Restriction map for
pFN21AB4699
(Circular) (Six-base) MAPSORT of: pFN21AB4699.seq Check: 764 from: 1 to: 5815
pFN21AB4699 5815 bp
With 182 enzymes: SgfI *
February 27, 2009 14:33 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3625 278
Size: 53 83 186 936 2089 2468
Fragments arranged by size:
2468 2089 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2607 3158 1937
Size: 670 551 4594
Fragments arranged by size:
4594 670 551
Acc65I G'GTAC_C
Cuts at: 3136 3136
Size: 5815
AclI AA'CG_TT
Cuts at: 1563 3942 4315 1563
Size: 2379 373 3063
Fragments arranged by size:
3063 2379 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2524 3878 5262 5530 1632
Size: 892 1354 1384 268 1917
Fragments arranged by size:
1917 1384 1354 892 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5618
AflIII A'CryG_T
Cuts at: 1203 2414 4714 5691 1203
Size: 1211 2300 977 1327
Fragments arranged by size:
2300 1327 1211 977
AgeI A'CCGG_T
Cuts at: 4646 4646
Size: 5815
AhdI GACnn_n'nnGTC
Cuts at: 4544 4544
Size: 5815
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5815
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5815
AlwNI CAG_nnn'CTG
Cuts at: 2975 3558 5130 2975
Size: 583 1572 3660
Fragments arranged by size:
3660 1572 583
ApaI G_GGCC'C
Cuts at: 2903 2903
Size: 5815
ApaLI G'TGCA_C
Cuts at: 3872 5028 3872
Size: 1156 4659
ApoI r'AATT_y
Cuts at: 1493 3124 3350 3502 5611 1493
Size: 1631 226 152 2109 1697
Fragments arranged by size:
2109 1697 1631 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4369 160
Size: 872 3337 1606
Fragments arranged by size:
3337 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5815
AvaI C'yCGr_G
Cuts at: 1870 1943 3140 1870
Size: 73 1197 4545
Fragments arranged by size:
4545 1197 73
BamHI G'GATC_C
Cuts at: 3145 3512 3145
Size: 367 5448
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3136 4591 618
Size: 325 217 627 1349 1455 1842
Fragments arranged by size:
1842 1455 1349 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2903 3134 1391
Size: 1512 231 4072
Fragments arranged by size:
4072 1512 231
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4844
BbvCI CC'TCA_GC
Cuts at: 3041 3041
Size: 5815
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4006 4040 1903
Size: 34 2069 34 3678
Fragments arranged by size:
3678 2069 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3707 4917 3707
Size: 1210 4605
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5815
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4426 136
Size: 107 122 71 631 605 2754 1525
Fragments arranged by size:
2754 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2210 5810 2210
Size: 3600 2215
BlpI GC'TnA_GC
Cuts at: 3232 3232
Size: 5815
Bme1580I G_kGCm'C
Cuts at: 1790 2489 2903 3876 5032 1790
Size: 699 414 973 1156 2573
Fragments arranged by size:
2573 1156 973 699 414
BmgBI CAC'GTC
Cuts at: 1318 2415 2482 1318
Size: 1097 67 4651
Fragments arranged by size:
4651 1097 67
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3546 4504 442
Size: 416 53 83 399 24 2129 958 1753
Fragments arranged by size:
2129 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2738 1056
Size: 1682 4133
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2724 4475 1239
Size: 1485 1751 2579
Fragments arranged by size:
2579 1751 1485
Bpu10I CC'TnA_GC
Cuts at: 3041 3041
Size: 5815
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2597 3940 4805 5103 2597
Size: 1343 865 298 3309
Fragments arranged by size:
3309 1343 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4478 882
Size: 617 2979 2219
Fragments arranged by size:
2979 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3606 493
Size: 711 2402 2702
Fragments arranged by size:
2702 2402 711
BsaBI GATnn'nnATC
Cuts at: 5809 5809
Size: 5815
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3622 4004 4639 275
Size: 53 83 186 936 2089 382 635 1451
Fragments arranged by size:
2089 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4248 4646 4920 5067 5478 4248
Size: 398 274 147 411 4585
Fragments arranged by size:
4585 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2091 2121 1178
Size: 30 165 30 688 30 4872
Fragments arranged by size:
4872 688 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5815
BseYI C'CCAG_C
Cuts at: 2773 5018 5514 2773
Size: 2245 496 3074
Fragments arranged by size:
3074 2245 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5242
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2638 3265 4026 5054 1540
Size: 275 186 637 627 761 1028 2301
Fragments arranged by size:
2301 1028 761 637 627 275 186
BsiHKAI G_wGCw'C
Cuts at: 2167 3876 3961 5032 2167
Size: 1709 85 1071 2950
Fragments arranged by size:
2950 1709 1071 85
BsmI GAATG_Cn'
Cuts at: 3326 3419 3326
Size: 93 5722
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2167 2489 2903 3134 3876 3961 5032
Size: 399 377 322 414 231 742 85 1071
Cuts at: 5032 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 414 399 377 322 231
85
BspEI T'CCGG_A
Cuts at: 5478 5478
Size: 5815
BspHI T'CATG_A
Cuts at: 3704 5434 3704
Size: 1730 4085
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3170 844
Size: 2326 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2535 3702 1394
Size: 429 712 1167 3507
Fragments arranged by size:
3507 1167 712 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4310 4484 65
Size: 1183 3062 174 1396
Fragments arranged by size:
3062 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4459 4646 1700
Size: 110 2649 187 2869
Fragments arranged by size:
2869 2649 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5815
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5815
BssSI C'ACGA_G
Cuts at: 3875 4887 3875
Size: 1012 4803
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5815
BstBI TT'CG_AA
Cuts at: 4619 4619
Size: 5815
BstEII G'GTnAC_C
Cuts at: 1184 3087 1184
Size: 1903 3912
BstXI CCAn_nnnn'nTGG
Cuts at: 2773 2773
Size: 5815
BstYI r'GATC_y
Cuts at: 1972 2185 2210 3145 3512 3897 3914 5366 5452
Size: 213 25 935 367 385 17 1452 86
Cuts at: 5452 5464 5810 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 935 385 367 346 213 86
25 17 12
Bsu36I CC'TnA_GG
Cuts at: 2557 2557
Size: 5815
BtgI C'CryG_G
Cuts at: 513 1065 2004 2457 513
Size: 552 939 453 3871
Fragments arranged by size:
3871 939 552 453
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5076
Fragments arranged by size:
5076 683 56
BtsI GCAGTG_nn'
Cuts at: 2325 3331 4125 4145 2325
Size: 1006 794 20 3995
Fragments arranged by size:
3995 1006 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5815
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2584 2619 2751 2786 529
Size: 35 2020 35 132 35 3558
Fragments arranged by size:
3558 2020 132 35 35 35
DraI TTT'AAA
Cuts at: 3119 3119
Size: 5815
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4822 809
Size: 507 3506 1802
Fragments arranged by size:
3506 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2454 2713 3262
Size: 54 1425 50 275 18 624 259 549
Cuts at: 3262 4151 5780 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 624 549 275 259 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3262 1537
Size: 275 1450 4090
Fragments arranged by size:
4090 1450 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2345 3745 1353
Size: 992 1400 3423
Fragments arranged by size:
3423 1400 992
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4402 4776 4922 387
Size: 1064 138 2813 374 146 1280
Fragments arranged by size:
2813 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2524 2724 3878 4475 5262 5530 1239
Size: 393 892 200 1154 597 787 268 1524
Fragments arranged by size:
1524 1154 892 787 597 393 268 200
EcoO109I rG'GnC_Cy
Cuts at: 2753 2899 2900 2942 2753
Size: 146 1 42 5626
Fragments arranged by size:
5626 146 42 1
EcoRI G'AATT_C
Cuts at: 3124 3502 3124
Size: 378 5437
EcoRV GAT'ATC
Cuts at: 2203 2203
Size: 5815
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3117 3117
Size: 5815
FspI TGC'GCA
Cuts at: 4321 4321
Size: 5815
HaeII r_GCGC'y
Cuts at: 1678 3049 4962 1678
Size: 1371 1913 2531
Fragments arranged by size:
2531 1913 1371
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4456 4488 4530 4562 1373
Size: 32 135 32 2884 32 42 32 2626
Fragments arranged by size:
2884 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3159 3405 669
Size: 988 281 1221 246 3079
Fragments arranged by size:
3079 1221 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5815
HpaI GTT'AAC
Cuts at: 3405 3405
Size: 5815
KpnI G_GTAC'C
Cuts at: 3140 3140
Size: 5815
MfeI C'AATT_G
Cuts at: 2728 3414 2728
Size: 686 5129
MluI A'CGCG_T
Cuts at: 5691 5691
Size: 5815
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2163 2551 4929 5113 1305
Size: 858 388 2378 184 2007
Fragments arranged by size:
2378 2007 858 388 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2456 2715 10
Size: 54 1425 343 624 259 3110
Fragments arranged by size:
3110 1425 624 343 259 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3773 4132 4291 518
Size: 648 2607 359 159 2042
Fragments arranged by size:
2607 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2027 2723 2972 3231 3908 5056 5301 1606
Size: 421 696 249 259 677 1148 245 2120
Fragments arranged by size:
2120 1148 696 677 421 259 249 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2457 513
Size: 552 939 453 3871
Fragments arranged by size:
3871 939 552 453
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5815
NheI G'CTAG_C
Cuts at: 1052 2734 1052
Size: 1682 4133
NotI GC'GGCC_GC
Cuts at: 3262 3262
Size: 5815
NspI r_CATG'y
Cuts at: 1132 1229 3173 4718 1132
Size: 97 1944 1545 2229
Fragments arranged by size:
2229 1944 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5815
PciI A'CATG_T
Cuts at: 4714 4714
Size: 5815
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5815
PfoI T'CCnGG_A
Cuts at: 2701 2701
Size: 5815
PmeI GTTT'AAAC
Cuts at: 3119 3119
Size: 5815
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3884 3916 5397 5429 3884
Size: 32 1481 32 4270
Fragments arranged by size:
4270 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2753 2753
Size: 5815
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5815
PsiI TTA'TAA
Cuts at: 3385 3385
Size: 5815
PspOMI G'GGCC_C
Cuts at: 2899 2899
Size: 5815
PstI C_TGCA'G
Cuts at: 830 3077 3167 830
Size: 2247 90 3478
Fragments arranged by size:
3478 2247 90
PvuI CG_AT'CG
Cuts at: 2001 2638 2001
Size: 637 5178
PvuII CAG'CTG
Cuts at: 2027 2723 2972 2027
Size: 696 249 4870
Fragments arranged by size:
4870 696 249
SalI G'TCGA_C
Cuts at: 1936 3157 1936
Size: 1221 4594
SanDI GG'GwC_CC
Cuts at: 2753 2753
Size: 5815
SapI GCTCTTCn'nnn_
Cuts at: 2345 2345
Size: 5815
SbfI CC_TGCA'GG
Cuts at: 3167 3167
Size: 5815
ScaI AGT'ACT
Cuts at: 4063 5775 4063
Size: 1712 4103
SexAI A'CCwGG_T
Cuts at: 2907 2907
Size: 5815
SfcI C'TryA_G
Cuts at: 826 1045 2014 2946 3073 3163 4298 4979 5170
Size: 219 969 932 127 90 1135 681 191
Cuts at: 5170 826
Size: 1471
Fragments arranged by size:
1471 1135 969 932 681 219 191 127
90
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5815
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5815
SmaI CCC'GGG
Cuts at: 1872 3142 1872
Size: 1270 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2612 3919 4820 5082 820
Size: 197 926 669 1307 901 262 1553
Fragments arranged by size:
1553 1307 926 901 669 262 197
SnaBI TAC'GTA
Cuts at: 493 3606 493
Size: 3113 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5815
SphI G_CATG'C
Cuts at: 1132 3173 1132
Size: 2041 3774
SspI AAT'ATT
Cuts at: 5 52 3739 5
Size: 47 3687 2081
Fragments arranged by size:
3687 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5815
StyI C'CwwG_G
Cuts at: 513 1065 2004 2082 2457 3092 3254 513
Size: 552 939 78 375 635 162 3074
Fragments arranged by size:
3074 939 635 552 375 162 78
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2779 3842 3859 4012 4197 4642 325
Size: 1255 1199 1063 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1199 1063 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4061 5773 96
Size: 274 80 33 51 1443 2084 1712 138
Fragments arranged by size:
2084 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2091 2123 5775 5807 2091
Size: 32 3652 32 2099
Fragments arranged by size:
3652 2099 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2446 1316
Size: 1130 4685
XbaI T'CTAG_A
Cuts at: 3151 3151
Size: 5815
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5815
XmaI C'CCGG_G
Cuts at: 1870 3140 1870
Size: 1270 4545
XmnI GAAnn'nnTTC
Cuts at: 3944 3944
Size: 5815
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3623 276
Size: 53 83 186 936 2089 2468
Fragments arranged by size:
2468 2089 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI
SanDI SapI SbfI ScaI SexAI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PmlI PspXI PsrI RsrII SacI SacII SfiI SfoI
SrfI SwaI XcmI