Restriction map for
pFN21AB4575
(Circular) (Six-base) MAPSORT of: pFN21AB4575.seq Check: 8710 from: 1 to: 7360
pFN21AB4575 7360 bp
With 182 enzymes: SgfI *
October 20, 2009 10:34 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5170 278
Size: 53 83 186 936 3634 2468
Fragments arranged by size:
3634 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4703 1937
Size: 2766 4594
Acc65I G'GTAC_C
Cuts at: 3313 3928 4681 3313
Size: 615 753 5992
Fragments arranged by size:
5992 753 615
AclI AA'CG_TT
Cuts at: 1563 2431 3073 3230 5487 5860 1563
Size: 868 642 157 2257 373 3063
Fragments arranged by size:
3063 2257 868 642 373 157
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2144 2463 2737 3180 3347 3668 5423 6807
Size: 512 319 274 443 167 321 1755 1384
Cuts at: 6807 7075 1632
Size: 268 1917
Fragments arranged by size:
1917 1755 1384 512 443 321 319 274
268 167
AfeI AGC'GCT
Cuts at: 4574 4574
Size: 7360
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7163
AflIII A'CryG_T
Cuts at: 1203 2545 6259 7236 1203
Size: 1342 3714 977 1327
Fragments arranged by size:
3714 1342 1327 977
AgeI A'CCGG_T
Cuts at: 6191 6191
Size: 7360
AhdI GACnn_n'nnGTC
Cuts at: 6089 6089
Size: 7360
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7360
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3414 1184
Size: 2230 5130
AlwNI CAG_nnn'CTG
Cuts at: 3752 5103 6675 3752
Size: 1351 1572 4437
Fragments arranged by size:
4437 1572 1351
ApaLI G'TGCA_C
Cuts at: 3899 5417 6573 3899
Size: 1518 1156 4686
Fragments arranged by size:
4686 1518 1156
ApoI r'AATT_y
Cuts at: 1493 2910 3112 3255 3463 4669 4895 5047 7156
Size: 1417 202 143 208 1206 226 152 2109
Cuts at: 7156 1493
Size: 1697
Fragments arranged by size:
2109 1697 1417 1206 226 208 202 152
143
AseI AT'TA_AT
Cuts at: 160 1032 5914 160
Size: 872 4882 1606
Fragments arranged by size:
4882 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7360
AvaI C'yCGr_G
Cuts at: 1870 1943 2176 4685 1870
Size: 73 233 2509 4545
Fragments arranged by size:
4545 2509 233 73
BamHI G'GATC_C
Cuts at: 4690 5057 4690
Size: 367 6993
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3313 3928 4681 6136 618
Size: 325 217 627 1526 615 753 1455 1842
Fragments arranged by size:
1842 1526 1455 753 627 615 325 217
BanII G_rGCy'C
Cuts at: 1391 2333 3599 4679 1391
Size: 942 1266 1080 4072
Fragments arranged by size:
4072 1266 1080 942
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 4548 928
Size: 971 2649 3740
Fragments arranged by size:
3740 2649 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5551 5585 1903
Size: 34 3614 34 3678
Fragments arranged by size:
3678 3614 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5252 6462 5252
Size: 1210 6150
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7360
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5971 136
Size: 107 122 71 631 605 4299 1525
Fragments arranged by size:
4299 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7355 7355
Size: 7360
BlpI GC'TnA_GC
Cuts at: 4569 4777 4569
Size: 208 7152
Bme1580I G_kGCm'C
Cuts at: 1790 2494 2990 3903 5421 6577 1790
Size: 704 496 913 1518 1156 2573
Fragments arranged by size:
2573 1518 1156 913 704 496
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7360
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5091 6049 442
Size: 416 53 83 399 24 3674 958 1753
Fragments arranged by size:
3674 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7360
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3594 3594
Size: 7360
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2922 3407 3879 4548 6020 1239
Size: 1683 485 472 669 1472 2579
Fragments arranged by size:
2579 1683 1472 669 485 472
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2317 4597 5485 6350 6648 2317
Size: 2280 888 865 298 3029
Fragments arranged by size:
3029 2280 888 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6023 882
Size: 617 4524 2219
Fragments arranged by size:
4524 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 5151 493
Size: 711 3947 2702
Fragments arranged by size:
3947 2702 711
BsaBI GATnn'nnATC
Cuts at: 2614 4269 7354 2614
Size: 1655 3085 2620
Fragments arranged by size:
3085 2620 1655
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4171 5167 5549 6184
Size: 53 83 186 936 2638 996 382 635
Cuts at: 6184 275
Size: 1451
Fragments arranged by size:
2638 1451 996 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5793 6191 6465 6612 7023 5793
Size: 398 274 147 411 6130
Fragments arranged by size:
6130 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3791 3821 1178
Size: 30 165 30 2388 30 4717
Fragments arranged by size:
4717 2388 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2164 2341 2732 4483 4529 1186
Size: 978 177 391 1751 46 4017
Fragments arranged by size:
4017 1751 978 391 177 46
BseYI C'CCAG_C
Cuts at: 2797 6563 7059 2797
Size: 3766 496 3098
Fragments arranged by size:
3766 3098 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3589 3771 1163
Size: 573 1853 182 4752
Fragments arranged by size:
4752 1853 573 182
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4810 5571 6599 1540
Size: 275 186 2809 761 1028 2301
Fragments arranged by size:
2809 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2333 2788 3580 3599 3903 3969 4012 5421 5506
Size: 455 792 19 304 66 43 1409 85
Cuts at: 5506 6577 2333
Size: 1071 3116
Fragments arranged by size:
3116 1409 1071 792 455 304 85 66
43 19
BsmI GAATG_Cn'
Cuts at: 3403 4871 4964 3403
Size: 1468 93 5799
Fragments arranged by size:
5799 1468 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2333 2494 2788 2990 3580 3599 3903
Size: 399 543 161 294 202 590 19 304
Cuts at: 3903 3969 4012 4679 5421 5506 6577 1391
Size: 66 43 667 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 667 590 543 399 304
294 202 161 85 66 43 19
BspEI T'CCGG_A
Cuts at: 7023 7023
Size: 7360
BspHI T'CATG_A
Cuts at: 3778 4174 4390 5249 6979 3778
Size: 396 216 859 1730 4159
Fragments arranged by size:
4159 1730 859 396 216
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4715 844
Size: 3871 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 5247 1394
Size: 429 3424 3507
Fragments arranged by size:
3507 3424 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3373 5855 6029 65
Size: 1183 2125 2482 174 1396
Fragments arranged by size:
2482 2125 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6004 6191 1700
Size: 110 4194 187 2869
Fragments arranged by size:
4194 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7360
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7360
BssSI C'ACGA_G
Cuts at: 5420 6432 5420
Size: 1012 6348
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7360
BstBI TT'CG_AA
Cuts at: 6164 6164
Size: 7360
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7360
BstXI CCAn_nnnn'nTGG
Cuts at: 2032 2032
Size: 7360
BstYI r'GATC_y
Cuts at: 1972 4690 5057 5442 5459 6911 6997 7009 7355
Size: 2718 367 385 17 1452 86 12 346
Cuts at: 7355 1972
Size: 1977
Fragments arranged by size:
2718 1977 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 6808
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4521 487
Size: 683 56 3295 3326
Fragments arranged by size:
3326 3295 683 56
BtsI GCAGTG_nn'
Cuts at: 3709 4417 4876 5670 5690 3709
Size: 708 459 794 20 5379
Fragments arranged by size:
5379 794 708 459 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7360
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7325
DraI TTT'AAA
Cuts at: 3001 3078 4664 3001
Size: 77 1586 5697
Fragments arranged by size:
5697 1586 77
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2756 6367 809
Size: 507 1440 3611 1802
Fragments arranged by size:
3611 1802 1440 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4807 5696 7325
Size: 54 1425 50 275 18 2977 889 1629
Cuts at: 7325 8
Size: 43
Fragments arranged by size:
2977 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4807 1537
Size: 275 2995 4090
Fragments arranged by size:
4090 2995 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4088 4167 4228 4501 4506 4563 4624 5290
Size: 2735 79 61 273 5 57 61 666
Cuts at: 5290 1353
Size: 3423
Fragments arranged by size:
3423 2735 666 273 79 61 61 57
5
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2212 5947 6321 6467 387
Size: 1064 138 623 3735 374 146 1280
Fragments arranged by size:
3735 1280 1064 623 374 146 138
EcoICRI GAG'CTC
Cuts at: 2331 3597 2331
Size: 1266 6094
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2144 2463 2737 2922 3180 3347 3407
Size: 393 512 319 274 185 258 167 60
Cuts at: 3407 3668 3879 4548 5423 6020 6807 7075 1239
Size: 261 211 669 875 597 787 268 1524
Fragments arranged by size:
1524 875 787 669 597 512 393 319
274 268 261 258 211 185 167 60
EcoNI CCTnn'n_nnAGG
Cuts at: 2394 3570 2394
Size: 1176 6184
EcoO109I rG'GnC_Cy
Cuts at: 2801 3944 2801
Size: 1143 6217
EcoRI G'AATT_C
Cuts at: 3255 4669 5047 3255
Size: 1414 378 5568
Fragments arranged by size:
5568 1414 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3875 3875
Size: 7360
FspI TGC'GCA
Cuts at: 5866 5866
Size: 7360
HaeII r_GCGC'y
Cuts at: 1678 2316 4576 6507 1678
Size: 638 2260 1931 2531
Fragments arranged by size:
2531 2260 1931 638
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2138 2170 3110 3142 4475
Size: 32 135 32 566 32 940 32 1333
Cuts at: 4475 4478 4507 4510 6001 6033 6075 6107 1373
Size: 3 29 3 1491 32 42 32 2626
Fragments arranged by size:
2626 1491 1333 940 566 135 42 32
32 32 32 32 32 29 3 3
HincII GTy'rAC
Cuts at: 669 1657 1938 2853 3234 4704 4950 669
Size: 988 281 915 381 1470 246 3079
Fragments arranged by size:
3079 1470 988 915 381 281 246
HindIII A'AGCT_T
Cuts at: 748 3668 4286 748
Size: 2920 618 3822
Fragments arranged by size:
3822 2920 618
HpaI GTT'AAC
Cuts at: 3234 4950 3234
Size: 1716 5644
KpnI G_GTAC'C
Cuts at: 3317 3932 4685 3317
Size: 615 753 5992
Fragments arranged by size:
5992 753 615
MfeI C'AATT_G
Cuts at: 4959 4959
Size: 7360
MluI A'CGCG_T
Cuts at: 7236 7236
Size: 7360
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3294 4570 6474 6658 1305
Size: 1989 1276 1904 184 2007
Fragments arranged by size:
2007 1989 1904 1276 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5538
Fragments arranged by size:
5538 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2036 3816 3827 4395 5318 5677 5836
Size: 648 870 1780 11 568 923 359 159
Cuts at: 5836 518
Size: 2042
Fragments arranged by size:
2042 1780 923 870 648 568 359 159
11
MspA1I CmG'CkG
Cuts at: 1606 3522 3739 4776 5453 6601 6846 1606
Size: 1916 217 1037 677 1148 245 2120
Fragments arranged by size:
2120 1916 1148 1037 677 245 217
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6808
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7360
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7360
NotI GC'GGCC_GC
Cuts at: 4807 4807
Size: 7360
NspI r_CATG'y
Cuts at: 1132 1229 2549 2728 2874 3832 4718 6263 1132
Size: 97 1320 179 146 958 886 1545 2229
Fragments arranged by size:
2229 1545 1320 958 886 179 146 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7360
PciI A'CATG_T
Cuts at: 2545 6259 2545
Size: 3714 3646
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2010 2213 1108
Size: 902 203 6255
Fragments arranged by size:
6255 902 203
PfoI T'CCnGG_A
Cuts at: 3910 3910
Size: 7360
PmeI GTTT'AAAC
Cuts at: 4664 4664
Size: 7360
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5429 5461 6942 6974 5429
Size: 32 1481 32 5815
Fragments arranged by size:
5815 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2801 3944 2801
Size: 1143 6217
PshAI GACnn'nnGTC
Cuts at: 1613 4458 1613
Size: 2845 4515
PsiI TTA'TAA
Cuts at: 2816 4930 2816
Size: 2114 5246
PstI C_TGCA'G
Cuts at: 830 3744 3762 4412 4712 830
Size: 2914 18 650 300 3478
Fragments arranged by size:
3478 2914 650 300 18
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7360
PvuII CAG'CTG
Cuts at: 3739 3739
Size: 7360
SacI G_AGCT'C
Cuts at: 2333 3599 2333
Size: 1266 6094
SalI G'TCGA_C
Cuts at: 1936 4702 1936
Size: 2766 4594
SanDI GG'GwC_CC
Cuts at: 3944 3944
Size: 7360
SbfI CC_TGCA'GG
Cuts at: 4712 4712
Size: 7360
ScaI AGT'ACT
Cuts at: 3432 5608 7320 3432
Size: 2176 1712 3472
Fragments arranged by size:
3472 2176 1712
SfcI C'TryA_G
Cuts at: 826 1045 3740 3758 4408 4456 4602 4708 5843
Size: 219 2695 18 650 48 146 106 1135
Cuts at: 5843 6524 6715 826
Size: 681 191 1471
Fragments arranged by size:
2695 1471 1135 681 650 219 191 146
106 48 18
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7360
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7360
SmaI CCC'GGG
Cuts at: 1872 4687 1872
Size: 2815 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2332 4576 5464 6365 6627 820
Size: 197 926 389 2244 888 901 262 1553
Fragments arranged by size:
2244 1553 926 901 888 389 262 197
SnaBI TAC'GTA
Cuts at: 493 5151 493
Size: 4658 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7360
SphI G_CATG'C
Cuts at: 1132 4718 1132
Size: 3586 3774
SspI AAT'ATT
Cuts at: 5 52 2595 5284 5
Size: 47 2543 2689 2081
Fragments arranged by size:
2689 2543 2081 47
StuI AGG'CCT
Cuts at: 1517 2389 3656 1517
Size: 872 1267 5221
Fragments arranged by size:
5221 1267 872
StyI C'CwwG_G
Cuts at: 513 1065 2226 4799 513
Size: 552 1161 2573 3074
Fragments arranged by size:
3074 2573 1161 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5387 5404 5557 5742 6187 325
Size: 1255 3807 17 153 185 445 1498
Fragments arranged by size:
3807 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2763 3430 4478
Size: 274 80 33 51 1443 786 667 1048
Cuts at: 4478 5606 7318 96
Size: 1128 1712 138
Fragments arranged by size:
1712 1443 1128 1048 786 667 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2767 2799 3791 3823 3846 3878 4110 4142 7320
Size: 32 992 32 23 32 232 32 3178
Cuts at: 7320 7352 2767
Size: 32 2775
Fragments arranged by size:
3178 2775 992 232 32 32 32 32
32 23
Tth111I GACn'n_nGTC
Cuts at: 1316 2193 1316
Size: 877 6483
XbaI T'CTAG_A
Cuts at: 4696 4696
Size: 7360
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2032 4208 2011
Size: 21 2176 5163
Fragments arranged by size:
5163 2176 21
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7360
XmaI C'CCGG_G
Cuts at: 1870 4685 1870
Size: 2815 4545
XmnI GAAnn'nnTTC
Cuts at: 2462 2534 3193 5489 2462
Size: 72 659 2296 4333
Fragments arranged by size:
4333 2296 659 72
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5168 276
Size: 53 83 186 936 3634 2468
Fragments arranged by size:
3634 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI PvuII SacI SalI SanDI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PmlI PspOMI PspXI PsrI RsrII SacII SapI
SexAI SfiI SfoI SrfI SwaI