Restriction map for
pFN21AB4689
(Circular) (Six-base) MAPSORT of: pFN21AB4689.seq Check: 3079 from: 1 to: 6025
pFN21AB4689 6025 bp
With 182 enzymes: SgfI *
January 16, 2009 15:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3835 278
Size: 53 83 186 936 2299 2468
Fragments arranged by size:
2468 2299 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3368 1937
Size: 1431 4594
Acc65I G'GTAC_C
Cuts at: 3346 3346
Size: 6025
AclI AA'CG_TT
Cuts at: 1563 4152 4525 1563
Size: 2589 373 3063
Fragments arranged by size:
3063 2589 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2135 2327 4088 5472 5740 1632
Size: 503 192 1761 1384 268 1917
Fragments arranged by size:
1917 1761 1384 503 268 192
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5828
AflIII A'CryG_T
Cuts at: 1203 4924 5901 1203
Size: 3721 977 1327
Fragments arranged by size:
3721 1327 977
AgeI A'CCGG_T
Cuts at: 4856 4856
Size: 6025
AhdI GACnn_n'nnGTC
Cuts at: 4754 4754
Size: 6025
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6025
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6025
AlwNI CAG_nnn'CTG
Cuts at: 3768 5340 3768
Size: 1572 4453
ApaLI G'TGCA_C
Cuts at: 2595 4082 5238 2595
Size: 1487 1156 3382
Fragments arranged by size:
3382 1487 1156
ApoI r'AATT_y
Cuts at: 1493 2057 2301 3334 3560 3712 5821 1493
Size: 564 244 1033 226 152 2109 1697
Fragments arranged by size:
2109 1697 1033 564 244 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4579 160
Size: 872 3547 1606
Fragments arranged by size:
3547 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6025
AvaI C'yCGr_G
Cuts at: 1870 1943 2255 3350 1870
Size: 73 312 1095 4545
Fragments arranged by size:
4545 1095 312 73
BamHI G'GATC_C
Cuts at: 3218 3355 3722 3218
Size: 137 367 5521
Fragments arranged by size:
5521 367 137
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3346 4801 618
Size: 325 217 627 1559 1455 1842
Fragments arranged by size:
1842 1559 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2786 2845 2993 3344 1391
Size: 1395 59 148 351 4072
Fragments arranged by size:
4072 1395 351 148 59
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5054
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4216 4250 1903
Size: 34 2279 34 3678
Fragments arranged by size:
3678 2279 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3917 5127 3917
Size: 1210 4815
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6025
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4636 136
Size: 107 122 71 631 605 2964 1525
Fragments arranged by size:
2964 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2108 6020 2108
Size: 3912 2113
BlpI GC'TnA_GC
Cuts at: 3442 3442
Size: 6025
Bme1580I G_kGCm'C
Cuts at: 1790 2599 4086 5242 1790
Size: 809 1487 1156 2573
Fragments arranged by size:
2573 1487 1156 809
BmgBI CAC'GTC
Cuts at: 1318 2594 1318
Size: 1276 4749
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2675 3756 4714
Size: 416 53 83 399 24 1258 1081 958
Cuts at: 4714 442
Size: 1753
Fragments arranged by size:
1753 1258 1081 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6025
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2940 4685 1239
Size: 1701 1745 2579
Fragments arranged by size:
2579 1745 1701
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4150 5015 5313 4150
Size: 865 298 4862
Fragments arranged by size:
4862 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2880 2908 2916 4688 882
Size: 617 1381 28 8 1772 2219
Fragments arranged by size:
2219 1772 1381 617 28 8
BsaAI yAC'GTr
Cuts at: 493 1204 3816 493
Size: 711 2612 2702
Fragments arranged by size:
2702 2612 711
BsaBI GATnn'nnATC
Cuts at: 6019 6019
Size: 6025
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2677 2743 3832 4214
Size: 53 83 186 936 1144 66 1089 382
Cuts at: 4214 4849 275
Size: 635 1451
Fragments arranged by size:
1451 1144 1089 936 635 382 186 83
66 53
BsaWI w'CCGG_w
Cuts at: 3032 4458 4856 5130 5277 5688 3032
Size: 1426 398 274 147 411 3369
Fragments arranged by size:
3369 1426 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5800
Fragments arranged by size:
5800 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2799 1186
Size: 1613 4412
BseYI C'CCAG_C
Cuts at: 2165 5228 5724 2165
Size: 3063 496 2466
Fragments arranged by size:
3063 2466 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5452
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2827 3475 4236 5264 1540
Size: 275 186 826 648 761 1028 2301
Fragments arranged by size:
2301 1028 826 761 648 275 186
BsiHKAI G_wGCw'C
Cuts at: 2599 2845 4086 4171 5242 2599
Size: 246 1241 85 1071 3382
Fragments arranged by size:
3382 1241 1071 246 85
BsmI GAATG_Cn'
Cuts at: 2142 2167 2486 2727 3274 3536 3629 2142
Size: 25 319 241 547 262 93 4538
Fragments arranged by size:
4538 547 319 262 241 93 25
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2599 2786 2845 2993 3344 4086 4171
Size: 399 809 187 59 148 351 742 85
Cuts at: 4171 5242 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 809 742 399 351 187 148
85 59
BspEI T'CCGG_A
Cuts at: 3032 5688 3032
Size: 2656 3369
BspHI T'CATG_A
Cuts at: 3175 3914 5644 3175
Size: 739 1730 3556
Fragments arranged by size:
3556 1730 739
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3380 844
Size: 2536 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3912 1394
Size: 429 2089 3507
Fragments arranged by size:
3507 2089 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4520 4694 65
Size: 1183 3272 174 1396
Fragments arranged by size:
3272 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4669 4856 1700
Size: 110 2859 187 2869
Fragments arranged by size:
2869 2859 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6025
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6025
BssSI C'ACGA_G
Cuts at: 4085 5097 4085
Size: 1012 5013
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6025
BstBI TT'CG_AA
Cuts at: 4829 4829
Size: 6025
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6025
BstXI CCAn_nnnn'nTGG
Cuts at: 3000 3124 3000
Size: 124 5901
BstYI r'GATC_y
Cuts at: 1972 2108 3131 3218 3355 3722 4107 4124 5576
Size: 136 1023 87 137 367 385 17 1452
Cuts at: 5576 5662 5674 6020 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1023 385 367 346 137 136
87 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4534
Fragments arranged by size:
4534 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5286
Fragments arranged by size:
5286 683 56
BtsI GCAGTG_nn'
Cuts at: 2178 2254 3541 4335 4355 2178
Size: 76 1287 794 20 3848
Fragments arranged by size:
3848 1287 794 76 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6025
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5990
DraI TTT'AAA
Cuts at: 2104 3329 2104
Size: 1225 4800
DraIII CAC_nnn'GTG
Cuts at: 3026 3026
Size: 6025
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5032 809
Size: 507 3716 1802
Fragments arranged by size:
3716 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2824 3472 4361
Size: 54 1425 50 275 18 994 648 889
Cuts at: 4361 5990 8
Size: 1629 43
Fragments arranged by size:
1629 1425 994 889 648 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2824 3472 1537
Size: 275 1012 648 4090
Fragments arranged by size:
4090 1012 648 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2082 2325 2698 2758 2862 2902 3955 1353
Size: 729 243 373 60 104 40 1053 3423
Fragments arranged by size:
3423 1053 729 373 243 104 60 40
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4612 4986 5132 387
Size: 1064 138 3023 374 146 1280
Fragments arranged by size:
3023 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2843 2843
Size: 6025
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2135 2327 2940 4088 4685 5472 5740
Size: 393 503 192 613 1148 597 787 268
Cuts at: 5740 1239
Size: 1524
Fragments arranged by size:
1524 1148 787 613 597 503 393 268
192
EcoNI CCTnn'n_nnAGG
Cuts at: 2790 3213 2790
Size: 423 5602
EcoRI G'AATT_C
Cuts at: 3334 3712 3334
Size: 378 5647
FspI TGC'GCA
Cuts at: 4531 4531
Size: 6025
HaeII r_GCGC'y
Cuts at: 1678 5172 1678
Size: 3494 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2555 2587 2773 2805 3177
Size: 32 135 32 983 32 186 32 372
Cuts at: 3177 3209 4666 4698 4740 4772 1373
Size: 32 1457 32 42 32 2626
Fragments arranged by size:
2626 1457 983 372 186 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3369 3615 669
Size: 988 281 1431 246 3079
Fragments arranged by size:
3079 1431 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2468 748
Size: 1720 4305
HpaI GTT'AAC
Cuts at: 3615 3615
Size: 6025
KpnI G_GTAC'C
Cuts at: 3350 3350
Size: 6025
MfeI C'AATT_G
Cuts at: 2292 3624 2292
Size: 1332 4693
MluI A'CGCG_T
Cuts at: 5901 5901
Size: 6025
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5139 5323 1305
Size: 3834 184 2007
Fragments arranged by size:
3834 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4203
Fragments arranged by size:
4203 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2998 3983 4342 4501 518
Size: 648 1832 985 359 159 2042
Fragments arranged by size:
2042 1832 985 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3157 3441 4118 5266 5511 1606
Size: 1551 284 677 1148 245 2120
Fragments arranged by size:
2120 1551 1148 677 284 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4534
Fragments arranged by size:
4534 939 552
NdeI CA'TA_TG
Cuts at: 387 3070 387
Size: 2683 3342
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6025
NotI GC'GGCC_GC
Cuts at: 3472 3472
Size: 6025
NspI r_CATG'y
Cuts at: 1132 1229 3383 4928 1132
Size: 97 2154 1545 2229
Fragments arranged by size:
2229 2154 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6025
PciI A'CATG_T
Cuts at: 4924 4924
Size: 6025
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3026 1108
Size: 1918 4107
PfoI T'CCnGG_A
Cuts at: 2475 2812 2475
Size: 337 5688
PmeI GTTT'AAAC
Cuts at: 3329 3329
Size: 6025
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4094 4126 5607 5639 4094
Size: 32 1481 32 4480
Fragments arranged by size:
4480 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6025
PsiI TTA'TAA
Cuts at: 3595 3595
Size: 6025
PstI C_TGCA'G
Cuts at: 830 2077 3288 3377 830
Size: 1247 1211 89 3478
Fragments arranged by size:
3478 1247 1211 89
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6025
SacI G_AGCT'C
Cuts at: 2845 2845
Size: 6025
SalI G'TCGA_C
Cuts at: 1936 3367 1936
Size: 1431 4594
SbfI CC_TGCA'GG
Cuts at: 3377 3377
Size: 6025
ScaI AGT'ACT
Cuts at: 4273 5985 4273
Size: 1712 4313
SfcI C'TryA_G
Cuts at: 826 1045 2073 3284 3373 4508 5189 5380 826
Size: 219 1028 1211 89 1135 681 191 1471
Fragments arranged by size:
1471 1211 1135 1028 681 219 191 89
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6025
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6025
SmaI CCC'GGG
Cuts at: 1872 3352 1872
Size: 1480 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4129 5030 5292 820
Size: 197 926 2186 901 262 1553
Fragments arranged by size:
2186 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3816 493
Size: 3323 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6025
SphI G_CATG'C
Cuts at: 1132 3383 1132
Size: 2251 3774
SspI AAT'ATT
Cuts at: 5 52 2197 3949 5
Size: 47 2145 1752 2081
Fragments arranged by size:
2145 2081 1752 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6025
StyI C'CwwG_G
Cuts at: 513 1065 2004 2325 3464 513
Size: 552 939 321 1139 3074
Fragments arranged by size:
3074 1139 939 552 321
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4052 4069 4222 4407 4852 325
Size: 1255 2472 17 153 185 445 1498
Fragments arranged by size:
2472 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4271 5983 96
Size: 274 80 33 51 1443 2294 1712 138
Fragments arranged by size:
2294 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2667 2699 5985 6017 2667
Size: 32 3286 32 2675
Fragments arranged by size:
3286 2675 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6025
XbaI T'CTAG_A
Cuts at: 2111 2759 3361 2111
Size: 648 602 4775
Fragments arranged by size:
4775 648 602
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6025
XmaI C'CCGG_G
Cuts at: 1870 3350 1870
Size: 1480 4545
XmnI GAAnn'nnTTC
Cuts at: 4154 4154
Size: 6025
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3833 276
Size: 53 83 186 936 2299 2468
Fragments arranged by size:
2468 2299 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI
SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I
EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI
PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI XcmI