Restriction map for
pFN21AB4680
(Circular) (Six-base) MAPSORT of: pFN21AB4680.seq Check: 4512 from: 1 to: 6271
pFN21AB4680 6271 bp
With 182 enzymes: SgfI *
February 27, 2009 14:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2806 2806
Size: 6271
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4081 278
Size: 53 83 186 936 2545 2468
Fragments arranged by size:
2545 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2026 3614 1937
Size: 89 1588 4594
Fragments arranged by size:
4594 1588 89
Acc65I G'GTAC_C
Cuts at: 3592 3592
Size: 6271
AclI AA'CG_TT
Cuts at: 1563 4398 4771 1563
Size: 2835 373 3063
Fragments arranged by size:
3063 2835 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4334 5718 5986 1632
Size: 2702 1384 268 1917
Fragments arranged by size:
2702 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6074
AflIII A'CryG_T
Cuts at: 1203 2319 2550 5170 6147 1203
Size: 1116 231 2620 977 1327
Fragments arranged by size:
2620 1327 1116 977 231
AgeI A'CCGG_T
Cuts at: 5102 5102
Size: 6271
AhdI GACnn_n'nnGTC
Cuts at: 5000 5000
Size: 6271
AleI CACnn'nnGTG
Cuts at: 1166 2690 1166
Size: 1524 4747
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2828 1184
Size: 1644 4627
AlwNI CAG_nnn'CTG
Cuts at: 2044 2627 2649 3534 4014 5586 2044
Size: 583 22 885 480 1572 2729
Fragments arranged by size:
2729 1572 885 583 480 22
ApaLI G'TGCA_C
Cuts at: 3380 4328 5484 3380
Size: 948 1156 4167
Fragments arranged by size:
4167 1156 948
ApoI r'AATT_y
Cuts at: 1493 2243 3580 3806 3958 6067 1493
Size: 750 1337 226 152 2109 1697
Fragments arranged by size:
2109 1697 1337 750 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4825 160
Size: 872 3793 1606
Fragments arranged by size:
3793 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6271
AvaI C'yCGr_G
Cuts at: 1870 1943 3301 3596 1870
Size: 73 1358 295 4545
Fragments arranged by size:
4545 1358 295 73
BamHI G'GATC_C
Cuts at: 3601 3968 3601
Size: 367 5904
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3592 5047 618
Size: 325 217 627 1805 1455 1842
Fragments arranged by size:
1842 1805 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3098 3590 1391
Size: 1707 492 4072
Fragments arranged by size:
4072 1707 492
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2295 3130 3260 928
Size: 971 396 835 130 3939
Fragments arranged by size:
3939 971 835 396 130
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4462 4496 1903
Size: 34 2525 34 3678
Fragments arranged by size:
3678 2525 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4163 5373 4163
Size: 1210 5061
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6271
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2945 3102 4882
Size: 107 122 71 631 605 1273 157 1780
Cuts at: 4882 136
Size: 1525
Fragments arranged by size:
1780 1525 1273 631 605 157 122 107
71
BglII A'GATC_T
Cuts at: 3108 6266 3108
Size: 3158 3113
BlpI GC'TnA_GC
Cuts at: 3688 3688
Size: 6271
Bme1580I G_kGCm'C
Cuts at: 1790 3122 3234 3384 4332 5488 1790
Size: 1332 112 150 948 1156 2573
Fragments arranged by size:
2573 1332 1156 948 150 112
BmgBI CAC'GTC
Cuts at: 1318 2293 2794 1318
Size: 975 501 4795
Fragments arranged by size:
4795 975 501
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2098 2466 3322
Size: 416 53 83 399 24 681 368 856
Cuts at: 3322 4002 4960 442
Size: 680 958 1753
Fragments arranged by size:
1753 958 856 681 680 416 399 368
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2300 1056
Size: 1244 5027
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2452 2452
Size: 6271
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2249 4931 1239
Size: 1010 2682 2579
Fragments arranged by size:
2682 2579 1010
Bpu10I CC'TnA_GC
Cuts at: 3092 3170 3362 3092
Size: 78 192 6001
Fragments arranged by size:
6001 192 78
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2992 3263 3540 4396 5261 5559 2992
Size: 271 277 856 865 298 3704
Fragments arranged by size:
3704 865 856 298 277 271
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4934 882
Size: 617 3435 2219
Fragments arranged by size:
3435 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3237 4062 493
Size: 711 2033 825 2702
Fragments arranged by size:
2702 2033 825 711
BsaBI GATnn'nnATC
Cuts at: 6265 6265
Size: 6271
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4078 4460 5095 275
Size: 53 83 186 936 2545 382 635 1451
Fragments arranged by size:
2545 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4704 5102 5376 5523 5934 4704
Size: 398 274 147 411 5041
Fragments arranged by size:
5041 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6046
Fragments arranged by size:
6046 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6271
BseYI C'CCAG_C
Cuts at: 2516 3143 5474 5970 2516
Size: 627 2331 496 2817
Fragments arranged by size:
2817 2331 627 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2065 2834 1163
Size: 573 329 769 4600
Fragments arranged by size:
4600 769 573 329
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2538 3721 4482 5510 1540
Size: 275 186 537 1183 761 1028 2301
Fragments arranged by size:
2301 1183 1028 761 537 275 186
BsiHKAI G_wGCw'C
Cuts at: 2912 3176 3242 3384 4332 4417 5488 2912
Size: 264 66 142 948 85 1071 3695
Fragments arranged by size:
3695 1071 948 264 142 85 66
BsmI GAATG_Cn'
Cuts at: 3782 3875 3782
Size: 93 6178
BsmBI CGTCTCn'nnnn_
Cuts at: 3085 3085
Size: 6271
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2912 3098 3122 3176 3234 3242 3384
Size: 399 1122 186 24 54 58 8 142
Cuts at: 3384 3590 4332 4417 5488 1391
Size: 206 742 85 1071 2174
Fragments arranged by size:
2174 1122 1071 742 399 206 186 142
85 58 54 24 8
BspEI T'CCGG_A
Cuts at: 5934 5934
Size: 6271
BspHI T'CATG_A
Cuts at: 4160 5890 4160
Size: 1730 4541
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2806 3414 3626 844
Size: 1962 608 212 3489
Fragments arranged by size:
3489 1962 608 212
BsrBI CCG'CTC
Cuts at: 1394 1823 4158 1394
Size: 429 2335 3507
Fragments arranged by size:
3507 2335 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4766 4940 65
Size: 1183 3518 174 1396
Fragments arranged by size:
3518 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2935 4915 5102 1700
Size: 110 1125 1980 187 2869
Fragments arranged by size:
2869 1980 1125 187 110
BsrGI T'GTAC_A
Cuts at: 96 3270 3447 96
Size: 3174 177 2920
Fragments arranged by size:
3174 2920 177
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6271
BssSI C'ACGA_G
Cuts at: 3287 4331 5343 3287
Size: 1044 1012 4215
Fragments arranged by size:
4215 1044 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3330 3414 794
Size: 2536 84 3651
Fragments arranged by size:
3651 2536 84
BstBI TT'CG_AA
Cuts at: 5075 5075
Size: 6271
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6271
BstYI r'GATC_y
Cuts at: 1972 3108 3162 3601 3968 4353 4370 5822 5908
Size: 1136 54 439 367 385 17 1452 86
Cuts at: 5908 5920 6266 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1136 439 385 367 346 86
54 17 12
Bsu36I CC'TnA_GG
Cuts at: 2954 2954
Size: 6271
BtgI C'CryG_G
Cuts at: 513 1065 2004 2370 3099 3375 513
Size: 552 939 366 729 276 3409
Fragments arranged by size:
3409 939 729 552 366 276
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5532
Fragments arranged by size:
5532 683 56
BtsI GCAGTG_nn'
Cuts at: 3787 4581 4601 3787
Size: 794 20 5457
Fragments arranged by size:
5457 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6271
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6236
DraI TTT'AAA
Cuts at: 3575 3575
Size: 6271
DraIII CAC_nnn'GTG
Cuts at: 3330 3330
Size: 6271
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2732 5278 809
Size: 507 1416 2546 1802
Fragments arranged by size:
2546 1802 1416 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2535 3718 4607
Size: 54 1425 50 275 18 705 1183 889
Cuts at: 4607 6236 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1183 889 705 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2535 3718 1537
Size: 275 723 1183 4090
Fragments arranged by size:
4090 1183 723 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2788 4201 1353
Size: 1435 1413 3423
Fragments arranged by size:
3423 1435 1413
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2613 4858 5232 5378 387
Size: 1064 138 1024 2245 374 146 1280
Fragments arranged by size:
2245 1280 1064 1024 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2249 4334 4931 5718 5986 1239
Size: 393 617 2085 597 787 268 1524
Fragments arranged by size:
2085 1524 787 617 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2692 2813 2957 2692
Size: 121 144 6006
Fragments arranged by size:
6006 144 121
EcoO109I rG'GnC_Cy
Cuts at: 3442 3442
Size: 6271
EcoRI G'AATT_C
Cuts at: 2243 3580 3958 2243
Size: 1337 378 4556
Fragments arranged by size:
4556 1337 378
FspI TGC'GCA
Cuts at: 4777 4777
Size: 6271
HaeII r_GCGC'y
Cuts at: 1678 3286 5418 1678
Size: 1608 2132 2531
Fragments arranged by size:
2531 2132 1608
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2654 2686 4912 4944 4986
Size: 32 135 32 1082 32 2226 32 42
Cuts at: 4986 5018 1373
Size: 32 2626
Fragments arranged by size:
2626 2226 1082 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2713 3226 3615 3861 669
Size: 988 281 775 513 389 246 3079
Fragments arranged by size:
3079 988 775 513 389 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6271
HpaI GTT'AAC
Cuts at: 3861 3861
Size: 6271
KpnI G_GTAC'C
Cuts at: 3596 3596
Size: 6271
MfeI C'AATT_G
Cuts at: 3870 3870
Size: 6271
MluI A'CGCG_T
Cuts at: 6147 6147
Size: 6271
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3108 5385 5569 1305
Size: 1803 2277 184 2007
Fragments arranged by size:
2277 2007 1803 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4449
Fragments arranged by size:
4449 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2322 2555 2690 4229 4588 4747 518
Size: 648 1156 233 135 1539 359 159 2042
Fragments arranged by size:
2042 1539 1156 648 359 233 159 135
MspA1I CmG'CkG
Cuts at: 1606 2173 2303 2627 3101 3687 4364 5512 5757
Size: 567 130 324 474 586 677 1148 245
Cuts at: 5757 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 586 567 474 324 245
130
NaeI GCC'GGC
Cuts at: 2937 2937
Size: 6271
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4780
Fragments arranged by size:
4780 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6271
NgoMIV G'CCGG_C
Cuts at: 2935 2935
Size: 6271
NheI G'CTAG_C
Cuts at: 1052 2296 1052
Size: 1244 5027
NotI GC'GGCC_GC
Cuts at: 3718 3718
Size: 6271
NspI r_CATG'y
Cuts at: 1132 1229 2146 2323 2554 3271 3324 3629 5174
Size: 97 917 177 231 717 53 305 1545
Cuts at: 5174 1132
Size: 2229
Fragments arranged by size:
2229 1545 917 717 305 231 177 97
53
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6271
PciI A'CATG_T
Cuts at: 2319 2550 5170 2319
Size: 231 2620 3420
Fragments arranged by size:
3420 2620 231
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3376 1108
Size: 2268 4003
PmeI GTTT'AAAC
Cuts at: 3575 3575
Size: 6271
PmlI CAC'GTG
Cuts at: 3237 3237
Size: 6271
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2175 2207 3410 3442 4340 4372 5853 5885 2175
Size: 32 1203 32 898 32 1481 32 2561
Fragments arranged by size:
2561 1481 1203 898 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3442 3442
Size: 6271
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6271
PsiI TTA'TAA
Cuts at: 3841 3841
Size: 6271
PstI C_TGCA'G
Cuts at: 830 2654 3162 3623 830
Size: 1824 508 461 3478
Fragments arranged by size:
3478 1824 508 461
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6271
PvuII CAG'CTG
Cuts at: 2173 2303 2627 2173
Size: 130 324 5817
Fragments arranged by size:
5817 324 130
SacII CC_GC'GG
Cuts at: 3102 3102
Size: 6271
SalI G'TCGA_C
Cuts at: 1936 3613 1936
Size: 1677 4594
SbfI CC_TGCA'GG
Cuts at: 3623 3623
Size: 6271
ScaI AGT'ACT
Cuts at: 3350 4519 6231 3350
Size: 1169 1712 3390
Fragments arranged by size:
3390 1712 1169
SexAI A'CCwGG_T
Cuts at: 2808 2808
Size: 6271
SfcI C'TryA_G
Cuts at: 826 1045 2036 2650 3158 3619 4754 5435 5626
Size: 219 991 614 508 461 1135 681 191
Cuts at: 5626 826
Size: 1471
Fragments arranged by size:
1471 1135 991 681 614 508 461 219
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6271
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6271
SmaI CCC'GGG
Cuts at: 1872 3598 1872
Size: 1726 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2971 3278 3555 4375 5276 5538
Size: 197 926 1028 307 277 820 901 262
Cuts at: 5538 820
Size: 1553
Fragments arranged by size:
1553 1028 926 901 820 307 277 262
197
SnaBI TAC'GTA
Cuts at: 493 4062 493
Size: 3569 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6271
SphI G_CATG'C
Cuts at: 1132 3629 1132
Size: 2497 3774
SspI AAT'ATT
Cuts at: 5 52 4195 5
Size: 47 4143 2081
Fragments arranged by size:
4143 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6271
StyI C'CwwG_G
Cuts at: 513 1065 2004 3710 513
Size: 552 939 1706 3074
Fragments arranged by size:
3074 1706 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2956 3341 3484 4298 4315 4468 4653
Size: 1255 1376 385 143 814 17 153 185
Cuts at: 4653 5098 325
Size: 445 1498
Fragments arranged by size:
1498 1376 1255 814 445 385 185 153
143 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2125 3270 3348
Size: 274 80 33 51 1443 148 1145 78
Cuts at: 3348 3447 4517 6229 96
Size: 99 1070 1712 138
Fragments arranged by size:
1712 1443 1145 1070 274 148 138 99
80 78 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6231 6263 6231
Size: 32 6239
Tth111I GACn'n_nGTC
Cuts at: 1316 2330 2423 2966 1316
Size: 1014 93 543 4621
Fragments arranged by size:
4621 1014 543 93
XbaI T'CTAG_A
Cuts at: 3607 3607
Size: 6271
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6271
XmaI C'CCGG_G
Cuts at: 1870 3596 1870
Size: 1726 4545
XmnI GAAnn'nnTTC
Cuts at: 3257 4400 3257
Size: 1143 5128
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4079 276
Size: 53 83 186 936 2545 2468
Fragments arranged by size:
2545 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PmeI
PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII
SacII SalI SbfI ScaI SexAI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BsiWI BstXI BstZ17I EcoICRI EcoRV FalI FseI
FspAI KasI NarI NruI NsiI PacI PfoI PspOMI
PspXI PsrI RsrII SacI SanDI SapI SfiI SfoI
SrfI SwaI XcmI