Restriction map for
pFN21AB4629
(Circular) (Six-base) MAPSORT of: pFN21AB4629.seq Check: 3310 from: 1 to: 6028
pFN21AB4629 6028 bp
With 182 enzymes: SgfI *
October 20, 2009 10:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3165 3165
Size: 6028
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3838 278
Size: 53 83 186 936 2302 2468
Fragments arranged by size:
2468 2302 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3371 1937
Size: 1434 4594
Acc65I G'GTAC_C
Cuts at: 3349 3349
Size: 6028
AclI AA'CG_TT
Cuts at: 1563 3102 4155 4528 1563
Size: 1539 1053 373 3063
Fragments arranged by size:
3063 1539 1053 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2549 2797 2959 4091 5475 5743 1632
Size: 917 248 162 1132 1384 268 1917
Fragments arranged by size:
1917 1384 1132 917 268 248 162
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5831
AflIII A'CryG_T
Cuts at: 1203 3321 4927 5904 1203
Size: 2118 1606 977 1327
Fragments arranged by size:
2118 1606 1327 977
AgeI A'CCGG_T
Cuts at: 4859 4859
Size: 6028
AhdI GACnn_n'nnGTC
Cuts at: 4757 4757
Size: 6028
AleI CACnn'nnGTG
Cuts at: 1166 2262 2762 1166
Size: 1096 500 4432
Fragments arranged by size:
4432 1096 500
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6028
AlwNI CAG_nnn'CTG
Cuts at: 3771 5343 3771
Size: 1572 4456
ApaLI G'TGCA_C
Cuts at: 2732 2755 2861 4085 5241 2732
Size: 23 106 1224 1156 3519
Fragments arranged by size:
3519 1224 1156 106 23
ApoI r'AATT_y
Cuts at: 1493 3002 3149 3337 3563 3715 5824 1493
Size: 1509 147 188 226 152 2109 1697
Fragments arranged by size:
2109 1697 1509 226 188 152 147
AseI AT'TA_AT
Cuts at: 160 1032 4582 160
Size: 872 3550 1606
Fragments arranged by size:
3550 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6028
AvaI C'yCGr_G
Cuts at: 1870 1943 3353 1870
Size: 73 1410 4545
Fragments arranged by size:
4545 1410 73
BamHI G'GATC_C
Cuts at: 3358 3725 3358
Size: 367 5661
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2629 3349 4804 618
Size: 325 217 627 842 720 1455 1842
Fragments arranged by size:
1842 1455 842 720 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2083 2091 2150 2784 3347 1391
Size: 692 8 59 634 563 4072
Fragments arranged by size:
4072 692 634 563 59 8
BbeI G_GCGC'C
Cuts at: 2633 2633
Size: 6028
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2731 3275 928
Size: 971 832 544 3681
Fragments arranged by size:
3681 971 832 544
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4219 4253 1903
Size: 34 2282 34 3678
Fragments arranged by size:
3678 2282 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3920 5130 3920
Size: 1210 4818
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6028
BfrBI ATG'CAT
Cuts at: 2273 2273
Size: 6028
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2885 4639 136
Size: 107 122 71 631 605 1213 1754 1525
Fragments arranged by size:
1754 1525 1213 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3309 6023 3309
Size: 2714 3314
BlpI GC'TnA_GC
Cuts at: 3445 3445
Size: 6028
Bme1580I G_kGCm'C
Cuts at: 1790 2736 2759 2865 3145 4089 5245 1790
Size: 946 23 106 280 944 1156 2573
Fragments arranged by size:
2573 1156 946 944 280 106 23
BmgBI CAC'GTC
Cuts at: 1318 2737 1318
Size: 1419 4609
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2420 3175 3759
Size: 416 53 83 399 24 1003 755 584
Cuts at: 3759 4717 442
Size: 958 1753
Fragments arranged by size:
1753 1003 958 755 584 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6028
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2342 3287 4688 1239
Size: 1103 945 1401 2579
Fragments arranged by size:
2579 1401 1103 945
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2834 4153 5018 5316 2834
Size: 1319 865 298 3546
Fragments arranged by size:
3546 1319 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2762 3075 3275 4691 882
Size: 617 1263 313 200 1416 2219
Fragments arranged by size:
2219 1416 1263 617 313 200
BsaAI yAC'GTr
Cuts at: 493 1204 3819 493
Size: 711 2615 2702
Fragments arranged by size:
2702 2615 711
BsaBI GATnn'nnATC
Cuts at: 6022 6022
Size: 6028
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2630 3835 4217 4852
Size: 53 83 186 936 1097 1205 382 635
Cuts at: 4852 275
Size: 1451
Fragments arranged by size:
1451 1205 1097 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4461 4859 5133 5280 5691 4461
Size: 398 274 147 411 4798
Fragments arranged by size:
4798 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5803
Fragments arranged by size:
5803 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2060 2063 2140 1186
Size: 874 3 77 5074
Fragments arranged by size:
5074 874 77 3
BseYI C'CCAG_C
Cuts at: 2807 5231 5727 2807
Size: 2424 496 3108
Fragments arranged by size:
3108 2424 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3275 1163
Size: 573 1539 3916
Fragments arranged by size:
3916 1539 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3478 4239 5267 1540
Size: 275 186 1477 761 1028 2301
Fragments arranged by size:
2301 1477 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2091 2150 2736 2759 2784 2865 3243 4089 4174
Size: 59 586 23 25 81 378 846 85
Cuts at: 4174 5245 2091
Size: 1071 2874
Fragments arranged by size:
2874 1071 846 586 378 85 81 59
25 23
BsiWI C'GTAC_G
Cuts at: 2303 2303
Size: 6028
BsmI GAATG_Cn'
Cuts at: 3539 3632 3539
Size: 93 5935
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2083 2091 2150 2736 2759 2784 2865
Size: 399 293 8 59 586 23 25 81
Cuts at: 2865 3145 3243 3347 4089 4174 5245 1391
Size: 280 98 104 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 586 399 293 280 104
98 85 81 59 25 23 8
BspEI T'CCGG_A
Cuts at: 5691 5691
Size: 6028
BspHI T'CATG_A
Cuts at: 3096 3917 5647 3096
Size: 821 1730 3477
Fragments arranged by size:
3477 1730 821
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3165 3228 3383 844
Size: 2321 63 155 3489
Fragments arranged by size:
3489 2321 155 63
BsrBI CCG'CTC
Cuts at: 1394 1823 3915 1394
Size: 429 2092 3507
Fragments arranged by size:
3507 2092 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3057 4523 4697 65
Size: 1183 1809 1466 174 1396
Fragments arranged by size:
1809 1466 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2609 3084 4672 4859 1700
Size: 110 799 475 1588 187 2869
Fragments arranged by size:
2869 1588 799 475 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6028
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6028
BssSI C'ACGA_G
Cuts at: 2864 4088 5100 2864
Size: 1224 1012 3792
Fragments arranged by size:
3792 1224 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2376 3298 794
Size: 1582 922 3524
Fragments arranged by size:
3524 1582 922
BstBI TT'CG_AA
Cuts at: 4832 4832
Size: 6028
BstEII G'GTnAC_C
Cuts at: 1184 2839 3232 1184
Size: 1655 393 3980
Fragments arranged by size:
3980 1655 393
BstYI r'GATC_y
Cuts at: 1972 3309 3358 3725 4110 4127 5579 5665 5677
Size: 1337 49 367 385 17 1452 86 12
Cuts at: 5677 6023 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1337 385 367 346 86 49
17 12
Bsu36I CC'TnA_GG
Cuts at: 2031 2031
Size: 6028
BtgI C'CryG_G
Cuts at: 513 1065 2004 3107 513
Size: 552 939 1103 3434
Fragments arranged by size:
3434 1103 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5289
Fragments arranged by size:
5289 683 56
BtsI GCAGTG_nn'
Cuts at: 2162 2935 3544 4338 4358 2162
Size: 773 609 794 20 3832
Fragments arranged by size:
3832 794 773 609 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6028
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2911 2946 529
Size: 35 2347 35 3611
Fragments arranged by size:
3611 2347 35 35
DraI TTT'AAA
Cuts at: 3332 3332
Size: 6028
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5035 809
Size: 507 3719 1802
Fragments arranged by size:
3719 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2520 3063 3086
Size: 54 1425 50 275 18 690 543 23
Cuts at: 3086 3475 4364 5993 8
Size: 389 889 1629 43
Fragments arranged by size:
1629 1425 889 690 543 389 275 54
50 43 23 18
EagI C'GGCC_G
Cuts at: 1537 1812 3475 1537
Size: 275 1663 4090
Fragments arranged by size:
4090 1663 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2037 2456 2828 3194 3958 1353
Size: 684 419 372 366 764 3423
Fragments arranged by size:
3423 764 684 419 372 366
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4615 4989 5135 387
Size: 1064 138 3026 374 146 1280
Fragments arranged by size:
3026 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2089 2148 2782 2089
Size: 59 634 5335
Fragments arranged by size:
5335 634 59
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2342 2549 2797 2959 3287 4091 4688
Size: 393 710 207 248 162 328 804 597
Cuts at: 4688 5475 5743 1239
Size: 787 268 1524
Fragments arranged by size:
1524 804 787 710 597 393 328 268
248 207 162
EcoO109I rG'GnC_Cy
Cuts at: 2196 2196
Size: 6028
EcoRI G'AATT_C
Cuts at: 3149 3337 3715 3149
Size: 188 378 5462
Fragments arranged by size:
5462 378 188
EcoRV GAT'ATC
Cuts at: 2983 2983
Size: 6028
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2311 2311
Size: 6028
FspI TGC'GCA
Cuts at: 2957 4534 2957
Size: 1577 4451
HaeII r_GCGC'y
Cuts at: 1678 2633 5175 1678
Size: 955 2542 2531
Fragments arranged by size:
2542 2531 955
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2183 2215 2231 2263 4669
Size: 32 135 32 611 32 16 32 2406
Cuts at: 4669 4701 4743 4775 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2406 611 135 42 32 32 32
32 32 32 16
HincII GTy'rAC
Cuts at: 669 1657 1938 2899 3372 3618 669
Size: 988 281 961 473 246 3079
Fragments arranged by size:
3079 988 961 473 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6028
HpaI GTT'AAC
Cuts at: 3618 3618
Size: 6028
KasI G'GCGC_C
Cuts at: 2629 2629
Size: 6028
KpnI G_GTAC'C
Cuts at: 3353 3353
Size: 6028
MfeI C'AATT_G
Cuts at: 3627 3627
Size: 6028
MluI A'CGCG_T
Cuts at: 5904 5904
Size: 6028
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2423 5142 5326 1305
Size: 1118 2719 184 2007
Fragments arranged by size:
2719 2007 1118 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4206
Fragments arranged by size:
4206 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2262 2274 2762 3174 3986 4345 4504
Size: 648 1096 12 488 412 812 359 159
Cuts at: 4504 518
Size: 2042
Fragments arranged by size:
2042 1096 812 648 488 412 359 159
12
MspA1I CmG'CkG
Cuts at: 1606 2346 2748 3259 3444 4121 5269 5514 1606
Size: 740 402 511 185 677 1148 245 2120
Fragments arranged by size:
2120 1148 740 677 511 402 245 185
NarI GG'CG_CC
Cuts at: 2630 2630
Size: 6028
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4537
Fragments arranged by size:
4537 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6028
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6028
NotI GC'GGCC_GC
Cuts at: 3475 3475
Size: 6028
NsiI A_TGCA'T
Cuts at: 2275 2275
Size: 6028
NspI r_CATG'y
Cuts at: 1132 1229 2111 2277 2419 3325 3386 4931 1132
Size: 97 882 166 142 906 61 1545 2229
Fragments arranged by size:
2229 1545 906 882 166 142 97 61
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6028
PciI A'CATG_T
Cuts at: 3321 4927 3321
Size: 1606 4422
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6028
PfoI T'CCnGG_A
Cuts at: 2037 2141 3123 3303 2037
Size: 104 982 180 4762
Fragments arranged by size:
4762 982 180 104
PmeI GTTT'AAAC
Cuts at: 3332 3332
Size: 6028
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4097 4129 5610 5642 4097
Size: 32 1481 32 4483
Fragments arranged by size:
4483 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6028
PsiI TTA'TAA
Cuts at: 3598 3598
Size: 6028
PstI C_TGCA'G
Cuts at: 830 2181 3162 3207 3380 830
Size: 1351 981 45 173 3478
Fragments arranged by size:
3478 1351 981 173 45
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6028
PvuII CAG'CTG
Cuts at: 2346 2346
Size: 6028
SacI G_AGCT'C
Cuts at: 2091 2150 2784 2091
Size: 59 634 5335
Fragments arranged by size:
5335 634 59
SalI G'TCGA_C
Cuts at: 1936 3370 1936
Size: 1434 4594
SbfI CC_TGCA'GG
Cuts at: 3162 3380 3162
Size: 218 5810
ScaI AGT'ACT
Cuts at: 2384 4276 5988 2384
Size: 1892 1712 2424
Fragments arranged by size:
2424 1892 1712
SexAI A'CCwGG_T
Cuts at: 2835 2835
Size: 6028
SfcI C'TryA_G
Cuts at: 826 1045 2177 3158 3203 3376 4511 5192 5383
Size: 219 1132 981 45 173 1135 681 191
Cuts at: 5383 826
Size: 1471
Fragments arranged by size:
1471 1135 1132 981 681 219 191 173
45
SfoI GGC'GCC
Cuts at: 2631 2631
Size: 6028
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6028
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6028
SmaI CCC'GGG
Cuts at: 1872 3355 1872
Size: 1483 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2849 4132 5033 5295 820
Size: 197 926 906 1283 901 262 1553
Fragments arranged by size:
1553 1283 926 906 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3819 493
Size: 3326 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6028
SphI G_CATG'C
Cuts at: 1132 2111 3386 1132
Size: 979 1275 3774
Fragments arranged by size:
3774 1275 979
SspI AAT'ATT
Cuts at: 5 52 3952 5
Size: 47 3900 2081
Fragments arranged by size:
3900 2081 47
StuI AGG'CCT
Cuts at: 1517 2534 3093 1517
Size: 1017 559 4452
Fragments arranged by size:
4452 1017 559
StyI C'CwwG_G
Cuts at: 513 1065 2004 3467 513
Size: 552 939 1463 3074
Fragments arranged by size:
3074 1463 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3287 4055 4072 4225 4410 4855 325
Size: 1255 1707 768 17 153 185 445 1498
Fragments arranged by size:
1707 1498 1255 768 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2382 2505 4274
Size: 274 80 33 51 1443 405 123 1769
Cuts at: 4274 5986 96
Size: 1712 138
Fragments arranged by size:
1769 1712 1443 405 274 138 123 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5988 6020 5988
Size: 32 5996
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6028
XbaI T'CTAG_A
Cuts at: 3364 3364
Size: 6028
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6028
XmaI C'CCGG_G
Cuts at: 1870 3353 1870
Size: 1483 4545
XmnI GAAnn'nnTTC
Cuts at: 2534 2570 4157 2534
Size: 36 1587 4405
Fragments arranged by size:
4405 1587 36
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3836 276
Size: 53 83 186 936 2302 2468
Fragments arranged by size:
2468 2302 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NarI
NcoI NdeI NheI NotI NsiI NspI PasI PciI
PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI
PvuII SacI SalI SbfI ScaI SexAI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbvCI BplI
Bpu10I BsmBI BstXI BstZ17I DraIII EcoNI FseI FspAI
NaeI NgoMIV NruI PacI PmlI PpuMI PspOMI PspXI
PsrI RsrII SacII SanDI SapI SfiI SrfI SwaI
XcmI