Restriction map for
pFN21AB4697
(Circular) (Six-base) MAPSORT of: pFN21AB4697.seq Check: 1042 from: 1 to: 5686
pFN21AB4697 5686 bp
With 182 enzymes: SgfI *
October 20, 2009 10:59 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2899 2899
Size: 5686
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3496 278
Size: 53 83 186 936 1960 2468
Fragments arranged by size:
2468 1960 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2478 3029 1937
Size: 541 551 4594
Fragments arranged by size:
4594 551 541
Acc65I G'GTAC_C
Cuts at: 2715 3007 2715
Size: 292 5394
AclI AA'CG_TT
Cuts at: 1563 3813 4186 1563
Size: 2250 373 3063
Fragments arranged by size:
3063 2250 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2813 2957 3749 5133 5401 1632
Size: 1181 144 792 1384 268 1917
Fragments arranged by size:
1917 1384 1181 792 268 144
AflII C'TTAA_G
Cuts at: 820 1017 2669 820
Size: 197 1652 3837
Fragments arranged by size:
3837 1652 197
AflIII A'CryG_T
Cuts at: 1203 2775 4585 5562 1203
Size: 1572 1810 977 1327
Fragments arranged by size:
1810 1572 1327 977
AgeI A'CCGG_T
Cuts at: 4517 4517
Size: 5686
AhdI GACnn_n'nnGTC
Cuts at: 4415 4415
Size: 5686
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5686
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2369 1184
Size: 1185 4501
AlwNI CAG_nnn'CTG
Cuts at: 2035 2935 3429 5001 2035
Size: 900 494 1572 2720
Fragments arranged by size:
2720 1572 900 494
ApaI G_GGCC'C
Cuts at: 2160 2160
Size: 5686
ApaLI G'TGCA_C
Cuts at: 3743 4899 3743
Size: 1156 4530
ApoI r'AATT_y
Cuts at: 1493 2663 2995 3221 3373 5482 1493
Size: 1170 332 226 152 2109 1697
Fragments arranged by size:
2109 1697 1170 332 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4240 160
Size: 872 3208 1606
Fragments arranged by size:
3208 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5686
AvaI C'yCGr_G
Cuts at: 1870 1943 2503 2731 3011 1870
Size: 73 560 228 280 4545
Fragments arranged by size:
4545 560 280 228 73
BamHI G'GATC_C
Cuts at: 3016 3383 3016
Size: 367 5319
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2090 2132 2715 3007 4462
Size: 325 217 627 303 42 583 292 1455
Cuts at: 4462 618
Size: 1842
Fragments arranged by size:
1842 1455 627 583 325 303 292 217
42
BanII G_rGCy'C
Cuts at: 1391 2160 2572 2853 3005 1391
Size: 769 412 281 152 4072
Fragments arranged by size:
4072 769 412 281 152
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2323 2529 2670 928
Size: 971 424 206 141 3944
Fragments arranged by size:
3944 971 424 206 141
BbvCI CC'TCA_GC
Cuts at: 2342 2342
Size: 5686
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2419 2453 3877 3911 1903
Size: 34 482 34 1424 34 3678
Fragments arranged by size:
3678 1424 482 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3578 4788 3578
Size: 1210 4476
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5686
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4297 136
Size: 107 122 71 631 605 2625 1525
Fragments arranged by size:
2625 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5681 5681
Size: 5686
BlpI GC'TnA_GC
Cuts at: 3103 3103
Size: 5686
Bme1580I G_kGCm'C
Cuts at: 1790 2160 2241 3747 4903 1790
Size: 370 81 1506 1156 2573
Fragments arranged by size:
2573 1506 1156 370 81
BmgBI CAC'GTC
Cuts at: 1318 2776 2953 1318
Size: 1458 177 4051
Fragments arranged by size:
4051 1458 177
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3417 4375 442
Size: 416 53 83 399 24 2000 958 1753
Fragments arranged by size:
2000 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5686
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2095 2308 2752 4346 1239
Size: 856 213 444 1594 2579
Fragments arranged by size:
2579 1594 856 444 213
Bpu10I CC'TnA_GC
Cuts at: 2342 2342
Size: 5686
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2728 2840 3811 4676 4974 2728
Size: 112 971 865 298 3440
Fragments arranged by size:
3440 971 865 298 112
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2585 4349 882
Size: 617 1086 1764 2219
Fragments arranged by size:
2219 1764 1086 617
BsaAI yAC'GTr
Cuts at: 493 1204 3477 493
Size: 711 2273 2702
Fragments arranged by size:
2702 2273 711
BsaBI GATnn'nnATC
Cuts at: 5680 5680
Size: 5686
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2601 2675 3493 3875
Size: 53 83 186 936 1068 74 818 382
Cuts at: 3875 4510 275
Size: 635 1451
Fragments arranged by size:
1451 1068 936 818 635 382 186 83
74 53
BsaWI w'CCGG_w
Cuts at: 2247 4119 4517 4791 4938 5349 2247
Size: 1872 398 274 147 411 2584
Fragments arranged by size:
2584 1872 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5461
Fragments arranged by size:
5461 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2294 1186
Size: 1108 4578
BseYI C'CCAG_C
Cuts at: 2362 4889 5385 2362
Size: 2527 496 2663
Fragments arranged by size:
2663 2527 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5113
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2020 2049 3136 3897 4925 1540
Size: 275 186 19 29 1087 761 1028 2301
Fragments arranged by size:
2301 1087 1028 761 275 186 29 19
BsiHKAI G_wGCw'C
Cuts at: 2572 3747 3832 4903 2572
Size: 1175 85 1071 3355
Fragments arranged by size:
3355 1175 1071 85
BsmI GAATG_Cn'
Cuts at: 3197 3290 3197
Size: 93 5593
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2160 2241 2572 2853 3005 3747 3832
Size: 399 370 81 331 281 152 742 85
Cuts at: 3832 4903 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 370 331 281 152
85 81
BspEI T'CCGG_A
Cuts at: 2247 5349 2247
Size: 3102 2584
BspHI T'CATG_A
Cuts at: 3575 5305 3575
Size: 1730 3956
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2899 3041 844
Size: 2055 142 3489
Fragments arranged by size:
3489 2055 142
BsrBI CCG'CTC
Cuts at: 1394 1823 3573 1394
Size: 429 1750 3507
Fragments arranged by size:
3507 1750 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4181 4355 65
Size: 1183 2933 174 1396
Fragments arranged by size:
2933 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2426 2718 4330 4517 1700
Size: 110 616 292 1612 187 2869
Fragments arranged by size:
2869 1612 616 292 187 110
BsrGI T'GTAC_A
Cuts at: 96 2832 96
Size: 2736 2950
BssHII G'CGCG_C
Cuts at: 1925 2115 1925
Size: 190 5496
BssSI C'ACGA_G
Cuts at: 3746 4758 3746
Size: 1012 4674
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2035 794
Size: 1241 4445
BstBI TT'CG_AA
Cuts at: 4490 4490
Size: 5686
BstEII G'GTnAC_C
Cuts at: 1184 2498 2860 1184
Size: 1314 362 4010
Fragments arranged by size:
4010 1314 362
BstXI CCAn_nnnn'nTGG
Cuts at: 2815 2815
Size: 5686
BstYI r'GATC_y
Cuts at: 1972 2226 2391 3016 3383 3768 3785 5237 5323
Size: 254 165 625 367 385 17 1452 86
Cuts at: 5323 5335 5681 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 625 385 367 346 254 165
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2020 2043 2145 2176 2595 2784
Size: 552 939 16 23 102 31 419 189
Cuts at: 2784 2814 513
Size: 30 3385
Fragments arranged by size:
3385 939 552 419 189 102 31 30
23 16
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2026 2065 487
Size: 683 56 800 39 4108
Fragments arranged by size:
4108 800 683 56 39
BtsI GCAGTG_nn'
Cuts at: 2239 3202 3996 4016 2239
Size: 963 794 20 3909
Fragments arranged by size:
3909 963 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5686
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5651
DraI TTT'AAA
Cuts at: 2990 2990
Size: 5686
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4693 809
Size: 507 3377 1802
Fragments arranged by size:
3377 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2046 2076 3133
Size: 54 1425 50 275 18 216 30 1057
Cuts at: 3133 4022 5651 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1057 889 275 216 54 50
43 30 18
EagI C'GGCC_G
Cuts at: 1537 1812 2046 3133 1537
Size: 275 234 1087 4090
Fragments arranged by size:
4090 1087 275 234
EarI CTCTTCn'nnn_
Cuts at: 1353 2441 2689 3616 1353
Size: 1088 248 927 3423
Fragments arranged by size:
3423 1088 927 248
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4273 4647 4793 387
Size: 1064 138 2684 374 146 1280
Fragments arranged by size:
2684 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2570 2570
Size: 5686
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2095 2308 2752 2813 2957 3749 4346
Size: 393 463 213 444 61 144 792 597
Cuts at: 4346 5133 5401 1239
Size: 787 268 1524
Fragments arranged by size:
1524 792 787 597 463 444 393 268
213 144 61
EcoO109I rG'GnC_Cy
Cuts at: 2157 2932 2157
Size: 775 4911
EcoRI G'AATT_C
Cuts at: 2663 2995 3373 2663
Size: 332 378 4976
Fragments arranged by size:
4976 378 332
FspI TGC'GCA
Cuts at: 4192 4192
Size: 5686
HaeII r_GCGC'y
Cuts at: 1678 2731 4833 1678
Size: 1053 2102 2531
Fragments arranged by size:
2531 2102 1053
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4327 4359 4401 4433 1373
Size: 32 135 32 2755 32 42 32 2626
Fragments arranged by size:
2755 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3030 3276 669
Size: 988 281 1092 246 3079
Fragments arranged by size:
3079 1092 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5686
HpaI GTT'AAC
Cuts at: 3276 3276
Size: 5686
KpnI G_GTAC'C
Cuts at: 2719 3011 2719
Size: 292 5394
MfeI C'AATT_G
Cuts at: 3285 3285
Size: 5686
MluI A'CGCG_T
Cuts at: 5562 5562
Size: 5686
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4800 4984 1305
Size: 3495 184 2007
Fragments arranged by size:
3495 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2078 10
Size: 54 1425 343 246 3618
Fragments arranged by size:
3618 1425 343 246 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2813 3644 4003 4162 518
Size: 648 1647 831 359 159 2042
Fragments arranged by size:
2042 1647 831 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2022 2178 3102 3779 4927 5172 1606
Size: 416 156 924 677 1148 245 2120
Fragments arranged by size:
2120 1148 924 677 416 245 156
NaeI GCC'GGC
Cuts at: 2428 2428
Size: 5686
NcoI C'CATG_G
Cuts at: 513 1065 2004 2145 2784 2814 513
Size: 552 939 141 639 30 3385
Fragments arranged by size:
3385 939 639 552 141 30
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5686
NgoMIV G'CCGG_C
Cuts at: 2426 2426
Size: 5686
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5686
NotI GC'GGCC_GC
Cuts at: 3133 3133
Size: 5686
NspI r_CATG'y
Cuts at: 1132 1229 2962 3044 4589 1132
Size: 97 1733 82 1545 2229
Fragments arranged by size:
2229 1733 1545 97 82
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5686
PciI A'CATG_T
Cuts at: 4585 4585
Size: 5686
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5686
PmeI GTTT'AAAC
Cuts at: 2990 2990
Size: 5686
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3755 3787 5268 5300 3755
Size: 32 1481 32 4141
Fragments arranged by size:
4141 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2932 2932
Size: 5686
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5686
PsiI TTA'TAA
Cuts at: 3256 3256
Size: 5686
PspOMI G'GGCC_C
Cuts at: 2156 2156
Size: 5686
PspXI vC'TCGA_Gb
Cuts at: 2731 2731
Size: 5686
PstI C_TGCA'G
Cuts at: 830 2031 2449 2910 3038 830
Size: 1201 418 461 128 3478
Fragments arranged by size:
3478 1201 461 418 128
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5686
SacI G_AGCT'C
Cuts at: 2572 2572
Size: 5686
SacII CC_GC'GG
Cuts at: 2023 2179 2023
Size: 156 5530
SalI G'TCGA_C
Cuts at: 1936 3028 1936
Size: 1092 4594
SbfI CC_TGCA'GG
Cuts at: 2910 3038 2910
Size: 128 5558
ScaI AGT'ACT
Cuts at: 3934 5646 3934
Size: 1712 3974
SfcI C'TryA_G
Cuts at: 826 1045 2027 2395 2445 2710 2878 2906 3034
Size: 219 982 368 50 265 168 28 128
Cuts at: 3034 4169 4850 5041 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1135 982 681 368 265 219 191
168 128 50 28
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5686
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5686
SmaI CCC'GGG
Cuts at: 1872 3013 1872
Size: 1141 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2669 2731 2743 2855 3790 4691
Size: 197 926 726 62 12 112 935 901
Cuts at: 4691 4953 820
Size: 262 1553
Fragments arranged by size:
1553 935 926 901 726 262 197 112
62 12
SnaBI TAC'GTA
Cuts at: 493 3477 493
Size: 2984 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5686
SphI G_CATG'C
Cuts at: 1132 2962 3044 1132
Size: 1830 82 3774
Fragments arranged by size:
3774 1830 82
SspI AAT'ATT
Cuts at: 5 52 3610 5
Size: 47 3558 2081
Fragments arranged by size:
3558 2081 47
StuI AGG'CCT
Cuts at: 1517 2451 2759 1517
Size: 934 308 4444
Fragments arranged by size:
4444 934 308
StyI C'CwwG_G
Cuts at: 513 1065 2004 2145 2784 2814 3125 513
Size: 552 939 141 639 30 311 3074
Fragments arranged by size:
3074 939 639 552 311 141 30
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2204 2354 3713 3730 3883 4068 4513
Size: 1255 624 150 1359 17 153 185 445
Cuts at: 4513 325
Size: 1498
Fragments arranged by size:
1498 1359 1255 624 445 185 153 150
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2106 2832 3932
Size: 274 80 33 51 1443 129 726 1100
Cuts at: 3932 5644 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1100 726 274 138 129 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5646 5678 5646
Size: 32 5654
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5686
XbaI T'CTAG_A
Cuts at: 3022 3022
Size: 5686
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2143 2143
Size: 5686
XhoI C'TCGA_G
Cuts at: 1943 2731 1943
Size: 788 4898
XmaI C'CCGG_G
Cuts at: 1870 3011 1870
Size: 1141 4545
XmnI GAAnn'nnTTC
Cuts at: 3815 3815
Size: 5686
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3494 276
Size: 53 83 186 936 1960 2468
Fragments arranged by size:
2468 1960 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PspOMI PspXI PstI PvuI SacI
SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI
BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI
FseI FspAI KasI NarI NruI NsiI PacI PfoI
PmlI PsrI PvuII RsrII SanDI SapI SexAI SfiI
SfoI SrfI SwaI