Restriction map for
pFN21AB4594
(Circular) (Six-base) MAPSORT of: pFN21AB4594.seq Check: 5315 from: 1 to: 6823
pFN21AB4594 6823 bp
With 182 enzymes: SgfI *
October 20, 2009 10:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3247 3957 3247
Size: 710 6113
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4633 278
Size: 53 83 186 936 3097 2468
Fragments arranged by size:
3097 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4166 1937
Size: 2229 4594
Acc65I G'GTAC_C
Cuts at: 4144 4144
Size: 6823
AclI AA'CG_TT
Cuts at: 1563 4950 5323 1563
Size: 3387 373 3063
Fragments arranged by size:
3387 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3914 3959 4886 6270 6538 1632
Size: 2282 45 927 1384 268 1917
Fragments arranged by size:
2282 1917 1384 927 268 45
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6626
AflIII A'CryG_T
Cuts at: 1203 5722 6699 1203
Size: 4519 977 1327
Fragments arranged by size:
4519 1327 977
AgeI A'CCGG_T
Cuts at: 3710 5654 3710
Size: 1944 4879
AhdI GACnn_n'nnGTC
Cuts at: 3280 5552 3280
Size: 2272 4551
AleI CACnn'nnGTG
Cuts at: 1166 2829 3855 3879 4067 1166
Size: 1663 1026 24 188 3922
Fragments arranged by size:
3922 1663 1026 188 24
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3484 1184
Size: 2300 4523
AlwNI CAG_nnn'CTG
Cuts at: 2720 3937 4091 4566 6138 2720
Size: 1217 154 475 1572 3405
Fragments arranged by size:
3405 1572 1217 475 154
ApaI G_GGCC'C
Cuts at: 3748 3748
Size: 6823
ApaLI G'TGCA_C
Cuts at: 3427 4880 6036 3427
Size: 1453 1156 4214
Fragments arranged by size:
4214 1453 1156
ApoI r'AATT_y
Cuts at: 1493 3786 4132 4358 4510 6619 1493
Size: 2293 346 226 152 2109 1697
Fragments arranged by size:
2293 2109 1697 346 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5377 160
Size: 872 4345 1606
Fragments arranged by size:
4345 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6823
AvaI C'yCGr_G
Cuts at: 1870 1943 4148 1870
Size: 73 2205 4545
Fragments arranged by size:
4545 2205 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2011 2044 2011
Size: 33 6790
BamHI G'GATC_C
Cuts at: 4153 4520 4153
Size: 367 6456
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3911 3924 4049 4144 5599
Size: 325 217 627 2124 13 125 95 1455
Cuts at: 5599 618
Size: 1842
Fragments arranged by size:
2124 1842 1455 627 325 217 125 95
13
BanII G_rGCy'C
Cuts at: 1391 2140 2958 3005 3104 3374 3748 4110 4142
Size: 749 818 47 99 270 374 362 32
Cuts at: 4142 1391
Size: 4072
Fragments arranged by size:
4072 818 749 374 362 270 99 47
32
BbeI G_GCGC'C
Cuts at: 4053 4053
Size: 6823
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5852
BbvCI CC'TCA_GC
Cuts at: 2838 2863 2838
Size: 25 6798
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5014 5048 1903
Size: 34 3077 34 3678
Fragments arranged by size:
3678 3077 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2039 4715 5925 2039
Size: 2676 1210 2937
Fragments arranged by size:
2937 2676 1210
BclI T'GATC_A
Cuts at: 1548 2217 1548
Size: 669 6154
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2015 3230 5434
Size: 107 122 71 631 605 343 1215 2204
Cuts at: 5434 136
Size: 1525
Fragments arranged by size:
2204 1525 1215 631 605 343 122 107
71
BglII A'GATC_T
Cuts at: 6818 6818
Size: 6823
BlpI GC'TnA_GC
Cuts at: 3198 4240 3198
Size: 1042 5781
Bme1580I G_kGCm'C
Cuts at: 1790 2692 3431 3748 3862 4884 6040 1790
Size: 902 739 317 114 1022 1156 2573
Fragments arranged by size:
2573 1156 1022 902 739 317 114
BmgBI CAC'GTC
Cuts at: 1318 3432 1318
Size: 2114 4709
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3511 3742 4554
Size: 416 53 83 399 24 2094 231 812
Cuts at: 4554 5512 442
Size: 958 1753
Fragments arranged by size:
2094 1753 958 812 416 399 231 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6823
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2938 3415 3613 2938
Size: 477 198 6148
Fragments arranged by size:
6148 477 198
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3056 3075 3263 3816 5483 1239
Size: 1817 19 188 553 1667 2579
Fragments arranged by size:
2579 1817 1667 553 188 19
Bpu10I CC'TnA_GC
Cuts at: 2838 2863 3189 2838
Size: 25 326 6472
Fragments arranged by size:
6472 326 25
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3587 4948 5813 6111 3587
Size: 1361 865 298 4299
Fragments arranged by size:
4299 1361 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2911 3506 5486 882
Size: 617 1412 595 1980 2219
Fragments arranged by size:
2219 1980 1412 617 595
BsaAI yAC'GTr
Cuts at: 493 1204 4614 493
Size: 711 3410 2702
Fragments arranged by size:
3410 2702 711
BsaBI GATnn'nnATC
Cuts at: 6817 6817
Size: 6823
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4050 4630 5012 5647
Size: 53 83 186 936 2517 580 382 635
Cuts at: 5647 275
Size: 1451
Fragments arranged by size:
2517 1451 936 635 580 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2079 2652 2976 3710 4102 5256 5654 5928 6075
Size: 573 324 734 392 1154 398 274 147
Cuts at: 6075 6486 2079
Size: 411 2416
Fragments arranged by size:
2416 1154 734 573 411 398 392 324
274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3358 3388 1178
Size: 30 165 30 1955 30 4613
Fragments arranged by size:
4613 1955 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2409 2527 2612 2651 2914 3515 3605 1186
Size: 1223 118 85 39 263 601 90 4404
Fragments arranged by size:
4404 1223 601 263 118 90 85 39
BseYI C'CCAG_C
Cuts at: 2549 3202 6026 6522 2549
Size: 653 2824 496 2850
Fragments arranged by size:
2850 2824 653 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6250
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2738 4273 5034 6062 1540
Size: 275 186 737 1535 761 1028 2301
Fragments arranged by size:
2301 1535 1028 761 737 275 186
BsiHKAI G_wGCw'C
Cuts at: 3153 3431 4110 4884 4969 6040 3153
Size: 278 679 774 85 1071 3936
Fragments arranged by size:
3936 1071 774 679 278 85
BsmI GAATG_Cn'
Cuts at: 3064 3175 4334 4427 3064
Size: 111 1159 93 5460
Fragments arranged by size:
5460 1159 111 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2081 2081
Size: 6823
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2140 2692 2958 3005 3104 3153 3374
Size: 399 350 552 266 47 99 49 221
Cuts at: 3374 3431 3748 3862 4110 4142 4884 4969 6040
Size: 57 317 114 248 32 742 85 1071
Cuts at: 6040 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 552 399 350 317 266
248 221 114 99 85 57 49 47
32
BspEI T'CCGG_A
Cuts at: 2079 2652 4102 6486 2079
Size: 573 1450 2384 2416
Fragments arranged by size:
2416 2384 1450 573
BspHI T'CATG_A
Cuts at: 4712 6442 4712
Size: 1730 5093
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2346 2511 2860 3247 3830 3957 4005 4104
Size: 1502 165 349 387 583 127 48 99
Cuts at: 4104 4178 844
Size: 74 3489
Fragments arranged by size:
3489 1502 583 387 349 165 127 99
74 48
BsrBI CCG'CTC
Cuts at: 1394 1823 2686 4710 1394
Size: 429 863 2024 3507
Fragments arranged by size:
3507 2024 863 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3571 5318 5492 65
Size: 1183 2323 1747 174 1396
Fragments arranged by size:
2323 1747 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2014 2733 3336 3710 3756 5467 5654
Size: 110 204 719 603 374 46 1711 187
Cuts at: 5654 1700
Size: 2869
Fragments arranged by size:
2869 1711 719 603 374 204 187 110
46
BsrGI T'GTAC_A
Cuts at: 96 2274 2556 2595 3838 96
Size: 2178 282 39 1243 3081
Fragments arranged by size:
3081 2178 1243 282 39
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6823
BssSI C'ACGA_G
Cuts at: 2421 3219 4883 5895 2421
Size: 798 1664 1012 3349
Fragments arranged by size:
3349 1664 1012 798
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3435 3616 794
Size: 2641 181 4001
Fragments arranged by size:
4001 2641 181
BstBI TT'CG_AA
Cuts at: 5627 5627
Size: 6823
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6823
BstXI CCAn_nnnn'nTGG
Cuts at: 3080 3482 4046 3080
Size: 402 564 5857
Fragments arranged by size:
5857 564 402
BstYI r'GATC_y
Cuts at: 1972 2227 4153 4520 4905 4922 6374 6460 6472
Size: 255 1926 367 385 17 1452 86 12
Cuts at: 6472 6818 1972
Size: 346 1977
Fragments arranged by size:
1977 1926 1452 385 367 346 255 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2244 3498 2244
Size: 1254 5569
BtgI C'CryG_G
Cuts at: 513 1065 2004 2325 3255 513
Size: 552 939 321 930 4081
Fragments arranged by size:
4081 939 930 552 321
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6084
Fragments arranged by size:
6084 683 56
BtsI GCAGTG_nn'
Cuts at: 2170 4339 5133 5153 2170
Size: 2169 794 20 3840
Fragments arranged by size:
3840 2169 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6823
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3721 3756 529
Size: 35 3157 35 3596
Fragments arranged by size:
3596 3157 35 35
DraI TTT'AAA
Cuts at: 4127 4127
Size: 6823
DraIII CAC_nnn'GTG
Cuts at: 2816 2816
Size: 6823
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5830 809
Size: 507 4514 1802
Fragments arranged by size:
4514 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2322 2664 2735
Size: 54 1425 50 275 18 492 342 71
Cuts at: 2735 3758 4270 5159 6788 8
Size: 1023 512 889 1629 43
Fragments arranged by size:
1629 1425 1023 889 512 492 342 275
71 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2735 4270 1537
Size: 275 923 1535 4090
Fragments arranged by size:
4090 1535 923 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2421 2630 2799 2907 3935 4753 1353
Size: 1068 209 169 108 1028 818 3423
Fragments arranged by size:
3423 1068 1028 818 209 169 108
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5410 5784 5930 387
Size: 1064 138 3821 374 146 1280
Fragments arranged by size:
3821 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 4108 4108
Size: 6823
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3056 3075 3263 3816 3914 3959 4886
Size: 393 1424 19 188 553 98 45 927
Cuts at: 4886 5483 6270 6538 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1424 927 787 597 553 393 268
188 98 45 19
EcoO109I rG'GnC_Cy
Cuts at: 2842 3185 3552 3744 2842
Size: 343 367 192 5921
Fragments arranged by size:
5921 367 343 192
EcoRI G'AATT_C
Cuts at: 3786 4132 4510 3786
Size: 346 378 6099
Fragments arranged by size:
6099 378 346
FspI TGC'GCA
Cuts at: 5329 5329
Size: 6823
HaeII r_GCGC'y
Cuts at: 1678 2520 4053 5970 1678
Size: 842 1533 1917 2531
Fragments arranged by size:
2531 1917 1533 842
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2241 2273 3266 3298 3358
Size: 32 135 32 669 32 993 32 60
Cuts at: 3358 3390 3503 3535 5464 5496 5538 5570 1373
Size: 32 113 32 1929 32 42 32 2626
Fragments arranged by size:
2626 1929 993 669 135 113 60 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3355 4167 4413 669
Size: 988 281 1417 812 246 3079
Fragments arranged by size:
3079 1417 988 812 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6823
HpaI GTT'AAC
Cuts at: 3355 4413 3355
Size: 1058 5765
KasI G'GCGC_C
Cuts at: 4049 4049
Size: 6823
KpnI G_GTAC'C
Cuts at: 4148 4148
Size: 6823
MfeI C'AATT_G
Cuts at: 4422 4422
Size: 6823
MluI A'CGCG_T
Cuts at: 6699 6699
Size: 6823
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5937 6121 1305
Size: 4632 184 2007
Fragments arranged by size:
4632 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2324 2666 10
Size: 54 1425 343 492 342 4167
Fragments arranged by size:
4167 1425 492 343 342 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2049 2114 2315 2330 2695 2829 3711
Size: 648 883 65 201 15 365 134 882
Cuts at: 3711 3855 3879 4067 4781 5140 5299 518
Size: 144 24 188 714 359 159 2042
Fragments arranged by size:
2042 883 882 714 648 365 359 201
188 159 144 134 65 24 15
MspA1I CmG'CkG
Cuts at: 1606 2025 3306 3482 3654 4021 4239 4916 6064
Size: 419 1281 176 172 367 218 677 1148
Cuts at: 6064 6309 1606
Size: 245 2120
Fragments arranged by size:
2120 1281 1148 677 419 367 245 218
176 172
NaeI GCC'GGC
Cuts at: 2016 2735 3338 2016
Size: 719 603 5501
Fragments arranged by size:
5501 719 603
NarI GG'CG_CC
Cuts at: 4050 4050
Size: 6823
NcoI C'CATG_G
Cuts at: 513 1065 2004 3255 513
Size: 552 939 1251 4081
Fragments arranged by size:
4081 1251 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6823
NgoMIV G'CCGG_C
Cuts at: 2014 2733 3336 2014
Size: 719 603 5501
Fragments arranged by size:
5501 719 603
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6823
NotI GC'GGCC_GC
Cuts at: 4270 4270
Size: 6823
NspI r_CATG'y
Cuts at: 1132 1229 2354 3415 3461 4181 5726 1132
Size: 97 1125 1061 46 720 1545 2229
Fragments arranged by size:
2229 1545 1125 1061 720 97 46
PasI CC'CwG_GG
Cuts at: 1348 2522 1348
Size: 1174 5649
PciI A'CATG_T
Cuts at: 5722 5722
Size: 6823
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2720 1108
Size: 1612 5211
PfoI T'CCnGG_A
Cuts at: 2262 2262
Size: 6823
PmeI GTTT'AAAC
Cuts at: 4127 4127
Size: 6823
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4892 4924 6405 6437 4892
Size: 32 1481 32 5278
Fragments arranged by size:
5278 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6823
PsiI TTA'TAA
Cuts at: 4393 4393
Size: 6823
PspOMI G'GGCC_C
Cuts at: 3744 3744
Size: 6823
PstI C_TGCA'G
Cuts at: 830 2165 2343 3050 3621 4175 830
Size: 1335 178 707 571 554 3478
Fragments arranged by size:
3478 1335 707 571 554 178
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6823
PvuII CAG'CTG
Cuts at: 2025 4021 2025
Size: 1996 4827
SacI G_AGCT'C
Cuts at: 4110 4110
Size: 6823
SalI G'TCGA_C
Cuts at: 1936 4165 1936
Size: 2229 4594
SapI GCTCTTCn'nnn_
Cuts at: 3935 3935
Size: 6823
SbfI CC_TGCA'GG
Cuts at: 4175 4175
Size: 6823
ScaI AGT'ACT
Cuts at: 3527 5071 6783 3527
Size: 1544 1712 3567
Fragments arranged by size:
3567 1712 1544
SfcI C'TryA_G
Cuts at: 826 1045 2161 2176 2339 2827 3046 3617 4171
Size: 219 1116 15 163 488 219 571 554
Cuts at: 4171 5306 5987 6178 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1135 1116 681 571 554 488 219
219 191 163 15
SfoI GGC'GCC
Cuts at: 4051 4051
Size: 6823
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6823
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6823
SmaI CCC'GGG
Cuts at: 1872 4150 1872
Size: 2278 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3602 4927 5828 6090 820
Size: 197 926 1659 1325 901 262 1553
Fragments arranged by size:
1659 1553 1325 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4614 493
Size: 4121 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6823
SphI G_CATG'C
Cuts at: 1132 3461 4181 1132
Size: 2329 720 3774
Fragments arranged by size:
3774 2329 720
SspI AAT'ATT
Cuts at: 5 52 4747 5
Size: 47 4695 2081
Fragments arranged by size:
4695 2081 47
StuI AGG'CCT
Cuts at: 1517 3596 3662 1517
Size: 2079 66 4678
Fragments arranged by size:
4678 2079 66
StyI C'CwwG_G
Cuts at: 513 1065 2004 2679 2787 3255 4065 4262 513
Size: 552 939 675 108 468 810 197 3074
Fragments arranged by size:
3074 939 810 675 552 468 197 108
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2514 2800 3268 4850 4867 5020 5205
Size: 1255 934 286 468 1582 17 153 185
Cuts at: 5205 5650 325
Size: 445 1498
Fragments arranged by size:
1582 1498 1255 934 468 445 286 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2274 2556 2595
Size: 274 80 33 51 1443 297 282 39
Cuts at: 2595 2727 3525 3838 5069 6781 96
Size: 132 798 313 1231 1712 138
Fragments arranged by size:
1712 1443 1231 798 313 297 282 274
138 132 80 51 39 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6783 6815 6783
Size: 32 6791
Tth111I GACn'n_nGTC
Cuts at: 1316 3517 1316
Size: 2201 4622
XbaI T'CTAG_A
Cuts at: 2224 4159 2224
Size: 1935 4888
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6823
XmaI C'CCGG_G
Cuts at: 1870 4148 1870
Size: 2278 4545
XmnI GAAnn'nnTTC
Cuts at: 4952 4952
Size: 6823
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4631 276
Size: 53 83 186 936 3097 2468
Fragments arranged by size:
3097 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BfrBI BsiWI BstZ17I EcoNI
EcoRV FalI FseI FspAI NruI NsiI PacI PmlI
PpuMI PspXI PsrI RsrII SacII SanDI SexAI SfiI
SrfI SwaI XcmI