Restriction map for
pFN21AB4569
(Circular) (Six-base) MAPSORT of: pFN21AB4569.seq Check: 5457 from: 1 to: 6349
pFN21AB4569 6349 bp
With 182 enzymes: SgfI *
October 20, 2009 10:59 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2356 3401 3605 2356
Size: 1045 204 5100
Fragments arranged by size:
5100 1045 204
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4159 278
Size: 53 83 186 936 2623 2468
Fragments arranged by size:
2623 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3357 3692 1937
Size: 1420 335 4594
Fragments arranged by size:
4594 1420 335
Acc65I G'GTAC_C
Cuts at: 3670 3670
Size: 6349
AclI AA'CG_TT
Cuts at: 1563 4476 4849 1563
Size: 2913 373 3063
Fragments arranged by size:
3063 2913 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2079 2483 4412 5796 6064 1632
Size: 447 404 1929 1384 268 1917
Fragments arranged by size:
1929 1917 1384 447 404 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6152
AflIII A'CryG_T
Cuts at: 1203 5248 6225 1203
Size: 4045 977 1327
Fragments arranged by size:
4045 1327 977
AgeI A'CCGG_T
Cuts at: 5180 5180
Size: 6349
AhdI GACnn_n'nnGTC
Cuts at: 5078 5078
Size: 6349
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6349
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3266 1184
Size: 2082 4267
AlwNI CAG_nnn'CTG
Cuts at: 3492 4092 5664 3492
Size: 600 1572 4177
Fragments arranged by size:
4177 1572 600
ApaI G_GGCC'C
Cuts at: 3379 3379
Size: 6349
ApaLI G'TGCA_C
Cuts at: 4406 5562 4406
Size: 1156 5193
ApoI r'AATT_y
Cuts at: 1493 2048 2246 3658 3884 4036 6145 1493
Size: 555 198 1412 226 152 2109 1697
Fragments arranged by size:
2109 1697 1412 555 226 198 152
AseI AT'TA_AT
Cuts at: 160 1032 4903 160
Size: 872 3871 1606
Fragments arranged by size:
3871 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6349
AvaI C'yCGr_G
Cuts at: 1870 1943 3674 1870
Size: 73 1731 4545
Fragments arranged by size:
4545 1731 73
BamHI G'GATC_C
Cuts at: 2529 3679 4046 2529
Size: 1150 367 4832
Fragments arranged by size:
4832 1150 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2326 3389 3670 5125 618
Size: 325 217 627 539 1063 281 1455 1842
Fragments arranged by size:
1842 1455 1063 627 539 325 281 217
BanII G_rGCy'C
Cuts at: 1391 2866 3379 3668 1391
Size: 1475 513 289 4072
Fragments arranged by size:
4072 1475 513 289
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2910 3212 928
Size: 971 1011 302 4065
Fragments arranged by size:
4065 1011 971 302
BbvCI CC'TCA_GC
Cuts at: 2315 2315
Size: 6349
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4540 4574 1903
Size: 34 2603 34 3678
Fragments arranged by size:
3678 2603 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4241 5451 4241
Size: 1210 5139
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6349
BfrBI ATG'CAT
Cuts at: 2987 2987
Size: 6349
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2553 4960 136
Size: 107 122 71 631 605 881 2407 1525
Fragments arranged by size:
2407 1525 881 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6344 6344
Size: 6349
BlpI GC'TnA_GC
Cuts at: 3766 3766
Size: 6349
Bme1580I G_kGCm'C
Cuts at: 1790 2175 3379 3392 4410 5566 1790
Size: 385 1204 13 1018 1156 2573
Fragments arranged by size:
2573 1204 1156 1018 385 13
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6349
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2572 4080 5038
Size: 416 53 83 399 24 1155 1508 958
Cuts at: 5038 442
Size: 1753
Fragments arranged by size:
1753 1508 1155 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6349
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2878 3040 3339 5009 1239
Size: 1639 162 299 1670 2579
Fragments arranged by size:
2579 1670 1639 299 162
Bpu10I CC'TnA_GC
Cuts at: 2027 2315 2027
Size: 288 6061
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4474 5339 5637 4474
Size: 865 298 5186
Fragments arranged by size:
5186 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3530 5012 882
Size: 617 2031 1482 2219
Fragments arranged by size:
2219 2031 1482 617
BsaAI yAC'GTr
Cuts at: 493 1204 2131 4140 493
Size: 711 927 2009 2702
Fragments arranged by size:
2702 2009 927 711
BsaBI GATnn'nnATC
Cuts at: 2309 6343 2309
Size: 4034 2315
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4156 4538 5173 275
Size: 53 83 186 936 2623 382 635 1451
Fragments arranged by size:
2623 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4782 5180 5454 5601 6012 4782
Size: 398 274 147 411 5119
Fragments arranged by size:
5119 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6124
Fragments arranged by size:
6124 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2024 2583 2738 2741 3128 1186
Size: 838 559 155 3 387 4407
Fragments arranged by size:
4407 838 559 387 155 3
BseYI C'CCAG_C
Cuts at: 2702 2875 5552 6048 2702
Size: 173 2677 496 3003
Fragments arranged by size:
3003 2677 496 173
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3633 1163
Size: 573 1897 3879
Fragments arranged by size:
3879 1897 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3598 3799 4560 5588 1540
Size: 275 186 1597 201 761 1028 2301
Fragments arranged by size:
2301 1597 1028 761 275 201 186
BsiHKAI G_wGCw'C
Cuts at: 2541 2866 3282 3421 4410 4495 5566 2541
Size: 325 416 139 989 85 1071 3324
Fragments arranged by size:
3324 1071 989 416 325 139 85
BsmI GAATG_Cn'
Cuts at: 2987 3609 3860 3953 2987
Size: 622 251 93 5383
Fragments arranged by size:
5383 622 251 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2175 2541 2866 3282 3379 3392 3421
Size: 399 385 366 325 416 97 13 29
Cuts at: 3421 3668 4410 4495 5566 1391
Size: 247 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 416 399 385 366 325
247 97 85 29 13
BspEI T'CCGG_A
Cuts at: 6012 6012
Size: 6349
BspHI T'CATG_A
Cuts at: 4238 5968 4238
Size: 1730 4619
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2356 3401 3605 3704 844
Size: 1512 1045 204 99 3489
Fragments arranged by size:
3489 1512 1045 204 99
BsrBI CCG'CTC
Cuts at: 1394 1823 2746 4236 1394
Size: 429 923 1490 3507
Fragments arranged by size:
3507 1490 923 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2338 2372 3297 4844 5018 65
Size: 1183 1090 34 925 1547 174 1396
Fragments arranged by size:
1547 1396 1183 1090 925 174 34
BsrFI r'CCGG_y
Cuts at: 1700 1810 2038 4993 5180 1700
Size: 110 228 2955 187 2869
Fragments arranged by size:
2955 2869 228 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6349
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6349
BssSI C'ACGA_G
Cuts at: 4409 5421 4409
Size: 1012 5337
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6349
BstBI TT'CG_AA
Cuts at: 5153 5153
Size: 6349
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6349
BstXI CCAn_nnnn'nTGG
Cuts at: 2875 2875
Size: 6349
BstYI r'GATC_y
Cuts at: 1972 2529 3679 4046 4431 4448 5900 5986 5998
Size: 557 1150 367 385 17 1452 86 12
Cuts at: 5998 6344 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1150 557 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 3351 3351
Size: 6349
BtgI C'CryG_G
Cuts at: 513 1065 2004 2298 3210 3363 513
Size: 552 939 294 912 153 3499
Fragments arranged by size:
3499 939 912 552 294 153
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3421 487
Size: 683 56 2195 3415
Fragments arranged by size:
3415 2195 683 56
BtsI GCAGTG_nn'
Cuts at: 2229 3865 4659 4679 2229
Size: 1636 794 20 3899
Fragments arranged by size:
3899 1636 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6349
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6314
DraI TTT'AAA
Cuts at: 3653 3653
Size: 6349
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5356 809
Size: 507 4040 1802
Fragments arranged by size:
4040 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3595 3796 4685
Size: 54 1425 50 275 18 1765 201 889
Cuts at: 4685 6314 8
Size: 1629 43
Fragments arranged by size:
1765 1629 1425 889 275 201 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3595 3796 1537
Size: 275 1783 201 4090
Fragments arranged by size:
4090 1783 275 201
EarI CTCTTCn'nnn_
Cuts at: 1353 3032 3299 4279 1353
Size: 1679 267 980 3423
Fragments arranged by size:
3423 1679 980 267
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4936 5310 5456 387
Size: 1064 138 3347 374 146 1280
Fragments arranged by size:
3347 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2864 2864
Size: 6349
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2079 2483 2878 3040 3339 4412 5009
Size: 393 447 404 395 162 299 1073 597
Cuts at: 5009 5796 6064 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1073 787 597 447 404 395 393
299 268 162
EcoNI CCTnn'n_nnAGG
Cuts at: 2839 3110 2839
Size: 271 6078
EcoO109I rG'GnC_Cy
Cuts at: 2545 2923 3376 2545
Size: 378 453 5518
Fragments arranged by size:
5518 453 378
EcoRI G'AATT_C
Cuts at: 3658 4036 3658
Size: 378 5971
FspI TGC'GCA
Cuts at: 4855 4855
Size: 6349
HaeII r_GCGC'y
Cuts at: 1678 5496 1678
Size: 3818 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2789 2821 4990 5022 5064
Size: 32 135 32 1217 32 2169 32 42
Cuts at: 5064 5096 1373
Size: 32 2626
Fragments arranged by size:
2626 2169 1217 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3693 3939 669
Size: 988 281 1755 246 3079
Fragments arranged by size:
3079 1755 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6349
HpaI GTT'AAC
Cuts at: 3939 3939
Size: 6349
KpnI G_GTAC'C
Cuts at: 3674 3674
Size: 6349
MfeI C'AATT_G
Cuts at: 3948 3948
Size: 6349
MluI A'CGCG_T
Cuts at: 6225 6225
Size: 6349
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3041 3247 5463 5647 1305
Size: 1736 206 2216 184 2007
Fragments arranged by size:
2216 2007 1736 206 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4527
Fragments arranged by size:
4527 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2303 4307 4666 4825 518
Size: 648 1137 2004 359 159 2042
Fragments arranged by size:
2042 2004 1137 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2035 2168 2335 2738 2875 3399 3765 4442
Size: 429 133 167 403 137 524 366 677
Cuts at: 4442 5590 5835 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 677 524 429 403 366 245
167 137 133
NcoI C'CATG_G
Cuts at: 513 1065 2004 2298 3210 513
Size: 552 939 294 912 3652
Fragments arranged by size:
3652 939 912 552 294
NdeI CA'TA_TG
Cuts at: 387 3337 387
Size: 2950 3399
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6349
NotI GC'GGCC_GC
Cuts at: 3796 3796
Size: 6349
NsiI A_TGCA'T
Cuts at: 2989 2989
Size: 6349
NspI r_CATG'y
Cuts at: 1132 1229 3707 5252 1132
Size: 97 2478 1545 2229
Fragments arranged by size:
2478 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6349
PciI A'CATG_T
Cuts at: 5248 5248
Size: 6349
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2421 3589 1108
Size: 1313 1168 3868
Fragments arranged by size:
3868 1313 1168
PmeI GTTT'AAAC
Cuts at: 3653 3653
Size: 6349
PmlI CAC'GTG
Cuts at: 2131 2131
Size: 6349
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3623 3655 4418 4450 5931 5963 3623
Size: 32 763 32 1481 32 4009
Fragments arranged by size:
4009 1481 763 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2923 2923
Size: 6349
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6349
PsiI TTA'TAA
Cuts at: 3919 3919
Size: 6349
PspOMI G'GGCC_C
Cuts at: 3375 3375
Size: 6349
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2585 2617 2585
Size: 32 6317
PstI C_TGCA'G
Cuts at: 830 3245 3398 3558 3701 830
Size: 2415 153 160 143 3478
Fragments arranged by size:
3478 2415 160 153 143
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6349
PvuII CAG'CTG
Cuts at: 2035 2168 2335 2738 2035
Size: 133 167 403 5646
Fragments arranged by size:
5646 403 167 133
SacI G_AGCT'C
Cuts at: 2866 2866
Size: 6349
SalI G'TCGA_C
Cuts at: 1936 3691 1936
Size: 1755 4594
SbfI CC_TGCA'GG
Cuts at: 3701 3701
Size: 6349
ScaI AGT'ACT
Cuts at: 4597 6309 4597
Size: 1712 4637
SexAI A'CCwGG_T
Cuts at: 3623 3623
Size: 6349
SfcI C'TryA_G
Cuts at: 826 1045 3072 3241 3394 3554 3697 4832 5513
Size: 219 2027 169 153 160 143 1135 681
Cuts at: 5513 5704 826
Size: 191 1471
Fragments arranged by size:
2027 1471 1135 681 219 191 169 160
153 143
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6349
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6349
SmaI CCC'GGG
Cuts at: 1872 3676 1872
Size: 1804 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4453 5354 5616 820
Size: 197 926 2510 901 262 1553
Fragments arranged by size:
2510 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4140 493
Size: 3647 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6349
SphI G_CATG'C
Cuts at: 1132 3707 1132
Size: 2575 3774
SspI AAT'ATT
Cuts at: 5 52 4273 5
Size: 47 4221 2081
Fragments arranged by size:
4221 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6349
StyI C'CwwG_G
Cuts at: 513 1065 2004 2175 2298 3210 3788 513
Size: 552 939 171 123 912 578 3074
Fragments arranged by size:
3074 939 912 578 552 171 123
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2922 3404 4376 4393 4546 4731 5176
Size: 1255 1342 482 972 17 153 185 445
Cuts at: 5176 325
Size: 1498
Fragments arranged by size:
1498 1342 1255 972 482 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2487 4595 6307
Size: 274 80 33 51 1443 510 2108 1712
Cuts at: 6307 96
Size: 138
Fragments arranged by size:
2108 1712 1443 510 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3119 3151 3261 3293 6309 6341 3119
Size: 32 110 32 3016 32 3127
Fragments arranged by size:
3127 3016 110 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6349
XbaI T'CTAG_A
Cuts at: 3685 3685
Size: 6349
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6349
XmaI C'CCGG_G
Cuts at: 1870 3674 1870
Size: 1804 4545
XmnI GAAnn'nnTTC
Cuts at: 4478 4478
Size: 6349
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4157 276
Size: 53 83 186 936 2623 2468
Fragments arranged by size:
2623 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NsiI NspI PasI PciI PflMI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PsrI
PstI PvuI PvuII SacI SalI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BplI BsiWI
BsmBI BstZ17I DraIII EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI PacI PfoI PspXI RsrII
SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI