Restriction map for
pFN21AB4618
(Circular) (Six-base) MAPSORT of: pFN21AB4618.seq Check: 7843 from: 1 to: 6943
pFN21AB4618 6943 bp
With 182 enzymes: SgfI *
February 27, 2009 14:35 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4753 278
Size: 53 83 186 936 3217 2468
Fragments arranged by size:
3217 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3911 4286 1937
Size: 1974 375 4594
Fragments arranged by size:
4594 1974 375
Acc65I G'GTAC_C
Cuts at: 4264 4264
Size: 6943
AclI AA'CG_TT
Cuts at: 1563 5070 5443 1563
Size: 3507 373 3063
Fragments arranged by size:
3507 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3168 3531 4116 4235 5006 6390 6658 1632
Size: 1536 363 585 119 771 1384 268 1917
Fragments arranged by size:
1917 1536 1384 771 585 363 268 119
AfeI AGC'GCT
Cuts at: 2984 2984
Size: 6943
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6746
AflIII A'CryG_T
Cuts at: 1203 2352 2354 2658 5842 6819 1203
Size: 1149 2 304 3184 977 1327
Fragments arranged by size:
3184 1327 1149 977 304 2
AgeI A'CCGG_T
Cuts at: 3771 5774 3771
Size: 2003 4940
AhdI GACnn_n'nnGTC
Cuts at: 5672 5672
Size: 6943
AleI CACnn'nnGTG
Cuts at: 1166 3512 1166
Size: 2346 4597
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6943
AlwNI CAG_nnn'CTG
Cuts at: 3686 4686 6258 3686
Size: 1000 1572 4371
Fragments arranged by size:
4371 1572 1000
ApaI G_GGCC'C
Cuts at: 2719 2719
Size: 6943
ApaLI G'TGCA_C
Cuts at: 4057 5000 6156 4057
Size: 943 1156 4844
Fragments arranged by size:
4844 1156 943
ApoI r'AATT_y
Cuts at: 1493 3761 4040 4252 4478 4630 6739 1493
Size: 2268 279 212 226 152 2109 1697
Fragments arranged by size:
2268 2109 1697 279 226 212 152
AseI AT'TA_AT
Cuts at: 160 1032 5497 160
Size: 872 4465 1606
Fragments arranged by size:
4465 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6943
AvaI C'yCGr_G
Cuts at: 1870 1943 2469 3787 4268 1870
Size: 73 526 1318 481 4545
Fragments arranged by size:
4545 1318 526 481 73
BamHI G'GATC_C
Cuts at: 4273 4640 4273
Size: 367 6576
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2011 3523 4264 5719 618
Size: 325 217 627 224 1512 741 1455 1842
Fragments arranged by size:
1842 1512 1455 741 627 325 224 217
BanII G_rGCy'C
Cuts at: 1391 2719 3061 4262 1391
Size: 1328 342 1201 4072
Fragments arranged by size:
4072 1328 1201 342
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2150 2995 4129 928
Size: 971 251 845 1134 3742
Fragments arranged by size:
3742 1134 971 845 251
BbvCI CC'TCA_GC
Cuts at: 2802 2802
Size: 6943
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5134 5168 1903
Size: 34 3197 34 3678
Fragments arranged by size:
3678 3197 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4835 6045 4835
Size: 1210 5733
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6943
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2723 5554 136
Size: 107 122 71 631 605 1051 2831 1525
Fragments arranged by size:
2831 1525 1051 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6938 6938
Size: 6943
BlpI GC'TnA_GC
Cuts at: 4360 4360
Size: 6943
Bme1580I G_kGCm'C
Cuts at: 1790 2719 3528 4061 5004 6160 1790
Size: 929 809 533 943 1156 2573
Fragments arranged by size:
2573 1156 943 929 809 533
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6943
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3522 3882 3952
Size: 416 53 83 399 24 2105 360 70
Cuts at: 3952 4674 5632 442
Size: 722 958 1753
Fragments arranged by size:
2105 1753 958 722 416 399 360 83
70 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6943
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2688 2888 3561 5603 1239
Size: 1449 200 673 2042 2579
Fragments arranged by size:
2579 2042 1449 673 200
Bpu10I CC'TnA_GC
Cuts at: 2802 2802
Size: 6943
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3432 4139 5068 5933 6231 3432
Size: 707 929 865 298 4144
Fragments arranged by size:
4144 929 865 707 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2873 5606 882
Size: 617 1374 2733 2219
Fragments arranged by size:
2733 2219 1374 617
BsaAI yAC'GTr
Cuts at: 493 1204 2355 2431 2745 4734 493
Size: 711 1151 76 314 1989 2702
Fragments arranged by size:
2702 1989 1151 711 314 76
BsaBI GATnn'nnATC
Cuts at: 2367 6937 2367
Size: 4570 2373
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2445 4750 5132 5767
Size: 53 83 186 936 912 2305 382 635
Cuts at: 5767 275
Size: 1451
Fragments arranged by size:
2305 1451 936 912 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3336 3771 5376 5774 6048 6195 6606 3336
Size: 435 1605 398 274 147 411 3673
Fragments arranged by size:
3673 1605 435 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2450 2480 2910 2940 3408
Size: 30 165 30 1047 30 430 30 468
Cuts at: 3408 3438 1178
Size: 30 4683
Fragments arranged by size:
4683 1047 468 430 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6943
BseYI C'CCAG_C
Cuts at: 2613 3064 6146 6642 2613
Size: 451 3082 496 2914
Fragments arranged by size:
3082 2914 496 451
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6370
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3481 4393 5154 6182 1540
Size: 275 186 1480 912 761 1028 2301
Fragments arranged by size:
2301 1480 1028 912 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3061 3870 4061 5004 5089 6160 3061
Size: 809 191 943 85 1071 3844
Fragments arranged by size:
3844 1071 943 809 191 85
BsmI GAATG_Cn'
Cuts at: 2352 2775 4454 4547 2352
Size: 423 1679 93 4748
Fragments arranged by size:
4748 1679 423 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2452 2452
Size: 6943
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2719 3061 3528 3870 4061 4262 5004
Size: 399 929 342 467 342 191 201 742
Cuts at: 5004 5089 6160 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 929 742 467 399 342 342
201 191 85
BspEI T'CCGG_A
Cuts at: 6606 6606
Size: 6943
BspHI T'CATG_A
Cuts at: 4832 6562 4832
Size: 1730 5213
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4298 844
Size: 3454 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2326 4830 1394
Size: 429 503 2504 3507
Fragments arranged by size:
3507 2504 503 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2199 3117 3303 5438 5612 65
Size: 1183 951 918 186 2135 174 1396
Fragments arranged by size:
2135 1396 1183 951 918 186 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2287 2754 3771 5587 5774 1700
Size: 110 477 467 1017 1816 187 2869
Fragments arranged by size:
2869 1816 1017 477 467 187 110
BsrGI T'GTAC_A
Cuts at: 96 3207 4188 96
Size: 3111 981 2851
Fragments arranged by size:
3111 2851 981
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6943
BssSI C'ACGA_G
Cuts at: 5003 6015 5003
Size: 1012 5931
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6943
BstBI TT'CG_AA
Cuts at: 5747 5747
Size: 6943
BstEII G'GTnAC_C
Cuts at: 1184 3782 4024 1184
Size: 2598 242 4103
Fragments arranged by size:
4103 2598 242
BstXI CCAn_nnnn'nTGG
Cuts at: 3310 3310
Size: 6943
BstYI r'GATC_y
Cuts at: 1972 2332 2809 3339 4273 4640 5025 5042 6494
Size: 360 477 530 934 367 385 17 1452
Cuts at: 6494 6580 6592 6938 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 934 530 477 385 367 360
346 86 17 12
BstZ17I GTA'TAC
Cuts at: 3912 3912
Size: 6943
BtgI C'CryG_G
Cuts at: 513 1065 2004 2036 2834 3861 513
Size: 552 939 32 798 1027 3595
Fragments arranged by size:
3595 1027 939 798 552 32
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2621 3099 487
Size: 683 56 1395 478 4331
Fragments arranged by size:
4331 1395 683 478 56
BtsI GCAGTG_nn'
Cuts at: 2579 4459 5253 5273 2579
Size: 1880 794 20 4249
Fragments arranged by size:
4249 1880 794 20
ClaI AT'CG_AT
Cuts at: 1554 2559 1554
Size: 1005 5938
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6908
DraI TTT'AAA
Cuts at: 4247 4247
Size: 6943
DraIII CAC_nnn'GTG
Cuts at: 4099 4099
Size: 6943
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2444 5950 809
Size: 507 1128 3506 1802
Fragments arranged by size:
3506 1802 1128 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2033 2493 3312
Size: 54 1425 50 275 18 203 460 819
Cuts at: 3312 3693 3993 4390 5279 6908 8
Size: 381 300 397 889 1629 43
Fragments arranged by size:
1629 1425 889 819 460 397 381 300
275 203 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4390 1537
Size: 275 2578 4090
Fragments arranged by size:
4090 2578 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2026 2064 2180 3158 3425 3500 4211 4873
Size: 673 38 116 978 267 75 711 662
Cuts at: 4873 1353
Size: 3423
Fragments arranged by size:
3423 978 711 673 662 267 116 75
38
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5530 5904 6050 387
Size: 1064 138 3941 374 146 1280
Fragments arranged by size:
3941 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3059 3059
Size: 6943
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2688 2888 3168 3531 3561 4116 4235
Size: 393 1056 200 280 363 30 555 119
Cuts at: 4235 5006 5603 6390 6658 1239
Size: 771 597 787 268 1524
Fragments arranged by size:
1524 1056 787 771 597 555 393 363
280 268 200 119 30
EcoNI CCTnn'n_nnAGG
Cuts at: 2957 2957
Size: 6943
EcoO109I rG'GnC_Cy
Cuts at: 2120 2672 2715 2716 2890 2120
Size: 552 43 1 174 6173
Fragments arranged by size:
6173 552 174 43 1
EcoRI G'AATT_C
Cuts at: 4252 4630 4252
Size: 378 6565
EcoRV GAT'ATC
Cuts at: 3709 3934 3709
Size: 225 6718
FspI TGC'GCA
Cuts at: 2585 5449 2585
Size: 2864 4079
HaeII r_GCGC'y
Cuts at: 1678 2986 6090 1678
Size: 1308 3104 2531
Fragments arranged by size:
3104 2531 1308
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4135 4167 5584 5616 5658
Size: 32 135 32 2563 32 1417 32 42
Cuts at: 5658 5690 1373
Size: 32 2626
Fragments arranged by size:
2626 2563 1417 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4287 4533 669
Size: 988 281 2349 246 3079
Fragments arranged by size:
3079 2349 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6943
HpaI GTT'AAC
Cuts at: 4533 4533
Size: 6943
KpnI G_GTAC'C
Cuts at: 4268 4268
Size: 6943
MfeI C'AATT_G
Cuts at: 4542 4542
Size: 6943
MluI A'CGCG_T
Cuts at: 6819 6819
Size: 6943
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6057 6241 1305
Size: 4752 184 2007
Fragments arranged by size:
4752 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2035 2495 3314 3695 3995
Size: 54 1425 343 203 460 819 381 300
Cuts at: 3995 10
Size: 2958
Fragments arranged by size:
2958 1425 819 460 381 343 300 203
54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2210 2561 3512 4901 5260 5419
Size: 648 843 201 351 951 1389 359 159
Cuts at: 5419 518
Size: 2042
Fragments arranged by size:
2042 1389 951 843 648 359 351 201
159
MspA1I CmG'CkG
Cuts at: 1606 2801 3430 4359 5036 6184 6429 1606
Size: 1195 629 929 677 1148 245 2120
Fragments arranged by size:
2120 1195 1148 929 677 629 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 3861 513
Size: 552 939 1857 3595
Fragments arranged by size:
3595 1857 939 552
NdeI CA'TA_TG
Cuts at: 387 3349 3957 387
Size: 2962 608 3373
Fragments arranged by size:
3373 2962 608
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6943
NotI GC'GGCC_GC
Cuts at: 4390 4390
Size: 6943
NspI r_CATG'y
Cuts at: 1132 1229 2215 2662 4301 5846 1132
Size: 97 986 447 1639 1545 2229
Fragments arranged by size:
2229 1639 1545 986 447 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6943
PciI A'CATG_T
Cuts at: 2658 5842 2658
Size: 3184 3759
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6943
PmeI GTTT'AAAC
Cuts at: 4247 4247
Size: 6943
PmlI CAC'GTG
Cuts at: 2355 2355
Size: 6943
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5012 5044 6525 6557 5012
Size: 32 1481 32 5398
Fragments arranged by size:
5398 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2120 2672 2890 2120
Size: 552 218 6173
Fragments arranged by size:
6173 552 218
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6943
PsiI TTA'TAA
Cuts at: 2685 4513 2685
Size: 1828 5115
PspOMI G'GGCC_C
Cuts at: 2715 2715
Size: 6943
PstI C_TGCA'G
Cuts at: 830 3324 4295 830
Size: 2494 971 3478
Fragments arranged by size:
3478 2494 971
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6943
PvuII CAG'CTG
Cuts at: 2801 2801
Size: 6943
SacI G_AGCT'C
Cuts at: 3061 3061
Size: 6943
SalI G'TCGA_C
Cuts at: 1936 4285 1936
Size: 2349 4594
SapI GCTCTTCn'nnn_
Cuts at: 2026 4211 2026
Size: 2185 4758
SbfI CC_TGCA'GG
Cuts at: 4295 4295
Size: 6943
ScaI AGT'ACT
Cuts at: 2934 5191 6903 2934
Size: 2257 1712 2974
Fragments arranged by size:
2974 2257 1712
SexAI A'CCwGG_T
Cuts at: 2827 2997 2827
Size: 170 6773
SfcI C'TryA_G
Cuts at: 826 1045 2785 3023 3320 4291 5426 6107 6298
Size: 219 1740 238 297 971 1135 681 191
Cuts at: 6298 826
Size: 1471
Fragments arranged by size:
1740 1471 1135 971 681 297 238 219
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6943
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6943
SmaI CCC'GGG
Cuts at: 1872 2471 3789 4270 1872
Size: 599 1318 481 4545
Fragments arranged by size:
4545 1318 599 481
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3447 4154 5047 5948 6210 820
Size: 197 926 1504 707 893 901 262 1553
Fragments arranged by size:
1553 1504 926 901 893 707 262 197
SnaBI TAC'GTA
Cuts at: 493 4734 493
Size: 4241 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6943
SphI G_CATG'C
Cuts at: 1132 4301 1132
Size: 3169 3774
SspI AAT'ATT
Cuts at: 5 52 4867 5
Size: 47 4815 2081
Fragments arranged by size:
4815 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6943
StyI C'CwwG_G
Cuts at: 513 1065 2004 3519 3861 4382 513
Size: 552 939 1515 342 521 3074
Fragments arranged by size:
3074 1515 939 552 521 342
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2089 3495 4970 4987 5140 5325 5770
Size: 1255 509 1406 1475 17 153 185 445
Cuts at: 5770 325
Size: 1498
Fragments arranged by size:
1498 1475 1406 1255 509 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2880 2932 3207
Size: 274 80 33 51 1443 903 52 275
Cuts at: 3207 4188 5189 6901 96
Size: 981 1001 1712 138
Fragments arranged by size:
1712 1443 1001 981 903 275 274 138
80 52 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2910 2942 3408 3440 6903 6935 2910
Size: 32 466 32 3463 32 2918
Fragments arranged by size:
3463 2918 466 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2829 4181 1316
Size: 1513 1352 4078
Fragments arranged by size:
4078 1513 1352
XbaI T'CTAG_A
Cuts at: 4279 4279
Size: 6943
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2188 2912 3985 2188
Size: 724 1073 5146
Fragments arranged by size:
5146 1073 724
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6943
XmaI C'CCGG_G
Cuts at: 1870 2469 3787 4268 1870
Size: 599 1318 481 4545
Fragments arranged by size:
4545 1318 599 481
XmnI GAAnn'nnTTC
Cuts at: 3687 4220 5072 3687
Size: 533 852 5558
Fragments arranged by size:
5558 852 533
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4751 276
Size: 53 83 186 936 3217 2468
Fragments arranged by size:
3217 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SacI SalI SapI SbfI ScaI
SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbeI BfrBI BplI
BsiWI Bsu36I FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PspXI PsrI RsrII
SacII SanDI SfiI SfoI SrfI SwaI