Restriction map for
pFN21AB4658
(Circular) (Six-base) MAPSORT of: pFN21AB4658.seq Check: 4373 from: 1 to: 7123
pFN21AB4658 7123 bp
With 182 enzymes: SgfI *
October 20, 2009 10:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3450 3450
Size: 7123
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3735 4933 278
Size: 53 83 186 936 2199 1198 2468
Fragments arranged by size:
2468 2199 1198 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4365 4466 1937
Size: 2428 101 4594
Fragments arranged by size:
4594 2428 101
Acc65I G'GTAC_C
Cuts at: 4444 4444
Size: 7123
AclI AA'CG_TT
Cuts at: 1563 3852 5250 5623 1563
Size: 2289 1398 373 3063
Fragments arranged by size:
3063 2289 1398 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2020 3716 4261 5186 6570 6838 1632
Size: 388 1696 545 925 1384 268 1917
Fragments arranged by size:
1917 1696 1384 925 545 388 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6926
AflIII A'CryG_T
Cuts at: 1203 6022 6999 1203
Size: 4819 977 1327
Fragments arranged by size:
4819 1327 977
AgeI A'CCGG_T
Cuts at: 5954 5954
Size: 7123
AhdI GACnn_n'nnGTC
Cuts at: 5852 5852
Size: 7123
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7123
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2486 3300 1184
Size: 1302 814 5007
Fragments arranged by size:
5007 1302 814
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3632 3664 3632
Size: 32 7091
AlwNI CAG_nnn'CTG
Cuts at: 2308 2332 2608 2636 3824 4027 4411 4866 6438
Size: 24 276 28 1188 203 384 455 1572
Cuts at: 6438 2308
Size: 2993
Fragments arranged by size:
2993 1572 1188 455 384 276 203 28
24
ApaI G_GGCC'C
Cuts at: 2756 3385 3681 2756
Size: 629 296 6198
Fragments arranged by size:
6198 629 296
ApaLI G'TGCA_C
Cuts at: 5180 6336 5180
Size: 1156 5967
ApoI r'AATT_y
Cuts at: 1493 2251 4432 4658 4810 6919 1493
Size: 758 2181 226 152 2109 1697
Fragments arranged by size:
2181 2109 1697 758 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5677 160
Size: 872 4645 1606
Fragments arranged by size:
4645 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7123
AvaI C'yCGr_G
Cuts at: 1870 1943 2104 3514 4448 1870
Size: 73 161 1410 934 4545
Fragments arranged by size:
4545 1410 934 161 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2404 2437 2404
Size: 33 7090
BamHI G'GATC_C
Cuts at: 2394 4213 4453 4820 2394
Size: 1819 240 367 4697
Fragments arranged by size:
4697 1819 367 240
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3554 4444 5899 618
Size: 325 217 627 1767 890 1455 1842
Fragments arranged by size:
1842 1767 1455 890 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2297 2756 3284 3322 3385 3410 3659 3681
Size: 906 459 528 38 63 25 249 22
Cuts at: 3681 4442 1391
Size: 761 4072
Fragments arranged by size:
4072 906 761 528 459 249 63 38
25 22
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2563 4120 928
Size: 971 664 1557 3931
Fragments arranged by size:
3931 1557 971 664
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2974 3008 5314 5348 1903
Size: 34 50 34 953 34 2306 34 3678
Fragments arranged by size:
3678 2306 953 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5015 6225 5015
Size: 1210 5913
BclI T'GATC_A
Cuts at: 1548 2814 3099 1548
Size: 1266 285 5572
Fragments arranged by size:
5572 1266 285
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2378 2760 5734
Size: 107 122 71 631 605 706 382 2974
Cuts at: 5734 136
Size: 1525
Fragments arranged by size:
2974 1525 706 631 605 382 122 107
71
BglII A'GATC_T
Cuts at: 7118 7118
Size: 7123
BlpI GC'TnA_GC
Cuts at: 3321 3873 4540 3321
Size: 552 667 5904
Fragments arranged by size:
5904 667 552
Bme1580I G_kGCm'C
Cuts at: 1790 2756 3385 3557 3681 4243 5184 6340 1790
Size: 966 629 172 124 562 941 1156 2573
Fragments arranged by size:
2573 1156 966 941 629 562 172 124
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7123
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2890 3553 4193
Size: 416 53 83 399 24 1473 663 640
Cuts at: 4193 4854 5812 442
Size: 661 958 1753
Fragments arranged by size:
1753 1473 958 663 661 640 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7123
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2316 3298 3529 2316
Size: 982 231 5910
Fragments arranged by size:
5910 982 231
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2246 3585 3643 3704 3801 3852 5783 1239
Size: 1007 1339 58 61 97 51 1931 2579
Fragments arranged by size:
2579 1931 1339 1007 97 61 58 51
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5248 6113 6411 5248
Size: 865 298 5960
Fragments arranged by size:
5960 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2558 4349 5786 882
Size: 617 1059 1791 1437 2219
Fragments arranged by size:
2219 1791 1437 1059 617
BsaAI yAC'GTr
Cuts at: 493 1204 2971 4914 493
Size: 711 1767 1943 2702
Fragments arranged by size:
2702 1943 1767 711
BsaBI GATnn'nnATC
Cuts at: 2816 7117 2816
Size: 4301 2822
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2043 2241 3732 4930
Size: 53 83 186 936 510 198 1491 1198
Cuts at: 4930 5312 5947 275
Size: 382 635 1451
Fragments arranged by size:
1491 1451 1198 936 635 510 382 198
186 83 53
BsaWI w'CCGG_w
Cuts at: 3801 5556 5954 6228 6375 6786 3801
Size: 1755 398 274 147 411 4138
Fragments arranged by size:
4138 1755 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2553 2583 3114 3144 3450
Size: 30 165 30 1150 30 531 30 306
Cuts at: 3450 3480 1178
Size: 30 4821
Fragments arranged by size:
4821 1150 531 306 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2468 3474 3477 3482 3701 4400 1186
Size: 1282 1006 3 5 219 699 3909
Fragments arranged by size:
3909 1282 1006 699 219 5 3
BseYI C'CCAG_C
Cuts at: 2271 2357 2692 4136 4290 4294 6326 6822 2271
Size: 86 335 1444 154 4 2032 496 2572
Fragments arranged by size:
2572 2032 1444 496 335 154 86 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2294 2446 2456 2540 2660 2951 3050
Size: 573 558 152 10 84 120 291 99
Cuts at: 3050 3575 1163
Size: 525 4711
Fragments arranged by size:
4711 573 558 525 291 152 120 99
84 10
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4573 5334 6362 1540
Size: 275 186 2572 761 1028 2301
Fragments arranged by size:
2572 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2297 3051 3322 3717 4380 5184 5269 6340 2297
Size: 754 271 395 663 804 85 1071 3080
Fragments arranged by size:
3080 1071 804 754 663 395 271 85
BsmI GAATG_Cn'
Cuts at: 4634 4727 4634
Size: 93 7030
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2297 2756 3051 3284 3322 3385 3410
Size: 399 507 459 295 233 38 63 25
Cuts at: 3410 3557 3659 3681 3717 4243 4380 4442 5184
Size: 147 102 22 36 526 137 62 742
Cuts at: 5184 5269 6340 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 526 507 459 399 295
233 147 137 102 85 63 62 38
36 25 22
BspEI T'CCGG_A
Cuts at: 3801 6786 3801
Size: 2985 4138
BspHI T'CATG_A
Cuts at: 5012 6742 5012
Size: 1730 5393
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3450 4478 844
Size: 2606 1028 3489
Fragments arranged by size:
3489 2606 1028
BsrBI CCG'CTC
Cuts at: 1394 1823 5010 1394
Size: 429 3187 3507
Fragments arranged by size:
3507 3187 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2737 5618 5792 65
Size: 1183 1489 2881 174 1396
Fragments arranged by size:
2881 1489 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3774 3781 5767 5954 1700
Size: 110 1964 7 1986 187 2869
Fragments arranged by size:
2869 1986 1964 187 110 7
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7123
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7123
BssSI C'ACGA_G
Cuts at: 5183 6195 5183
Size: 1012 6111
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7123
BstBI TT'CG_AA
Cuts at: 5927 5927
Size: 7123
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7123
BstXI CCAn_nnnn'nTGG
Cuts at: 4183 4183
Size: 7123
BstYI r'GATC_y
Cuts at: 1972 2394 2502 2838 3361 4005 4213 4453 4820
Size: 422 108 336 523 644 208 240 367
Cuts at: 4820 5205 5222 6674 6760 6772 7118 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 644 523 422 385 367 346
336 240 208 108 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2301 3860 2301
Size: 1559 5564
BtgI C'CryG_G
Cuts at: 513 1065 2949 3019 3040 3228 3259 3270 3603
Size: 552 1884 70 21 188 31 11 333
Cuts at: 3603 513
Size: 4033
Fragments arranged by size:
4033 1884 552 333 188 70 31 21
11
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3481 487
Size: 683 56 2255 4129
Fragments arranged by size:
4129 2255 683 56
BtsI GCAGTG_nn'
Cuts at: 2637 4639 5433 5453 2637
Size: 2002 794 20 4307
Fragments arranged by size:
4307 2002 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7123
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7088
DraI TTT'AAA
Cuts at: 4427 4427
Size: 7123
DraIII CAC_nnn'GTG
Cuts at: 2204 2204
Size: 7123
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4093 6130 809
Size: 507 2777 2037 1802
Fragments arranged by size:
2777 2037 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2017 2091 2778
Size: 54 1425 50 275 18 187 74 687
Cuts at: 2778 3072 3522 3582 3972 4155 4570 5459 7088
Size: 294 450 60 390 183 415 889 1629
Cuts at: 7088 8
Size: 43
Fragments arranged by size:
1629 1425 889 687 450 415 390 294
275 187 183 74 60 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4570 1537
Size: 275 2758 4090
Fragments arranged by size:
4090 2758 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2222 3803 5053 1353
Size: 869 1581 1250 3423
Fragments arranged by size:
3423 1581 1250 869
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5710 6084 6230 387
Size: 1064 138 4121 374 146 1280
Fragments arranged by size:
4121 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2295 3320 2295
Size: 1025 6098
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2020 2246 3585 3643 3704 3716 3801
Size: 393 388 226 1339 58 61 12 85
Cuts at: 3801 3852 4261 5186 5783 6570 6838 1239
Size: 51 409 925 597 787 268 1524
Fragments arranged by size:
1524 1339 925 787 597 409 393 388
268 226 85 61 58 51 12
EcoNI CCTnn'n_nnAGG
Cuts at: 2645 2645
Size: 7123
EcoO109I rG'GnC_Cy
Cuts at: 2305 2320 2753 3106 3265 3532 3663 3677 3678
Size: 15 433 353 159 267 131 14 1
Cuts at: 3678 4024 4095 2305
Size: 346 71 5333
Fragments arranged by size:
5333 433 353 346 267 159 131 71
15 14 1
EcoRI G'AATT_C
Cuts at: 4432 4810 4432
Size: 378 6745
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3226 3226
Size: 7123
FspI TGC'GCA
Cuts at: 2984 5629 2984
Size: 2645 4478
HaeII r_GCGC'y
Cuts at: 1678 3293 3952 6270 1678
Size: 1615 659 2318 2531
Fragments arranged by size:
2531 2318 1615 659
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2087 2119 2199 2231 2553
Size: 32 135 32 515 32 80 32 322
Cuts at: 2553 2585 3199 3231 5764 5796 5838 5870 1373
Size: 32 614 32 2533 32 42 32 2626
Fragments arranged by size:
2626 2533 614 515 322 135 80 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2185 3856 4342 4467 4713 669
Size: 988 281 247 1671 486 125 246 3079
Fragments arranged by size:
3079 1671 988 486 281 247 246 125
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7123
HpaI GTT'AAC
Cuts at: 3856 4713 3856
Size: 857 6266
KpnI G_GTAC'C
Cuts at: 4448 4448
Size: 7123
MfeI C'AATT_G
Cuts at: 2437 4722 2437
Size: 2285 4838
MluI A'CGCG_T
Cuts at: 6999 6999
Size: 7123
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2211 2236 3961 6237 6421 1305
Size: 906 25 1725 2276 184 2007
Fragments arranged by size:
2276 2007 1725 906 184 25
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3074 3524 3584 3974 4157
Size: 54 1425 343 1242 450 60 390 183
Cuts at: 4157 10
Size: 2976
Fragments arranged by size:
2976 1425 1242 450 390 343 183 60
54
MslI CAynn'nnrTG
Cuts at: 518 1166 2537 2822 3722 5081 5440 5599 518
Size: 648 1371 285 900 1359 359 159 2042
Fragments arranged by size:
2042 1371 1359 900 648 359 285 159
MspA1I CmG'CkG
Cuts at: 1606 2134 2224 2275 2361 2430 2674 2692 2951
Size: 528 90 51 86 69 244 18 259
Cuts at: 2951 3042 3064 3272 3889 3915 4294 4408 4539
Size: 91 22 208 617 26 379 114 131
Cuts at: 4539 5216 6364 6609 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 617 528 379 259 245
244 208 131 114 91 90 86 69
51 26 22 18
NaeI GCC'GGC
Cuts at: 3783 3783
Size: 7123
NcoI C'CATG_G
Cuts at: 513 1065 3603 513
Size: 552 2538 4033
Fragments arranged by size:
4033 2538 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7123
NgoMIV G'CCGG_C
Cuts at: 3781 3781
Size: 7123
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7123
NotI GC'GGCC_GC
Cuts at: 4570 4570
Size: 7123
NspI r_CATG'y
Cuts at: 1132 1229 2911 3616 3625 4481 6026 1132
Size: 97 1682 705 9 856 1545 2229
Fragments arranged by size:
2229 1682 1545 856 705 97 9
PasI CC'CwG_GG
Cuts at: 1348 4081 4146 1348
Size: 2733 65 4325
Fragments arranged by size:
4325 2733 65
PciI A'CATG_T
Cuts at: 6022 6022
Size: 7123
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7123
PfoI T'CCnGG_A
Cuts at: 3828 3954 3828
Size: 126 6997
PmeI GTTT'AAAC
Cuts at: 4427 4427
Size: 7123
PmlI CAC'GTG
Cuts at: 2971 2971
Size: 7123
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3780 3812 5192 5224 6705 6737 3780
Size: 32 1380 32 1481 32 4166
Fragments arranged by size:
4166 1481 1380 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2305 2320 3265 3532 4024 4095 2305
Size: 15 945 267 492 71 5333
Fragments arranged by size:
5333 945 492 267 71 15
PshAI GACnn'nnGTC
Cuts at: 1613 3731 4361 1613
Size: 2118 630 4375
Fragments arranged by size:
4375 2118 630
PsiI TTA'TAA
Cuts at: 4693 4693
Size: 7123
PspOMI G'GGCC_C
Cuts at: 2752 3381 3677 2752
Size: 629 296 6198
Fragments arranged by size:
6198 629 296
PspXI vC'TCGA_Gb
Cuts at: 2104 2104
Size: 7123
PstI C_TGCA'G
Cuts at: 830 2328 2613 3289 4475 830
Size: 1498 285 676 1186 3478
Fragments arranged by size:
3478 1498 1186 676 285
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7123
PvuII CAG'CTG
Cuts at: 2134 2224 2275 2361 2430 2692 3889 4294 4408
Size: 90 51 86 69 262 1197 405 114
Cuts at: 4408 2134
Size: 4849
Fragments arranged by size:
4849 1197 405 262 114 90 86 69
51
SacI G_AGCT'C
Cuts at: 2297 3322 2297
Size: 1025 6098
SacII CC_GC'GG
Cuts at: 2952 3043 3273 2952
Size: 91 230 6802
Fragments arranged by size:
6802 230 91
SalI G'TCGA_C
Cuts at: 1936 4465 1936
Size: 2529 4594
SanDI GG'GwC_CC
Cuts at: 3265 3265
Size: 7123
SbfI CC_TGCA'GG
Cuts at: 4475 4475
Size: 7123
ScaI AGT'ACT
Cuts at: 2525 4274 5371 7083 2525
Size: 1749 1097 1712 2565
Fragments arranged by size:
2565 1749 1712 1097
SexAI A'CCwGG_T
Cuts at: 2646 2646
Size: 7123
SfcI C'TryA_G
Cuts at: 826 1045 2324 2609 3285 3356 4471 5606 6287
Size: 219 1279 285 676 71 1115 1135 681
Cuts at: 6287 6478 826
Size: 191 1471
Fragments arranged by size:
1471 1279 1135 1115 681 676 285 219
191 71
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7123
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7123
SmaI CCC'GGG
Cuts at: 1872 4450 1872
Size: 2578 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2104 5227 6128 6390 820
Size: 197 926 161 3123 901 262 1553
Fragments arranged by size:
3123 1553 926 901 262 197 161
SnaBI TAC'GTA
Cuts at: 493 4914 493
Size: 4421 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7123
SphI G_CATG'C
Cuts at: 1132 2911 4481 1132
Size: 1779 1570 3774
Fragments arranged by size:
3774 1779 1570
SspI AAT'ATT
Cuts at: 5 52 5047 5
Size: 47 4995 2081
Fragments arranged by size:
4995 2081 47
StuI AGG'CCT
Cuts at: 1517 3474 3512 3865 1517
Size: 1957 38 353 4775
Fragments arranged by size:
4775 1957 353 38
StyI C'CwwG_G
Cuts at: 513 1065 2617 2659 3528 3603 3744 4176 4562
Size: 552 1552 42 869 75 141 432 386
Cuts at: 4562 513
Size: 3074
Fragments arranged by size:
3074 1552 869 552 432 386 141 75
42
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2127 2351 3268 3535 4016 4373 5150
Size: 1255 547 224 917 267 481 357 777
Cuts at: 5150 5167 5320 5505 5950 325
Size: 17 153 185 445 1498
Fragments arranged by size:
1498 1255 917 777 547 481 445 357
267 224 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2523 3624 4014
Size: 274 80 33 51 1443 546 1101 390
Cuts at: 4014 4272 5369 7081 96
Size: 258 1097 1712 138
Fragments arranged by size:
1712 1443 1101 1097 546 390 274 258
138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3114 3146 3450 3482 3584 3616 7083 7115 3114
Size: 32 304 32 102 32 3467 32 3122
Fragments arranged by size:
3467 3122 304 102 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2174 3766 1316
Size: 858 1592 4673
Fragments arranged by size:
4673 1592 858
XbaI T'CTAG_A
Cuts at: 4459 4459
Size: 7123
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2365 2365
Size: 7123
XhoI C'TCGA_G
Cuts at: 1943 2104 1943
Size: 161 6962
XmaI C'CCGG_G
Cuts at: 1870 4448 1870
Size: 2578 4545
XmnI GAAnn'nnTTC
Cuts at: 5252 5252
Size: 7123
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3733 4931 276
Size: 53 83 186 936 2199 1198 2468
Fragments arranged by size:
2468 2199 1198 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI
SacII SalI SanDI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BbeI BbvCI BfrBI Bpu10I BsiWI
BsmBI BstZ17I EcoRV FseI FspAI KasI NarI NruI
NsiI PacI PsrI RsrII SapI SfiI SfoI SrfI
SwaI