Restriction map for
pFN21AB4735
(Circular) (Six-base) MAPSORT of: pFN21AB4735.seq Check: 5705 from: 1 to: 5857
pFN21AB4735 5857 bp
With 182 enzymes: SgfI *
February 27, 2009 14:37 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3667 278
Size: 53 83 186 936 2131 2468
Fragments arranged by size:
2468 2131 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3200 1937
Size: 1263 4594
Acc65I G'GTAC_C
Cuts at: 3178 3178
Size: 5857
AclI AA'CG_TT
Cuts at: 1563 3156 3984 4357 1563
Size: 1593 828 373 3063
Fragments arranged by size:
3063 1593 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2002 2854 3920 5304 5572 1632
Size: 370 852 1066 1384 268 1917
Fragments arranged by size:
1917 1384 1066 852 370 268
AflII C'TTAA_G
Cuts at: 820 1017 2189 820
Size: 197 1172 4488
Fragments arranged by size:
4488 1172 197
AflIII A'CryG_T
Cuts at: 1203 2914 4756 5733 1203
Size: 1711 1842 977 1327
Fragments arranged by size:
1842 1711 1327 977
AgeI A'CCGG_T
Cuts at: 4688 4688
Size: 5857
AhdI GACnn_n'nnGTC
Cuts at: 4586 4586
Size: 5857
AleI CACnn'nnGTG
Cuts at: 1166 3053 1166
Size: 1887 3970
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2800 1184
Size: 1616 4241
AlwNI CAG_nnn'CTG
Cuts at: 3600 5172 3600
Size: 1572 4285
ApaLI G'TGCA_C
Cuts at: 3914 5070 3914
Size: 1156 4701
ApoI r'AATT_y
Cuts at: 1493 2471 2894 3006 3029 3166 3392 3544 5653
Size: 978 423 112 23 137 226 152 2109
Cuts at: 5653 1493
Size: 1697
Fragments arranged by size:
2109 1697 978 423 226 152 137 112
23
AseI AT'TA_AT
Cuts at: 160 1032 4411 160
Size: 872 3379 1606
Fragments arranged by size:
3379 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5857
AvaI C'yCGr_G
Cuts at: 1870 1943 3182 1870
Size: 73 1239 4545
Fragments arranged by size:
4545 1239 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2177 2210 2177
Size: 33 5824
BamHI G'GATC_C
Cuts at: 2596 3187 3554 2596
Size: 591 367 4899
Fragments arranged by size:
4899 591 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3178 4633 618
Size: 325 217 627 1391 1455 1842
Fragments arranged by size:
1842 1455 1391 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2291 2864 3176 1391
Size: 900 573 312 4072
Fragments arranged by size:
4072 900 573 312
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4886
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4048 4082 1903
Size: 34 2111 34 3678
Fragments arranged by size:
3678 2111 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3749 4959 3749
Size: 1210 4647
BclI T'GATC_A
Cuts at: 1548 2953 1548
Size: 1405 4452
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4468 136
Size: 107 122 71 631 605 2796 1525
Fragments arranged by size:
2796 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2160 5852 2160
Size: 3692 2165
BlpI GC'TnA_GC
Cuts at: 3274 3274
Size: 5857
Bme1580I G_kGCm'C
Cuts at: 1790 2881 3918 5074 1790
Size: 1091 1037 1156 2573
Fragments arranged by size:
2573 1156 1091 1037
BmgBI CAC'GTC
Cuts at: 1318 2797 1318
Size: 1479 4378
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3588 4546 442
Size: 416 53 83 399 24 2171 958 1753
Fragments arranged by size:
2171 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5857
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2305 2878 3125 4517 1239
Size: 1066 573 247 1392 2579
Fragments arranged by size:
2579 1392 1066 573 247
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2122 3982 4847 5145 2122
Size: 1860 865 298 2834
Fragments arranged by size:
2834 1860 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4520 882
Size: 617 3021 2219
Fragments arranged by size:
3021 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2917 3648 493
Size: 711 1713 731 2702
Fragments arranged by size:
2702 1713 731 711
BsaBI GATnn'nnATC
Cuts at: 5851 5851
Size: 5857
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3664 4046 4681 275
Size: 53 83 186 936 2131 382 635 1451
Fragments arranged by size:
2131 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4290 4688 4962 5109 5520 4290
Size: 398 274 147 411 4627
Fragments arranged by size:
4627 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2024 2039 2054 2069 1178
Size: 30 165 30 621 15 15 15 4966
Fragments arranged by size:
4966 621 165 30 30 15 15 15
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5857
BseYI C'CCAG_C
Cuts at: 2569 2996 3042 5060 5556 2569
Size: 427 46 2018 496 2870
Fragments arranged by size:
2870 2018 496 427 46
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2278 2543 1163
Size: 573 542 265 4477
Fragments arranged by size:
4477 573 542 265
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3307 4068 5096 1540
Size: 275 186 1306 761 1028 2301
Fragments arranged by size:
2301 1306 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3105 3918 4003 5074 3105
Size: 813 85 1071 3888
Fragments arranged by size:
3888 1071 813 85
BsmI GAATG_Cn'
Cuts at: 3368 3461 3368
Size: 93 5764
BsmBI CGTCTCn'nnnn_
Cuts at: 2424 2424
Size: 5857
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2291 2864 2881 3105 3176 3918 4003
Size: 399 501 573 17 224 71 742 85
Cuts at: 4003 5074 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 573 501 399 224 85
71 17
BspEI T'CCGG_A
Cuts at: 5520 5520
Size: 5857
BspHI T'CATG_A
Cuts at: 2457 3746 5476 2457
Size: 1289 1730 2838
Fragments arranged by size:
2838 1730 1289
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2852 3212 844
Size: 2008 360 3489
Fragments arranged by size:
3489 2008 360
BsrBI CCG'CTC
Cuts at: 1394 1823 3744 1394
Size: 429 1921 3507
Fragments arranged by size:
3507 1921 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4352 4526 65
Size: 1183 3104 174 1396
Fragments arranged by size:
3104 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4501 4688 1700
Size: 110 2691 187 2869
Fragments arranged by size:
2869 2691 187 110
BsrGI T'GTAC_A
Cuts at: 96 2303 2499 96
Size: 2207 196 3454
Fragments arranged by size:
3454 2207 196
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5857
BssSI C'ACGA_G
Cuts at: 3917 4929 3917
Size: 1012 4845
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2566 794
Size: 1772 4085
BstBI TT'CG_AA
Cuts at: 4661 4661
Size: 5857
BstEII G'GTnAC_C
Cuts at: 1184 2266 2344 2839 1184
Size: 1082 78 495 4202
Fragments arranged by size:
4202 1082 495 78
BstXI CCAn_nnnn'nTGG
Cuts at: 2326 2326
Size: 5857
BstYI r'GATC_y
Cuts at: 1972 2160 2596 3187 3554 3939 3956 5408 5494
Size: 188 436 591 367 385 17 1452 86
Cuts at: 5494 5506 5852 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 591 436 385 367 346 188
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5305
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5118
Fragments arranged by size:
5118 683 56
BtsI GCAGTG_nn'
Cuts at: 2934 3037 3373 4167 4187 2934
Size: 103 336 794 20 4604
Fragments arranged by size:
4604 794 336 103 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5857
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5822
DraI TTT'AAA
Cuts at: 3161 3161
Size: 5857
DraIII CAC_nnn'GTG
Cuts at: 2301 2301
Size: 5857
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2699 4864 809
Size: 507 1383 2165 1802
Fragments arranged by size:
2165 1802 1383 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2316 2328 2988
Size: 54 1425 50 275 18 486 12 660
Cuts at: 2988 3304 4193 5822 8
Size: 316 889 1629 43
Fragments arranged by size:
1629 1425 889 660 486 316 275 54
50 43 18 12
EagI C'GGCC_G
Cuts at: 1537 1812 3304 1537
Size: 275 1492 4090
Fragments arranged by size:
4090 1492 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3787 1353
Size: 2434 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4444 4818 4964 387
Size: 1064 138 2855 374 146 1280
Fragments arranged by size:
2855 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2002 2305 2854 2878 3125 3920 4517
Size: 393 370 303 549 24 247 795 597
Cuts at: 4517 5304 5572 1239
Size: 787 268 1524
Fragments arranged by size:
1524 795 787 597 549 393 370 303
268 247 24
EcoNI CCTnn'n_nnAGG
Cuts at: 2330 2825 2990 2330
Size: 495 165 5197
Fragments arranged by size:
5197 495 165
EcoO109I rG'GnC_Cy
Cuts at: 2132 2142 2335 2386 2701 2132
Size: 10 193 51 315 5288
Fragments arranged by size:
5288 315 193 51 10
EcoRI G'AATT_C
Cuts at: 3166 3544 3166
Size: 378 5479
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2032 2163 2032
Size: 131 5726
FspI TGC'GCA
Cuts at: 2981 4363 2981
Size: 1382 4475
HaeII r_GCGC'y
Cuts at: 1678 2408 5004 1678
Size: 730 2596 2531
Fragments arranged by size:
2596 2531 730
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2039 2071 4498 4530 4572
Size: 32 135 32 467 32 2427 32 42
Cuts at: 4572 4604 1373
Size: 32 2626
Fragments arranged by size:
2626 2427 467 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3201 3447 669
Size: 988 281 1263 246 3079
Fragments arranged by size:
3079 1263 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5857
HpaI GTT'AAC
Cuts at: 3447 3447
Size: 5857
KpnI G_GTAC'C
Cuts at: 3182 3182
Size: 5857
MfeI C'AATT_G
Cuts at: 3456 3456
Size: 5857
MluI A'CGCG_T
Cuts at: 5733 5733
Size: 5857
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2072 2465 4971 5155 1305
Size: 767 393 2506 184 2007
Fragments arranged by size:
2506 2007 767 393 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2318 10
Size: 54 1425 343 486 3549
Fragments arranged by size:
3549 1425 486 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3053 3815 4174 4333 518
Size: 648 1887 762 359 159 2042
Fragments arranged by size:
2042 1887 762 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2874 3273 3950 5098 5343 1606
Size: 1268 399 677 1148 245 2120
Fragments arranged by size:
2120 1268 1148 677 399 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5305
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5857
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5857
NotI GC'GGCC_GC
Cuts at: 3304 3304
Size: 5857
NspI r_CATG'y
Cuts at: 1132 1229 2686 3215 4760 1132
Size: 97 1457 529 1545 2229
Fragments arranged by size:
2229 1545 1457 529 97
PasI CC'CwG_GG
Cuts at: 1348 3084 1348
Size: 1736 4121
PciI A'CATG_T
Cuts at: 4756 4756
Size: 5857
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2887 1108
Size: 1779 4078
PfoI T'CCnGG_A
Cuts at: 2282 2790 2855 2282
Size: 508 65 5284
Fragments arranged by size:
5284 508 65
PmeI GTTT'AAAC
Cuts at: 3161 3161
Size: 5857
PmlI CAC'GTG
Cuts at: 2917 2917
Size: 5857
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3926 3958 5439 5471 3926
Size: 32 1481 32 4312
Fragments arranged by size:
4312 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2132 2335 2386 2701 2132
Size: 203 51 315 5288
Fragments arranged by size:
5288 315 203 51
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5857
PsiI TTA'TAA
Cuts at: 2070 3427 2070
Size: 1357 4500
PstI C_TGCA'G
Cuts at: 830 3209 830
Size: 2379 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5857
PvuII CAG'CTG
Cuts at: 2874 2874
Size: 5857
RsrII CG'GwC_CG
Cuts at: 2435 2435
Size: 5857
SalI G'TCGA_C
Cuts at: 1936 3199 1936
Size: 1263 4594
SanDI GG'GwC_CC
Cuts at: 2132 2386 2132
Size: 254 5603
SbfI CC_TGCA'GG
Cuts at: 3209 3209
Size: 5857
ScaI AGT'ACT
Cuts at: 4105 5817 4105
Size: 1712 4145
SexAI A'CCwGG_T
Cuts at: 2270 2270
Size: 5857
SfcI C'TryA_G
Cuts at: 826 1045 2833 3205 4340 5021 5212 826
Size: 219 1788 372 1135 681 191 1471
Fragments arranged by size:
1788 1471 1135 681 372 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5857
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5857
SmaI CCC'GGG
Cuts at: 1872 3184 1872
Size: 1312 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2137 2189 3961 4862 5124 820
Size: 197 926 194 52 1772 901 262 1553
Fragments arranged by size:
1772 1553 926 901 262 197 194 52
SnaBI TAC'GTA
Cuts at: 493 3648 493
Size: 3155 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5857
SphI G_CATG'C
Cuts at: 1132 2686 3215 1132
Size: 1554 529 3774
Fragments arranged by size:
3774 1554 529
SspI AAT'ATT
Cuts at: 5 52 2546 3781 5
Size: 47 2494 1235 2081
Fragments arranged by size:
2494 2081 1235 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5857
StyI C'CwwG_G
Cuts at: 513 1065 2525 2633 3296 513
Size: 552 1460 108 663 3074
Fragments arranged by size:
3074 1460 663 552 108
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2214 2617 3884 3901 4054 4239 4684
Size: 1255 634 403 1267 17 153 185 445
Cuts at: 4684 325
Size: 1498
Fragments arranged by size:
1498 1267 1255 634 445 403 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2303 2499 4103
Size: 274 80 33 51 1443 326 196 1604
Cuts at: 4103 5815 96
Size: 1712 138
Fragments arranged by size:
1712 1604 1443 326 274 196 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5817 5849 5817
Size: 32 5825
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5857
XbaI T'CTAG_A
Cuts at: 3193 3193
Size: 5857
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3086 3116 3086
Size: 30 5827
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5857
XmaI C'CCGG_G
Cuts at: 1870 3182 1870
Size: 1312 4545
XmnI GAAnn'nnTTC
Cuts at: 3986 3986
Size: 5857
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3665 276
Size: 53 83 186 936 2131 2468
Fragments arranged by size:
2468 2131 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PstI PvuI PvuII RsrII SalI
SanDI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BstZ17I Bsu36I EcoICRI EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PspOMI PspXI PsrI SacI SacII SapI SfiI
SfoI SrfI SwaI