Restriction map for
pFN21AB4713
(Circular) (Six-base) MAPSORT of: pFN21AB4713.seq Check: 4663 from: 1 to: 7033
pFN21AB4713 7033 bp
With 182 enzymes: SgfI *
August 16, 2010 14:42 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2243 2243
Size: 7033
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4843 278
Size: 53 83 186 936 3307 2468
Fragments arranged by size:
3307 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4376 1937
Size: 2439 4594
Acc65I G'GTAC_C
Cuts at: 4354 4354
Size: 7033
AclI AA'CG_TT
Cuts at: 1563 5160 5533 1563
Size: 3597 373 3063
Fragments arranged by size:
3597 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2369 2724 2829 2994 3687 5096 6480 6748
Size: 737 355 105 165 693 1409 1384 268
Cuts at: 6748 1632
Size: 1917
Fragments arranged by size:
1917 1409 1384 737 693 355 268 165
105
AflII C'TTAA_G
Cuts at: 820 1017 4064 820
Size: 197 3047 3789
Fragments arranged by size:
3789 3047 197
AflIII A'CryG_T
Cuts at: 1203 5932 6909 1203
Size: 4729 977 1327
Fragments arranged by size:
4729 1327 977
AgeI A'CCGG_T
Cuts at: 5864 5864
Size: 7033
AhdI GACnn_n'nnGTC
Cuts at: 5762 5762
Size: 7033
AleI CACnn'nnGTG
Cuts at: 1166 2246 2534 1166
Size: 1080 288 5665
Fragments arranged by size:
5665 1080 288
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7033
AlwNI CAG_nnn'CTG
Cuts at: 2735 4099 4776 6348 2735
Size: 1364 677 1572 3420
Fragments arranged by size:
3420 1572 1364 677
ApaI G_GGCC'C
Cuts at: 2170 2204 3713 2170
Size: 34 1509 5490
Fragments arranged by size:
5490 1509 34
ApaLI G'TGCA_C
Cuts at: 5090 6246 5090
Size: 1156 5877
ApoI r'AATT_y
Cuts at: 1493 2379 4115 4342 4568 4720 6829 1493
Size: 886 1736 227 226 152 2109 1697
Fragments arranged by size:
2109 1736 1697 886 227 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5587 160
Size: 872 4555 1606
Fragments arranged by size:
4555 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7033
AvaI C'yCGr_G
Cuts at: 1870 1943 2054 2149 2169 2197 2609 2642 3155
Size: 73 111 95 20 28 412 33 513
Cuts at: 3155 3605 3676 4358 1870
Size: 450 71 682 4545
Fragments arranged by size:
4545 682 513 450 412 111 95 73
71 33 28 20
BamHI G'GATC_C
Cuts at: 2020 4363 4730 2020
Size: 2343 367 4323
Fragments arranged by size:
4323 2343 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2073 2125 2159 3731 4354
Size: 325 217 627 286 52 34 1572 623
Cuts at: 4354 5809 618
Size: 1455 1842
Fragments arranged by size:
1842 1572 1455 627 623 325 286 217
52 34
BanII G_rGCy'C
Cuts at: 1391 2145 2170 2204 2423 2827 3402 3446 3594
Size: 754 25 34 219 404 575 44 148
Cuts at: 3594 3631 3713 4352 1391
Size: 37 82 639 4072
Fragments arranged by size:
4072 754 639 575 404 219 148 82
44 37 34 25
BbeI G_GCGC'C
Cuts at: 2129 2163 3735 2129
Size: 34 1572 5427
Fragments arranged by size:
5427 1572 34
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2906 2982 3678 928
Size: 971 1007 76 696 4283
Fragments arranged by size:
4283 1007 971 696 76
BbvCI CC'TCA_GC
Cuts at: 4329 4329
Size: 7033
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3512 3546 5224 5258 1903
Size: 34 1575 34 1678 34 3678
Fragments arranged by size:
3678 1678 1575 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4925 6135 4925
Size: 1210 5823
BclI T'GATC_A
Cuts at: 1548 3072 1548
Size: 1524 5509
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2123 3315 3428
Size: 107 122 71 631 605 451 1192 113
Cuts at: 3428 3541 3546 3884 5644 136
Size: 113 5 338 1760 1525
Fragments arranged by size:
1760 1525 1192 631 605 451 338 122
113 113 107 71 5
BglII A'GATC_T
Cuts at: 2544 7028 2544
Size: 4484 2549
BlpI GC'TnA_GC
Cuts at: 4450 4450
Size: 7033
Bme1580I G_kGCm'C
Cuts at: 1790 2170 2204 3713 5094 6250 1790
Size: 380 34 1509 1381 1156 2573
Fragments arranged by size:
2573 1509 1381 1156 380 34
BmgBI CAC'GTC
Cuts at: 1318 2404 1318
Size: 1086 5947
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2056 3847 4764
Size: 416 53 83 399 24 639 1791 917
Cuts at: 4764 5722 442
Size: 958 1753
Fragments arranged by size:
1791 1753 958 917 639 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7033
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3478 3478
Size: 7033
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3639 3653 5693 1239
Size: 2400 14 2040 2579
Fragments arranged by size:
2579 2400 2040 14
Bpu10I CC'TnA_GC
Cuts at: 2069 4329 2069
Size: 2260 4773
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4013 5158 6023 6321 4013
Size: 1145 865 298 4725
Fragments arranged by size:
4725 1145 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5696 882
Size: 617 4197 2219
Fragments arranged by size:
4197 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3313 4051 4824 493
Size: 711 2109 738 773 2702
Fragments arranged by size:
2702 2109 773 738 711
BsaBI GATnn'nnATC
Cuts at: 7027 7027
Size: 7033
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2105 2126 2160 2180
Size: 53 83 186 936 572 21 34 20
Cuts at: 2180 3420 3513 3644 3732 4840 5222 5857 275
Size: 1240 93 131 88 1108 382 635 1451
Fragments arranged by size:
1451 1240 1108 936 635 572 382 186
131 93 88 83 53 34 21 20
BsaWI w'CCGG_w
Cuts at: 3173 5466 5864 6138 6285 6696 3173
Size: 2293 398 274 147 411 3510
Fragments arranged by size:
3510 2293 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2770 2800 1178
Size: 30 165 30 1367 30 5411
Fragments arranged by size:
5411 1367 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2441 2627 2817 3386 3415 3452 3518 3539
Size: 1255 186 190 569 29 37 66 21
Cuts at: 3539 4090 1186
Size: 551 4129
Fragments arranged by size:
4129 1255 569 551 190 186 66 37
29 21
BseYI C'CCAG_C
Cuts at: 2135 2144 2431 3191 3479 3594 6236 6732 2135
Size: 9 287 760 288 115 2642 496 2436
Fragments arranged by size:
2642 2436 760 496 288 287 115 9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3968 1163
Size: 573 2232 4228
Fragments arranged by size:
4228 2232 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3131 4483 5244 6272 1540
Size: 275 186 1130 1352 761 1028 2301
Fragments arranged by size:
2301 1352 1130 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3402 5094 5179 6250 3402
Size: 1692 85 1071 4185
Fragments arranged by size:
4185 1692 1071 85
BsmI GAATG_Cn'
Cuts at: 4544 4637 4544
Size: 93 6940
BsmBI CGTCTCn'nnnn_
Cuts at: 3615 3635 3615
Size: 20 7013
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2145 2170 2204 2423 2827 3402 3446
Size: 399 355 25 34 219 404 575 44
Cuts at: 3446 3594 3631 3713 4352 5094 5179 6250 1391
Size: 148 37 82 639 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 639 575 404 399 355
219 148 85 82 44 37 34 25
BspEI T'CCGG_A
Cuts at: 3173 6696 3173
Size: 3523 3510
BspHI T'CATG_A
Cuts at: 4922 6652 4922
Size: 1730 5303
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2243 4388 844
Size: 1399 2145 3489
Fragments arranged by size:
3489 2145 1399
BsrBI CCG'CTC
Cuts at: 1394 1823 4920 1394
Size: 429 3097 3507
Fragments arranged by size:
3507 3097 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5528 5702 65
Size: 1183 4280 174 1396
Fragments arranged by size:
4280 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2048 2122 2192 3130 3540 5677 5864
Size: 110 238 74 70 938 410 2137 187
Cuts at: 5864 1700
Size: 2869
Fragments arranged by size:
2869 2137 938 410 238 187 110 74
70
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7033
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7033
BssSI C'ACGA_G
Cuts at: 2478 3206 5093 6105 2478
Size: 728 1887 1012 3406
Fragments arranged by size:
3406 1887 1012 728
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7033
BstBI TT'CG_AA
Cuts at: 5837 5837
Size: 7033
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7033
BstXI CCAn_nnnn'nTGG
Cuts at: 2248 2269 3199 3487 2248
Size: 21 930 288 5794
Fragments arranged by size:
5794 930 288 21
BstYI r'GATC_y
Cuts at: 1972 2020 2544 2990 4363 4730 5115 5132 6584
Size: 48 524 446 1373 367 385 17 1452
Cuts at: 6584 6670 6682 7028 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1373 524 446 385 367 346
86 48 17 12
Bsu36I CC'TnA_GG
Cuts at: 2352 2424 3289 3365 2352
Size: 72 865 76 6020
Fragments arranged by size:
6020 865 76 72
BtgI C'CryG_G
Cuts at: 513 1065 2004 2226 2268 2529 3125 3264 3270
Size: 552 939 222 42 261 596 139 6
Cuts at: 3270 3656 513
Size: 386 3890
Fragments arranged by size:
3890 939 596 552 386 261 222 139
42 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6294
Fragments arranged by size:
6294 683 56
BtsI GCAGTG_nn'
Cuts at: 3743 3951 4549 5343 5363 3743
Size: 208 598 794 20 5413
Fragments arranged by size:
5413 794 598 208 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7033
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6998
DraI TTT'AAA
Cuts at: 4337 4337
Size: 7033
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6040 809
Size: 507 4724 1802
Fragments arranged by size:
4724 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2046 2398
Size: 54 1425 50 275 18 177 39 352
Cuts at: 2398 3128 3144 3267 3307 3552 3699 3891 4480
Size: 730 16 123 40 245 147 192 589
Cuts at: 4480 5369 6998 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 730 589 352 275 245
192 177 147 123 54 50 43 40
39 18 16
EagI C'GGCC_G
Cuts at: 1537 1812 3128 4480 1537
Size: 275 1316 1352 4090
Fragments arranged by size:
4090 1352 1316 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3845 4088 4163 4963 1353
Size: 2492 243 75 800 3423
Fragments arranged by size:
3423 2492 800 243 75
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5620 5994 6140 387
Size: 1064 138 4031 374 146 1280
Fragments arranged by size:
4031 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3400 3400
Size: 7033
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2369 2724 2829 2994 3639 3653 3687
Size: 393 737 355 105 165 645 14 34
Cuts at: 3687 5096 5693 6480 6748 1239
Size: 1409 597 787 268 1524
Fragments arranged by size:
1524 1409 787 737 645 597 393 355
268 165 105 34 14
EcoNI CCTnn'n_nnAGG
Cuts at: 3368 4007 4091 3368
Size: 639 84 6310
Fragments arranged by size:
6310 639 84
EcoO109I rG'GnC_Cy
Cuts at: 2058 2166 2201 3259 3547 3709 3710 3725 4096
Size: 108 35 1058 288 162 1 15 371
Cuts at: 4096 2058
Size: 4995
Fragments arranged by size:
4995 1058 371 288 162 108 35 15
1
EcoRI G'AATT_C
Cuts at: 2379 4342 4720 2379
Size: 1963 378 4692
Fragments arranged by size:
4692 1963 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4000 4000
Size: 7033
FseI GG_CCGG'CC
Cuts at: 2052 2052
Size: 7033
FspI TGC'GCA
Cuts at: 5539 5539
Size: 7033
HaeII r_GCGC'y
Cuts at: 1678 2129 2163 3486 3735 4155 4317 6180 1678
Size: 451 34 1323 249 420 162 1863 2531
Fragments arranged by size:
2531 1863 1323 451 420 249 162 34
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2461 2493 2789 2821 3236
Size: 32 135 32 889 32 296 32 415
Cuts at: 3236 3268 3410 3442 3566 3598 5674 5706 5748
Size: 32 142 32 124 32 2076 32 42
Cuts at: 5748 5780 1373
Size: 32 2626
Fragments arranged by size:
2626 2076 889 415 296 142 135 124
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4377 4623 669
Size: 988 281 2439 246 3079
Fragments arranged by size:
3079 2439 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7033
HpaI GTT'AAC
Cuts at: 4623 4623
Size: 7033
KasI G'GCGC_C
Cuts at: 2125 2159 3731 2125
Size: 34 1572 5427
Fragments arranged by size:
5427 1572 34
KpnI G_GTAC'C
Cuts at: 4358 4358
Size: 7033
MfeI C'AATT_G
Cuts at: 4632 4632
Size: 7033
MluI A'CGCG_T
Cuts at: 6909 6909
Size: 7033
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3708 6147 6331 1305
Size: 2403 2439 184 2007
Fragments arranged by size:
2439 2403 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2400 3309 10
Size: 54 1425 343 568 909 3734
Fragments arranged by size:
3734 1425 909 568 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2246 2534 4187 4275 4991 5350 5509
Size: 648 1080 288 1653 88 716 359 159
Cuts at: 5509 518
Size: 2042
Fragments arranged by size:
2042 1653 1080 716 648 359 288 159
88
MspA1I CmG'CkG
Cuts at: 1606 2044 3142 3272 4449 5126 6274 6519 1606
Size: 438 1098 130 1177 677 1148 245 2120
Fragments arranged by size:
2120 1177 1148 1098 677 438 245 130
NaeI GCC'GGC
Cuts at: 2050 2124 2194 3542 2050
Size: 74 70 1348 5541
Fragments arranged by size:
5541 1348 74 70
NarI GG'CG_CC
Cuts at: 2126 2160 3732 2126
Size: 34 1572 5427
Fragments arranged by size:
5427 1572 34
NcoI C'CATG_G
Cuts at: 513 1065 2004 2226 2268 3264 513
Size: 552 939 222 42 996 4282
Fragments arranged by size:
4282 996 939 552 222 42
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7033
NgoMIV G'CCGG_C
Cuts at: 2048 2122 2192 3540 2048
Size: 74 70 1348 5541
Fragments arranged by size:
5541 1348 74 70
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7033
NotI GC'GGCC_GC
Cuts at: 4480 4480
Size: 7033
NspI r_CATG'y
Cuts at: 1132 1229 4391 5936 1132
Size: 97 3162 1545 2229
Fragments arranged by size:
3162 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 3040 3713 1348
Size: 1692 673 4668
Fragments arranged by size:
4668 1692 673
PciI A'CATG_T
Cuts at: 5932 5932
Size: 7033
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2767 3011 4099 4252 1108
Size: 1659 244 1088 153 3889
Fragments arranged by size:
3889 1659 1088 244 153
PfoI T'CCnGG_A
Cuts at: 3498 4092 3498
Size: 594 6439
PmeI GTTT'AAAC
Cuts at: 4337 4337
Size: 7033
PmlI CAC'GTG
Cuts at: 3313 3313
Size: 7033
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5102 5134 6615 6647 5102
Size: 32 1481 32 5488
Fragments arranged by size:
5488 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2058 3259 3725 4096 2058
Size: 1201 466 371 4995
Fragments arranged by size:
4995 1201 466 371
PshAI GACnn'nnGTC
Cuts at: 1613 3512 1613
Size: 1899 5134
PsiI TTA'TAA
Cuts at: 4603 4603
Size: 7033
PspOMI G'GGCC_C
Cuts at: 2166 2200 3709 2166
Size: 34 1509 5490
Fragments arranged by size:
5490 1509 34
PspXI vC'TCGA_Gb
Cuts at: 3676 3676
Size: 7033
PstI C_TGCA'G
Cuts at: 830 2524 3034 4044 4385 830
Size: 1694 510 1010 341 3478
Fragments arranged by size:
3478 1694 1010 510 341
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7033
PvuII CAG'CTG
Cuts at: 3142 3142
Size: 7033
RsrII CG'GwC_CG
Cuts at: 3176 3176
Size: 7033
SacI G_AGCT'C
Cuts at: 3402 3402
Size: 7033
SacII CC_GC'GG
Cuts at: 3273 3273
Size: 7033
SalI G'TCGA_C
Cuts at: 1936 4375 1936
Size: 2439 4594
SanDI GG'GwC_CC
Cuts at: 2058 2058
Size: 7033
SapI GCTCTTCn'nnn_
Cuts at: 3845 4088 3845
Size: 243 6790
SbfI CC_TGCA'GG
Cuts at: 4385 4385
Size: 7033
ScaI AGT'ACT
Cuts at: 5281 6993 5281
Size: 1712 5321
SexAI A'CCwGG_T
Cuts at: 4098 4098
Size: 7033
SfcI C'TryA_G
Cuts at: 826 1045 2520 3030 4040 4212 4381 5516 6197
Size: 219 1475 510 1010 172 169 1135 681
Cuts at: 6197 6388 826
Size: 191 1471
Fragments arranged by size:
1475 1471 1135 1010 681 510 219 191
172 169
SfiI GGCCn_nnn'nGGCC
Cuts at: 3884 3884
Size: 7033
SfoI GGC'GCC
Cuts at: 2127 2161 3733 2127
Size: 34 1572 5427
Fragments arranged by size:
5427 1572 34
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7033
SgrAI Cr'CCGG_yG
Cuts at: 1700 2122 1700
Size: 422 6611
SmaI CCC'GGG
Cuts at: 1872 2056 2151 2171 4360 1872
Size: 184 95 20 2189 4545
Fragments arranged by size:
4545 2189 184 95 20
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3676 3992 4064 5137 6038 6300
Size: 197 926 1733 316 72 1073 901 262
Cuts at: 6300 820
Size: 1553
Fragments arranged by size:
1733 1553 1073 926 901 316 262 197
72
SnaBI TAC'GTA
Cuts at: 493 4824 493
Size: 4331 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7033
SphI G_CATG'C
Cuts at: 1132 4391 1132
Size: 3259 3774
SspI AAT'ATT
Cuts at: 5 52 4957 5
Size: 47 4905 2081
Fragments arranged by size:
4905 2081 47
StuI AGG'CCT
Cuts at: 1517 2582 3980 1517
Size: 1065 1398 4570
Fragments arranged by size:
4570 1398 1065
StyI C'CwwG_G
Cuts at: 513 1065 2004 2226 2268 3264 4472 513
Size: 552 939 222 42 996 1208 3074
Fragments arranged by size:
3074 1208 996 939 552 222 42
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5060 5077 5230 5415 5860 325
Size: 1255 3480 17 153 185 445 1498
Fragments arranged by size:
3480 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3354 4026 5279
Size: 274 80 33 51 1443 1377 672 1253
Cuts at: 5279 6991 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1377 1253 672 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2297 2329 2770 2802 6993 7025 2297
Size: 32 441 32 4191 32 2305
Fragments arranged by size:
4191 2305 441 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2945 1316
Size: 1629 5404
XbaI T'CTAG_A
Cuts at: 4369 4369
Size: 7033
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2152 2152
Size: 7033
XhoI C'TCGA_G
Cuts at: 1943 3676 1943
Size: 1733 5300
XmaI C'CCGG_G
Cuts at: 1870 2054 2149 2169 4358 1870
Size: 184 95 20 2189 4545
Fragments arranged by size:
4545 2189 184 95 20
XmnI GAAnn'nnTTC
Cuts at: 5162 5162
Size: 7033
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4841 276
Size: 53 83 186 936 3307 2468
Fragments arranged by size:
3307 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI
PstI PvuI PvuII RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BfrBI BsiWI BstZ17I
DraIII EcoRV FspAI NruI NsiI PacI PsrI SrfI
SwaI