Restriction map for
pFN21AB4613
(Circular) (Six-base) MAPSORT of: pFN21AB4613.seq Check: 7146 from: 1 to: 5500
pFN21AB4613 5500 bp
With 182 enzymes: SgfI *
January 16, 2009 15:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3310 278
Size: 53 83 186 936 1774 2468
Fragments arranged by size:
2468 1774 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2843 1937
Size: 906 4594
Acc65I G'GTAC_C
Cuts at: 2821 2821
Size: 5500
AclI AA'CG_TT
Cuts at: 1563 3627 4000 1563
Size: 2064 373 3063
Fragments arranged by size:
3063 2064 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2462 3563 4947 5215 1632
Size: 830 1101 1384 268 1917
Fragments arranged by size:
1917 1384 1101 830 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5303
AflIII A'CryG_T
Cuts at: 1203 2373 2430 4399 5376 1203
Size: 1170 57 1969 977 1327
Fragments arranged by size:
1969 1327 1170 977 57
AgeI A'CCGG_T
Cuts at: 4331 4331
Size: 5500
AhdI GACnn_n'nnGTC
Cuts at: 4229 4229
Size: 5500
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5500
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5500
AlwNI CAG_nnn'CTG
Cuts at: 2122 3243 4815 2122
Size: 1121 1572 2807
Fragments arranged by size:
2807 1572 1121
ApaLI G'TGCA_C
Cuts at: 2060 3557 4713 2060
Size: 1497 1156 2847
Fragments arranged by size:
2847 1497 1156
ApoI r'AATT_y
Cuts at: 1493 2809 3035 3187 5296 1493
Size: 1316 226 152 2109 1697
Fragments arranged by size:
2109 1697 1316 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4054 160
Size: 872 3022 1606
Fragments arranged by size:
3022 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5500
AvaI C'yCGr_G
Cuts at: 1870 1943 2485 2825 1870
Size: 73 542 340 4545
Fragments arranged by size:
4545 542 340 73
BamHI G'GATC_C
Cuts at: 2830 3197 2830
Size: 367 5133
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2357 2732 2821 4276 618
Size: 325 217 627 570 375 89 1455 1842
Fragments arranged by size:
1842 1455 627 570 375 325 217 89
BanII G_rGCy'C
Cuts at: 1391 2575 2819 1391
Size: 1184 244 4072
Fragments arranged by size:
4072 1184 244
BbeI G_GCGC'C
Cuts at: 2736 2736
Size: 5500
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4529
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3691 3725 1903
Size: 34 1754 34 3678
Fragments arranged by size:
3678 1754 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3392 4602 3392
Size: 1210 4290
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5500
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2226 4111 136
Size: 107 122 71 631 605 554 1885 1525
Fragments arranged by size:
1885 1525 631 605 554 122 107 71
BglII A'GATC_T
Cuts at: 5495 5495
Size: 5500
BlpI GC'TnA_GC
Cuts at: 2168 2417 2917 2168
Size: 249 500 4751
Fragments arranged by size:
4751 500 249
Bme1580I G_kGCm'C
Cuts at: 1790 2064 2360 3561 4717 1790
Size: 274 296 1201 1156 2573
Fragments arranged by size:
2573 1201 1156 296 274
BmgBI CAC'GTC
Cuts at: 1318 2341 1318
Size: 1023 4477
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2330 3231 4189
Size: 416 53 83 399 24 913 901 958
Cuts at: 4189 442
Size: 1753
Fragments arranged by size:
1753 958 913 901 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5500
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2494 2641 2494
Size: 147 5353
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2029 2300 2597 2669 4160 1239
Size: 790 271 297 72 1491 2579
Fragments arranged by size:
2579 1491 790 297 271 72
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3625 4490 4788 3625
Size: 865 298 4337
Fragments arranged by size:
4337 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4163 882
Size: 617 2664 2219
Fragments arranged by size:
2664 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3291 493
Size: 711 2087 2702
Fragments arranged by size:
2702 2087 711
BsaBI GATnn'nnATC
Cuts at: 5494 5494
Size: 5500
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2733 2789 3307 3689
Size: 53 83 186 936 1200 56 518 382
Cuts at: 3689 4324 275
Size: 635 1451
Fragments arranged by size:
1451 1200 936 635 518 382 186 83
56 53
BsaWI w'CCGG_w
Cuts at: 3933 4331 4605 4752 5163 3933
Size: 398 274 147 411 4270
Fragments arranged by size:
4270 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5275
Fragments arranged by size:
5275 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2483 1186
Size: 1297 4203
BseYI C'CCAG_C
Cuts at: 4703 5199 4703
Size: 496 5004
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2147 2804 1163
Size: 573 411 657 3859
Fragments arranged by size:
3859 657 573 411
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2013 2950 3711 4739 1540
Size: 275 186 12 937 761 1028 2301
Fragments arranged by size:
2301 1028 937 761 275 186 12
BsiHKAI G_wGCw'C
Cuts at: 2064 3561 3646 4717 2064
Size: 1497 85 1071 2847
Fragments arranged by size:
2847 1497 1071 85
BsmI GAATG_Cn'
Cuts at: 3011 3104 3011
Size: 93 5407
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2064 2360 2575 2819 3561 3646 4717
Size: 399 274 296 215 244 742 85 1071
Cuts at: 4717 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 296 274 244 215
85
BspEI T'CCGG_A
Cuts at: 5163 5163
Size: 5500
BspHI T'CATG_A
Cuts at: 3389 5119 3389
Size: 1730 3770
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2388 2855 844
Size: 1544 467 3489
Fragments arranged by size:
3489 1544 467
BsrBI CCG'CTC
Cuts at: 1394 1823 2167 3387 1394
Size: 429 344 1220 3507
Fragments arranged by size:
3507 1220 429 344
BsrDI GCAATG_nn'
Cuts at: 65 1248 3995 4169 65
Size: 1183 2747 174 1396
Fragments arranged by size:
2747 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4144 4331 1700
Size: 110 2334 187 2869
Fragments arranged by size:
2869 2334 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5500
BssHII G'CGCG_C
Cuts at: 1925 2229 1925
Size: 304 5196
BssSI C'ACGA_G
Cuts at: 2497 3560 4572 2497
Size: 1063 1012 3425
Fragments arranged by size:
3425 1063 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5500
BstBI TT'CG_AA
Cuts at: 4304 4304
Size: 5500
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5500
BstYI r'GATC_y
Cuts at: 1972 2830 3197 3582 3599 5051 5137 5149 5495
Size: 858 367 385 17 1452 86 12 346
Cuts at: 5495 1972
Size: 1977
Fragments arranged by size:
1977 1452 858 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2776 513
Size: 552 939 772 3237
Fragments arranged by size:
3237 939 772 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4761
Fragments arranged by size:
4761 683 56
BtsI GCAGTG_nn'
Cuts at: 3016 3810 3830 3016
Size: 794 20 4686
Fragments arranged by size:
4686 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5500
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5465
DraI TTT'AAA
Cuts at: 2804 2804
Size: 5500
DraIII CAC_nnn'GTG
Cuts at: 2782 2782
Size: 5500
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2017 4507 809
Size: 507 701 2490 1802
Fragments arranged by size:
2490 1802 701 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2218 2947 3836
Size: 54 1425 50 275 18 388 729 889
Cuts at: 3836 5465 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 729 388 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2947 1537
Size: 275 1135 4090
Fragments arranged by size:
4090 1135 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3430 1353
Size: 2077 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2202 2326 2489 4087 4461 4607
Size: 1064 138 613 124 163 1598 374 146
Cuts at: 4607 387
Size: 1280
Fragments arranged by size:
1598 1280 1064 613 374 163 146 138
124
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2029 2300 2462 2597 2669 3563 4160
Size: 393 397 271 162 135 72 894 597
Cuts at: 4160 4947 5215 1239
Size: 787 268 1524
Fragments arranged by size:
1524 894 787 597 397 393 271 268
162 135 72
EcoNI CCTnn'n_nnAGG
Cuts at: 2126 2715 2126
Size: 589 4911
EcoO109I rG'GnC_Cy
Cuts at: 2407 2407
Size: 5500
EcoRI G'AATT_C
Cuts at: 2809 3187 2809
Size: 378 5122
FspI TGC'GCA
Cuts at: 4006 4006
Size: 5500
HaeII r_GCGC'y
Cuts at: 1678 2052 2145 2736 4647 1678
Size: 374 93 591 1911 2531
Fragments arranged by size:
2531 1911 591 374 93
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2151 2183 2231 2263 2468
Size: 32 135 32 579 32 48 32 205
Cuts at: 2468 2500 2695 2727 4141 4173 4215 4247 1373
Size: 32 195 32 1414 32 42 32 2626
Fragments arranged by size:
2626 1414 579 205 195 135 48 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2844 3090 669
Size: 988 281 906 246 3079
Fragments arranged by size:
3079 988 906 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5500
HpaI GTT'AAC
Cuts at: 3090 3090
Size: 5500
KasI G'GCGC_C
Cuts at: 2732 2732
Size: 5500
KpnI G_GTAC'C
Cuts at: 2825 2825
Size: 5500
MfeI C'AATT_G
Cuts at: 3099 3099
Size: 5500
MluI A'CGCG_T
Cuts at: 5376 5376
Size: 5500
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2445 4614 4798 1305
Size: 1140 2169 184 2007
Fragments arranged by size:
2169 2007 1140 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2220 10
Size: 54 1425 343 388 3290
Fragments arranged by size:
3290 1425 388 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2555 3458 3817 3976 518
Size: 648 1389 903 359 159 2042
Fragments arranged by size:
2042 1389 903 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2308 2416 2647 2916 3593 4741 4986 1606
Size: 702 108 231 269 677 1148 245 2120
Fragments arranged by size:
2120 1148 702 677 269 245 231 108
NarI GG'CG_CC
Cuts at: 2733 2733
Size: 5500
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4009
Fragments arranged by size:
4009 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5500
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5500
NotI GC'GGCC_GC
Cuts at: 2947 2947
Size: 5500
NspI r_CATG'y
Cuts at: 1132 1229 2086 2434 2858 4403 1132
Size: 97 857 348 424 1545 2229
Fragments arranged by size:
2229 1545 857 424 348 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5500
PciI A'CATG_T
Cuts at: 2430 4399 2430
Size: 1969 3531
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5500
PfoI T'CCnGG_A
Cuts at: 2713 2713
Size: 5500
PmeI GTTT'AAAC
Cuts at: 2804 2804
Size: 5500
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3569 3601 5082 5114 3569
Size: 32 1481 32 3955
Fragments arranged by size:
3955 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2792 1613
Size: 1179 4321
PsiI TTA'TAA
Cuts at: 3070 3070
Size: 5500
PstI C_TGCA'G
Cuts at: 830 2151 2399 2415 2852 830
Size: 1321 248 16 437 3478
Fragments arranged by size:
3478 1321 437 248 16
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5500
PvuII CAG'CTG
Cuts at: 2416 2647 2416
Size: 231 5269
SalI G'TCGA_C
Cuts at: 1936 2842 1936
Size: 906 4594
SbfI CC_TGCA'GG
Cuts at: 2852 2852
Size: 5500
ScaI AGT'ACT
Cuts at: 3748 5460 3748
Size: 1712 3788
SfcI C'TryA_G
Cuts at: 826 1045 2029 2147 2395 2411 2848 3983 4664
Size: 219 984 118 248 16 437 1135 681
Cuts at: 4664 4855 826
Size: 191 1471
Fragments arranged by size:
1471 1135 984 681 437 248 219 191
118 16
SfoI GGC'GCC
Cuts at: 2734 2734
Size: 5500
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5500
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5500
SmaI CCC'GGG
Cuts at: 1872 2827 1872
Size: 955 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3604 4505 4767 820
Size: 197 926 1661 901 262 1553
Fragments arranged by size:
1661 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3291 493
Size: 2798 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5500
SphI G_CATG'C
Cuts at: 1132 2086 2858 1132
Size: 954 772 3774
Fragments arranged by size:
3774 954 772
SspI AAT'ATT
Cuts at: 5 52 3424 5
Size: 47 3372 2081
Fragments arranged by size:
3372 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5500
StyI C'CwwG_G
Cuts at: 513 1065 2004 2939 513
Size: 552 939 935 3074
Fragments arranged by size:
3074 939 935 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2238 3527 3544 3697 3882 4327 325
Size: 1255 658 1289 17 153 185 445 1498
Fragments arranged by size:
1498 1289 1255 658 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3746 5458 96
Size: 274 80 33 51 1443 1769 1712 138
Fragments arranged by size:
1769 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5460 5492 5460
Size: 32 5468
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5500
XbaI T'CTAG_A
Cuts at: 2836 2836
Size: 5500
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5500
XmaI C'CCGG_G
Cuts at: 1870 2825 1870
Size: 955 4545
XmnI GAAnn'nnTTC
Cuts at: 3629 3629
Size: 5500
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3308 276
Size: 53 83 186 936 1774 2468
Fragments arranged by size:
2468 1774 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NarI NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PshAI
PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI
EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI
PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI
SacII SanDI SapI SexAI SfiI SrfI SwaI XcmI