Restriction map for
pFN21AB4623
(Circular) (Six-base) MAPSORT of: pFN21AB4623.seq Check: 6579 from: 1 to: 6265
pFN21AB4623 6265 bp
With 182 enzymes: SgfI *
October 20, 2009 10:59 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4075 278
Size: 53 83 186 936 2539 2468
Fragments arranged by size:
2539 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3608 1937
Size: 1671 4594
Acc65I G'GTAC_C
Cuts at: 3586 3586
Size: 6265
AclI AA'CG_TT
Cuts at: 1563 4392 4765 1563
Size: 2829 373 3063
Fragments arranged by size:
3063 2829 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4328 5712 5980 1632
Size: 2696 1384 268 1917
Fragments arranged by size:
2696 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6068
AflIII A'CryG_T
Cuts at: 1203 3181 5164 6141 1203
Size: 1978 1983 977 1327
Fragments arranged by size:
1983 1978 1327 977
AgeI A'CCGG_T
Cuts at: 5096 5096
Size: 6265
AhdI GACnn_n'nnGTC
Cuts at: 4994 4994
Size: 6265
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6265
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6265
AlwNI CAG_nnn'CTG
Cuts at: 4008 5580 4008
Size: 1572 4693
ApaLI G'TGCA_C
Cuts at: 4322 5478 4322
Size: 1156 5109
ApoI r'AATT_y
Cuts at: 1493 2208 2366 2689 3574 3800 3952 6061 1493
Size: 715 158 323 885 226 152 2109 1697
Fragments arranged by size:
2109 1697 885 715 323 226 158 152
AseI AT'TA_AT
Cuts at: 160 1032 4819 160
Size: 872 3787 1606
Fragments arranged by size:
3787 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6265
AvaI C'yCGr_G
Cuts at: 1870 1943 3590 1870
Size: 73 1647 4545
Fragments arranged by size:
4545 1647 73
BamHI G'GATC_C
Cuts at: 2436 3595 3962 2436
Size: 1159 367 4739
Fragments arranged by size:
4739 1159 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2171 3586 5041 618
Size: 325 217 627 384 1415 1455 1842
Fragments arranged by size:
1842 1455 1415 627 384 325 217
BanII G_rGCy'C
Cuts at: 1391 3471 3584 1391
Size: 2080 113 4072
Fragments arranged by size:
4072 2080 113
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2781 928
Size: 971 882 4412
Fragments arranged by size:
4412 971 882
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4456 4490 1903
Size: 34 2519 34 3678
Fragments arranged by size:
3678 2519 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4157 5367 4157
Size: 1210 5055
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6265
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2645 4876
Size: 107 122 71 631 605 337 636 2231
Cuts at: 4876 136
Size: 1525
Fragments arranged by size:
2231 1525 636 631 605 337 122 107
71
BglII A'GATC_T
Cuts at: 3088 6260 3088
Size: 3172 3093
BlpI GC'TnA_GC
Cuts at: 3682 3682
Size: 6265
Bme1580I G_kGCm'C
Cuts at: 1790 2423 4326 5482 1790
Size: 633 1903 1156 2573
Fragments arranged by size:
2573 1903 1156 633
BmgBI CAC'GTC
Cuts at: 1318 3184 1318
Size: 1866 4399
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3519 3996 4954
Size: 416 53 83 399 24 2102 477 958
Cuts at: 4954 442
Size: 1753
Fragments arranged by size:
2102 1753 958 477 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6265
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2949 2949
Size: 6265
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2265 2475 2754 2916 3130 4925 1239
Size: 1026 210 279 162 214 1795 2579
Fragments arranged by size:
2579 1795 1026 279 214 210 162
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2957 4390 5255 5553 2957
Size: 1433 865 298 3669
Fragments arranged by size:
3669 1433 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2344 4928 882
Size: 617 845 2584 2219
Fragments arranged by size:
2584 2219 845 617
BsaAI yAC'GTr
Cuts at: 493 1204 4056 493
Size: 711 2852 2702
Fragments arranged by size:
2852 2702 711
BsaBI GATnn'nnATC
Cuts at: 3087 6259 3087
Size: 3172 3093
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4072 4454 5089 275
Size: 53 83 186 936 2539 382 635 1451
Fragments arranged by size:
2539 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2856 4698 5096 5370 5517 5928 2856
Size: 1842 398 274 147 411 3193
Fragments arranged by size:
3193 1842 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2889 2919 1178
Size: 30 165 30 1486 30 4524
Fragments arranged by size:
4524 1486 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2012 2017 1186
Size: 826 5 5434
Fragments arranged by size:
5434 826 5
BseYI C'CCAG_C
Cuts at: 5468 5964 5468
Size: 496 5769
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2463 1163
Size: 573 727 4965
Fragments arranged by size:
4965 727 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3715 4476 5504 1540
Size: 275 186 1714 761 1028 2301
Fragments arranged by size:
2301 1714 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2113 3471 4326 4411 5482 2113
Size: 1358 855 85 1071 2896
Fragments arranged by size:
2896 1358 1071 855 85
BsmI GAATG_Cn'
Cuts at: 3331 3776 3869 3331
Size: 445 93 5727
Fragments arranged by size:
5727 445 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2113 2423 3471 3584 4326 4411 5482
Size: 399 323 310 1048 113 742 85 1071
Cuts at: 5482 1391
Size: 2174
Fragments arranged by size:
2174 1071 1048 742 399 323 310 113
85
BspEI T'CCGG_A
Cuts at: 2856 5928 2856
Size: 3072 3193
BspHI T'CATG_A
Cuts at: 4154 5884 4154
Size: 1730 4535
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3202 3620 844
Size: 2358 418 3489
Fragments arranged by size:
3489 2358 418
BsrBI CCG'CTC
Cuts at: 1394 1823 2933 4152 1394
Size: 429 1110 1219 3507
Fragments arranged by size:
3507 1219 1110 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2547 3171 3401 4760 4934 65
Size: 1183 1299 624 230 1359 174 1396
Fragments arranged by size:
1396 1359 1299 1183 624 230 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4909 5096 1700
Size: 110 3099 187 2869
Fragments arranged by size:
3099 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2800 3371 96
Size: 2704 571 2990
Fragments arranged by size:
2990 2704 571
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6265
BssSI C'ACGA_G
Cuts at: 4325 5337 4325
Size: 1012 5253
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6265
BstBI TT'CG_AA
Cuts at: 5069 5069
Size: 6265
BstEII G'GTnAC_C
Cuts at: 1184 3227 3319 1184
Size: 2043 92 4130
Fragments arranged by size:
4130 2043 92
BstXI CCAn_nnnn'nTGG
Cuts at: 2182 3316 2182
Size: 1134 5131
BstYI r'GATC_y
Cuts at: 1972 2060 2137 2436 3088 3595 3962 4347 4364
Size: 88 77 299 652 507 367 385 17
Cuts at: 4364 5816 5902 5914 6260 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 652 507 385 367 346 299
88 86 77 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2056 2175 3324 513
Size: 552 939 52 119 1149 3454
Fragments arranged by size:
3454 1149 939 552 119 52
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2099 2789 3165 3221 487
Size: 683 56 873 690 376 56 3531
Fragments arranged by size:
3531 873 690 683 376 56 56
BtsI GCAGTG_nn'
Cuts at: 2545 3781 4575 4595 2545
Size: 1236 794 20 4215
Fragments arranged by size:
4215 1236 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6265
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6230
DraI TTT'AAA
Cuts at: 3569 3569
Size: 6265
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5272 809
Size: 507 3956 1802
Fragments arranged by size:
3956 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2988 3712 4601
Size: 54 1425 50 275 18 1158 724 889
Cuts at: 4601 6230 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1158 889 724 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3712 1537
Size: 275 1900 4090
Fragments arranged by size:
4090 1900 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2347 3497 4195 1353
Size: 994 1150 698 3423
Fragments arranged by size:
3423 1150 994 698
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2663 4852 5226 5372 387
Size: 1064 138 1074 2189 374 146 1280
Fragments arranged by size:
2189 1280 1074 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3469 3469
Size: 6265
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2265 2475 2754 2916 3130 4328 4925
Size: 393 633 210 279 162 214 1198 597
Cuts at: 4925 5712 5980 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1198 787 633 597 393 279 268
214 210 162
EcoNI CCTnn'n_nnAGG
Cuts at: 2813 2813
Size: 6265
EcoO109I rG'GnC_Cy
Cuts at: 2490 2490
Size: 6265
EcoRI G'AATT_C
Cuts at: 2208 3574 3952 2208
Size: 1366 378 4521
Fragments arranged by size:
4521 1366 378
FspI TGC'GCA
Cuts at: 4771 4771
Size: 6265
HaeII r_GCGC'y
Cuts at: 1678 5412 1678
Size: 3734 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2502 2534 2834 2866 3278
Size: 32 135 32 930 32 300 32 412
Cuts at: 3278 3310 4906 4938 4980 5012 1373
Size: 32 1596 32 42 32 2626
Fragments arranged by size:
2626 1596 930 412 300 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2287 3403 3456 3609 3855 669
Size: 988 281 349 1116 53 153 246 3079
Fragments arranged by size:
3079 1116 988 349 281 246 153 53
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6265
HpaI GTT'AAC
Cuts at: 3855 3855
Size: 6265
KpnI G_GTAC'C
Cuts at: 3590 3590
Size: 6265
MfeI C'AATT_G
Cuts at: 3864 3864
Size: 6265
MluI A'CGCG_T
Cuts at: 6141 6141
Size: 6265
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5379 5563 1305
Size: 4074 184 2007
Fragments arranged by size:
4074 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2990 10
Size: 54 1425 343 1158 3285
Fragments arranged by size:
3285 1425 1158 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3545 4223 4582 4741 518
Size: 648 2379 678 359 159 2042
Fragments arranged by size:
2379 2042 678 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2058 3681 4358 5506 5751 1606
Size: 452 1623 677 1148 245 2120
Fragments arranged by size:
2120 1623 1148 677 452 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 3324 513
Size: 552 939 1320 3454
Fragments arranged by size:
3454 1320 939 552
NdeI CA'TA_TG
Cuts at: 387 2347 387
Size: 1960 4305
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6265
NotI GC'GGCC_GC
Cuts at: 3712 3712
Size: 6265
NspI r_CATG'y
Cuts at: 1132 1229 2956 3623 5168 1132
Size: 97 1727 667 1545 2229
Fragments arranged by size:
2229 1727 1545 667 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6265
PciI A'CATG_T
Cuts at: 5164 5164
Size: 6265
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3386 1108
Size: 2278 3987
PfoI T'CCnGG_A
Cuts at: 3127 3127
Size: 6265
PmeI GTTT'AAAC
Cuts at: 3569 3569
Size: 6265
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4334 4366 5847 5879 4334
Size: 32 1481 32 4720
Fragments arranged by size:
4720 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2490 2490
Size: 6265
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6265
PsiI TTA'TAA
Cuts at: 2813 3835 2813
Size: 1022 5243
PstI C_TGCA'G
Cuts at: 830 2266 2719 3617 830
Size: 1436 453 898 3478
Fragments arranged by size:
3478 1436 898 453
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6265
RsrII CG'GwC_CG
Cuts at: 2650 2650
Size: 6265
SacI G_AGCT'C
Cuts at: 3471 3471
Size: 6265
SacII CC_GC'GG
Cuts at: 2059 2059
Size: 6265
SalI G'TCGA_C
Cuts at: 1936 3607 1936
Size: 1671 4594
SanDI GG'GwC_CC
Cuts at: 2490 2490
Size: 6265
SbfI CC_TGCA'GG
Cuts at: 3617 3617
Size: 6265
ScaI AGT'ACT
Cuts at: 2381 3508 4513 6225 2381
Size: 1127 1005 1712 2421
Fragments arranged by size:
2421 1712 1127 1005
SexAI A'CCwGG_T
Cuts at: 2072 2072
Size: 6265
SfcI C'TryA_G
Cuts at: 826 1045 2262 2715 3613 4748 5429 5620 826
Size: 219 1217 453 898 1135 681 191 1471
Fragments arranged by size:
1471 1217 1135 898 681 453 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6265
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6265
SmaI CCC'GGG
Cuts at: 1872 3592 1872
Size: 1720 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2936 4369 5270 5532 820
Size: 197 926 993 1433 901 262 1553
Fragments arranged by size:
1553 1433 993 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4056 493
Size: 3563 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6265
SphI G_CATG'C
Cuts at: 1132 3623 1132
Size: 2491 3774
SspI AAT'ATT
Cuts at: 5 52 4189 5
Size: 47 4137 2081
Fragments arranged by size:
4137 2081 47
StuI AGG'CCT
Cuts at: 1517 3480 1517
Size: 1963 4302
StyI C'CwwG_G
Cuts at: 513 1065 2004 3324 3704 513
Size: 552 939 1320 380 3074
Fragments arranged by size:
3074 1320 939 552 380
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2304 2667 3501 4292 4309 4462 4647
Size: 1255 724 363 834 791 17 153 185
Cuts at: 4647 5092 325
Size: 445 1498
Fragments arranged by size:
1498 1255 834 791 724 445 363 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2379 2800 3371
Size: 274 80 33 51 1443 402 421 571
Cuts at: 3371 3506 4511 6223 96
Size: 135 1005 1712 138
Fragments arranged by size:
1712 1443 1005 571 421 402 274 138
135 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6225 6257 6225
Size: 32 6233
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6265
XbaI T'CTAG_A
Cuts at: 3601 3601
Size: 6265
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6265
XmaI C'CCGG_G
Cuts at: 1870 3590 1870
Size: 1720 4545
XmnI GAAnn'nnTTC
Cuts at: 4394 4394
Size: 6265
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4073 276
Size: 53 83 186 936 2539 2468
Fragments arranged by size:
2539 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI RsrII SacI SacII SalI SanDI SbfI
ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PspOMI PspXI PsrI PvuII
SapI SfiI SfoI SrfI SwaI XcmI