Restriction map for
pFN21AB4626
(Circular) (Six-base) MAPSORT of: pFN21AB4626.seq Check: 5532 from: 1 to: 7015
pFN21AB4626 7015 bp
With 182 enzymes: SgfI *
February 27, 2009 14:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4228 4228
Size: 7015
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4825 278
Size: 53 83 186 936 3289 2468
Fragments arranged by size:
3289 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4358 1937
Size: 2421 4594
Acc65I G'GTAC_C
Cuts at: 4336 4336
Size: 7015
AclI AA'CG_TT
Cuts at: 1563 5142 5515 1563
Size: 3579 373 3063
Fragments arranged by size:
3579 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3338 3599 5078 6462 6730 1632
Size: 1706 261 1479 1384 268 1917
Fragments arranged by size:
1917 1706 1479 1384 268 261
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6818
AflIII A'CryG_T
Cuts at: 1203 2447 2877 5914 6891 1203
Size: 1244 430 3037 977 1327
Fragments arranged by size:
3037 1327 1244 977 430
AgeI A'CCGG_T
Cuts at: 5846 5846
Size: 7015
AhdI GACnn_n'nnGTC
Cuts at: 5744 5744
Size: 7015
AleI CACnn'nnGTG
Cuts at: 1166 2945 3804 1166
Size: 1779 859 4377
Fragments arranged by size:
4377 1779 859
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2102 2220 2541 2981 3696 1184
Size: 918 118 321 440 715 4503
Fragments arranged by size:
4503 918 715 440 321 118
AlwNI CAG_nnn'CTG
Cuts at: 2169 2344 2545 4006 4228 4758 6330 2169
Size: 175 201 1461 222 530 1572 2854
Fragments arranged by size:
2854 1572 1461 530 222 201 175
ApaI G_GGCC'C
Cuts at: 3256 3596 3256
Size: 340 6675
ApaLI G'TGCA_C
Cuts at: 2114 2195 2354 3671 5072 6228 2114
Size: 81 159 1317 1401 1156 2901
Fragments arranged by size:
2901 1401 1317 1156 159 81
ApoI r'AATT_y
Cuts at: 1493 4324 4550 4702 6811 1493
Size: 2831 226 152 2109 1697
Fragments arranged by size:
2831 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5569 160
Size: 872 4537 1606
Fragments arranged by size:
4537 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7015
AvaI C'yCGr_G
Cuts at: 1870 1943 2419 3042 3131 4299 4340 1870
Size: 73 476 623 89 1168 41 4545
Fragments arranged by size:
4545 1168 623 476 89 73 41
BamHI G'GATC_C
Cuts at: 3696 4345 4712 3696
Size: 649 367 5999
Fragments arranged by size:
5999 649 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2471 2504 2637 3233 3463
Size: 325 217 627 684 33 133 596 230
Cuts at: 3463 3511 4205 4336 5791 618
Size: 48 694 131 1455 1842
Fragments arranged by size:
1842 1455 694 684 627 596 325 230
217 133 131 48 33
BanII G_rGCy'C
Cuts at: 1391 2682 3219 3256 3393 3596 3612 4133 4203
Size: 1291 537 37 137 203 16 521 70
Cuts at: 4203 4334 1391
Size: 131 4072
Fragments arranged by size:
4072 1291 537 521 203 137 131 70
37 16
BbeI G_GCGC'C
Cuts at: 3237 3515 4209 3237
Size: 278 694 6043
Fragments arranged by size:
6043 694 278
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3903 3980 4190 928
Size: 971 2004 77 210 3753
Fragments arranged by size:
3753 2004 971 210 77
BbvCI CC'TCA_GC
Cuts at: 2402 3718 2402
Size: 1316 5699
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5206 5240 1903
Size: 34 3269 34 3678
Fragments arranged by size:
3678 3269 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4907 6117 4907
Size: 1210 5805
BclI T'GATC_A
Cuts at: 1548 3060 1548
Size: 1512 5503
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2630 3457 5626
Size: 107 122 71 631 605 958 827 2169
Cuts at: 5626 136
Size: 1525
Fragments arranged by size:
2169 1525 958 827 631 605 122 107
71
BglII A'GATC_T
Cuts at: 2765 7010 2765
Size: 4245 2770
BlpI GC'TnA_GC
Cuts at: 2843 4432 2843
Size: 1589 5426
Bme1580I G_kGCm'C
Cuts at: 1790 2118 2199 2358 2476 2507 2640 2970 3256
Size: 328 81 159 118 31 133 330 286
Cuts at: 3256 3468 3596 3675 3729 4050 4194 5076 6232
Size: 212 128 79 54 321 144 882 1156
Cuts at: 6232 1790
Size: 2573
Fragments arranged by size:
2573 1156 882 330 328 321 286 212
159 144 133 128 118 81 79 54
31
BmgBI CAC'GTC
Cuts at: 1318 2878 3676 1318
Size: 1560 798 4657
Fragments arranged by size:
4657 1560 798
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3477 4205 4746
Size: 416 53 83 399 24 2060 728 541
Cuts at: 4746 5704 442
Size: 958 1753
Fragments arranged by size:
2060 1753 958 728 541 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7015
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4105 4105
Size: 7015
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2462 3332 3438 4136 5675 1239
Size: 1223 870 106 698 1539 2579
Fragments arranged by size:
2579 1539 1223 870 698 106
Bpu10I CC'TnA_GC
Cuts at: 2402 3110 3380 3718 2402
Size: 708 270 338 5699
Fragments arranged by size:
5699 708 338 270
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5140 6005 6303 5140
Size: 865 298 5852
Fragments arranged by size:
5852 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3924 5678 882
Size: 617 2425 1754 2219
Fragments arranged by size:
2425 2219 1754 617
BsaAI yAC'GTr
Cuts at: 493 1204 2521 4806 493
Size: 711 1317 2285 2702
Fragments arranged by size:
2702 2285 1317 711
BsaBI GATnn'nnATC
Cuts at: 7009 7009
Size: 7015
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2131 3234 3512 3657
Size: 53 83 186 936 598 1103 278 145
Cuts at: 3657 4206 4822 5204 5839 275
Size: 549 616 382 635 1451
Fragments arranged by size:
1451 1103 936 635 616 598 549 382
278 186 145 83 53
BsaWI w'CCGG_w
Cuts at: 2118 3393 3693 3699 5448 5846 6120 6267 6678
Size: 1275 300 6 1749 398 274 147 411
Cuts at: 6678 2118
Size: 2455
Fragments arranged by size:
2455 1749 1275 411 398 300 274 147
6
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2570 2600 3139 3169 3462
Size: 30 165 30 1167 30 539 30 293
Cuts at: 3462 3492 1178
Size: 30 4701
Fragments arranged by size:
4701 1167 539 293 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3259 3730 1186
Size: 2073 471 4471
Fragments arranged by size:
4471 2073 471
BseYI C'CCAG_C
Cuts at: 2064 3853 3883 3950 6218 6714 2064
Size: 1789 30 67 2268 496 2365
Fragments arranged by size:
2365 2268 1789 496 67 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2282 2501 3371 1163
Size: 573 546 219 870 4807
Fragments arranged by size:
4807 870 573 546 219
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2272 4465 5226 6254 1540
Size: 275 186 271 2193 761 1028 2301
Fragments arranged by size:
2301 2193 1028 761 275 271 186
BsiHKAI G_wGCw'C
Cuts at: 2118 2199 2238 2358 2682 3393 3527 3612 3675
Size: 81 39 120 324 711 134 85 63
Cuts at: 3675 4133 5076 5161 6232 2118
Size: 458 943 85 1071 2901
Fragments arranged by size:
2901 1071 943 711 458 324 134 120
85 85 81 63 39
BsmI GAATG_Cn'
Cuts at: 4526 4619 4526
Size: 93 6922
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2118 2199 2238 2358 2476 2507 2640
Size: 399 328 81 39 120 118 31 133
Cuts at: 2640 2682 2970 3219 3256 3393 3468 3527 3596
Size: 42 288 249 37 137 75 59 69
Cuts at: 3596 3612 3675 3729 4050 4133 4194 4203 4334
Size: 16 63 54 321 83 61 9 131
Cuts at: 4334 5076 5161 6232 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 328 321 288 249
137 133 131 120 118 85 83 81
75 69 63 61 59 54 42 39
37 31 16 9
BspEI T'CCGG_A
Cuts at: 3393 3699 6678 3393
Size: 306 2979 3730
Fragments arranged by size:
3730 2979 306
BspHI T'CATG_A
Cuts at: 3360 4904 6634 3360
Size: 1544 1730 3741
Fragments arranged by size:
3741 1730 1544
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3444 3973 4228 4303 4370 844
Size: 2600 529 255 75 67 3489
Fragments arranged by size:
3489 2600 529 255 75 67
BsrBI CCG'CTC
Cuts at: 1394 1823 2027 2268 4902 1394
Size: 429 204 241 2634 3507
Fragments arranged by size:
3507 2634 429 241 204
BsrDI GCAATG_nn'
Cuts at: 65 1248 5510 5684 65
Size: 1183 4262 174 1396
Fragments arranged by size:
4262 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2271 2688 2850 3230 3460 4161 5659
Size: 110 461 417 162 380 230 701 1498
Cuts at: 5659 5846 1700
Size: 187 2869
Fragments arranged by size:
2869 1498 701 461 417 380 230 187
162 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7015
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7015
BssSI C'ACGA_G
Cuts at: 3781 5075 6087 3781
Size: 1294 1012 4709
Fragments arranged by size:
4709 1294 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2194 2242 794
Size: 1400 48 5567
Fragments arranged by size:
5567 1400 48
BstBI TT'CG_AA
Cuts at: 5819 5819
Size: 7015
BstEII G'GTnAC_C
Cuts at: 1184 3985 4285 1184
Size: 2801 300 3914
Fragments arranged by size:
3914 2801 300
BstXI CCAn_nnnn'nTGG
Cuts at: 3806 4282 3806
Size: 476 6539
BstYI r'GATC_y
Cuts at: 1972 2765 3411 3696 3937 4062 4345 4712 5097
Size: 793 646 285 241 125 283 367 385
Cuts at: 5097 5114 6566 6652 6664 7010 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 793 646 385 367 346 285
283 241 125 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2871 3507 3799 513
Size: 552 1806 636 292 3729
Fragments arranged by size:
3729 1806 636 552 292
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4246 487
Size: 683 56 3020 3256
Fragments arranged by size:
3256 3020 683 56
BtsI GCAGTG_nn'
Cuts at: 2543 2919 2965 3811 3848 4133 4531 5325 5345
Size: 376 46 846 37 285 398 794 20
Cuts at: 5345 2543
Size: 4213
Fragments arranged by size:
4213 846 794 398 376 285 46 37
20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7015
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6980
DraI TTT'AAA
Cuts at: 4319 4319
Size: 7015
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6022 809
Size: 507 4706 1802
Fragments arranged by size:
4706 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2269 2602 2811
Size: 54 1425 50 275 18 439 333 209
Cuts at: 2811 4462 5351 6980 8
Size: 1651 889 1629 43
Fragments arranged by size:
1651 1629 1425 889 439 333 275 209
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2269 4462 1537
Size: 275 457 2193 4090
Fragments arranged by size:
4090 2193 457 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2893 3863 4100 4945 1353
Size: 1540 970 237 845 3423
Fragments arranged by size:
3423 1540 970 845 237
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2666 3443 3509 5602 5976 6122
Size: 1064 138 1077 777 66 2093 374 146
Cuts at: 6122 387
Size: 1280
Fragments arranged by size:
2093 1280 1077 1064 777 374 146 138
66
EcoICRI GAG'CTC
Cuts at: 2680 3391 3610 4131 2680
Size: 711 219 521 5564
Fragments arranged by size:
5564 711 521 219
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2462 3332 3338 3438 3599 4136 5078
Size: 393 830 870 6 100 161 537 942
Cuts at: 5078 5675 6462 6730 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 942 870 830 787 597 537 393
268 161 100 6
EcoO109I rG'GnC_Cy
Cuts at: 3325 3592 3593 3844 3325
Size: 267 1 251 6496
Fragments arranged by size:
6496 267 251 1
EcoRI G'AATT_C
Cuts at: 4324 4702 4324
Size: 378 6637
FspI TGC'GCA
Cuts at: 3224 5521 3224
Size: 2297 4718
HaeII r_GCGC'y
Cuts at: 1678 2052 2169 2843 3237 3515 4158 4209 6162
Size: 374 117 674 394 278 643 51 1953
Cuts at: 6162 1678
Size: 2531
Fragments arranged by size:
2531 1953 674 643 394 374 278 117
51
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2121 2153 2570 2602 5656
Size: 32 135 32 549 32 417 32 3054
Cuts at: 5656 5688 5730 5762 1373
Size: 32 42 32 2626
Fragments arranged by size:
3054 2626 549 417 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2983 3370 4359 4605 669
Size: 988 281 1045 387 989 246 3079
Fragments arranged by size:
3079 1045 989 988 387 281 246
HindIII A'AGCT_T
Cuts at: 748 2360 3788 748
Size: 1612 1428 3975
Fragments arranged by size:
3975 1612 1428
HpaI GTT'AAC
Cuts at: 2983 4605 2983
Size: 1622 5393
KasI G'GCGC_C
Cuts at: 3233 3511 4205 3233
Size: 278 694 6043
Fragments arranged by size:
6043 694 278
KpnI G_GTAC'C
Cuts at: 4340 4340
Size: 7015
MfeI C'AATT_G
Cuts at: 4614 4614
Size: 7015
MluI A'CGCG_T
Cuts at: 6891 6891
Size: 7015
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6129 6313 1305
Size: 4824 184 2007
Fragments arranged by size:
4824 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2813 10
Size: 54 1425 343 981 4212
Fragments arranged by size:
4212 1425 981 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2730 2876 2945 3506 3804 4973 5332
Size: 648 1564 146 69 561 298 1169 359
Cuts at: 5332 5491 518
Size: 159 2042
Fragments arranged by size:
2042 1564 1169 648 561 359 298 159
146 69
MspA1I CmG'CkG
Cuts at: 1606 3722 3748 4225 4282 4431 5108 6256 6501
Size: 2116 26 477 57 149 677 1148 245
Cuts at: 6501 1606
Size: 2120
Fragments arranged by size:
2120 2116 1148 677 477 245 149 57
26
NaeI GCC'GGC
Cuts at: 2273 2273
Size: 7015
NarI GG'CG_CC
Cuts at: 3234 3512 4206 3234
Size: 278 694 6043
Fragments arranged by size:
6043 694 278
NcoI C'CATG_G
Cuts at: 513 1065 2871 3507 513
Size: 552 1806 636 4021
Fragments arranged by size:
4021 1806 636 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7015
NgoMIV G'CCGG_C
Cuts at: 2271 2271
Size: 7015
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7015
NotI GC'GGCC_GC
Cuts at: 4462 4462
Size: 7015
NspI r_CATG'y
Cuts at: 1132 1229 2451 2785 4373 5918 1132
Size: 97 1222 334 1588 1545 2229
Fragments arranged by size:
2229 1588 1545 1222 334 97
PasI CC'CwG_GG
Cuts at: 1348 2467 3599 1348
Size: 1119 1132 4764
Fragments arranged by size:
4764 1132 1119
PciI A'CATG_T
Cuts at: 2447 5914 2447
Size: 3467 3548
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2344 2647 1108
Size: 1236 303 5476
Fragments arranged by size:
5476 1236 303
PmeI GTTT'AAAC
Cuts at: 4319 4319
Size: 7015
PmlI CAC'GTG
Cuts at: 2521 2521
Size: 7015
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5084 5116 6597 6629 5084
Size: 32 1481 32 5470
Fragments arranged by size:
5470 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3844 3844
Size: 7015
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7015
PsiI TTA'TAA
Cuts at: 4585 4585
Size: 7015
PspOMI G'GGCC_C
Cuts at: 3252 3592 3252
Size: 340 6675
PspXI vC'TCGA_Gb
Cuts at: 2419 3131 2419
Size: 712 6303
PstI C_TGCA'G
Cuts at: 830 2048 2111 2190 2550 2838 3303 3405 3426
Size: 1218 63 79 360 288 465 102 21
Cuts at: 3426 3441 3627 4170 4367 830
Size: 15 186 543 197 3478
Fragments arranged by size:
3478 1218 543 465 360 288 197 186
102 79 63 21 15
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7015
PvuII CAG'CTG
Cuts at: 3722 4225 4282 3722
Size: 503 57 6455
Fragments arranged by size:
6455 503 57
SacI G_AGCT'C
Cuts at: 2682 3393 3612 4133 2682
Size: 711 219 521 5564
Fragments arranged by size:
5564 711 521 219
SalI G'TCGA_C
Cuts at: 1936 4357 1936
Size: 2421 4594
SanDI GG'GwC_CC
Cuts at: 3844 3844
Size: 7015
SbfI CC_TGCA'GG
Cuts at: 4367 4367
Size: 7015
ScaI AGT'ACT
Cuts at: 5263 6975 5263
Size: 1712 5303
SfcI C'TryA_G
Cuts at: 826 1045 2044 2107 2186 2546 2834 3299 3401
Size: 219 999 63 79 360 288 465 102
Cuts at: 3401 3422 3437 3623 4166 4363 5498 6179 6370
Size: 21 15 186 543 197 1135 681 191
Cuts at: 6370 826
Size: 1471
Fragments arranged by size:
1471 1135 999 681 543 465 360 288
219 197 191 186 102 79 63 21
15
SfoI GGC'GCC
Cuts at: 3235 3513 4207 3235
Size: 278 694 6043
Fragments arranged by size:
6043 694 278
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7015
SgrAI Cr'CCGG_yG
Cuts at: 1700 3230 1700
Size: 1530 5485
SmaI CCC'GGG
Cuts at: 1872 4342 1872
Size: 2470 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2419 3131 5119 6020 6282 820
Size: 197 926 476 712 1988 901 262 1553
Fragments arranged by size:
1988 1553 926 901 712 476 262 197
SnaBI TAC'GTA
Cuts at: 493 4806 493
Size: 4313 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7015
SphI G_CATG'C
Cuts at: 1132 4373 1132
Size: 3241 3774
SspI AAT'ATT
Cuts at: 5 52 4939 5
Size: 47 4887 2081
Fragments arranged by size:
4887 2081 47
StuI AGG'CCT
Cuts at: 1517 3548 1517
Size: 2031 4984
StyI C'CwwG_G
Cuts at: 513 1065 2529 2871 3507 4266 4454 513
Size: 552 1464 342 636 759 188 3074
Fragments arranged by size:
3074 1464 759 636 552 342 188
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2459 2508 2626 3520 3921 4094 5042
Size: 1255 879 49 118 894 401 173 948
Cuts at: 5042 5059 5212 5397 5842 325
Size: 17 153 185 445 1498
Fragments arranged by size:
1498 1255 948 894 879 445 401 185
173 153 118 49 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5261 6973 96
Size: 274 80 33 51 1443 3284 1712 138
Fragments arranged by size:
3284 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3139 3171 3462 3494 6975 7007 3139
Size: 32 291 32 3481 32 3147
Fragments arranged by size:
3481 3147 291 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2297 1316
Size: 981 6034
XbaI T'CTAG_A
Cuts at: 3877 4351 3877
Size: 474 6541
XhoI C'TCGA_G
Cuts at: 1943 2419 3131 1943
Size: 476 712 5827
Fragments arranged by size:
5827 712 476
XmaI C'CCGG_G
Cuts at: 1870 4340 1870
Size: 2470 4545
XmnI GAAnn'nnTTC
Cuts at: 5144 5144
Size: 7015
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4823 276
Size: 53 83 186 936 3289 2468
Fragments arranged by size:
3289 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PspXI PstI PvuI PvuII SacI SalI SanDI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BfrBI BsiWI BsmBI
BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI
NruI NsiI PacI PfoI PsrI RsrII SacII SapI
SexAI SfiI SrfI SwaI XcmI