Restriction map for
pFN21AB4700
(Circular) (Six-base) MAPSORT of: pFN21AB4700.seq Check: 9614 from: 1 to: 7423
pFN21AB4700 7423 bp
With 182 enzymes: SgfI *
February 27, 2009 14:33 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5233 278
Size: 53 83 186 936 3697 2468
Fragments arranged by size:
3697 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3038 3192 4766 1937
Size: 1101 154 1574 4594
Fragments arranged by size:
4594 1574 1101 154
Acc65I G'GTAC_C
Cuts at: 3542 4744 3542
Size: 1202 6221
AclI AA'CG_TT
Cuts at: 1563 5550 5923 1563
Size: 3987 373 3063
Fragments arranged by size:
3987 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2045 2136 2393 5486 6870 7138 1632
Size: 413 91 257 3093 1384 268 1917
Fragments arranged by size:
3093 1917 1384 413 268 257 91
AfeI AGC'GCT
Cuts at: 2025 2025
Size: 7423
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7226
AflIII A'CryG_T
Cuts at: 1203 2961 4701 6322 7299 1203
Size: 1758 1740 1621 977 1327
Fragments arranged by size:
1758 1740 1621 1327 977
AgeI A'CCGG_T
Cuts at: 4169 6254 4169
Size: 2085 5338
AhdI GACnn_n'nnGTC
Cuts at: 6152 6152
Size: 7423
AleI CACnn'nnGTG
Cuts at: 1166 4136 1166
Size: 2970 4453
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3856 1184
Size: 2672 4751
AlwNI CAG_nnn'CTG
Cuts at: 2609 2834 5166 6738 2609
Size: 225 2332 1572 3294
Fragments arranged by size:
3294 2332 1572 225
ApaLI G'TGCA_C
Cuts at: 5480 6636 5480
Size: 1156 6267
ApoI r'AATT_y
Cuts at: 1493 3423 3747 4010 4732 4958 5110 7219 1493
Size: 1930 324 263 722 226 152 2109 1697
Fragments arranged by size:
2109 1930 1697 722 324 263 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2730 3798 3867 4512 5977 160
Size: 872 1698 1068 69 645 1465 1606
Fragments arranged by size:
1698 1606 1465 1068 872 645 69
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7423
AvaI C'yCGr_G
Cuts at: 1870 1943 2650 4748 1870
Size: 73 707 2098 4545
Fragments arranged by size:
4545 2098 707 73
BamHI G'GATC_C
Cuts at: 4753 5120 4753
Size: 367 7056
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3542 4166 4172 4744 6199
Size: 325 217 627 1755 624 6 572 1455
Cuts at: 6199 618
Size: 1842
Fragments arranged by size:
1842 1755 1455 627 624 572 325 217
6
BanII G_rGCy'C
Cuts at: 1391 4458 4619 4742 1391
Size: 3067 161 123 4072
Fragments arranged by size:
4072 3067 161 123
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 4353 928
Size: 971 2454 3998
Fragments arranged by size:
3998 2454 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2121 2131 2155 2165 2879 2913 5614
Size: 34 184 10 24 10 714 34 2701
Cuts at: 5614 5648 1903
Size: 34 3678
Fragments arranged by size:
3678 2701 714 184 34 34 34 24
10 10
BciVI GTATCCnnnnn_n'
Cuts at: 4473 5315 6525 4473
Size: 842 1210 5371
Fragments arranged by size:
5371 1210 842
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7423
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6034 136
Size: 107 122 71 631 605 4362 1525
Fragments arranged by size:
4362 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3780 7418 3780
Size: 3638 3785
BlpI GC'TnA_GC
Cuts at: 3990 4840 3990
Size: 850 6573
Bme1580I G_kGCm'C
Cuts at: 1790 2716 5484 6640 1790
Size: 926 2768 1156 2573
Fragments arranged by size:
2768 2573 1156 926
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7423
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2494 2607 4453
Size: 416 53 83 399 24 1077 113 1846
Cuts at: 4453 4681 5154 6112 442
Size: 228 473 958 1753
Fragments arranged by size:
1846 1753 1077 958 473 416 399 228
113 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7423
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2503 3126 3834 3921 4512 6083 1239
Size: 1264 623 708 87 591 1571 2579
Fragments arranged by size:
2579 1571 1264 708 623 591 87
Bpu10I CC'TnA_GC
Cuts at: 3567 3567
Size: 7423
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2520 3798 3810 4384 4615 4633 5548 6413 6711
Size: 1278 12 574 231 18 915 865 298
Cuts at: 6711 2520
Size: 3232
Fragments arranged by size:
3232 1278 915 865 574 298 231 18
12
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2327 6086 882
Size: 617 828 3759 2219
Fragments arranged by size:
3759 2219 828 617
BsaAI yAC'GTr
Cuts at: 493 1204 5214 493
Size: 711 4010 2702
Fragments arranged by size:
4010 2702 711
BsaBI GATnn'nnATC
Cuts at: 3228 7417 3228
Size: 4189 3234
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2066 5230 5612 6247
Size: 53 83 186 936 533 3164 382 635
Cuts at: 6247 275
Size: 1451
Fragments arranged by size:
3164 1451 936 635 533 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4169 4465 5856 6254 6528 6675 7086 4169
Size: 296 1391 398 274 147 411 4506
Fragments arranged by size:
4506 1391 411 398 296 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 7198
Fragments arranged by size:
7198 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2405 4353 1186
Size: 1219 1948 4256
Fragments arranged by size:
4256 1948 1219
BseYI C'CCAG_C
Cuts at: 4604 6626 7122 4604
Size: 2022 496 4905
Fragments arranged by size:
4905 2022 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2151 4114 1163
Size: 573 415 1963 4472
Fragments arranged by size:
4472 1963 573 415
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3108 4873 5634 6662 1540
Size: 275 186 1107 1765 761 1028 2301
Fragments arranged by size:
2301 1765 1107 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4619 5484 5569 6640 4619
Size: 865 85 1071 5402
Fragments arranged by size:
5402 1071 865 85
BsmI GAATG_Cn'
Cuts at: 4934 5027 4934
Size: 93 7330
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2716 4458 4619 4742 5484 5569 6640
Size: 399 926 1742 161 123 742 85 1071
Cuts at: 6640 1391
Size: 2174
Fragments arranged by size:
2174 1742 1071 926 742 399 161 123
85
BspEI T'CCGG_A
Cuts at: 7086 7086
Size: 7423
BspHI T'CATG_A
Cuts at: 5312 7042 5312
Size: 1730 5693
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4778 844
Size: 3934 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 5310 1394
Size: 429 3487 3507
Fragments arranged by size:
3507 3487 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2200 4179 5918 6092 65
Size: 1183 952 1979 1739 174 1396
Fragments arranged by size:
1979 1739 1396 1183 952 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3539 4169 6067 6254 1700
Size: 110 1729 630 1898 187 2869
Fragments arranged by size:
2869 1898 1729 630 187 110
BsrGI T'GTAC_A
Cuts at: 96 3261 96
Size: 3165 4258
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7423
BssSI C'ACGA_G
Cuts at: 5483 6495 5483
Size: 1012 6411
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2882 794
Size: 2088 5335
BstBI TT'CG_AA
Cuts at: 6227 6227
Size: 7423
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7423
BstYI r'GATC_y
Cuts at: 1972 3280 3643 3780 4753 5120 5505 5522 6974
Size: 1308 363 137 973 367 385 17 1452
Cuts at: 6974 7060 7072 7418 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1308 973 385 367 363 346
137 86 17 12
BstZ17I GTA'TAC
Cuts at: 3039 3039
Size: 7423
Bsu36I CC'TnA_GG
Cuts at: 2835 2835
Size: 7423
BtgI C'CryG_G
Cuts at: 513 1065 3102 3339 4679 513
Size: 552 2037 237 1340 3257
Fragments arranged by size:
3257 2037 1340 552 237
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6684
Fragments arranged by size:
6684 683 56
BtsI GCAGTG_nn'
Cuts at: 2230 2579 3264 3561 4597 4599 4939 5733 5753
Size: 349 685 297 1036 2 340 794 20
Cuts at: 5753 2230
Size: 3900
Fragments arranged by size:
3900 1036 794 685 349 340 297 20
2
ClaI AT'CG_AT
Cuts at: 1554 3580 1554
Size: 2026 5397
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7388
DraI TTT'AAA
Cuts at: 4002 4317 4727 4002
Size: 315 410 6698
Fragments arranged by size:
6698 410 315
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3332 6430 809
Size: 507 2016 3098 1802
Fragments arranged by size:
3098 2016 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2458 3105 4870
Size: 54 1425 50 275 18 628 647 1765
Cuts at: 4870 5759 7388 8
Size: 889 1629 43
Fragments arranged by size:
1765 1629 1425 889 647 628 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3105 4870 1537
Size: 275 1293 1765 4090
Fragments arranged by size:
4090 1765 1293 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2172 2296 2471 5353 1353
Size: 819 124 175 2882 3423
Fragments arranged by size:
3423 2882 819 175 124
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3291 6010 6384 6530 387
Size: 1064 138 1702 2719 374 146 1280
Fragments arranged by size:
2719 1702 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 4617 4617
Size: 7423
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2045 2136 2393 2503 3126 3834 3921
Size: 393 413 91 257 110 623 708 87
Cuts at: 3921 4512 5486 6083 6870 7138 1239
Size: 591 974 597 787 268 1524
Fragments arranged by size:
1524 974 787 708 623 597 591 413
393 268 257 110 91 87
EcoNI CCTnn'n_nnAGG
Cuts at: 2060 2060
Size: 7423
EcoO109I rG'GnC_Cy
Cuts at: 2525 3571 4519 4608 2525
Size: 1046 948 89 5340
Fragments arranged by size:
5340 1046 948 89
EcoRI G'AATT_C
Cuts at: 3423 3747 4732 5110 3423
Size: 324 985 378 5736
Fragments arranged by size:
5736 985 378 324
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2398 2967 3548 2398
Size: 569 581 6273
Fragments arranged by size:
6273 581 569
FspI TGC'GCA
Cuts at: 5929 5929
Size: 7423
HaeII r_GCGC'y
Cuts at: 1678 2027 6570 1678
Size: 349 4543 2531
Fragments arranged by size:
4543 2531 349
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2472 2504 3674 3706 3952
Size: 32 135 32 900 32 1170 32 246
Cuts at: 3952 3984 6064 6096 6138 6170 1373
Size: 32 2080 32 42 32 2626
Fragments arranged by size:
2626 2080 1170 900 246 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3444 3552 4767 5013 669
Size: 988 281 1506 108 1215 246 3079
Fragments arranged by size:
3079 1506 1215 988 281 246 108
HindIII A'AGCT_T
Cuts at: 748 2278 748
Size: 1530 5893
HpaI GTT'AAC
Cuts at: 5013 5013
Size: 7423
KpnI G_GTAC'C
Cuts at: 3546 4748 3546
Size: 1202 6221
MfeI C'AATT_G
Cuts at: 2877 3628 3669 5022 2877
Size: 751 41 1353 5278
Fragments arranged by size:
5278 1353 751 41
MluI A'CGCG_T
Cuts at: 7299 7299
Size: 7423
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2645 4143 4447 6537 6721 1305
Size: 1340 1498 304 2090 184 2007
Fragments arranged by size:
2090 2007 1498 1340 304 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2460 10
Size: 54 1425 343 628 4973
Fragments arranged by size:
4973 1425 628 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2999 3116 4136 5381 5740 5899 518
Size: 648 1833 117 1020 1245 359 159 2042
Fragments arranged by size:
2042 1833 1245 1020 648 359 159 117
MspA1I CmG'CkG
Cuts at: 1606 2044 2659 3008 4372 4839 5516 6664 6909
Size: 438 615 349 1364 467 677 1148 245
Cuts at: 6909 1606
Size: 2120
Fragments arranged by size:
2120 1364 1148 677 615 467 438 349
245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6871
NdeI CA'TA_TG
Cuts at: 387 2857 3082 4558 387
Size: 2470 225 1476 3252
Fragments arranged by size:
3252 2470 1476 225
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7423
NotI GC'GGCC_GC
Cuts at: 4870 4870
Size: 7423
NruI TCG'CGA
Cuts at: 2685 2685
Size: 7423
NspI r_CATG'y
Cuts at: 1132 1229 2303 2720 2965 4705 4781 6326 1132
Size: 97 1074 417 245 1740 76 1545 2229
Fragments arranged by size:
2229 1740 1545 1074 417 245 97 76
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7423
PciI A'CATG_T
Cuts at: 2961 4701 6322 2961
Size: 1740 1621 4062
Fragments arranged by size:
4062 1740 1621
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3905 4490 1108
Size: 2797 585 4041
Fragments arranged by size:
4041 2797 585
PmeI GTTT'AAAC
Cuts at: 4727 4727
Size: 7423
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5492 5524 7005 7037 5492
Size: 32 1481 32 5878
Fragments arranged by size:
5878 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2525 3571 4608 2525
Size: 1046 1037 5340
Fragments arranged by size:
5340 1046 1037
PshAI GACnn'nnGTC
Cuts at: 1613 3125 1613
Size: 1512 5911
PsiI TTA'TAA
Cuts at: 4993 4993
Size: 7423
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3245 3277 3245
Size: 32 7391
PstI C_TGCA'G
Cuts at: 830 2236 3397 3780 4377 4480 4775 830
Size: 1406 1161 383 597 103 295 3478
Fragments arranged by size:
3478 1406 1161 597 383 295 103
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7423
PvuII CAG'CTG
Cuts at: 2659 3008 4372 2659
Size: 349 1364 5710
Fragments arranged by size:
5710 1364 349
RsrII CG'GwC_CG
Cuts at: 4468 4468
Size: 7423
SacI G_AGCT'C
Cuts at: 4619 4619
Size: 7423
SalI G'TCGA_C
Cuts at: 1936 4765 1936
Size: 2829 4594
SbfI CC_TGCA'GG
Cuts at: 4775 4775
Size: 7423
ScaI AGT'ACT
Cuts at: 5671 7383 5671
Size: 1712 5711
SexAI A'CCwGG_T
Cuts at: 2811 2811
Size: 7423
SfcI C'TryA_G
Cuts at: 826 1045 2070 2232 3057 3393 3776 4373 4476
Size: 219 1025 162 825 336 383 597 103
Cuts at: 4476 4771 5906 6587 6778 826
Size: 295 1135 681 191 1471
Fragments arranged by size:
1471 1135 1025 825 681 597 383 336
295 219 191 162 103
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7423
SgrAI Cr'CCGG_yG
Cuts at: 1700 4169 1700
Size: 2469 4954
SmaI CCC'GGG
Cuts at: 1872 4750 1872
Size: 2878 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2535 3813 3825 4363 4612 4630
Size: 197 926 592 1278 12 538 249 18
Cuts at: 4630 5527 6428 6690 820
Size: 897 901 262 1553
Fragments arranged by size:
1553 1278 926 901 897 592 538 262
249 197 18 12
SnaBI TAC'GTA
Cuts at: 493 5214 493
Size: 4721 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7423
SphI G_CATG'C
Cuts at: 1132 2303 4781 1132
Size: 1171 2478 3774
Fragments arranged by size:
3774 2478 1171
SspI AAT'ATT
Cuts at: 5 52 3598 3871 4081 4312 5347 5
Size: 47 3546 273 210 231 1035 2081
Fragments arranged by size:
3546 2081 1035 273 231 210 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7423
StyI C'CwwG_G
Cuts at: 513 1065 3417 4862 513
Size: 552 2352 1445 3074
Fragments arranged by size:
3074 2352 1445 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2971 3236 3323 3699 4142 5450 5467
Size: 1255 1391 265 87 376 443 1308 17
Cuts at: 5467 5620 5805 6250 325
Size: 153 185 445 1498
Fragments arranged by size:
1498 1391 1308 1255 445 443 376 265
185 153 87 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2209 3261 5669
Size: 274 80 33 51 1443 232 1052 2408
Cuts at: 5669 7381 96
Size: 1712 138
Fragments arranged by size:
2408 1712 1443 1052 274 232 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2727 2759 2844 2876 4138 4170 7383 7415 2727
Size: 32 85 32 1262 32 3213 32 2735
Fragments arranged by size:
3213 2735 1262 85 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2486 2762 1316
Size: 1170 276 5977
Fragments arranged by size:
5977 1170 276
XbaI T'CTAG_A
Cuts at: 4759 4759
Size: 7423
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2621 2819 4411 2621
Size: 198 1592 5633
Fragments arranged by size:
5633 1592 198
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7423
XmaI C'CCGG_G
Cuts at: 1870 4748 1870
Size: 2878 4545
XmnI GAAnn'nnTTC
Cuts at: 3531 5552 3531
Size: 2021 5402
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5231 276
Size: 53 83 186 936 3697 2468
Fragments arranged by size:
3697 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NruI NspI PasI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PsrI PstI PvuI PvuII RsrII
SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BsiWI BsmBI BstXI DraIII EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NsiI PacI PfoI
PmlI PspOMI PspXI SacII SanDI SapI SfiI SfoI
SrfI SwaI