Restriction map for
pFN21AB4593
(Circular) (Six-base) MAPSORT of: pFN21AB4593.seq Check: 340 from: 1 to: 7045
pFN21AB4593 7045 bp
With 182 enzymes: SgfI *
February 27, 2009 14:33 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4855 278
Size: 53 83 186 936 3319 2468
Fragments arranged by size:
3319 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2120 4388 1937
Size: 183 2268 4594
Fragments arranged by size:
4594 2268 183
Acc65I G'GTAC_C
Cuts at: 4366 4366
Size: 7045
AclI AA'CG_TT
Cuts at: 1563 2471 5172 5545 1563
Size: 908 2701 373 3063
Fragments arranged by size:
3063 2701 908 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3102 3678 3760 4164 4317 5108 6492 6760
Size: 1470 576 82 404 153 791 1384 268
Cuts at: 6760 1632
Size: 1917
Fragments arranged by size:
1917 1470 1384 791 576 404 268 153
82
AfeI AGC'GCT
Cuts at: 2438 2438
Size: 7045
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6848
AflIII A'CryG_T
Cuts at: 1203 3601 5944 6921 1203
Size: 2398 2343 977 1327
Fragments arranged by size:
2398 2343 1327 977
AgeI A'CCGG_T
Cuts at: 5876 5876
Size: 7045
AhdI GACnn_n'nnGTC
Cuts at: 5774 5774
Size: 7045
AleI CACnn'nnGTG
Cuts at: 1166 3140 1166
Size: 1974 5071
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7045
AlwNI CAG_nnn'CTG
Cuts at: 2159 3899 3992 4788 6360 2159
Size: 1740 93 796 1572 2844
Fragments arranged by size:
2844 1740 1572 796 93
ApaLI G'TGCA_C
Cuts at: 5102 6258 5102
Size: 1156 5889
ApoI r'AATT_y
Cuts at: 1493 2030 2278 2408 2487 2546 2749 2780 2910
Size: 537 248 130 79 59 203 31 130
Cuts at: 2910 3301 3481 3550 4354 4580 4732 6841 1493
Size: 391 180 69 804 226 152 2109 1697
Fragments arranged by size:
2109 1697 804 537 391 248 226 203
180 152 130 130 79 69 59 31
AseI AT'TA_AT
Cuts at: 160 1032 5599 160
Size: 872 4567 1606
Fragments arranged by size:
4567 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7045
AvaI C'yCGr_G
Cuts at: 1870 1943 2091 4370 1870
Size: 73 148 2279 4545
Fragments arranged by size:
4545 2279 148 73
BamHI G'GATC_C
Cuts at: 4375 4742 4375
Size: 367 6678
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2720 4366 5821 618
Size: 325 217 627 933 1646 1455 1842
Fragments arranged by size:
1842 1646 1455 933 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2923 4364 1391
Size: 1532 1441 4072
Fragments arranged by size:
4072 1532 1441
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2466 928
Size: 971 567 5507
Fragments arranged by size:
5507 971 567
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5236 5270 1903
Size: 34 3299 34 3678
Fragments arranged by size:
3678 3299 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4937 6147 4937
Size: 1210 5835
BclI T'GATC_A
Cuts at: 1548 2215 2385 4177 1548
Size: 667 170 1792 4416
Fragments arranged by size:
4416 1792 667 170
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5656 136
Size: 107 122 71 631 605 3984 1525
Fragments arranged by size:
3984 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3454 7040 3454
Size: 3586 3459
BlpI GC'TnA_GC
Cuts at: 4462 4462
Size: 7045
Bme1580I G_kGCm'C
Cuts at: 1790 5106 6262 1790
Size: 3316 1156 2573
Fragments arranged by size:
3316 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7045
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4776 5734 442
Size: 416 53 83 399 24 3359 958 1753
Fragments arranged by size:
3359 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7045
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2181 5705 1239
Size: 942 3524 2579
Fragments arranged by size:
3524 2579 942
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2307 5170 6035 6333 2307
Size: 2863 865 298 3019
Fragments arranged by size:
3019 2863 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5708 882
Size: 617 4209 2219
Fragments arranged by size:
4209 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2745 4836 493
Size: 711 1541 2091 2702
Fragments arranged by size:
2702 2091 1541 711
BsaBI GATnn'nnATC
Cuts at: 7039 7039
Size: 7045
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2292 4852 5234 5869
Size: 53 83 186 936 759 2560 382 635
Cuts at: 5869 275
Size: 1451
Fragments arranged by size:
2560 1451 936 759 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3073 5478 5876 6150 6297 6708 3073
Size: 2405 398 274 147 411 3410
Fragments arranged by size:
3410 2405 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3094 3124 1178
Size: 30 165 30 1691 30 5099
Fragments arranged by size:
5099 1691 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2991 2994 4017 1186
Size: 1805 3 1023 4214
Fragments arranged by size:
4214 1805 1023 3
BseYI C'CCAG_C
Cuts at: 2762 6248 6744 2762
Size: 3486 496 3063
Fragments arranged by size:
3486 3063 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2721 1163
Size: 573 985 5487
Fragments arranged by size:
5487 985 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4495 5256 6284 1540
Size: 275 186 2494 761 1028 2301
Fragments arranged by size:
2494 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2923 5106 5191 6262 2923
Size: 2183 85 1071 3706
Fragments arranged by size:
3706 2183 1071 85
BsmI GAATG_Cn'
Cuts at: 2824 4556 4649 2824
Size: 1732 93 5220
Fragments arranged by size:
5220 1732 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2923 4364 5106 5191 6262 1391
Size: 399 1133 1441 742 85 1071 2174
Fragments arranged by size:
2174 1441 1133 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 6708 6708
Size: 7045
BspHI T'CATG_A
Cuts at: 3754 4934 6664 3754
Size: 1180 1730 4135
Fragments arranged by size:
4135 1730 1180
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4400 844
Size: 3556 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4932 1394
Size: 429 3109 3507
Fragments arranged by size:
3507 3109 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2692 3951 5540 5714 65
Size: 1183 1444 1259 1589 174 1396
Fragments arranged by size:
1589 1444 1396 1259 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5689 5876 1700
Size: 110 3879 187 2869
Fragments arranged by size:
3879 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7045
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7045
BssSI C'ACGA_G
Cuts at: 5105 6117 5105
Size: 1012 6033
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7045
BstBI TT'CG_AA
Cuts at: 5849 5849
Size: 7045
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7045
BstYI r'GATC_y
Cuts at: 1972 2019 3454 4324 4375 4742 5127 5144 6596
Size: 47 1435 870 51 367 385 17 1452
Cuts at: 6596 6682 6694 7040 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1435 870 385 367 346 86
51 47 17 12
Bsu36I CC'TnA_GG
Cuts at: 2024 2024
Size: 7045
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5554
Fragments arranged by size:
5554 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6306
Fragments arranged by size:
6306 683 56
BtsI GCAGTG_nn'
Cuts at: 2704 3505 4561 5355 5375 2704
Size: 801 1056 794 20 4374
Fragments arranged by size:
4374 1056 801 794 20
ClaI AT'CG_AT
Cuts at: 1554 4297 1554
Size: 2743 4302
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3081 3116 4264 4299 529
Size: 35 2517 35 1148 35 3275
Fragments arranged by size:
3275 2517 1148 35 35 35
DraI TTT'AAA
Cuts at: 2485 4349 2485
Size: 1864 5181
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6052 809
Size: 507 4736 1802
Fragments arranged by size:
4736 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2594 4492 5381
Size: 54 1425 50 275 18 764 1898 889
Cuts at: 5381 7010 8
Size: 1629 43
Fragments arranged by size:
1898 1629 1425 889 764 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4492 1537
Size: 275 2680 4090
Fragments arranged by size:
4090 2680 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2051 3779 4975 1353
Size: 698 1728 1196 3423
Fragments arranged by size:
3423 1728 1196 698
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5632 6006 6152 387
Size: 1064 138 4043 374 146 1280
Fragments arranged by size:
4043 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2921 2921
Size: 7045
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2181 3102 3678 3760 4164 4317 5108
Size: 393 549 921 576 82 404 153 791
Cuts at: 5108 5705 6492 6760 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 921 791 787 597 576 549 404
393 268 153 82
EcoO109I rG'GnC_Cy
Cuts at: 2327 3021 3325 2327
Size: 694 304 6047
Fragments arranged by size:
6047 694 304
EcoRI G'AATT_C
Cuts at: 3481 3550 4354 4732 3481
Size: 69 804 378 5794
Fragments arranged by size:
5794 804 378 69
EcoRV GAT'ATC
Cuts at: 3676 3676
Size: 7045
FspI TGC'GCA
Cuts at: 5551 5551
Size: 7045
HaeII r_GCGC'y
Cuts at: 1678 2440 6192 1678
Size: 762 3752 2531
Fragments arranged by size:
3752 2531 762
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2644 2676 3094 3126 3413
Size: 32 135 32 1072 32 418 32 287
Cuts at: 3413 3445 3809 3841 5686 5718 5760 5792 1373
Size: 32 364 32 1845 32 42 32 2626
Fragments arranged by size:
2626 1845 1072 418 364 287 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4389 4635 669
Size: 988 281 2451 246 3079
Fragments arranged by size:
3079 2451 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2644 748
Size: 1896 5149
HpaI GTT'AAC
Cuts at: 4635 4635
Size: 7045
KpnI G_GTAC'C
Cuts at: 4370 4370
Size: 7045
MfeI C'AATT_G
Cuts at: 3270 4644 3270
Size: 1374 5671
MluI A'CGCG_T
Cuts at: 6921 6921
Size: 7045
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3152 4289 6159 6343 1305
Size: 1847 1137 1870 184 2007
Fragments arranged by size:
2007 1870 1847 1137 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2596 10
Size: 54 1425 343 764 4459
Fragments arranged by size:
4459 1425 764 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2393 2673 2697 3140 3813 3987 5003
Size: 648 1227 280 24 443 673 174 1016
Cuts at: 5003 5362 5521 518
Size: 359 159 2042
Fragments arranged by size:
2042 1227 1016 673 648 443 359 280
174 159 24
MspA1I CmG'CkG
Cuts at: 1606 4187 4461 5138 6286 6531 1606
Size: 2581 274 677 1148 245 2120
Fragments arranged by size:
2581 2120 1148 677 274 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5554
Fragments arranged by size:
5554 939 552
NdeI CA'TA_TG
Cuts at: 387 2311 3186 387
Size: 1924 875 4246
Fragments arranged by size:
4246 1924 875
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7045
NotI GC'GGCC_GC
Cuts at: 4492 4492
Size: 7045
NspI r_CATG'y
Cuts at: 1132 1229 3386 3605 4403 5948 1132
Size: 97 2157 219 798 1545 2229
Fragments arranged by size:
2229 2157 1545 798 219 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7045
PciI A'CATG_T
Cuts at: 3601 5944 3601
Size: 2343 4702
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7045
PmeI GTTT'AAAC
Cuts at: 4349 4349
Size: 7045
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5114 5146 6627 6659 5114
Size: 32 1481 32 5500
Fragments arranged by size:
5500 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2327 3021 3325 2327
Size: 694 304 6047
Fragments arranged by size:
6047 694 304
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7045
PsiI TTA'TAA
Cuts at: 2285 4615 2285
Size: 2330 4715
PstI C_TGCA'G
Cuts at: 830 2833 3069 3500 4397 830
Size: 2003 236 431 897 3478
Fragments arranged by size:
3478 2003 897 431 236
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7045
SacI G_AGCT'C
Cuts at: 2923 2923
Size: 7045
SalI G'TCGA_C
Cuts at: 1936 4387 1936
Size: 2451 4594
SbfI CC_TGCA'GG
Cuts at: 4397 4397
Size: 7045
ScaI AGT'ACT
Cuts at: 3913 5293 7005 3913
Size: 1380 1712 3953
Fragments arranged by size:
3953 1712 1380
SfcI C'TryA_G
Cuts at: 826 1045 2829 3065 3229 3496 4393 5528 6209
Size: 219 1784 236 164 267 897 1135 681
Cuts at: 6209 6400 826
Size: 191 1471
Fragments arranged by size:
1784 1471 1135 897 681 267 236 219
191 164
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7045
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7045
SmaI CCC'GGG
Cuts at: 1872 2093 4372 1872
Size: 221 2279 4545
Fragments arranged by size:
4545 2279 221
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2322 5149 6050 6312 820
Size: 197 926 379 2827 901 262 1553
Fragments arranged by size:
2827 1553 926 901 379 262 197
SnaBI TAC'GTA
Cuts at: 493 4836 493
Size: 4343 2702
SpeI A'CTAG_T
Cuts at: 152 3908 152
Size: 3756 3289
SphI G_CATG'C
Cuts at: 1132 4403 1132
Size: 3271 3774
SspI AAT'ATT
Cuts at: 5 52 3874 4969 5
Size: 47 3822 1095 2081
Fragments arranged by size:
3822 2081 1095 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7045
StyI C'CwwG_G
Cuts at: 513 1065 2004 3017 3566 4484 513
Size: 552 939 1013 549 918 3074
Fragments arranged by size:
3074 1013 939 918 552 549
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3109 5072 5089 5242 5427 5872 325
Size: 1255 1529 1963 17 153 185 445 1498
Fragments arranged by size:
1963 1529 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2833 3911 5291
Size: 274 80 33 51 1443 856 1078 1380
Cuts at: 5291 7003 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1380 1078 856 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2416 2448 7005 7037 2416
Size: 32 4557 32 2424
Fragments arranged by size:
4557 2424 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7045
XbaI T'CTAG_A
Cuts at: 4381 4381
Size: 7045
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 4335 2011
Size: 2324 4721
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7045
XmaI C'CCGG_G
Cuts at: 1870 2091 4370 1870
Size: 221 2279 4545
Fragments arranged by size:
4545 2279 221
XmnI GAAnn'nnTTC
Cuts at: 2676 2859 5174 2676
Size: 183 2315 4547
Fragments arranged by size:
4547 2315 183
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4853 276
Size: 53 83 186 936 3319 2468
Fragments arranged by size:
3319 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI SacI SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII
EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI
PvuII RsrII SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI