Restriction map for
pFN21AB4576
(Circular) (Six-base) MAPSORT of: pFN21AB4576.seq Check: 6521 from: 1 to: 5272
pFN21AB4576 5272 bp
With 182 enzymes: SgfI *
April 15, 2010 09:37 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3082 278
Size: 53 83 186 936 1546 2468
Fragments arranged by size:
2468 1546 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2615 1937
Size: 678 4594
Acc65I G'GTAC_C
Cuts at: 2593 2593
Size: 5272
AclI AA'CG_TT
Cuts at: 1563 3399 3772 1563
Size: 1836 373 3063
Fragments arranged by size:
3063 1836 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2090 3335 4719 4987 1632
Size: 458 1245 1384 268 1917
Fragments arranged by size:
1917 1384 1245 458 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5075
AflIII A'CryG_T
Cuts at: 1203 4171 5148 1203
Size: 2968 977 1327
Fragments arranged by size:
2968 1327 977
AgeI A'CCGG_T
Cuts at: 4103 4103
Size: 5272
AhdI GACnn_n'nnGTC
Cuts at: 4001 4001
Size: 5272
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5272
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5272
AlwNI CAG_nnn'CTG
Cuts at: 2196 3015 4587 2196
Size: 819 1572 2881
Fragments arranged by size:
2881 1572 819
ApaLI G'TGCA_C
Cuts at: 3329 4485 3329
Size: 1156 4116
ApoI r'AATT_y
Cuts at: 1493 2581 2807 2959 5068 1493
Size: 1088 226 152 2109 1697
Fragments arranged by size:
2109 1697 1088 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3826 160
Size: 872 2794 1606
Fragments arranged by size:
2794 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5272
AvaI C'yCGr_G
Cuts at: 1870 1943 2597 1870
Size: 73 654 4545
Fragments arranged by size:
4545 654 73
BamHI G'GATC_C
Cuts at: 2484 2602 2969 2484
Size: 118 367 4787
Fragments arranged by size:
4787 367 118
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2327 2593 4048 618
Size: 325 217 627 540 266 1455 1842
Fragments arranged by size:
1842 1455 627 540 325 266 217
BanII G_rGCy'C
Cuts at: 1391 2190 2290 2591 1391
Size: 799 100 301 4072
Fragments arranged by size:
4072 799 301 100
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2078 2563 928
Size: 971 179 485 3637
Fragments arranged by size:
3637 971 485 179
BbvCI CC'TCA_GC
Cuts at: 2336 2336
Size: 5272
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2068 2102 3463 3497 1903
Size: 34 131 34 1361 34 3678
Fragments arranged by size:
3678 1361 131 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3164 4374 3164
Size: 1210 4062
BclI T'GATC_A
Cuts at: 1548 2349 1548
Size: 801 4471
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2185 2240
Size: 107 122 71 631 605 337 176 55
Cuts at: 2240 2322 3883 136
Size: 82 1561 1525
Fragments arranged by size:
1561 1525 631 605 337 176 122 107
82 71 55
BglII A'GATC_T
Cuts at: 5267 5267
Size: 5272
BlpI GC'TnA_GC
Cuts at: 2689 2689
Size: 5272
Bme1580I G_kGCm'C
Cuts at: 1790 2332 3333 4489 1790
Size: 542 1001 1156 2573
Fragments arranged by size:
2573 1156 1001 542
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5272
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3003 3961 442
Size: 416 53 83 399 24 1586 958 1753
Fragments arranged by size:
1753 1586 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2459 1056
Size: 1403 3869
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3932 1239
Size: 2693 2579
Bpu10I CC'TnA_GC
Cuts at: 2189 2336 2189
Size: 147 5125
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3397 4262 4560 3397
Size: 865 298 4109
Fragments arranged by size:
4109 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3935 882
Size: 617 2436 2219
Fragments arranged by size:
2436 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3063 493
Size: 711 1859 2702
Fragments arranged by size:
2702 1859 711
BsaBI GATnn'nnATC
Cuts at: 5266 5266
Size: 5272
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3079 3461 4096 275
Size: 53 83 186 936 1546 382 635 1451
Fragments arranged by size:
1546 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3705 4103 4377 4524 4935 3705
Size: 398 274 147 411 4042
Fragments arranged by size:
4042 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2277 2307 1178
Size: 30 165 30 874 30 4143
Fragments arranged by size:
4143 874 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2324 1186
Size: 1138 4134
BseYI C'CCAG_C
Cuts at: 4475 4971 4475
Size: 496 4776
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4699
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2554 2722 3483 4511 1540
Size: 275 186 553 168 761 1028 2301
Fragments arranged by size:
2301 1028 761 553 275 186 168
BsiHKAI G_wGCw'C
Cuts at: 2220 3333 3418 4489 2220
Size: 1113 85 1071 3003
Fragments arranged by size:
3003 1113 1071 85
BsmI GAATG_Cn'
Cuts at: 2783 2876 2783
Size: 93 5179
BsmBI CGTCTCn'nnnn_
Cuts at: 2212 2456 2212
Size: 244 5028
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2190 2220 2290 2332 2591 3333 3418
Size: 399 400 30 70 42 259 742 85
Cuts at: 3418 4489 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 400 399 259 85 70
42 30
BspEI T'CCGG_A
Cuts at: 4935 4935
Size: 5272
BspHI T'CATG_A
Cuts at: 3161 4891 3161
Size: 1730 3542
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2627 844
Size: 1783 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3159 1394
Size: 429 1336 3507
Fragments arranged by size:
3507 1336 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2158 2229 3767 3941 65
Size: 1183 910 71 1538 174 1396
Fragments arranged by size:
1538 1396 1183 910 174 71
BsrFI r'CCGG_y
Cuts at: 1700 1810 3916 4103 1700
Size: 110 2106 187 2869
Fragments arranged by size:
2869 2106 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5272
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5272
BssSI C'ACGA_G
Cuts at: 2057 3332 4344 2057
Size: 1275 1012 2985
Fragments arranged by size:
2985 1275 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5272
BstBI TT'CG_AA
Cuts at: 4076 4076
Size: 5272
BstEII G'GTnAC_C
Cuts at: 1184 2293 1184
Size: 1109 4163
BstYI r'GATC_y
Cuts at: 1972 2082 2484 2602 2969 3354 3371 4823 4909
Size: 110 402 118 367 385 17 1452 86
Cuts at: 4909 4921 5267 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 402 385 367 346 118 110
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3781
Fragments arranged by size:
3781 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2108 487
Size: 683 56 882 3651
Fragments arranged by size:
3651 882 683 56
BtsI GCAGTG_nn'
Cuts at: 2445 2788 3582 3602 2445
Size: 343 794 20 4115
Fragments arranged by size:
4115 794 343 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5272
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5237
DraI TTT'AAA
Cuts at: 2576 2576
Size: 5272
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4279 809
Size: 507 2963 1802
Fragments arranged by size:
2963 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2010 2551 2719
Size: 54 1425 50 275 18 180 541 168
Cuts at: 2719 3608 5237 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 541 275 180 168 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2551 2719 1537
Size: 275 739 168 4090
Fragments arranged by size:
4090 739 275 168
EarI CTCTTCn'nnn_
Cuts at: 1353 2225 3202 1353
Size: 872 977 3423
Fragments arranged by size:
3423 977 872
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2303 3859 4233 4379 387
Size: 1064 138 714 1556 374 146 1280
Fragments arranged by size:
1556 1280 1064 714 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2090 3335 3932 4719 4987 1239
Size: 393 458 1245 597 787 268 1524
Fragments arranged by size:
1524 1245 787 597 458 393 268
EcoRI G'AATT_C
Cuts at: 2581 2959 2581
Size: 378 4894
FspI TGC'GCA
Cuts at: 3778 3778
Size: 5272
HaeII r_GCGC'y
Cuts at: 1678 2246 4419 1678
Size: 568 2173 2531
Fragments arranged by size:
2531 2173 568
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3913 3945 3987 4019 1373
Size: 32 135 32 2341 32 42 32 2626
Fragments arranged by size:
2626 2341 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2616 2862 669
Size: 988 281 678 246 3079
Fragments arranged by size:
3079 988 678 281 246
HindIII A'AGCT_T
Cuts at: 748 2412 748
Size: 1664 3608
HpaI GTT'AAC
Cuts at: 2862 2862
Size: 5272
KpnI G_GTAC'C
Cuts at: 2597 2597
Size: 5272
MfeI C'AATT_G
Cuts at: 2871 2871
Size: 5272
MluI A'CGCG_T
Cuts at: 5148 5148
Size: 5272
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4386 4570 1305
Size: 3081 184 2007
Fragments arranged by size:
3081 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3450
Fragments arranged by size:
3450 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3230 3589 3748 518
Size: 648 2064 359 159 2042
Fragments arranged by size:
2064 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2688 3365 4513 4758 1606
Size: 1082 677 1148 245 2120
Fragments arranged by size:
2120 1148 1082 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3781
Fragments arranged by size:
3781 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5272
NheI G'CTAG_C
Cuts at: 1052 2455 1052
Size: 1403 3869
NotI GC'GGCC_GC
Cuts at: 2719 2719
Size: 5272
NspI r_CATG'y
Cuts at: 1132 1229 2630 4175 1132
Size: 97 1401 1545 2229
Fragments arranged by size:
2229 1545 1401 97
PasI CC'CwG_GG
Cuts at: 1348 2479 1348
Size: 1131 4141
PciI A'CATG_T
Cuts at: 4171 4171
Size: 5272
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2437 1108
Size: 1329 3943
PmeI GTTT'AAAC
Cuts at: 2576 2576
Size: 5272
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3341 3373 4854 4886 3341
Size: 32 1481 32 3727
Fragments arranged by size:
3727 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5272
PsiI TTA'TAA
Cuts at: 2842 2842
Size: 5272
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2351 2383 2351
Size: 32 5240
PstI C_TGCA'G
Cuts at: 830 2624 830
Size: 1794 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5272
SalI G'TCGA_C
Cuts at: 1936 2614 1936
Size: 678 4594
SapI GCTCTTCn'nnn_
Cuts at: 2225 2225
Size: 5272
SbfI CC_TGCA'GG
Cuts at: 2624 2624
Size: 5272
ScaI AGT'ACT
Cuts at: 3520 5232 3520
Size: 1712 3560
SfcI C'TryA_G
Cuts at: 826 1045 2620 3755 4436 4627 826
Size: 219 1575 1135 681 191 1471
Fragments arranged by size:
1575 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5272
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5272
SmaI CCC'GGG
Cuts at: 1872 2599 1872
Size: 727 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3376 4277 4539 820
Size: 197 926 1433 901 262 1553
Fragments arranged by size:
1553 1433 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3063 493
Size: 2570 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5272
SphI G_CATG'C
Cuts at: 1132 2630 1132
Size: 1498 3774
SspI AAT'ATT
Cuts at: 5 52 3196 5
Size: 47 3144 2081
Fragments arranged by size:
3144 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5272
StyI C'CwwG_G
Cuts at: 513 1065 2004 2711 513
Size: 552 939 707 3074
Fragments arranged by size:
3074 939 707 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3299 3316 3469 3654 4099 325
Size: 1255 1719 17 153 185 445 1498
Fragments arranged by size:
1719 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3518 5230 96
Size: 274 80 33 51 1443 1541 1712 138
Fragments arranged by size:
1712 1541 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2277 2309 5232 5264 2277
Size: 32 2923 32 2285
Fragments arranged by size:
2923 2285 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5272
XbaI T'CTAG_A
Cuts at: 2608 2608
Size: 5272
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5272
XmaI C'CCGG_G
Cuts at: 1870 2597 1870
Size: 727 4545
XmnI GAAnn'nnTTC
Cuts at: 3401 3401
Size: 5272
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3080 276
Size: 53 83 186 936 1546 2468
Fragments arranged by size:
2468 1546 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PsrI PstI PvuI SalI
SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BplI BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI
PspOMI PspXI PvuII RsrII SacI SacII SanDI SexAI
SfiI SfoI SrfI SwaI XcmI