Restriction map for
pFN21AB4659
(Circular) (Six-base) MAPSORT of: pFN21AB4659.seq Check: 8026 from: 1 to: 6145
pFN21AB4659 6145 bp
With 182 enzymes: SgfI *
October 20, 2009 10:59 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2840 3955 278
Size: 53 83 186 936 1304 1115 2468
Fragments arranged by size:
2468 1304 1115 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2232 3488 1937
Size: 295 1256 4594
Fragments arranged by size:
4594 1256 295
Acc65I G'GTAC_C
Cuts at: 3466 3466
Size: 6145
AclI AA'CG_TT
Cuts at: 1563 4272 4645 1563
Size: 2709 373 3063
Fragments arranged by size:
3063 2709 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2570 4208 5592 5860 1632
Size: 938 1638 1384 268 1917
Fragments arranged by size:
1917 1638 1384 938 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5948
AflIII A'CryG_T
Cuts at: 1203 5044 6021 1203
Size: 3841 977 1327
Fragments arranged by size:
3841 1327 977
AgeI A'CCGG_T
Cuts at: 4976 4976
Size: 6145
AhdI GACnn_n'nnGTC
Cuts at: 4874 4874
Size: 6145
AleI CACnn'nnGTG
Cuts at: 1166 3150 3164 1166
Size: 1984 14 4147
Fragments arranged by size:
4147 1984 14
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6145
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3086 3118 3086
Size: 32 6113
AlwNI CAG_nnn'CTG
Cuts at: 2197 2283 2334 2596 2806 3091 3253 3888 5460
Size: 86 51 262 210 285 162 635 1572
Cuts at: 5460 2197
Size: 2882
Fragments arranged by size:
2882 1572 635 285 262 210 162 86
51
ApaI G_GGCC'C
Cuts at: 2087 2136 2461 3022 2087
Size: 49 325 561 5210
Fragments arranged by size:
5210 561 325 49
ApaLI G'TGCA_C
Cuts at: 4202 5358 4202
Size: 1156 4989
ApoI r'AATT_y
Cuts at: 1493 3454 3680 3832 5941 1493
Size: 1961 226 152 2109 1697
Fragments arranged by size:
2109 1961 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4699 160
Size: 872 3667 1606
Fragments arranged by size:
3667 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6145
AvaI C'yCGr_G
Cuts at: 1870 1943 2062 2080 2127 2186 2462 3470 1870
Size: 73 119 18 47 59 276 1008 4545
Fragments arranged by size:
4545 1008 276 119 73 59 47 18
BamHI G'GATC_C
Cuts at: 3475 3842 3475
Size: 367 5778
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2286 2337 2692 3001 3466
Size: 325 217 627 499 51 355 309 465
Cuts at: 3466 4921 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 499 465 355 325 309
217 51
BanII G_rGCy'C
Cuts at: 1391 2040 2087 2116 2136 2461 2586 2922 2998
Size: 649 47 29 20 325 125 336 76
Cuts at: 2998 3022 3464 1391
Size: 24 442 4072
Fragments arranged by size:
4072 649 442 336 325 125 76 47
29 24 20
BbeI G_GCGC'C
Cuts at: 2290 2341 2290
Size: 51 6094
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2558 2961 3045 3311 928
Size: 971 659 403 84 266 3762
Fragments arranged by size:
3762 971 659 403 266 84
BbvCI CC'TCA_GC
Cuts at: 2902 2902
Size: 6145
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4336 4370 1903
Size: 34 2399 34 3678
Fragments arranged by size:
3678 2399 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4037 5247 4037
Size: 1210 4935
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6145
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2357 2690 4756
Size: 107 122 71 631 605 685 333 2066
Cuts at: 4756 136
Size: 1525
Fragments arranged by size:
2066 1525 685 631 605 333 122 107
71
BglII A'GATC_T
Cuts at: 6140 6140
Size: 6145
BlpI GC'TnA_GC
Cuts at: 3562 3562
Size: 6145
Bme1580I G_kGCm'C
Cuts at: 1790 2087 2136 2461 3022 4206 5362 1790
Size: 297 49 325 561 1184 1156 2573
Fragments arranged by size:
2573 1184 1156 561 325 297 49
BmgBI CAC'GTC
Cuts at: 1318 2776 1318
Size: 1458 4687
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3876 4834 442
Size: 416 53 83 399 24 2459 958 1753
Fragments arranged by size:
2459 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6145
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2130 3012 2130
Size: 882 5263
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2225 2891 2936 3275 3355 3404 4805 1239
Size: 986 666 45 339 80 49 1401 2579
Fragments arranged by size:
2579 1401 986 666 339 80 49 45
Bpu10I CC'TnA_GC
Cuts at: 2902 2997 2902
Size: 95 6050
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2024 3251 4270 5135 5433 2024
Size: 1227 1019 865 298 2736
Fragments arranged by size:
2736 1227 1019 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4808 882
Size: 617 3309 2219
Fragments arranged by size:
3309 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3367 3936 493
Size: 711 2163 569 2702
Fragments arranged by size:
2702 2163 711 569
BsaBI GATnn'nnATC
Cuts at: 6139 6139
Size: 6145
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2070 2287 2338 2837
Size: 53 83 186 936 537 217 51 499
Cuts at: 2837 3952 4334 4969 275
Size: 1115 382 635 1451
Fragments arranged by size:
1451 1115 936 635 537 499 382 217
186 83 53 51
BsaWI w'CCGG_w
Cuts at: 4578 4976 5250 5397 5808 4578
Size: 398 274 147 411 4915
Fragments arranged by size:
4915 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2609 2639 1178
Size: 30 165 30 1206 30 4684
Fragments arranged by size:
4684 1206 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2108 2171 2617 2861 1186
Size: 922 63 446 244 4470
Fragments arranged by size:
4470 922 446 244 63
BseYI C'CCAG_C
Cuts at: 2135 2443 5348 5844 2135
Size: 308 2905 496 2436
Fragments arranged by size:
2905 2436 496 308
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2243 2276 2291 1163
Size: 573 507 33 15 5017
Fragments arranged by size:
5017 573 507 33 15
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3595 4356 5384 1540
Size: 275 186 1594 761 1028 2301
Fragments arranged by size:
2301 1594 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2040 2565 2586 3171 4206 4291 5362 2040
Size: 525 21 585 1035 85 1071 2823
Fragments arranged by size:
2823 1071 1035 585 525 85 21
BsmI GAATG_Cn'
Cuts at: 3656 3749 3656
Size: 93 6052
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2040 2087 2116 2136 2461 2565 2586
Size: 399 250 47 29 20 325 104 21
Cuts at: 2586 2922 2998 3022 3171 3464 4206 4291 5362
Size: 336 76 24 149 293 742 85 1071
Cuts at: 5362 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 336 325 293 250
149 104 85 76 47 29 24 21
20
BspEI T'CCGG_A
Cuts at: 5808 5808
Size: 6145
BspHI T'CATG_A
Cuts at: 4034 5764 4034
Size: 1730 4415
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2058 2307 3500 844
Size: 1214 249 1193 3489
Fragments arranged by size:
3489 1214 1193 249
BsrBI CCG'CTC
Cuts at: 1394 1823 3259 3404 4032 1394
Size: 429 1436 145 628 3507
Fragments arranged by size:
3507 1436 628 429 145
BsrDI GCAATG_nn'
Cuts at: 65 1248 2346 4640 4814 65
Size: 1183 1098 2294 174 1396
Fragments arranged by size:
2294 1396 1183 1098 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2555 4789 4976 1700
Size: 110 745 2234 187 2869
Fragments arranged by size:
2869 2234 745 187 110
BsrGI T'GTAC_A
Cuts at: 96 3321 96
Size: 3225 2920
BssHII G'CGCG_C
Cuts at: 1925 3389 1925
Size: 1464 4681
BssSI C'ACGA_G
Cuts at: 4205 5217 4205
Size: 1012 5133
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2299 2806 794
Size: 1505 507 4133
Fragments arranged by size:
4133 1505 507
BstBI TT'CG_AA
Cuts at: 4949 4949
Size: 6145
BstEII G'GTnAC_C
Cuts at: 1184 2426 2893 1184
Size: 1242 467 4436
Fragments arranged by size:
4436 1242 467
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2011
Size: 6145
BstYI r'GATC_y
Cuts at: 1972 3475 3842 4227 4244 5696 5782 5794 6140
Size: 1503 367 385 17 1452 86 12 346
Cuts at: 6140 1972
Size: 1977
Fragments arranged by size:
1977 1503 1452 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2658 3075 2658
Size: 417 5728
BtgI C'CryG_G
Cuts at: 513 1065 2151 2513 3043 513
Size: 552 1086 362 530 3615
Fragments arranged by size:
3615 1086 552 530 362
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3121 487
Size: 683 56 1895 3511
Fragments arranged by size:
3511 1895 683 56
BtsI GCAGTG_nn'
Cuts at: 2250 2611 3196 3661 4455 4475 2250
Size: 361 585 465 794 20 3920
Fragments arranged by size:
3920 794 585 465 361 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6145
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6110
DraI TTT'AAA
Cuts at: 3449 3449
Size: 6145
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5152 809
Size: 507 3836 1802
Fragments arranged by size:
3836 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2325 2403 2510
Size: 54 1425 50 275 18 495 78 107
Cuts at: 2510 2577 3592 4481 6110 8
Size: 67 1015 889 1629 43
Fragments arranged by size:
1629 1425 1015 889 495 275 107 78
67 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3592 1537
Size: 275 1780 4090
Fragments arranged by size:
4090 1780 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3335 4075 1353
Size: 1982 740 3423
Fragments arranged by size:
3423 1982 740
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2030 3335 4732 5106 5252 387
Size: 1064 138 441 1305 1397 374 146 1280
Fragments arranged by size:
1397 1305 1280 1064 441 374 146 138
EcoICRI GAG'CTC
Cuts at: 2038 2584 2038
Size: 546 5599
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2225 2570 2891 2936 3275 3355 3404
Size: 393 593 345 321 45 339 80 49
Cuts at: 3404 4208 4805 5592 5860 1239
Size: 804 597 787 268 1524
Fragments arranged by size:
1524 804 787 597 593 393 345 339
321 268 80 49 45
EcoNI CCTnn'n_nnAGG
Cuts at: 2168 2657 2168
Size: 489 5656
EcoO109I rG'GnC_Cy
Cuts at: 2084 2123 2132 2161 2458 2734 2084
Size: 39 9 29 297 276 5495
Fragments arranged by size:
5495 297 276 39 29 9
EcoRI G'AATT_C
Cuts at: 3454 3832 3454
Size: 378 5767
FspI TGC'GCA
Cuts at: 2474 4651 2474
Size: 2177 3968
HaeII r_GCGC'y
Cuts at: 1678 2142 2290 2341 2786 5292 1678
Size: 464 148 51 445 2506 2531
Fragments arranged by size:
2531 2506 464 445 148 51
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2822 2854 4786 4818 4860
Size: 32 135 32 1250 32 1932 32 42
Cuts at: 4860 4892 1373
Size: 32 2626
Fragments arranged by size:
2626 1932 1250 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3489 3735 669
Size: 988 281 1551 246 3079
Fragments arranged by size:
3079 1551 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6145
HpaI GTT'AAC
Cuts at: 3735 3735
Size: 6145
KasI G'GCGC_C
Cuts at: 2286 2337 2286
Size: 51 6094
KpnI G_GTAC'C
Cuts at: 3470 3470
Size: 6145
MfeI C'AATT_G
Cuts at: 3744 3744
Size: 6145
MluI A'CGCG_T
Cuts at: 6021 6021
Size: 6145
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5259 5443 1305
Size: 3954 184 2007
Fragments arranged by size:
3954 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2327 10
Size: 54 1425 343 495 3828
Fragments arranged by size:
3828 1425 495 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2150 2313 3140 3150 3164 4103
Size: 648 843 141 163 827 10 14 939
Cuts at: 4103 4462 4621 518
Size: 359 159 2042
Fragments arranged by size:
2042 939 843 827 648 359 163 159
141 14 10
MspA1I CmG'CkG
Cuts at: 1606 2806 2906 3064 3088 3561 4238 5386 5631
Size: 1200 100 158 24 473 677 1148 245
Cuts at: 5631 1606
Size: 2120
Fragments arranged by size:
2120 1200 1148 677 473 245 158 100
24
NarI GG'CG_CC
Cuts at: 2287 2338 2287
Size: 51 6094
NcoI C'CATG_G
Cuts at: 513 1065 2513 3043 513
Size: 552 1448 530 3615
Fragments arranged by size:
3615 1448 552 530
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6145
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6145
NotI GC'GGCC_GC
Cuts at: 3592 3592
Size: 6145
NspI r_CATG'y
Cuts at: 1132 1229 2800 2941 3397 3503 5048 1132
Size: 97 1571 141 456 106 1545 2229
Fragments arranged by size:
2229 1571 1545 456 141 106 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6145
PciI A'CATG_T
Cuts at: 5044 5044
Size: 6145
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2283 2334 3253 1108
Size: 1175 51 919 4000
Fragments arranged by size:
4000 1175 919 51
PfoI T'CCnGG_A
Cuts at: 2913 3252 3381 2913
Size: 339 129 5677
Fragments arranged by size:
5677 339 129
PmeI GTTT'AAAC
Cuts at: 3449 3449
Size: 6145
PmlI CAC'GTG
Cuts at: 3367 3367
Size: 6145
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4214 4246 5727 5759 4214
Size: 32 1481 32 4600
Fragments arranged by size:
4600 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2161 2734 2161
Size: 573 5572
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6145
PsiI TTA'TAA
Cuts at: 3715 3715
Size: 6145
PspOMI G'GGCC_C
Cuts at: 2083 2132 2457 3018 2083
Size: 49 325 561 5210
Fragments arranged by size:
5210 561 325 49
PspXI vC'TCGA_Gb
Cuts at: 2127 2186 2127
Size: 59 6086
PstI C_TGCA'G
Cuts at: 830 2418 2811 3497 830
Size: 1588 393 686 3478
Fragments arranged by size:
3478 1588 686 393
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6145
PvuII CAG'CTG
Cuts at: 2806 2906 3088 2806
Size: 100 182 5863
Fragments arranged by size:
5863 182 100
SacI G_AGCT'C
Cuts at: 2040 2586 2040
Size: 546 5599
SalI G'TCGA_C
Cuts at: 1936 3487 1936
Size: 1551 4594
SanDI GG'GwC_CC
Cuts at: 2734 2734
Size: 6145
SbfI CC_TGCA'GG
Cuts at: 3497 3497
Size: 6145
ScaI AGT'ACT
Cuts at: 4393 6105 4393
Size: 1712 4433
SexAI A'CCwGG_T
Cuts at: 2385 2385
Size: 6145
SfcI C'TryA_G
Cuts at: 826 1045 2414 2807 3115 3493 4628 5309 5500
Size: 219 1369 393 308 378 1135 681 191
Cuts at: 5500 826
Size: 1471
Fragments arranged by size:
1471 1369 1135 681 393 378 308 219
191
SfoI GGC'GCC
Cuts at: 2288 2339 2288
Size: 51 6094
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6145
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6145
SmaI CCC'GGG
Cuts at: 1872 2064 3472 1872
Size: 192 1408 4545
Fragments arranged by size:
4545 1408 192
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2039 2127 2186 3266 4249 5150
Size: 197 926 96 88 59 1080 983 901
Cuts at: 5150 5412 820
Size: 262 1553
Fragments arranged by size:
1553 1080 983 926 901 262 197 96
88 59
SnaBI TAC'GTA
Cuts at: 493 3936 493
Size: 3443 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6145
SphI G_CATG'C
Cuts at: 1132 2800 3397 3503 1132
Size: 1668 597 106 3774
Fragments arranged by size:
3774 1668 597 106
SspI AAT'ATT
Cuts at: 5 52 4069 5
Size: 47 4017 2081
Fragments arranged by size:
4017 2081 47
StuI AGG'CCT
Cuts at: 1517 2543 1517
Size: 1026 5119
StyI C'CwwG_G
Cuts at: 513 1065 2322 2513 2526 3043 3584 513
Size: 552 1257 191 13 517 541 3074
Fragments arranged by size:
3074 1257 552 541 517 191 13
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2624 2946 4172 4189 4342 4527 4972
Size: 1255 1044 322 1226 17 153 185 445
Cuts at: 4972 325
Size: 1498
Fragments arranged by size:
1498 1255 1226 1044 445 322 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3321 4391 6103
Size: 274 80 33 51 1443 1344 1070 1712
Cuts at: 6103 96
Size: 138
Fragments arranged by size:
1712 1443 1344 1070 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2009 2041 2302 2334 2555 2587 2609 2641 6105
Size: 32 261 32 221 32 22 32 3464
Cuts at: 6105 6137 2009
Size: 32 2017
Fragments arranged by size:
3464 2017 261 221 32 32 32 32
32 22
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6145
XbaI T'CTAG_A
Cuts at: 3481 3481
Size: 6145
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3203 3203
Size: 6145
XhoI C'TCGA_G
Cuts at: 1943 2127 2186 1943
Size: 184 59 5902
Fragments arranged by size:
5902 184 59
XmaI C'CCGG_G
Cuts at: 1870 2062 3470 1870
Size: 192 1408 4545
Fragments arranged by size:
4545 1408 192
XmnI GAAnn'nnTTC
Cuts at: 4274 4274
Size: 6145
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2838 3953 276
Size: 53 83 186 936 1304 1115 2468
Fragments arranged by size:
2468 1304 1115 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NarI NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI
SanDI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BfrBI BsiWI BsmBI
BstZ17I DraIII EcoRV FalI FseI FspAI NaeI NgoMIV
NruI NsiI PacI PsrI RsrII SacII SapI SfiI
SrfI SwaI