Restriction map for
pFN21AB4698
(Circular) (Six-base) MAPSORT of: pFN21AB4698.seq Check: 62 from: 1 to: 7354
pFN21AB4698 7354 bp
With 182 enzymes: SgfI *
September 11, 2009 17:03 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5164 278
Size: 53 83 186 936 3628 2468
Fragments arranged by size:
3628 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2664 2888 4697 1937
Size: 727 224 1809 4594
Fragments arranged by size:
4594 1809 727 224
Acc65I G'GTAC_C
Cuts at: 2700 4675 2700
Size: 1975 5379
AclI AA'CG_TT
Cuts at: 1563 4440 5481 5854 1563
Size: 2877 1041 373 3063
Fragments arranged by size:
3063 2877 1041 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2949 5417 6801 7069 1632
Size: 1317 2468 1384 268 1917
Fragments arranged by size:
2468 1917 1384 1317 268
AfeI AGC'GCT
Cuts at: 3326 3326
Size: 7354
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7157
AflIII A'CryG_T
Cuts at: 1203 2689 6253 7230 1203
Size: 1486 3564 977 1327
Fragments arranged by size:
3564 1486 1327 977
AgeI A'CCGG_T
Cuts at: 6185 6185
Size: 7354
AhdI GACnn_n'nnGTC
Cuts at: 2287 6083 2287
Size: 3796 3558
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7354
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2547 1184
Size: 1363 5991
AlwNI CAG_nnn'CTG
Cuts at: 5097 6669 5097
Size: 1572 5782
ApaI G_GGCC'C
Cuts at: 3046 3153 3046
Size: 107 7247
ApaLI G'TGCA_C
Cuts at: 5411 6567 5411
Size: 1156 6198
ApoI r'AATT_y
Cuts at: 1493 2819 3441 3776 4588 4597 4663 4889 5041
Size: 1326 622 335 812 9 66 226 152
Cuts at: 5041 7150 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 1326 812 622 335 226 152
66 9
AseI AT'TA_AT
Cuts at: 160 1032 5908 160
Size: 872 4876 1606
Fragments arranged by size:
4876 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7354
AvaI C'yCGr_G
Cuts at: 1870 1943 3560 4679 1870
Size: 73 1617 1119 4545
Fragments arranged by size:
4545 1617 1119 73
AvrII C'CTAG_G
Cuts at: 2105 2105
Size: 7354
BamHI G'GATC_C
Cuts at: 4283 4684 5051 4283
Size: 401 367 6586
Fragments arranged by size:
6586 401 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2111 2700 3812 4675 6130
Size: 325 217 627 324 589 1112 863 1455
Cuts at: 6130 618
Size: 1842
Fragments arranged by size:
1842 1455 1112 863 627 589 325 324
217
BanII G_rGCy'C
Cuts at: 1391 2410 2869 3046 3138 3153 3210 4104 4673
Size: 1019 459 177 92 15 57 894 569
Cuts at: 4673 1391
Size: 4072
Fragments arranged by size:
4072 1019 894 569 459 177 92 57
15
BbeI G_GCGC'C
Cuts at: 2115 2115
Size: 7354
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 6383
BbvCI CC'TCA_GC
Cuts at: 3975 3975
Size: 7354
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 5545 5579 1903
Size: 34 50 34 3524 34 3678
Fragments arranged by size:
3678 3524 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5246 6456 5246
Size: 1210 6144
BclI T'GATC_A
Cuts at: 1548 3576 3897 1548
Size: 2028 321 5005
Fragments arranged by size:
5005 2028 321
BfrBI ATG'CAT
Cuts at: 2020 2020
Size: 7354
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5965 136
Size: 107 122 71 631 605 4293 1525
Fragments arranged by size:
4293 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2596 3639 4049 7349 2596
Size: 1043 410 3300 2601
Fragments arranged by size:
3300 2601 1043 410
BlpI GC'TnA_GC
Cuts at: 4771 4771
Size: 7354
Bme1580I G_kGCm'C
Cuts at: 1790 3015 3046 3153 4426 5415 6571 1790
Size: 1225 31 107 1273 989 1156 2573
Fragments arranged by size:
2573 1273 1225 1156 989 107 31
BmgBI CAC'GTC
Cuts at: 1318 2329 1318
Size: 1011 6343
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2236 4295 5085
Size: 416 53 83 399 24 819 2059 790
Cuts at: 5085 6043 442
Size: 958 1753
Fragments arranged by size:
2059 1753 958 819 790 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7354
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2621 2883 3190 4467 6014 1239
Size: 1382 262 307 1277 1547 2579
Fragments arranged by size:
2579 1547 1382 1277 307 262
Bpu10I CC'TnA_GC
Cuts at: 2414 3975 2414
Size: 1561 5793
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3257 5479 6344 6642 3257
Size: 2222 865 298 3969
Fragments arranged by size:
3969 2222 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2383 2733 3074 6017 882
Size: 617 884 350 341 2943 2219
Fragments arranged by size:
2943 2219 884 617 350 341
BsaAI yAC'GTr
Cuts at: 493 1204 5145 493
Size: 711 3941 2702
Fragments arranged by size:
3941 2702 711
BsaBI GATnn'nnATC
Cuts at: 3569 3902 7348 3569
Size: 333 3446 3575
Fragments arranged by size:
3575 3446 333
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2112 4338 5161 5543
Size: 53 83 186 936 579 2226 823 382
Cuts at: 5543 6178 275
Size: 635 1451
Fragments arranged by size:
2226 1451 936 823 635 579 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 5787 6185 6459 6606 7017 5787
Size: 398 274 147 411 6124
Fragments arranged by size:
6124 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3453 3483 4010 4040 1178
Size: 30 165 30 2050 30 527 30 4492
Fragments arranged by size:
4492 2050 527 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2268 3743 3898 1186
Size: 1082 1475 155 4642
Fragments arranged by size:
4642 1475 1082 155
BseYI C'CCAG_C
Cuts at: 2345 3004 6557 7053 2345
Size: 659 3553 496 2646
Fragments arranged by size:
3553 2646 659 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6781
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4128 4804 5565 6593 1540
Size: 275 186 2127 676 761 1028 2301
Fragments arranged by size:
2301 2127 1028 761 676 275 186
BsiHKAI G_wGCw'C
Cuts at: 2410 2869 3138 3210 5415 5500 6571 2410
Size: 459 269 72 2205 85 1071 3193
Fragments arranged by size:
3193 2205 1071 459 269 85 72
BsmI GAATG_Cn'
Cuts at: 2986 4561 4865 4958 2986
Size: 1575 304 93 5382
Fragments arranged by size:
5382 1575 304 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2335 2335
Size: 7354
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2410 2869 3015 3046 3138 3153 3210
Size: 399 620 459 146 31 92 15 57
Cuts at: 3210 4104 4426 4673 5415 5500 6571 1391
Size: 894 322 247 742 85 1071 2174
Fragments arranged by size:
2174 1071 894 742 620 459 399 322
247 146 92 85 57 31 15
BspEI T'CCGG_A
Cuts at: 7017 7017
Size: 7354
BspHI T'CATG_A
Cuts at: 3301 3552 3573 5243 6973 3301
Size: 251 21 1670 1730 3682
Fragments arranged by size:
3682 1730 1670 251 21
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4709 844
Size: 3865 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 5241 1394
Size: 429 3418 3507
Fragments arranged by size:
3507 3418 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2712 3325 4491 5849 6023 65
Size: 1183 1464 613 1166 1358 174 1396
Fragments arranged by size:
1464 1396 1358 1183 1166 613 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5998 6185 1700
Size: 110 4188 187 2869
Fragments arranged by size:
4188 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7354
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7354
BssSI C'ACGA_G
Cuts at: 5414 6426 5414
Size: 1012 6342
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2494 4556 794
Size: 1700 2062 3592
Fragments arranged by size:
3592 2062 1700
BstBI TT'CG_AA
Cuts at: 6158 6158
Size: 7354
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7354
BstXI CCAn_nnnn'nTGG
Cuts at: 3268 3268
Size: 7354
BstYI r'GATC_y
Cuts at: 1972 2596 3639 3848 3979 4049 4283 4684 5051
Size: 624 1043 209 131 70 234 401 367
Cuts at: 5051 5436 5453 6905 6991 7003 7349 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1043 624 401 385 367 346
234 209 131 86 70 17 12
BstZ17I GTA'TAC
Cuts at: 2889 2889
Size: 7354
Bsu36I CC'TnA_GG
Cuts at: 2945 2945
Size: 7354
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 6802
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4252 487
Size: 683 56 3026 3589
Fragments arranged by size:
3589 3026 683 56
BtsI GCAGTG_nn'
Cuts at: 2483 3015 4068 4870 5664 5684 2483
Size: 532 1053 802 794 20 4153
Fragments arranged by size:
4153 1053 802 794 532 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7354
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3355 3390 4473 4508 529
Size: 35 2791 35 1083 35 3375
Fragments arranged by size:
3375 2791 1083 35 35 35
DraI TTT'AAA
Cuts at: 4658 4658
Size: 7354
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6361 809
Size: 507 5045 1802
Fragments arranged by size:
5045 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4125 4801 5690
Size: 54 1425 50 275 18 2295 676 889
Cuts at: 5690 7319 8
Size: 1629 43
Fragments arranged by size:
2295 1629 1425 889 676 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4125 4801 1537
Size: 275 2313 676 4090
Fragments arranged by size:
4090 2313 676 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2077 3519 3739 5284 1353
Size: 724 1442 220 1545 3423
Fragments arranged by size:
3423 1545 1442 724 220
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5941 6315 6461 387
Size: 1064 138 4352 374 146 1280
Fragments arranged by size:
4352 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2408 2867 3136 3208 2408
Size: 459 269 72 6554
Fragments arranged by size:
6554 459 269 72
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2621 2883 2949 3190 4467 5417 6014
Size: 393 989 262 66 241 1277 950 597
Cuts at: 6014 6801 7069 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1277 989 950 787 597 393 268
262 241 66
EcoNI CCTnn'n_nnAGG
Cuts at: 3050 3050
Size: 7354
EcoO109I rG'GnC_Cy
Cuts at: 3042 3043 3150 4471 3042
Size: 1 107 1321 5925
Fragments arranged by size:
5925 1321 107 1
EcoRI G'AATT_C
Cuts at: 4588 4663 5041 4588
Size: 75 378 6901
Fragments arranged by size:
6901 378 75
EcoRV GAT'ATC
Cuts at: 3567 3567
Size: 7354
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4233 4233
Size: 7354
FspI TGC'GCA
Cuts at: 5860 5860
Size: 7354
HaeII r_GCGC'y
Cuts at: 1678 2115 3328 6501 1678
Size: 437 1213 3173 2531
Fragments arranged by size:
3173 2531 1213 437
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2273 2305 2382 2414 3943
Size: 32 135 32 701 32 77 32 1529
Cuts at: 3943 3975 4010 4042 5995 6027 6069 6101 1373
Size: 32 35 32 1953 32 42 32 2626
Fragments arranged by size:
2626 1953 1529 701 135 77 42 35
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2838 3346 3393 4698 4944 669
Size: 988 281 900 508 47 1305 246 3079
Fragments arranged by size:
3079 1305 988 900 508 281 246 47
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7354
HpaI GTT'AAC
Cuts at: 4944 4944
Size: 7354
KasI G'GCGC_C
Cuts at: 2111 2111
Size: 7354
KpnI G_GTAC'C
Cuts at: 2704 4679 2704
Size: 1975 5379
MfeI C'AATT_G
Cuts at: 3396 3492 4953 3396
Size: 96 1461 5797
Fragments arranged by size:
5797 1461 96
MluI A'CGCG_T
Cuts at: 7230 7230
Size: 7354
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3439 3850 4158 6468 6652 1305
Size: 2134 411 308 2310 184 2007
Fragments arranged by size:
2310 2134 2007 411 308 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5532
Fragments arranged by size:
5532 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2384 5312 5671 5830 518
Size: 648 1218 2928 359 159 2042
Fragments arranged by size:
2928 2042 1218 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 4770 5447 6595 6840 1606
Size: 3164 677 1148 245 2120
Fragments arranged by size:
3164 2120 1148 677 245
NarI GG'CG_CC
Cuts at: 2112 2112
Size: 7354
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6802
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7354
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7354
NotI GC'GGCC_GC
Cuts at: 4801 4801
Size: 7354
NsiI A_TGCA'T
Cuts at: 2022 2022
Size: 7354
NspI r_CATG'y
Cuts at: 1132 1229 4090 4712 6257 1132
Size: 97 2861 622 1545 2229
Fragments arranged by size:
2861 2229 1545 622 97
PasI CC'CwG_GG
Cuts at: 1348 3015 1348
Size: 1667 5687
PciI A'CATG_T
Cuts at: 6253 6253
Size: 7354
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2360 1108
Size: 1252 6102
PmeI GTTT'AAAC
Cuts at: 4658 4658
Size: 7354
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5423 5455 6936 6968 5423
Size: 32 1481 32 5809
Fragments arranged by size:
5809 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7354
PsiI TTA'TAA
Cuts at: 4611 4924 4611
Size: 313 7041
PspOMI G'GGCC_C
Cuts at: 3042 3149 3042
Size: 107 7247
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2133 2165 2133
Size: 32 7322
PstI C_TGCA'G
Cuts at: 830 3358 4706 830
Size: 2528 1348 3478
Fragments arranged by size:
3478 2528 1348
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7354
SacI G_AGCT'C
Cuts at: 2410 2869 3138 3210 2410
Size: 459 269 72 6554
Fragments arranged by size:
6554 459 269 72
SalI G'TCGA_C
Cuts at: 1936 4696 1936
Size: 2760 4594
SbfI CC_TGCA'GG
Cuts at: 4706 4706
Size: 7354
ScaI AGT'ACT
Cuts at: 5602 7314 5602
Size: 1712 5642
SexAI A'CCwGG_T
Cuts at: 2667 2667
Size: 7354
SfcI C'TryA_G
Cuts at: 826 1045 3354 3942 4702 5837 6518 6709 826
Size: 219 2309 588 760 1135 681 191 1471
Fragments arranged by size:
2309 1471 1135 760 681 588 219 191
SfoI GGC'GCC
Cuts at: 2113 2113
Size: 7354
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7354
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7354
SmaI CCC'GGG
Cuts at: 1872 4681 1872
Size: 2809 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3236 5458 6359 6621 820
Size: 197 926 1293 2222 901 262 1553
Fragments arranged by size:
2222 1553 1293 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5145 493
Size: 4652 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7354
SphI G_CATG'C
Cuts at: 1132 4712 1132
Size: 3580 3774
SspI AAT'ATT
Cuts at: 5 52 4567 5278 5
Size: 47 4515 711 2081
Fragments arranged by size:
4515 2081 711 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7354
StyI C'CwwG_G
Cuts at: 513 1065 2105 3807 4392 4474 4793 513
Size: 552 1040 1702 585 82 319 3074
Fragments arranged by size:
3074 1702 1040 585 552 319 82
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3800 5381 5398 5551 5736 6181 325
Size: 1255 2220 1581 17 153 185 445 1498
Fragments arranged by size:
2220 1581 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3384 4197 5600
Size: 274 80 33 51 1443 1407 813 1403
Cuts at: 5600 7312 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1407 1403 813 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7314 7346 7314
Size: 32 7322
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7354
XbaI T'CTAG_A
Cuts at: 4690 4690
Size: 7354
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3012 3012
Size: 7354
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7354
XmaI C'CCGG_G
Cuts at: 1870 4679 1870
Size: 2809 4545
XmnI GAAnn'nnTTC
Cuts at: 5483 5483
Size: 7354
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5162 276
Size: 53 83 186 936 3628 2468
Fragments arranged by size:
3628 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NarI NcoI
NdeI NheI NotI NsiI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PspOMI PsrI PstI PvuI
SacI SalI SbfI ScaI SexAI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI BaeI BplI BsiWI DraIII FseI
FspAI NaeI NgoMIV NruI PacI PfoI PmlI PpuMI
PspXI PvuII RsrII SacII SanDI SapI SfiI SrfI
SwaI