Restriction map for
pFN21AB4718
(Circular) (Six-base) MAPSORT of: pFN21AB4718.seq Check: 8296 from: 1 to: 5338
pFN21AB4718 5338 bp
With 182 enzymes: SgfI *
February 27, 2009 14:37 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3148 278
Size: 53 83 186 936 1612 2468
Fragments arranged by size:
2468 1612 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2187 2681 1937
Size: 250 494 4594
Fragments arranged by size:
4594 494 250
Acc65I G'GTAC_C
Cuts at: 2586 2659 2586
Size: 73 5265
AclI AA'CG_TT
Cuts at: 1563 3465 3838 1563
Size: 1902 373 3063
Fragments arranged by size:
3063 1902 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3401 4785 5053 1632
Size: 1769 1384 268 1917
Fragments arranged by size:
1917 1769 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 2282 820
Size: 197 1265 3876
Fragments arranged by size:
3876 1265 197
AflIII A'CryG_T
Cuts at: 1203 4237 5214 1203
Size: 3034 977 1327
Fragments arranged by size:
3034 1327 977
AgeI A'CCGG_T
Cuts at: 4169 4169
Size: 5338
AhdI GACnn_n'nnGTC
Cuts at: 4067 4067
Size: 5338
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5338
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5338
AlwNI CAG_nnn'CTG
Cuts at: 2311 3081 4653 2311
Size: 770 1572 2996
Fragments arranged by size:
2996 1572 770
ApaI G_GGCC'C
Cuts at: 2172 2602 2172
Size: 430 4908
ApaLI G'TGCA_C
Cuts at: 2074 3395 4551 2074
Size: 1321 1156 2861
Fragments arranged by size:
2861 1321 1156
ApoI r'AATT_y
Cuts at: 1493 2647 2873 3025 5134 1493
Size: 1154 226 152 2109 1697
Fragments arranged by size:
2109 1697 1154 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3892 160
Size: 872 2860 1606
Fragments arranged by size:
2860 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5338
AvaI C'yCGr_G
Cuts at: 1870 1943 2018 2663 1870
Size: 73 75 645 4545
Fragments arranged by size:
4545 645 75 73
BamHI G'GATC_C
Cuts at: 2668 3035 2668
Size: 367 4971
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2063 2586 2659 4114 618
Size: 325 217 627 276 523 73 1455 1842
Fragments arranged by size:
1842 1455 627 523 325 276 217 73
BanII G_rGCy'C
Cuts at: 1391 2025 2172 2602 2657 1391
Size: 634 147 430 55 4072
Fragments arranged by size:
4072 634 430 147 55
BbeI G_GCGC'C
Cuts at: 2067 2067
Size: 5338
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4367
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3529 3563 1903
Size: 34 1592 34 3678
Fragments arranged by size:
3678 1592 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3230 4440 3230
Size: 1210 4128
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5338
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3949 136
Size: 107 122 71 631 605 2277 1525
Fragments arranged by size:
2277 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5333 5333
Size: 5338
BlpI GC'TnA_GC
Cuts at: 2755 2755
Size: 5338
Bme1580I G_kGCm'C
Cuts at: 1790 2078 2172 2602 3399 4555 1790
Size: 288 94 430 797 1156 2573
Fragments arranged by size:
2573 1156 797 430 288 94
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5338
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2068 3069 4027
Size: 416 53 83 399 24 651 1001 958
Cuts at: 4027 442
Size: 1753
Fragments arranged by size:
1753 1001 958 651 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5338
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2065 2309 3998 1239
Size: 826 244 1689 2579
Fragments arranged by size:
2579 1689 826 244
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3463 4328 4626 3463
Size: 865 298 4175
Fragments arranged by size:
4175 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4001 882
Size: 617 2502 2219
Fragments arranged by size:
2502 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3129 493
Size: 711 1925 2702
Fragments arranged by size:
2702 1925 711
BsaBI GATnn'nnATC
Cuts at: 5332 5332
Size: 5338
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2064 2103 3145 3527
Size: 53 83 186 936 531 39 1042 382
Cuts at: 3527 4162 275
Size: 635 1451
Fragments arranged by size:
1451 1042 936 635 531 382 186 83
53 39
BsaWI w'CCGG_w
Cuts at: 3771 4169 4443 4590 5001 3771
Size: 398 274 147 411 4108
Fragments arranged by size:
4108 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2495 2525 1178
Size: 30 165 30 1092 30 3991
Fragments arranged by size:
3991 1092 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5338
BseYI C'CCAG_C
Cuts at: 2087 2394 4541 5037 2087
Size: 307 2147 496 2388
Fragments arranged by size:
2388 2147 496 307
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2261 1163
Size: 573 525 4240
Fragments arranged by size:
4240 573 525
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2788 3549 4577 1540
Size: 275 186 787 761 1028 2301
Fragments arranged by size:
2301 1028 787 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2034 2078 3399 3484 4555 2034
Size: 44 1321 85 1071 2817
Fragments arranged by size:
2817 1321 1071 85 44
BsmI GAATG_Cn'
Cuts at: 2849 2942 2849
Size: 93 5245
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2025 2034 2078 2172 2602 2657 3399
Size: 399 235 9 44 94 430 55 742
Cuts at: 3399 3484 4555 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 430 399 235 94 85
55 44 9
BspEI T'CCGG_A
Cuts at: 5001 5001
Size: 5338
BspHI T'CATG_A
Cuts at: 3227 4957 3227
Size: 1730 3608
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2319 2469 2693 844
Size: 1475 150 224 3489
Fragments arranged by size:
3489 1475 224 150
BsrBI CCG'CTC
Cuts at: 1394 1823 3225 1394
Size: 429 1402 3507
Fragments arranged by size:
3507 1402 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3833 4007 65
Size: 1183 2585 174 1396
Fragments arranged by size:
2585 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3982 4169 1700
Size: 110 2172 187 2869
Fragments arranged by size:
2869 2172 187 110
BsrGI T'GTAC_A
Cuts at: 96 2343 96
Size: 2247 3091
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5338
BssSI C'ACGA_G
Cuts at: 3398 4410 3398
Size: 1012 4326
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5338
BstBI TT'CG_AA
Cuts at: 4142 4142
Size: 5338
BstEII G'GTnAC_C
Cuts at: 1184 2522 1184
Size: 1338 4000
BstXI CCAn_nnnn'nTGG
Cuts at: 2143 2143
Size: 5338
BstYI r'GATC_y
Cuts at: 1972 2668 3035 3420 3437 4889 4975 4987 5333
Size: 696 367 385 17 1452 86 12 346
Cuts at: 5333 1972
Size: 1977
Fragments arranged by size:
1977 1452 696 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4786
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4599
Fragments arranged by size:
4599 683 56
BtsI GCAGTG_nn'
Cuts at: 2854 3648 3668 2854
Size: 794 20 4524
Fragments arranged by size:
4524 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5338
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5303
DraI TTT'AAA
Cuts at: 2642 2642
Size: 5338
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4345 809
Size: 507 3029 1802
Fragments arranged by size:
3029 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2785 3674 5303
Size: 54 1425 50 275 18 955 889 1629
Cuts at: 5303 8
Size: 43
Fragments arranged by size:
1629 1425 955 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2785 1537
Size: 275 973 4090
Fragments arranged by size:
4090 973 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3268 1353
Size: 1915 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3925 4299 4445 387
Size: 1064 138 2336 374 146 1280
Fragments arranged by size:
2336 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2065 2309 3401 3998 4785 5053 1239
Size: 393 433 244 1092 597 787 268 1524
Fragments arranged by size:
1524 1092 787 597 433 393 268 244
EcoO109I rG'GnC_Cy
Cuts at: 2168 2169 2599 2168
Size: 1 430 4907
Fragments arranged by size:
4907 430 1
EcoRI G'AATT_C
Cuts at: 2647 3025 2647
Size: 378 4960
FspI TGC'GCA
Cuts at: 3844 3844
Size: 5338
HaeII r_GCGC'y
Cuts at: 1678 2067 4485 1678
Size: 389 2418 2531
Fragments arranged by size:
2531 2418 389
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2495 2527 3979 4011 4053
Size: 32 135 32 923 32 1452 32 42
Cuts at: 4053 4085 1373
Size: 32 2626
Fragments arranged by size:
2626 1452 923 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2296 2682 2928 669
Size: 988 281 358 386 246 3079
Fragments arranged by size:
3079 988 386 358 281 246
HindIII A'AGCT_T
Cuts at: 748 2259 748
Size: 1511 3827
HpaI GTT'AAC
Cuts at: 2928 2928
Size: 5338
KasI G'GCGC_C
Cuts at: 2063 2063
Size: 5338
KpnI G_GTAC'C
Cuts at: 2590 2663 2590
Size: 73 5265
MfeI C'AATT_G
Cuts at: 2937 2937
Size: 5338
MluI A'CGCG_T
Cuts at: 5214 5214
Size: 5338
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2142 4452 4636 1305
Size: 837 2310 184 2007
Fragments arranged by size:
2310 2007 837 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3516
Fragments arranged by size:
3516 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2228 3296 3655 3814 518
Size: 648 1062 1068 359 159 2042
Fragments arranged by size:
2042 1068 1062 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2043 2583 2754 3431 4579 4824 1606
Size: 437 540 171 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 540 437 245 171
NarI GG'CG_CC
Cuts at: 2064 2064
Size: 5338
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4786
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5338
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5338
NotI GC'GGCC_GC
Cuts at: 2785 2785
Size: 5338
NspI r_CATG'y
Cuts at: 1132 1229 2082 2509 2696 4241 1132
Size: 97 853 427 187 1545 2229
Fragments arranged by size:
2229 1545 853 427 187 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5338
PciI A'CATG_T
Cuts at: 4237 4237
Size: 5338
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5338
PmeI GTTT'AAAC
Cuts at: 2642 2642
Size: 5338
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3407 3439 4920 4952 3407
Size: 32 1481 32 3793
Fragments arranged by size:
3793 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5338
PsiI TTA'TAA
Cuts at: 2908 2908
Size: 5338
PspOMI G'GGCC_C
Cuts at: 2168 2598 2168
Size: 430 4908
PstI C_TGCA'G
Cuts at: 830 2582 2690 830
Size: 1752 108 3478
Fragments arranged by size:
3478 1752 108
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5338
PvuII CAG'CTG
Cuts at: 2043 2583 2043
Size: 540 4798
SalI G'TCGA_C
Cuts at: 1936 2680 1936
Size: 744 4594
SbfI CC_TGCA'GG
Cuts at: 2690 2690
Size: 5338
ScaI AGT'ACT
Cuts at: 3586 5298 3586
Size: 1712 3626
SfcI C'TryA_G
Cuts at: 826 1045 2578 2686 3821 4502 4693 826
Size: 219 1533 108 1135 681 191 1471
Fragments arranged by size:
1533 1471 1135 681 219 191 108
SfoI GGC'GCC
Cuts at: 2065 2065
Size: 5338
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5338
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5338
SmaI CCC'GGG
Cuts at: 1872 2665 1872
Size: 793 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2282 3442 4343 4605 820
Size: 197 926 339 1160 901 262 1553
Fragments arranged by size:
1553 1160 926 901 339 262 197
SnaBI TAC'GTA
Cuts at: 493 3129 493
Size: 2636 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5338
SphI G_CATG'C
Cuts at: 1132 2696 1132
Size: 1564 3774
SspI AAT'ATT
Cuts at: 5 52 3262 5
Size: 47 3210 2081
Fragments arranged by size:
3210 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5338
StyI C'CwwG_G
Cuts at: 513 1065 2012 2136 2777 513
Size: 552 947 124 641 3074
Fragments arranged by size:
3074 947 641 552 124
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2516 3365 3382 3535 3720 4165 325
Size: 1255 936 849 17 153 185 445 1498
Fragments arranged by size:
1498 1255 936 849 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2244 2343 3584
Size: 274 80 33 51 1443 267 99 1241
Cuts at: 3584 5296 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1241 274 267 138 99 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5298 5330 5298
Size: 32 5306
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5338
XbaI T'CTAG_A
Cuts at: 2674 2674
Size: 5338
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5338
XmaI C'CCGG_G
Cuts at: 1870 2663 1870
Size: 793 4545
XmnI GAAnn'nnTTC
Cuts at: 3467 3467
Size: 5338
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3146 276
Size: 53 83 186 936 1612 2468
Fragments arranged by size:
2468 1612 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NarI NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI
PstI PvuI PvuII SalI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI
NsiI PacI PfoI PmlI PpuMI PspXI PsrI RsrII
SacI SacII SanDI SapI SexAI SfiI SrfI SwaI
XcmI