Restriction map for
pFN21AB4666
(Circular) (Six-base) MAPSORT of: pFN21AB4666.seq Check: 4635 from: 1 to: 4909
pFN21AB4666 4909 bp
With 182 enzymes: SgfI *
January 16, 2009 15:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2719 278
Size: 53 83 186 936 1183 2468
Fragments arranged by size:
2468 1183 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2252 1937
Size: 315 4594
Acc65I G'GTAC_C
Cuts at: 2230 2230
Size: 4909
AclI AA'CG_TT
Cuts at: 1563 3036 3409 1563
Size: 1473 373 3063
Fragments arranged by size:
3063 1473 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2972 4356 4624 1632
Size: 1340 1384 268 1917
Fragments arranged by size:
1917 1384 1340 268
AfeI AGC'GCT
Cuts at: 2039 2039
Size: 4909
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 4712
AflIII A'CryG_T
Cuts at: 1203 3808 4785 1203
Size: 2605 977 1327
Fragments arranged by size:
2605 1327 977
AgeI A'CCGG_T
Cuts at: 3740 3740
Size: 4909
AhdI GACnn_n'nnGTC
Cuts at: 3638 3638
Size: 4909
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 4909
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 4909
AlwNI CAG_nnn'CTG
Cuts at: 2652 4224 2652
Size: 1572 3337
ApaLI G'TGCA_C
Cuts at: 2966 4122 2966
Size: 1156 3753
ApoI r'AATT_y
Cuts at: 1493 2218 2444 2596 4705 1493
Size: 725 226 152 2109 1697
Fragments arranged by size:
2109 1697 725 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3463 160
Size: 872 2431 1606
Fragments arranged by size:
2431 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 4909
AvaI C'yCGr_G
Cuts at: 1870 1943 2234 1870
Size: 73 291 4545
Fragments arranged by size:
4545 291 73
BamHI G'GATC_C
Cuts at: 2239 2606 2239
Size: 367 4542
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2230 3685 618
Size: 325 217 627 443 1455 1842
Fragments arranged by size:
1842 1455 627 443 325 217
BanII G_rGCy'C
Cuts at: 1391 2228 1391
Size: 837 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 3938
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2014 2048 3100 3134 1903
Size: 34 77 34 1052 34 3678
Fragments arranged by size:
3678 1052 77 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2801 4011 2801
Size: 1210 3699
BclI T'GATC_A
Cuts at: 1548 1548
Size: 4909
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3520 136
Size: 107 122 71 631 605 1848 1525
Fragments arranged by size:
1848 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 4904 4904
Size: 4909
BlpI GC'TnA_GC
Cuts at: 2326 2326
Size: 4909
Bme1580I G_kGCm'C
Cuts at: 1790 2970 4126 1790
Size: 1180 1156 2573
Fragments arranged by size:
2573 1180 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 4909
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2640 3598 442
Size: 416 53 83 399 24 1223 958 1753
Fragments arranged by size:
1753 1223 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2022 1056
Size: 966 3943
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3569 1239
Size: 2330 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2039 3034 3899 4197 2039
Size: 995 865 298 2751
Fragments arranged by size:
2751 995 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2092 3572 882
Size: 617 593 1480 2219
Fragments arranged by size:
2219 1480 617 593
BsaAI yAC'GTr
Cuts at: 493 1204 2700 493
Size: 711 1496 2702
Fragments arranged by size:
2702 1496 711
BsaBI GATnn'nnATC
Cuts at: 4903 4903
Size: 4909
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2716 3098 3733 275
Size: 53 83 186 936 1183 382 635 1451
Fragments arranged by size:
1451 1183 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3342 3740 4014 4161 4572 3342
Size: 398 274 147 411 3679
Fragments arranged by size:
3679 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 4684
Fragments arranged by size:
4684 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 4909
BseYI C'CCAG_C
Cuts at: 4112 4608 4112
Size: 496 4413
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4336
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2359 3120 4148 1540
Size: 275 186 358 761 1028 2301
Fragments arranged by size:
2301 1028 761 358 275 186
BsiHKAI G_wGCw'C
Cuts at: 2970 3055 4126 2970
Size: 85 1071 3753
Fragments arranged by size:
3753 1071 85
BsmI GAATG_Cn'
Cuts at: 2134 2420 2513 2134
Size: 286 93 4530
Fragments arranged by size:
4530 286 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2228 2970 3055 4126 1391
Size: 399 438 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 438 399 85
BspEI T'CCGG_A
Cuts at: 4572 4572
Size: 4909
BspHI T'CATG_A
Cuts at: 2798 4528 2798
Size: 1730 3179
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2264 844
Size: 1420 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2796 1394
Size: 429 973 3507
Fragments arranged by size:
3507 973 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3404 3578 65
Size: 1183 2156 174 1396
Fragments arranged by size:
2156 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3553 3740 1700
Size: 110 1743 187 2869
Fragments arranged by size:
2869 1743 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 4909
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 4909
BssSI C'ACGA_G
Cuts at: 2969 3981 2969
Size: 1012 3897
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 4909
BstBI TT'CG_AA
Cuts at: 3713 3713
Size: 4909
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 4909
BstYI r'GATC_y
Cuts at: 1972 2192 2239 2606 2991 3008 4460 4546 4558
Size: 220 47 367 385 17 1452 86 12
Cuts at: 4558 4904 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 385 367 346 220 86 47
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4357
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4170
Fragments arranged by size:
4170 683 56
BtsI GCAGTG_nn'
Cuts at: 2425 3219 3239 2425
Size: 794 20 4095
Fragments arranged by size:
4095 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 4909
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 4874
DraI TTT'AAA
Cuts at: 2213 2213
Size: 4909
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3916 809
Size: 507 2600 1802
Fragments arranged by size:
2600 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2356 3245 4874
Size: 54 1425 50 275 18 526 889 1629
Cuts at: 4874 8
Size: 43
Fragments arranged by size:
1629 1425 889 526 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2356 1537
Size: 275 544 4090
Fragments arranged by size:
4090 544 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2839 1353
Size: 1486 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3496 3870 4016 387
Size: 1064 138 1907 374 146 1280
Fragments arranged by size:
1907 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2972 3569 4356 4624 1239
Size: 393 1340 597 787 268 1524
Fragments arranged by size:
1524 1340 787 597 393 268
EcoRI G'AATT_C
Cuts at: 2218 2596 2218
Size: 378 4531
FspI TGC'GCA
Cuts at: 3415 3415
Size: 4909
HaeII r_GCGC'y
Cuts at: 1678 2031 2041 4056 1678
Size: 353 10 2015 2531
Fragments arranged by size:
2531 2015 353 10
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2177 2209 3550 3582 3624
Size: 32 135 32 605 32 1341 32 42
Cuts at: 3624 3656 1373
Size: 32 2626
Fragments arranged by size:
2626 1341 605 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2253 2499 669
Size: 988 281 315 246 3079
Fragments arranged by size:
3079 988 315 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 4909
HpaI GTT'AAC
Cuts at: 2499 2499
Size: 4909
KpnI G_GTAC'C
Cuts at: 2234 2234
Size: 4909
MfeI C'AATT_G
Cuts at: 2508 2508
Size: 4909
MluI A'CGCG_T
Cuts at: 4785 4785
Size: 4909
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2040 4023 4207 1305
Size: 735 1983 184 2007
Fragments arranged by size:
2007 1983 735 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3087
Fragments arranged by size:
3087 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2867 3226 3385 518
Size: 648 1701 359 159 2042
Fragments arranged by size:
2042 1701 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2099 2165 2325 3002 4150 4395 1606
Size: 493 66 160 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 493 245 160 66
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4357
NdeI CA'TA_TG
Cuts at: 387 387
Size: 4909
NheI G'CTAG_C
Cuts at: 1052 2018 1052
Size: 966 3943
NotI GC'GGCC_GC
Cuts at: 2356 2356
Size: 4909
NspI r_CATG'y
Cuts at: 1132 1229 2267 3812 1132
Size: 97 1038 1545 2229
Fragments arranged by size:
2229 1545 1038 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 4909
PciI A'CATG_T
Cuts at: 3808 3808
Size: 4909
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 4909
PfoI T'CCnGG_A
Cuts at: 2181 2181
Size: 4909
PmeI GTTT'AAAC
Cuts at: 2213 2213
Size: 4909
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2978 3010 4491 4523 2978
Size: 32 1481 32 3364
Fragments arranged by size:
3364 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 4909
PsiI TTA'TAA
Cuts at: 2479 2479
Size: 4909
PstI C_TGCA'G
Cuts at: 830 2037 2104 2261 830
Size: 1207 67 157 3478
Fragments arranged by size:
3478 1207 157 67
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 4909
PvuII CAG'CTG
Cuts at: 2099 2165 2099
Size: 66 4843
SalI G'TCGA_C
Cuts at: 1936 2251 1936
Size: 315 4594
SbfI CC_TGCA'GG
Cuts at: 2261 2261
Size: 4909
ScaI AGT'ACT
Cuts at: 2117 3157 4869 2117
Size: 1040 1712 2157
Fragments arranged by size:
2157 1712 1040
SfcI C'TryA_G
Cuts at: 826 1045 2033 2100 2257 3392 4073 4264 826
Size: 219 988 67 157 1135 681 191 1471
Fragments arranged by size:
1471 1135 988 681 219 191 157 67
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 4909
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 4909
SmaI CCC'GGG
Cuts at: 1872 2236 1872
Size: 364 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2054 3013 3914 4176 820
Size: 197 926 111 959 901 262 1553
Fragments arranged by size:
1553 959 926 901 262 197 111
SnaBI TAC'GTA
Cuts at: 493 2700 493
Size: 2207 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 4909
SphI G_CATG'C
Cuts at: 1132 2267 1132
Size: 1135 3774
SspI AAT'ATT
Cuts at: 5 52 2833 5
Size: 47 2781 2081
Fragments arranged by size:
2781 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 4909
StyI C'CwwG_G
Cuts at: 513 1065 2348 513
Size: 552 1283 3074
Fragments arranged by size:
3074 1283 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2144 2936 2953 3106 3291 3736 325
Size: 1255 564 792 17 153 185 445 1498
Fragments arranged by size:
1498 1255 792 564 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2115 3155 4867
Size: 274 80 33 51 1443 138 1040 1712
Cuts at: 4867 96
Size: 138
Fragments arranged by size:
1712 1443 1040 274 138 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4869 4901 4869
Size: 32 4877
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 4909
XbaI T'CTAG_A
Cuts at: 2245 2245
Size: 4909
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 4909
XmaI C'CCGG_G
Cuts at: 1870 2234 1870
Size: 364 4545
XmnI GAAnn'nnTTC
Cuts at: 3038 3038
Size: 4909
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2717 276
Size: 53 83 186 936 1183 2468
Fragments arranged by size:
2468 1183 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI
PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI XcmI