Restriction map for
pFN21AB4662
(Circular) (Six-base) MAPSORT of: pFN21AB4662.seq Check: 4910 from: 1 to: 5887
pFN21AB4662 5887 bp
With 182 enzymes: SgfI *
October 20, 2009 10:34 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3697 278
Size: 53 83 186 936 2161 2468
Fragments arranged by size:
2468 2161 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2475 2924 3230 1937
Size: 538 449 306 4594
Fragments arranged by size:
4594 538 449 306
Acc65I G'GTAC_C
Cuts at: 3208 3208
Size: 5887
AclI AA'CG_TT
Cuts at: 1563 4014 4387 1563
Size: 2451 373 3063
Fragments arranged by size:
3063 2451 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3950 5334 5602 1632
Size: 2318 1384 268 1917
Fragments arranged by size:
2318 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5690
AflIII A'CryG_T
Cuts at: 1203 4786 5763 1203
Size: 3583 977 1327
Fragments arranged by size:
3583 1327 977
AgeI A'CCGG_T
Cuts at: 4718 4718
Size: 5887
AhdI GACnn_n'nnGTC
Cuts at: 4616 4616
Size: 5887
AleI CACnn'nnGTG
Cuts at: 1166 2141 1166
Size: 975 4912
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5887
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2082 2114 2082
Size: 32 5855
AlwNI CAG_nnn'CTG
Cuts at: 2701 3630 5202 2701
Size: 929 1572 3386
Fragments arranged by size:
3386 1572 929
ApaLI G'TGCA_C
Cuts at: 3944 5100 3944
Size: 1156 4731
ApoI r'AATT_y
Cuts at: 1493 2891 3020 3196 3422 3574 5683 1493
Size: 1398 129 176 226 152 2109 1697
Fragments arranged by size:
2109 1697 1398 226 176 152 129
AseI AT'TA_AT
Cuts at: 160 1032 4441 160
Size: 872 3409 1606
Fragments arranged by size:
3409 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5887
AvaI C'yCGr_G
Cuts at: 1870 1943 3212 1870
Size: 73 1269 4545
Fragments arranged by size:
4545 1269 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3119 3152 3119
Size: 33 5854
BamHI G'GATC_C
Cuts at: 3217 3584 3217
Size: 367 5520
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2045 3208 4663 618
Size: 325 217 627 258 1163 1455 1842
Fragments arranged by size:
1842 1455 1163 627 325 258 217
BanII G_rGCy'C
Cuts at: 1391 3206 1391
Size: 1815 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2122 3045 928
Size: 971 223 923 3770
Fragments arranged by size:
3770 971 923 223
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4078 4112 1903
Size: 34 2141 34 3678
Fragments arranged by size:
3678 2141 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2600 2943 3779 4989 2600
Size: 343 836 1210 3498
Fragments arranged by size:
3498 1210 836 343
BclI T'GATC_A
Cuts at: 1548 2814 1548
Size: 1266 4621
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2426 4498 136
Size: 107 122 71 631 605 754 2072 1525
Fragments arranged by size:
2072 1525 754 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2885 5882 2885
Size: 2997 2890
BlpI GC'TnA_GC
Cuts at: 3304 3304
Size: 5887
Bme1580I G_kGCm'C
Cuts at: 1790 3948 5104 1790
Size: 2158 1156 2573
Fragments arranged by size:
2573 2158 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5887
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2501 3618 4576
Size: 416 53 83 399 24 1084 1117 958
Cuts at: 4576 442
Size: 1753
Fragments arranged by size:
1753 1117 1084 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5887
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2502 2937 4547 1239
Size: 1263 435 1610 2579
Fragments arranged by size:
2579 1610 1263 435
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2433 4012 4877 5175 2433
Size: 1579 865 298 3145
Fragments arranged by size:
3145 1579 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4550 882
Size: 617 3051 2219
Fragments arranged by size:
3051 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2671 3678 493
Size: 711 1467 1007 2702
Fragments arranged by size:
2702 1467 1007 711
BsaBI GATnn'nnATC
Cuts at: 5881 5881
Size: 5887
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3694 4076 4711 275
Size: 53 83 186 936 2161 382 635 1451
Fragments arranged by size:
2161 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2405 4320 4718 4992 5139 5550 2405
Size: 1915 398 274 147 411 2742
Fragments arranged by size:
2742 1915 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2323 2353 2475 2505 2820
Size: 30 165 30 920 30 122 30 315
Cuts at: 2820 2850 1178
Size: 30 4215
Fragments arranged by size:
4215 920 315 165 122 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5887
BseYI C'CCAG_C
Cuts at: 2101 2558 5090 5586 2101
Size: 457 2532 496 2402
Fragments arranged by size:
2532 2402 496 457
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3099 1163
Size: 573 1363 3951
Fragments arranged by size:
3951 1363 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3337 4098 5126 1540
Size: 275 186 1336 761 1028 2301
Fragments arranged by size:
2301 1336 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3948 4033 5104 3948
Size: 85 1071 4731
Fragments arranged by size:
4731 1071 85
BsmI GAATG_Cn'
Cuts at: 3398 3491 3398
Size: 93 5794
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3206 3948 4033 5104 1391
Size: 399 1416 742 85 1071 2174
Fragments arranged by size:
2174 1416 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5550 5550
Size: 5887
BspHI T'CATG_A
Cuts at: 3096 3776 5506 3096
Size: 680 1730 3477
Fragments arranged by size:
3477 1730 680
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3242 844
Size: 2398 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3774 1394
Size: 429 1951 3507
Fragments arranged by size:
3507 1951 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4382 4556 65
Size: 1183 3134 174 1396
Fragments arranged by size:
3134 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4531 4718 1700
Size: 110 2721 187 2869
Fragments arranged by size:
2869 2721 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5887
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5887
BssSI C'ACGA_G
Cuts at: 3947 4959 3947
Size: 1012 4875
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5887
BstBI TT'CG_AA
Cuts at: 4691 4691
Size: 5887
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5887
BstXI CCAn_nnnn'nTGG
Cuts at: 2143 2548 2143
Size: 405 5482
BstYI r'GATC_y
Cuts at: 1972 2164 2885 3027 3217 3584 3969 3986 5438
Size: 192 721 142 190 367 385 17 1452
Cuts at: 5438 5524 5536 5882 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 721 385 367 346 192 190
142 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4396
Fragments arranged by size:
4396 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5148
Fragments arranged by size:
5148 683 56
BtsI GCAGTG_nn'
Cuts at: 3403 4197 4217 3403
Size: 794 20 5073
Fragments arranged by size:
5073 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5887
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5852
DraI TTT'AAA
Cuts at: 3191 3191
Size: 5887
DraIII CAC_nnn'GTG
Cuts at: 2908 2908
Size: 5887
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4894 809
Size: 507 3578 1802
Fragments arranged by size:
3578 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3334 4223 5852
Size: 54 1425 50 275 18 1504 889 1629
Cuts at: 5852 8
Size: 43
Fragments arranged by size:
1629 1504 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3334 1537
Size: 275 1522 4090
Fragments arranged by size:
4090 1522 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3817 1353
Size: 2464 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4474 4848 4994 387
Size: 1064 138 2885 374 146 1280
Fragments arranged by size:
2885 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2502 2937 3950 4547 5334 5602 1239
Size: 393 870 435 1013 597 787 268 1524
Fragments arranged by size:
1524 1013 870 787 597 435 393 268
EcoRI G'AATT_C
Cuts at: 2891 3196 3574 2891
Size: 305 378 5204
Fragments arranged by size:
5204 378 305
EcoRV GAT'ATC
Cuts at: 2579 3094 2579
Size: 515 5372
FspI TGC'GCA
Cuts at: 4393 4393
Size: 5887
HaeII r_GCGC'y
Cuts at: 1678 5034 1678
Size: 3356 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2109 2141 2568 2600 2742
Size: 32 135 32 537 32 427 32 142
Cuts at: 2742 2774 2870 2902 4528 4560 4602 4634 1373
Size: 32 96 32 1626 32 42 32 2626
Fragments arranged by size:
2626 1626 537 427 142 135 96 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2215 3231 3477 669
Size: 988 281 277 1016 246 3079
Fragments arranged by size:
3079 1016 988 281 277 246
HindIII A'AGCT_T
Cuts at: 748 2793 748
Size: 2045 3842
HpaI GTT'AAC
Cuts at: 2215 3477 2215
Size: 1262 4625
KpnI G_GTAC'C
Cuts at: 3212 3212
Size: 5887
MfeI C'AATT_G
Cuts at: 3486 3486
Size: 5887
MluI A'CGCG_T
Cuts at: 5763 5763
Size: 5887
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2092 2934 5001 5185 1305
Size: 787 842 2067 184 2007
Fragments arranged by size:
2067 2007 842 787 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4065
Fragments arranged by size:
4065 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2141 3845 4204 4363 518
Size: 648 975 1704 359 159 2042
Fragments arranged by size:
2042 1704 975 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2870 3303 3980 5128 5373 1606
Size: 1264 433 677 1148 245 2120
Fragments arranged by size:
2120 1264 1148 677 433 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4396
Fragments arranged by size:
4396 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5887
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5887
NotI GC'GGCC_GC
Cuts at: 3334 3334
Size: 5887
NspI r_CATG'y
Cuts at: 1132 1229 3245 4790 1132
Size: 97 2016 1545 2229
Fragments arranged by size:
2229 2016 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5887
PciI A'CATG_T
Cuts at: 4786 4786
Size: 5887
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5887
PmeI GTTT'AAAC
Cuts at: 3191 3191
Size: 5887
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3956 3988 5469 5501 3956
Size: 32 1481 32 4342
Fragments arranged by size:
4342 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5887
PsiI TTA'TAA
Cuts at: 3457 3457
Size: 5887
PstI C_TGCA'G
Cuts at: 830 3239 830
Size: 2409 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5887
PvuII CAG'CTG
Cuts at: 2870 2870
Size: 5887
SalI G'TCGA_C
Cuts at: 1936 3229 1936
Size: 1293 4594
SbfI CC_TGCA'GG
Cuts at: 3239 3239
Size: 5887
ScaI AGT'ACT
Cuts at: 4135 5847 4135
Size: 1712 4175
SfcI C'TryA_G
Cuts at: 826 1045 3235 4370 5051 5242 826
Size: 219 2190 1135 681 191 1471
Fragments arranged by size:
2190 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5887
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5887
SmaI CCC'GGG
Cuts at: 1872 3214 1872
Size: 1342 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2448 3991 4892 5154 820
Size: 197 926 505 1543 901 262 1553
Fragments arranged by size:
1553 1543 926 901 505 262 197
SnaBI TAC'GTA
Cuts at: 493 3678 493
Size: 3185 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5887
SphI G_CATG'C
Cuts at: 1132 3245 1132
Size: 2113 3774
SspI AAT'ATT
Cuts at: 5 52 2683 3811 5
Size: 47 2631 1128 2081
Fragments arranged by size:
2631 2081 1128 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5887
StyI C'CwwG_G
Cuts at: 513 1065 2004 3326 513
Size: 552 939 1322 3074
Fragments arranged by size:
3074 1322 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3062 3914 3931 4084 4269 4714 325
Size: 1255 1482 852 17 153 185 445 1498
Fragments arranged by size:
1498 1482 1255 852 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4133 5845 96
Size: 274 80 33 51 1443 2156 1712 138
Fragments arranged by size:
2156 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2323 2355 2475 2507 5847 5879 2323
Size: 32 120 32 3340 32 2331
Fragments arranged by size:
3340 2331 120 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5887
XbaI T'CTAG_A
Cuts at: 3223 3223
Size: 5887
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5887
XmaI C'CCGG_G
Cuts at: 1870 3212 1870
Size: 1342 4545
XmnI GAAnn'nnTTC
Cuts at: 2640 4016 2640
Size: 1376 4511
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3695 276
Size: 53 83 186 936 2161 2468
Fragments arranged by size:
2468 2161 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoRI EcoRV FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI
PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI
EcoO109I FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI
PsrI RsrII SacI SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI XcmI