Restriction map for
pFN21AB4590
(Circular) (Six-base) MAPSORT of: pFN21AB4590.seq Check: 2967 from: 1 to: 6724
pFN21AB4590 6724 bp
With 182 enzymes: SgfI *
February 27, 2009 14:33 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4534 278
Size: 53 83 186 936 2998 2468
Fragments arranged by size:
2998 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2179 4067 1937
Size: 242 1888 4594
Fragments arranged by size:
4594 1888 242
Acc65I G'GTAC_C
Cuts at: 4045 4045
Size: 6724
AclI AA'CG_TT
Cuts at: 1563 4851 5224 1563
Size: 3288 373 3063
Fragments arranged by size:
3288 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2034 2052 2412 2933 2940 4787 6171 6439
Size: 402 18 360 521 7 1847 1384 268
Cuts at: 6439 1632
Size: 1917
Fragments arranged by size:
1917 1847 1384 521 402 360 268 18
7
AflII C'TTAA_G
Cuts at: 820 1017 2249 820
Size: 197 1232 5295
Fragments arranged by size:
5295 1232 197
AflIII A'CryG_T
Cuts at: 1203 3963 5623 6600 1203
Size: 2760 1660 977 1327
Fragments arranged by size:
2760 1660 1327 977
AgeI A'CCGG_T
Cuts at: 5555 5555
Size: 6724
AhdI GACnn_n'nnGTC
Cuts at: 5453 5453
Size: 6724
AleI CACnn'nnGTG
Cuts at: 1166 3977 1166
Size: 2811 3913
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2982 1184
Size: 1798 4926
AlwNI CAG_nnn'CTG
Cuts at: 3922 4467 6039 3922
Size: 545 1572 4607
Fragments arranged by size:
4607 1572 545
ApaLI G'TGCA_C
Cuts at: 4781 5937 4781
Size: 1156 5568
ApoI r'AATT_y
Cuts at: 1493 3083 3345 3545 3852 4033 4259 4411 6520
Size: 1590 262 200 307 181 226 152 2109
Cuts at: 6520 1493
Size: 1697
Fragments arranged by size:
2109 1697 1590 307 262 226 200 181
152
AseI AT'TA_AT
Cuts at: 160 1032 5278 160
Size: 872 4246 1606
Fragments arranged by size:
4246 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6724
AvaI C'yCGr_G
Cuts at: 1870 1943 4049 1870
Size: 73 2106 4545
Fragments arranged by size:
4545 2106 73
BamHI G'GATC_C
Cuts at: 3014 4054 4421 3014
Size: 1040 367 5317
Fragments arranged by size:
5317 1040 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2684 4045 5500 618
Size: 325 217 627 897 1361 1455 1842
Fragments arranged by size:
1842 1455 1361 897 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3667 4043 1391
Size: 2276 376 4072
Fragments arranged by size:
4072 2276 376
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2109 2556 2651 928
Size: 971 210 447 95 5001
Fragments arranged by size:
5001 971 447 210 95
BbvCI CC'TCA_GC
Cuts at: 4008 4008
Size: 6724
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4915 4949 1903
Size: 34 2978 34 3678
Fragments arranged by size:
3678 2978 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4616 5826 4616
Size: 1210 5514
BclI T'GATC_A
Cuts at: 1548 3885 3907 1548
Size: 2337 22 4365
Fragments arranged by size:
4365 2337 22
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5335 136
Size: 107 122 71 631 605 3663 1525
Fragments arranged by size:
3663 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6719 6719
Size: 6724
BlpI GC'TnA_GC
Cuts at: 4141 4141
Size: 6724
Bme1580I G_kGCm'C
Cuts at: 1790 4785 5941 1790
Size: 2995 1156 2573
Fragments arranged by size:
2995 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6724
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3175 4455 5413
Size: 416 53 83 399 24 1758 1280 958
Cuts at: 5413 442
Size: 1753
Fragments arranged by size:
1758 1753 1280 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6724
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2726 3486 3631 5384 1239
Size: 1487 760 145 1753 2579
Fragments arranged by size:
2579 1753 1487 760 145
Bpu10I CC'TnA_GC
Cuts at: 2379 2805 3927 4008 2379
Size: 426 1122 81 5095
Fragments arranged by size:
5095 1122 426 81
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4849 5714 6012 4849
Size: 865 298 5561
Fragments arranged by size:
5561 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2576 3649 5387 882
Size: 617 1077 1073 1738 2219
Fragments arranged by size:
2219 1738 1077 1073 617
BsaAI yAC'GTr
Cuts at: 493 1204 4515 493
Size: 711 3311 2702
Fragments arranged by size:
3311 2702 711
BsaBI GATnn'nnATC
Cuts at: 3906 6718 3906
Size: 2812 3912
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4531 4913 5548 275
Size: 53 83 186 936 2998 382 635 1451
Fragments arranged by size:
2998 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5157 5555 5829 5976 6387 5157
Size: 398 274 147 411 5494
Fragments arranged by size:
5494 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6499
Fragments arranged by size:
6499 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2144 2307 2580 1186
Size: 958 163 273 5330
Fragments arranged by size:
5330 958 273 163
BseYI C'CCAG_C
Cuts at: 2145 3265 3716 5927 6423 2145
Size: 1120 451 2211 496 2446
Fragments arranged by size:
2446 2211 1120 496 451
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2615 3122 1163
Size: 573 879 507 4765
Fragments arranged by size:
4765 879 573 507
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4174 4935 5963 1540
Size: 275 186 2173 761 1028 2301
Fragments arranged by size:
2301 2173 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3667 4785 4870 5941 3667
Size: 1118 85 1071 4450
Fragments arranged by size:
4450 1118 1071 85
BsmI GAATG_Cn'
Cuts at: 4235 4328 4235
Size: 93 6631
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3667 4043 4785 4870 5941 1391
Size: 399 1877 376 742 85 1071 2174
Fragments arranged by size:
2174 1877 1071 742 399 376 85
BspEI T'CCGG_A
Cuts at: 6387 6387
Size: 6724
BspHI T'CATG_A
Cuts at: 3910 4613 6343 3910
Size: 703 1730 4291
Fragments arranged by size:
4291 1730 703
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4079 844
Size: 3235 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4611 1394
Size: 429 2788 3507
Fragments arranged by size:
3507 2788 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2964 3753 5219 5393 65
Size: 1183 1716 789 1466 174 1396
Fragments arranged by size:
1716 1466 1396 1183 789 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3385 5368 5555 1700
Size: 110 1575 1983 187 2869
Fragments arranged by size:
2869 1983 1575 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6724
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6724
BssSI C'ACGA_G
Cuts at: 4784 5796 4784
Size: 1012 5712
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6724
BstBI TT'CG_AA
Cuts at: 5528 5528
Size: 6724
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6724
BstYI r'GATC_y
Cuts at: 1972 2824 3014 4054 4421 4806 4823 6275 6361
Size: 852 190 1040 367 385 17 1452 86
Cuts at: 6361 6373 6719 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1040 852 385 367 346 190
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2243 2864 3759 513
Size: 552 1178 621 895 3478
Fragments arranged by size:
3478 1178 895 621 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5985
Fragments arranged by size:
5985 683 56
BtsI GCAGTG_nn'
Cuts at: 4240 5034 5054 4240
Size: 794 20 5910
Fragments arranged by size:
5910 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6724
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3963 3998 529
Size: 35 3399 35 3255
Fragments arranged by size:
3399 3255 35 35
DraI TTT'AAA
Cuts at: 4028 4028
Size: 6724
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3538 3968 5731 809
Size: 507 2222 430 1763 1802
Fragments arranged by size:
2222 1802 1763 507 430
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2280 2974 3329
Size: 54 1425 50 275 18 450 694 355
Cuts at: 3329 3559 4171 5060 6689 8
Size: 230 612 889 1629 43
Fragments arranged by size:
1629 1425 889 694 612 450 355 275
230 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4171 1537
Size: 275 2359 4090
Fragments arranged by size:
4090 2359 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2186 2520 3114 4654 1353
Size: 833 334 594 1540 3423
Fragments arranged by size:
3423 1540 833 594 334
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5311 5685 5831 387
Size: 1064 138 3722 374 146 1280
Fragments arranged by size:
3722 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3665 3665
Size: 6724
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2034 2052 2412 2726 2933 2940 3486
Size: 393 402 18 360 314 207 7 546
Cuts at: 3486 3631 4787 5384 6171 6439 1239
Size: 145 1156 597 787 268 1524
Fragments arranged by size:
1524 1156 787 597 546 402 393 360
314 268 207 145 18 7
EcoRI G'AATT_C
Cuts at: 3083 4033 4411 3083
Size: 950 378 5396
Fragments arranged by size:
5396 950 378
EcoRV GAT'ATC
Cuts at: 2926 2926
Size: 6724
FspI TGC'GCA
Cuts at: 5230 5230
Size: 6724
HaeII r_GCGC'y
Cuts at: 1678 3714 5871 1678
Size: 2036 2157 2531
Fragments arranged by size:
2531 2157 2036
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2609 2641 2954 2986 2989
Size: 32 135 32 1037 32 313 32 3
Cuts at: 2989 3021 3297 3329 5365 5397 5439 5471 1373
Size: 32 276 32 2036 32 42 32 2626
Fragments arranged by size:
2626 2036 1037 313 276 135 42 32
32 32 32 32 32 32 32 3
HincII GTy'rAC
Cuts at: 669 1657 1938 3223 4068 4314 669
Size: 988 281 1285 845 246 3079
Fragments arranged by size:
3079 1285 988 845 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6724
HpaI GTT'AAC
Cuts at: 4314 4314
Size: 6724
KpnI G_GTAC'C
Cuts at: 4049 4049
Size: 6724
MfeI C'AATT_G
Cuts at: 2912 4323 2912
Size: 1411 5313
MluI A'CGCG_T
Cuts at: 6600 6600
Size: 6724
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5838 6022 1305
Size: 4533 184 2007
Fragments arranged by size:
4533 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2282 10
Size: 54 1425 343 450 4452
Fragments arranged by size:
4452 1425 450 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3758 3977 4682 5041 5200 518
Size: 648 2592 219 705 359 159 2042
Fragments arranged by size:
2592 2042 705 648 359 219 159
MspA1I CmG'CkG
Cuts at: 1606 2162 2697 4140 4817 5965 6210 1606
Size: 556 535 1443 677 1148 245 2120
Fragments arranged by size:
2120 1443 1148 677 556 535 245
NcoI C'CATG_G
Cuts at: 513 1065 2243 2864 513
Size: 552 1178 621 4373
Fragments arranged by size:
4373 1178 621 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6724
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6724
NotI GC'GGCC_GC
Cuts at: 4171 4171
Size: 6724
NspI r_CATG'y
Cuts at: 1132 1229 2717 3967 4082 5627 1132
Size: 97 1488 1250 115 1545 2229
Fragments arranged by size:
2229 1545 1488 1250 115 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6724
PciI A'CATG_T
Cuts at: 3963 5623 3963
Size: 1660 5064
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3922 1108
Size: 2814 3910
PfoI T'CCnGG_A
Cuts at: 3647 3647
Size: 6724
PmeI GTTT'AAAC
Cuts at: 4028 4028
Size: 6724
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4793 4825 6306 6338 4793
Size: 32 1481 32 5179
Fragments arranged by size:
5179 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 3687 1613
Size: 2074 4650
PsiI TTA'TAA
Cuts at: 4294 4294
Size: 6724
PstI C_TGCA'G
Cuts at: 830 2161 3030 4076 830
Size: 1331 869 1046 3478
Fragments arranged by size:
3478 1331 1046 869
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6724
PvuII CAG'CTG
Cuts at: 2162 2697 2162
Size: 535 6189
SacI G_AGCT'C
Cuts at: 3667 3667
Size: 6724
SalI G'TCGA_C
Cuts at: 1936 4066 1936
Size: 2130 4594
SbfI CC_TGCA'GG
Cuts at: 3030 4076 3030
Size: 1046 5678
ScaI AGT'ACT
Cuts at: 4972 6684 4972
Size: 1712 5012
SfcI C'TryA_G
Cuts at: 826 1045 2157 3026 3487 3553 4072 5207 5888
Size: 219 1112 869 461 66 519 1135 681
Cuts at: 5888 6079 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1112 869 681 519 461 219
191 66
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6724
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6724
SmaI CCC'GGG
Cuts at: 1872 4051 1872
Size: 2179 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2249 4828 5729 5991 820
Size: 197 926 306 2579 901 262 1553
Fragments arranged by size:
2579 1553 926 901 306 262 197
SnaBI TAC'GTA
Cuts at: 493 4515 493
Size: 4022 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6724
SphI G_CATG'C
Cuts at: 1132 4082 1132
Size: 2950 3774
SspI AAT'ATT
Cuts at: 5 52 4648 5
Size: 47 4596 2081
Fragments arranged by size:
4596 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6724
StyI C'CwwG_G
Cuts at: 513 1065 2073 2243 2586 2718 2864 3237 3534
Size: 552 1008 170 343 132 146 373 297
Cuts at: 3534 4163 513
Size: 629 3074
Fragments arranged by size:
3074 1008 629 552 373 343 297 170
146 132
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3581 3958 4751 4768 4921 5106 5551
Size: 1255 2001 377 793 17 153 185 445
Cuts at: 5551 325
Size: 1498
Fragments arranged by size:
2001 1498 1255 793 445 377 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4970 6682 96
Size: 274 80 33 51 1443 2993 1712 138
Fragments arranged by size:
2993 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6684 6716 6684
Size: 32 6692
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6724
XbaI T'CTAG_A
Cuts at: 3655 4060 3655
Size: 405 6319
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6724
XmaI C'CCGG_G
Cuts at: 1870 4049 1870
Size: 2179 4545
XmnI GAAnn'nnTTC
Cuts at: 2355 4853 2355
Size: 2498 4226
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4532 276
Size: 53 83 186 936 2998 2468
Fragments arranged by size:
2998 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoRI EcoRV FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI
PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoNI EcoO109I FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI
PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI XcmI