SrfI SwaI