Restriction map for
pFN21AB4604
(Circular) (Six-base) MAPSORT of: pFN21AB4604.seq Check: 5589 from: 1 to: 5614
pFN21AB4604 5614 bp
With 182 enzymes: SgfI *
January 16, 2009 15:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3424 278
Size: 53 83 186 936 1888 2468
Fragments arranged by size:
2468 1888 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2957 1937
Size: 1020 4594
Acc65I G'GTAC_C
Cuts at: 2739 2935 2739
Size: 196 5418
AclI AA'CG_TT
Cuts at: 1563 3741 4114 1563
Size: 2178 373 3063
Fragments arranged by size:
3063 2178 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3677 5061 5329 1632
Size: 2045 1384 268 1917
Fragments arranged by size:
2045 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5417
AflIII A'CryG_T
Cuts at: 1203 2679 4513 5490 1203
Size: 1476 1834 977 1327
Fragments arranged by size:
1834 1476 1327 977
AgeI A'CCGG_T
Cuts at: 4445 4445
Size: 5614
AhdI GACnn_n'nnGTC
Cuts at: 4343 4343
Size: 5614
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5614
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5614
AlwNI CAG_nnn'CTG
Cuts at: 2584 3357 4929 2584
Size: 773 1572 3269
Fragments arranged by size:
3269 1572 773
ApaLI G'TGCA_C
Cuts at: 2392 3671 4827 2392
Size: 1279 1156 3179
Fragments arranged by size:
3179 1279 1156
ApoI r'AATT_y
Cuts at: 1493 2165 2489 2923 3149 3301 5410 1493
Size: 672 324 434 226 152 2109 1697
Fragments arranged by size:
2109 1697 672 434 324 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4168 160
Size: 872 3136 1606
Fragments arranged by size:
3136 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5614
AvaI C'yCGr_G
Cuts at: 1870 1943 2655 2939 1870
Size: 73 712 284 4545
Fragments arranged by size:
4545 712 284 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2109 2142 2109
Size: 33 5581
BamHI G'GATC_C
Cuts at: 2944 3311 2944
Size: 367 5247
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2405 2739 2935 4390 618
Size: 325 217 627 618 334 196 1455 1842
Fragments arranged by size:
1842 1455 627 618 334 325 217 196
BanII G_rGCy'C
Cuts at: 1391 2933 1391
Size: 1542 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4643
BbvCI CC'TCA_GC
Cuts at: 2061 2061
Size: 5614
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3805 3839 1903
Size: 34 1868 34 3678
Fragments arranged by size:
3678 1868 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3506 4716 3506
Size: 1210 4404
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5614
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2045 4225
Size: 107 122 71 631 605 337 36 2180
Cuts at: 4225 136
Size: 1525
Fragments arranged by size:
2180 1525 631 605 337 122 107 71
36
BglII A'GATC_T
Cuts at: 5609 5609
Size: 5614
BlpI GC'TnA_GC
Cuts at: 3031 3031
Size: 5614
Bme1580I G_kGCm'C
Cuts at: 1790 2396 2408 3675 4831 1790
Size: 606 12 1267 1156 2573
Fragments arranged by size:
2573 1267 1156 606 12
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5614
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3345 4303 442
Size: 416 53 83 399 24 1928 958 1753
Fragments arranged by size:
1928 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5614
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2420 2642 4274 1239
Size: 1181 222 1632 2579
Fragments arranged by size:
2579 1632 1181 222
Bpu10I CC'TnA_GC
Cuts at: 2061 2061
Size: 5614
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2501 3739 4604 4902 2501
Size: 1238 865 298 3213
Fragments arranged by size:
3213 1238 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2625 2810 4277 882
Size: 617 1126 185 1467 2219
Fragments arranged by size:
2219 1467 1126 617 185
BsaAI yAC'GTr
Cuts at: 493 1204 3405 493
Size: 711 2201 2702
Fragments arranged by size:
2702 2201 711
BsaBI GATnn'nnATC
Cuts at: 5608 5608
Size: 5614
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3421 3803 4438 275
Size: 53 83 186 936 1888 382 635 1451
Fragments arranged by size:
1888 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4047 4445 4719 4866 5277 4047
Size: 398 274 147 411 4384
Fragments arranged by size:
4384 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5389
Fragments arranged by size:
5389 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2210 1186
Size: 1024 4590
BseYI C'CCAG_C
Cuts at: 2308 4817 5313 2308
Size: 2509 496 2609
Fragments arranged by size:
2609 2509 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2199 2359 1163
Size: 573 463 160 4418
Fragments arranged by size:
4418 573 463 160
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3064 3825 4853 1540
Size: 275 186 1063 761 1028 2301
Fragments arranged by size:
2301 1063 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2396 2776 3675 3760 4831 2396
Size: 380 899 85 1071 3179
Fragments arranged by size:
3179 1071 899 380 85
BsmI GAATG_Cn'
Cuts at: 3125 3218 3125
Size: 93 5521
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2396 2408 2776 2933 3675 3760 4831
Size: 399 606 12 368 157 742 85 1071
Cuts at: 4831 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 606 399 368 157 85
12
BspEI T'CCGG_A
Cuts at: 5277 5277
Size: 5614
BspHI T'CATG_A
Cuts at: 3503 5233 3503
Size: 1730 3884
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2969 844
Size: 2125 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3501 1394
Size: 429 1678 3507
Fragments arranged by size:
3507 1678 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2350 2794 4109 4283 65
Size: 1183 1102 444 1315 174 1396
Fragments arranged by size:
1396 1315 1183 1102 444 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2864 4258 4445 1700
Size: 110 1054 1394 187 2869
Fragments arranged by size:
2869 1394 1054 187 110
BsrGI T'GTAC_A
Cuts at: 96 2682 2695 96
Size: 2586 13 3015
Fragments arranged by size:
3015 2586 13
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5614
BssSI C'ACGA_G
Cuts at: 3674 4686 3674
Size: 1012 4602
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2250 2584 794
Size: 1456 334 3824
Fragments arranged by size:
3824 1456 334
BstBI TT'CG_AA
Cuts at: 4418 4418
Size: 5614
BstEII G'GTnAC_C
Cuts at: 1184 2530 1184
Size: 1346 4268
BstYI r'GATC_y
Cuts at: 1972 2016 2598 2902 2944 3311 3696 3713 5165
Size: 44 582 304 42 367 385 17 1452
Cuts at: 5165 5251 5263 5609 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 582 385 367 346 304 86
44 42 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2640 2787 513
Size: 552 1575 147 3340
Fragments arranged by size:
3340 1575 552 147
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2830 487
Size: 683 56 1604 3271
Fragments arranged by size:
3271 1604 683 56
BtsI GCAGTG_nn'
Cuts at: 2230 2765 3130 3924 3944 2230
Size: 535 365 794 20 3900
Fragments arranged by size:
3900 794 535 365 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5614
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5579
DraI TTT'AAA
Cuts at: 2918 2918
Size: 5614
DraIII CAC_nnn'GTG
Cuts at: 2391 2641 2771 2391
Size: 250 130 5234
Fragments arranged by size:
5234 250 130
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4621 809
Size: 507 3305 1802
Fragments arranged by size:
3305 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2037 2710 3061
Size: 54 1425 50 275 18 207 673 351
Cuts at: 3061 3950 5579 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 673 351 275 207 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3061 1537
Size: 275 1249 4090
Fragments arranged by size:
4090 1249 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2187 2371 3544 1353
Size: 834 184 1173 3423
Fragments arranged by size:
3423 1173 834 184
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2222 4201 4575 4721 387
Size: 1064 138 633 1979 374 146 1280
Fragments arranged by size:
1979 1280 1064 633 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2420 2642 3677 4274 5061 5329 1239
Size: 393 788 222 1035 597 787 268 1524
Fragments arranged by size:
1524 1035 788 787 597 393 268 222
EcoO109I rG'GnC_Cy
Cuts at: 2010 2046 2293 2010
Size: 36 247 5331
Fragments arranged by size:
5331 247 36
EcoRI G'AATT_C
Cuts at: 2489 2923 3301 2489
Size: 434 378 4802
Fragments arranged by size:
4802 434 378
EcoRV GAT'ATC
Cuts at: 2893 2893
Size: 5614
FspI TGC'GCA
Cuts at: 2501 4120 2501
Size: 1619 3995
HaeII r_GCGC'y
Cuts at: 1678 2620 4761 1678
Size: 942 2141 2531
Fragments arranged by size:
2531 2141 942
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4255 4287 4329 4361 1373
Size: 32 135 32 2683 32 42 32 2626
Fragments arranged by size:
2683 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2958 3204 669
Size: 988 281 1020 246 3079
Fragments arranged by size:
3079 1020 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5614
HpaI GTT'AAC
Cuts at: 3204 3204
Size: 5614
KpnI G_GTAC'C
Cuts at: 2743 2939 2743
Size: 196 5418
MfeI C'AATT_G
Cuts at: 3213 3213
Size: 5614
MluI A'CGCG_T
Cuts at: 5490 5490
Size: 5614
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4728 4912 1305
Size: 3423 184 2007
Fragments arranged by size:
3423 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3792
Fragments arranged by size:
3792 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2276 2282 2607 2786 3572 3931 4090
Size: 648 1110 6 325 179 786 359 159
Cuts at: 4090 518
Size: 2042
Fragments arranged by size:
2042 1110 786 648 359 325 179 159
6
MspA1I CmG'CkG
Cuts at: 1606 2304 2716 3030 3707 4855 5100 1606
Size: 698 412 314 677 1148 245 2120
Fragments arranged by size:
2120 1148 698 677 412 314 245
NaeI GCC'GGC
Cuts at: 2866 2866
Size: 5614
NcoI C'CATG_G
Cuts at: 513 1065 2787 513
Size: 552 1722 3340
Fragments arranged by size:
3340 1722 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5614
NgoMIV G'CCGG_C
Cuts at: 2864 2864
Size: 5614
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5614
NotI GC'GGCC_GC
Cuts at: 3061 3061
Size: 5614
NspI r_CATG'y
Cuts at: 1132 1229 2506 2683 2972 4517 1132
Size: 97 1277 177 289 1545 2229
Fragments arranged by size:
2229 1545 1277 289 177 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5614
PciI A'CATG_T
Cuts at: 2679 4513 2679
Size: 1834 3780
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2641 1108
Size: 1533 4081
PmeI GTTT'AAAC
Cuts at: 2918 2918
Size: 5614
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3683 3715 5196 5228 3683
Size: 32 1481 32 4069
Fragments arranged by size:
4069 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2293 2293
Size: 5614
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5614
PsiI TTA'TAA
Cuts at: 3184 3184
Size: 5614
PstI C_TGCA'G
Cuts at: 830 2966 830
Size: 2136 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5614
PvuII CAG'CTG
Cuts at: 2304 2716 2304
Size: 412 5202
SalI G'TCGA_C
Cuts at: 1936 2956 1936
Size: 1020 4594
SbfI CC_TGCA'GG
Cuts at: 2966 2966
Size: 5614
ScaI AGT'ACT
Cuts at: 3862 5574 3862
Size: 1712 3902
SfcI C'TryA_G
Cuts at: 826 1045 2962 4097 4778 4969 826
Size: 219 1917 1135 681 191 1471
Fragments arranged by size:
1917 1471 1135 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2045 2045
Size: 5614
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5614
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5614
SmaI CCC'GGG
Cuts at: 1872 2657 2941 1872
Size: 785 284 4545
Fragments arranged by size:
4545 785 284
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2516 3718 4619 4881 820
Size: 197 926 573 1202 901 262 1553
Fragments arranged by size:
1553 1202 926 901 573 262 197
SnaBI TAC'GTA
Cuts at: 493 3405 493
Size: 2912 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5614
SphI G_CATG'C
Cuts at: 1132 2506 2972 1132
Size: 1374 466 3774
Fragments arranged by size:
3774 1374 466
SspI AAT'ATT
Cuts at: 5 52 3538 5
Size: 47 3486 2081
Fragments arranged by size:
3486 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5614
StyI C'CwwG_G
Cuts at: 513 1065 2049 2787 3053 513
Size: 552 984 738 266 3074
Fragments arranged by size:
3074 984 738 552 266
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2501 3641 3658 3811 3996 4441 325
Size: 1255 921 1140 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1140 921 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2682 2695 2841
Size: 274 80 33 51 1443 705 13 146
Cuts at: 2841 3860 5572 96
Size: 1019 1712 138
Fragments arranged by size:
1712 1443 1019 705 274 146 138 80
51 33 13
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5574 5606 5574
Size: 32 5582
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5614
XbaI T'CTAG_A
Cuts at: 2950 2950
Size: 5614
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5614
XmaI C'CCGG_G
Cuts at: 1870 2655 2939 1870
Size: 785 284 4545
Fragments arranged by size:
4545 785 284
XmnI GAAnn'nnTTC
Cuts at: 3743 3743
Size: 5614
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3422 276
Size: 53 83 186 936 1888 2468
Fragments arranged by size:
2468 1888 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI
SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BfrBI
BplI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoNI
FalI FseI FspAI KasI NarI NruI NsiI PacI
PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII
SanDI SapI SexAI SfoI SrfI SwaI XcmI