Restriction map for
pFN21AB4582
(Circular) (Six-base) MAPSORT of: pFN21AB4582.seq Check: 3974 from: 1 to: 6589
pFN21AB4582 6589 bp
With 182 enzymes: SgfI *
February 27, 2009 14:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2168 2280 2168
Size: 112 6477
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4399 278
Size: 53 83 186 936 2863 2468
Fragments arranged by size:
2863 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3343 3932 1937
Size: 1406 589 4594
Fragments arranged by size:
4594 1406 589
Acc65I G'GTAC_C
Cuts at: 3910 3910
Size: 6589
AclI AA'CG_TT
Cuts at: 1563 4716 5089 1563
Size: 3153 373 3063
Fragments arranged by size:
3153 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2681 2818 3695 4652 6036 6304 1632
Size: 1049 137 877 957 1384 268 1917
Fragments arranged by size:
1917 1384 1049 957 877 268 137
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6392
AflIII A'CryG_T
Cuts at: 1203 5488 6465 1203
Size: 4285 977 1327
Fragments arranged by size:
4285 1327 977
AgeI A'CCGG_T
Cuts at: 5420 5420
Size: 6589
AhdI GACnn_n'nnGTC
Cuts at: 5318 5318
Size: 6589
AleI CACnn'nnGTG
Cuts at: 1166 2048 1166
Size: 882 5707
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6589
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3688 3720 3688
Size: 32 6557
AlwNI CAG_nnn'CTG
Cuts at: 2689 2913 2997 4332 5904 2689
Size: 224 84 1335 1572 3374
Fragments arranged by size:
3374 1572 1335 224 84
ApaLI G'TGCA_C
Cuts at: 2339 3446 4646 5802 2339
Size: 1107 1200 1156 3126
Fragments arranged by size:
3126 1200 1156 1107
ApoI r'AATT_y
Cuts at: 1493 2122 2375 3898 4124 4276 6385 1493
Size: 629 253 1523 226 152 2109 1697
Fragments arranged by size:
2109 1697 1523 629 253 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5143 160
Size: 872 4111 1606
Fragments arranged by size:
4111 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6589
AvaI C'yCGr_G
Cuts at: 1870 1943 3914 1870
Size: 73 1971 4545
Fragments arranged by size:
4545 1971 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3612 3645 3612
Size: 33 6556
BamHI G'GATC_C
Cuts at: 3919 4286 3919
Size: 367 6222
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3910 5365 618
Size: 325 217 627 2123 1455 1842
Fragments arranged by size:
2123 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2980 3908 1391
Size: 1589 928 4072
Fragments arranged by size:
4072 1589 928
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5618
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4780 4814 1903
Size: 34 2843 34 3678
Fragments arranged by size:
3678 2843 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3308 4481 5691 3308
Size: 1173 1210 4206
Fragments arranged by size:
4206 1210 1173
BclI T'GATC_A
Cuts at: 1548 2809 1548
Size: 1261 5328
BfrBI ATG'CAT
Cuts at: 2817 2817
Size: 6589
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5200 136
Size: 107 122 71 631 605 3528 1525
Fragments arranged by size:
3528 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6584 6584
Size: 6589
BlpI GC'TnA_GC
Cuts at: 4006 4006
Size: 6589
Bme1580I G_kGCm'C
Cuts at: 1790 2343 2929 3450 4650 5806 1790
Size: 553 586 521 1200 1156 2573
Fragments arranged by size:
2573 1200 1156 586 553 521
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6589
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3328 4320 5278
Size: 416 53 83 399 24 1911 992 958
Cuts at: 5278 442
Size: 1753
Fragments arranged by size:
1911 1753 992 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6589
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2097 2545 2601 3153 5249 1239
Size: 858 448 56 552 2096 2579
Fragments arranged by size:
2579 2096 858 552 448 56
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4714 5579 5877 4714
Size: 865 298 5426
Fragments arranged by size:
5426 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3148 5252 882
Size: 617 1649 2104 2219
Fragments arranged by size:
2219 2104 1649 617
BsaAI yAC'GTr
Cuts at: 493 1204 4380 493
Size: 711 3176 2702
Fragments arranged by size:
3176 2702 711
BsaBI GATnn'nnATC
Cuts at: 6583 6583
Size: 6589
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4396 4778 5413 275
Size: 53 83 186 936 2863 382 635 1451
Fragments arranged by size:
2863 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2301 3380 5022 5420 5694 5841 6252 2301
Size: 1079 1642 398 274 147 411 2638
Fragments arranged by size:
2638 1642 1079 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2574 2604 2962 2992 1178
Size: 30 165 30 1171 30 358 30 4775
Fragments arranged by size:
4775 1171 358 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2598 1186
Size: 1412 5177
BseYI C'CCAG_C
Cuts at: 3248 5792 6288 3248
Size: 2544 496 3549
Fragments arranged by size:
3549 2544 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2026 2702 3010 3483 1163
Size: 573 290 676 308 473 4269
Fragments arranged by size:
4269 676 573 473 308 290
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4039 4800 5828 1540
Size: 275 186 2038 761 1028 2301
Fragments arranged by size:
2301 2038 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2343 3450 4650 4735 5806 2343
Size: 1107 1200 85 1071 3126
Fragments arranged by size:
3126 1200 1107 1071 85
BsmI GAATG_Cn'
Cuts at: 2176 3557 4100 4193 2176
Size: 1381 543 93 4572
Fragments arranged by size:
4572 1381 543 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2343 2929 2980 3450 3908 4650 4735
Size: 399 553 586 51 470 458 742 85
Cuts at: 4735 5806 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 586 553 470 458 399
85 51
BspEI T'CCGG_A
Cuts at: 6252 6252
Size: 6589
BspHI T'CATG_A
Cuts at: 3421 3610 4478 6208 3421
Size: 189 868 1730 3802
Fragments arranged by size:
3802 1730 868 189
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2168 2280 3005 3156 3944 844
Size: 1324 112 725 151 788 3489
Fragments arranged by size:
3489 1324 788 725 151 112
BsrBI CCG'CTC
Cuts at: 1394 1823 2442 4476 1394
Size: 429 619 2034 3507
Fragments arranged by size:
3507 2034 619 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2251 2803 3259 5084 5258 65
Size: 1183 1003 552 456 1825 174 1396
Fragments arranged by size:
1825 1396 1183 1003 552 456 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2419 2943 5233 5420 1700
Size: 110 609 524 2290 187 2869
Fragments arranged by size:
2869 2290 609 524 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6589
BssHII G'CGCG_C
Cuts at: 1925 2016 1925
Size: 91 6498
BssSI C'ACGA_G
Cuts at: 4649 5661 4649
Size: 1012 5577
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2913 2997 794
Size: 2119 84 4386
Fragments arranged by size:
4386 2119 84
BstBI TT'CG_AA
Cuts at: 5393 5393
Size: 6589
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6589
BstXI CCAn_nnnn'nTGG
Cuts at: 2434 2453 3714 2434
Size: 19 1261 5309
Fragments arranged by size:
5309 1261 19
BstYI r'GATC_y
Cuts at: 1972 2209 2352 3919 4286 4671 4688 6140 6226
Size: 237 143 1567 367 385 17 1452 86
Cuts at: 6226 6238 6584 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1567 1452 385 367 346 237 143
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2283 2427 2872 2948 513
Size: 552 939 279 144 445 76 4154
Fragments arranged by size:
4154 939 552 445 279 144 76
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5850
Fragments arranged by size:
5850 683 56
BtsI GCAGTG_nn'
Cuts at: 2911 4105 4899 4919 2911
Size: 1194 794 20 4581
Fragments arranged by size:
4581 1194 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6589
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6554
DraI TTT'AAA
Cuts at: 3873 3893 3873
Size: 20 6569
DraIII CAC_nnn'GTG
Cuts at: 2319 2319
Size: 6589
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2703 5596 809
Size: 507 1387 2893 1802
Fragments arranged by size:
2893 1802 1387 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2052 2800 2945
Size: 54 1425 50 275 18 222 748 145
Cuts at: 2945 4036 4925 6554 8
Size: 1091 889 1629 43
Fragments arranged by size:
1629 1425 1091 889 748 275 222 145
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4036 1537
Size: 275 2224 4090
Fragments arranged by size:
4090 2224 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2727 4519 1353
Size: 1374 1792 3423
Fragments arranged by size:
3423 1792 1374
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5176 5550 5696 387
Size: 1064 138 3587 374 146 1280
Fragments arranged by size:
3587 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2097 2545 2601 2681 2818 3153 3695
Size: 393 465 448 56 80 137 335 542
Cuts at: 3695 4652 5249 6036 6304 1239
Size: 957 597 787 268 1524
Fragments arranged by size:
1524 957 787 597 542 465 448 393
335 268 137 80 56
EcoO109I rG'GnC_Cy
Cuts at: 2848 2848
Size: 6589
EcoRI G'AATT_C
Cuts at: 2375 3898 4276 2375
Size: 1523 378 4688
Fragments arranged by size:
4688 1523 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3151 3151
Size: 6589
FspI TGC'GCA
Cuts at: 3486 5095 3486
Size: 1609 4980
HaeII r_GCGC'y
Cuts at: 1678 2541 5736 1678
Size: 863 3195 2531
Fragments arranged by size:
3195 2531 863
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2337 2369 3198 3230 3375
Size: 32 135 32 765 32 829 32 145
Cuts at: 3375 3407 5230 5262 5304 5336 1373
Size: 32 1823 32 42 32 2626
Fragments arranged by size:
2626 1823 829 765 145 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3344 3933 4179 669
Size: 988 281 1406 589 246 3079
Fragments arranged by size:
3079 1406 988 589 281 246
HindIII A'AGCT_T
Cuts at: 748 2601 3820 748
Size: 1853 1219 3517
Fragments arranged by size:
3517 1853 1219
HpaI GTT'AAC
Cuts at: 4179 4179
Size: 6589
KpnI G_GTAC'C
Cuts at: 3914 3914
Size: 6589
MfeI C'AATT_G
Cuts at: 4188 4188
Size: 6589
MluI A'CGCG_T
Cuts at: 6465 6465
Size: 6589
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5703 5887 1305
Size: 4398 184 2007
Fragments arranged by size:
4398 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2802 10
Size: 54 1425 343 970 3797
Fragments arranged by size:
3797 1425 970 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2048 4547 4906 5065 518
Size: 648 882 2499 359 159 2042
Fragments arranged by size:
2499 2042 882 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2091 2659 2686 2787 2960 3212 4005 4682
Size: 485 568 27 101 173 252 793 677
Cuts at: 4682 5830 6075 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 793 677 568 485 252 245
173 101 27
NaeI GCC'GGC
Cuts at: 2421 2421
Size: 6589
NcoI C'CATG_G
Cuts at: 513 1065 2004 2283 2427 2872 2948 513
Size: 552 939 279 144 445 76 4154
Fragments arranged by size:
4154 939 552 445 279 144 76
NdeI CA'TA_TG
Cuts at: 387 3580 387
Size: 3193 3396
NgoMIV G'CCGG_C
Cuts at: 2419 2419
Size: 6589
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6589
NotI GC'GGCC_GC
Cuts at: 4036 4036
Size: 6589
NsiI A_TGCA'T
Cuts at: 2819 2819
Size: 6589
NspI r_CATG'y
Cuts at: 1132 1229 3017 3947 5492 1132
Size: 97 1788 930 1545 2229
Fragments arranged by size:
2229 1788 1545 930 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6589
PciI A'CATG_T
Cuts at: 5488 5488
Size: 6589
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2579 1108
Size: 1471 5118
PmeI GTTT'AAAC
Cuts at: 3893 3893
Size: 6589
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4658 4690 6171 6203 4658
Size: 32 1481 32 5044
Fragments arranged by size:
5044 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 3438 1613
Size: 1825 4764
PsiI TTA'TAA
Cuts at: 2475 4159 2475
Size: 1684 4905
PstI C_TGCA'G
Cuts at: 830 3941 830
Size: 3111 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6589
PvuII CAG'CTG
Cuts at: 2659 2686 2787 2960 3212 2659
Size: 27 101 173 252 6036
Fragments arranged by size:
6036 252 173 101 27
SalI G'TCGA_C
Cuts at: 1936 3342 3931 1936
Size: 1406 589 4594
Fragments arranged by size:
4594 1406 589
SapI GCTCTTCn'nnn_
Cuts at: 2727 2727
Size: 6589
SbfI CC_TGCA'GG
Cuts at: 3941 3941
Size: 6589
ScaI AGT'ACT
Cuts at: 2480 4837 6549 2480
Size: 2357 1712 2520
Fragments arranged by size:
2520 2357 1712
SfcI C'TryA_G
Cuts at: 826 1045 2194 3937 5072 5753 5944 826
Size: 219 1149 1743 1135 681 191 1471
Fragments arranged by size:
1743 1471 1149 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6589
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6589
SmaI CCC'GGG
Cuts at: 1872 3916 1872
Size: 2044 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4693 5594 5856 820
Size: 197 926 2750 901 262 1553
Fragments arranged by size:
2750 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4380 493
Size: 3887 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6589
SphI G_CATG'C
Cuts at: 1132 3017 3947 1132
Size: 1885 930 3774
Fragments arranged by size:
3774 1885 930
SspI AAT'ATT
Cuts at: 5 52 3575 4513 5
Size: 47 3523 938 2081
Fragments arranged by size:
3523 2081 938 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6589
StyI C'CwwG_G
Cuts at: 513 1065 2004 2283 2427 2872 2948 3552 4028
Size: 552 939 279 144 445 76 604 476
Cuts at: 4028 513
Size: 3074
Fragments arranged by size:
3074 939 604 552 476 445 279 144
76
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4616 4633 4786 4971 5416 325
Size: 1255 3036 17 153 185 445 1498
Fragments arranged by size:
3036 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2184 2478 2546
Size: 274 80 33 51 1443 207 294 68
Cuts at: 2546 4835 6547 96
Size: 2289 1712 138
Fragments arranged by size:
2289 1712 1443 294 274 207 138 80
68 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3052 3084 6549 6581 3052
Size: 32 3465 32 3060
Fragments arranged by size:
3465 3060 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6589
XbaI T'CTAG_A
Cuts at: 3925 3925
Size: 6589
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2870 3471 2870
Size: 601 5988
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6589
XmaI C'CCGG_G
Cuts at: 1870 3914 1870
Size: 2044 4545
XmnI GAAnn'nnTTC
Cuts at: 3680 4718 3680
Size: 1038 5551
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4397 276
Size: 53 83 186 936 2863 2468
Fragments arranged by size:
2863 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NsiI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI
SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BbeI BbvCI BplI Bpu10I
BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FseI
FspAI KasI NarI NruI PacI PfoI PmlI PpuMI
PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI
SfiI SfoI SrfI SwaI