Restriction map for
pFN21AB4563
(Circular) (Six-base) MAPSORT of: pFN21AB4563.seq Check: 8062 from: 1 to: 7435
pFN21AB4563 7435 bp
With 182 enzymes: SgfI *
October 20, 2009 10:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4357 4357
Size: 7435
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5245 278
Size: 53 83 186 936 3709 2468
Fragments arranged by size:
3709 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2537 4778 1937
Size: 600 2241 4594
Fragments arranged by size:
4594 2241 600
Acc65I G'GTAC_C
Cuts at: 2828 4239 4337 4756 2828
Size: 1411 98 419 5507
Fragments arranged by size:
5507 1411 419 98
AclI AA'CG_TT
Cuts at: 1563 5562 5935 1563
Size: 3999 373 3063
Fragments arranged by size:
3999 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2759 2891 3551 3592 3606 3718 4339 5498
Size: 1127 132 660 41 14 112 621 1159
Cuts at: 5498 6882 7150 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1384 1159 1127 660 621 268 132
112 41 14
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7238
AflIII A'CryG_T
Cuts at: 1203 3198 4282 6334 7311 1203
Size: 1995 1084 2052 977 1327
Fragments arranged by size:
2052 1995 1327 1084 977
AgeI A'CCGG_T
Cuts at: 6266 6266
Size: 7435
AhdI GACnn_n'nnGTC
Cuts at: 2048 6164 2048
Size: 4116 3319
AleI CACnn'nnGTG
Cuts at: 1166 4364 4503 1166
Size: 3198 139 4098
Fragments arranged by size:
4098 3198 139
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3051 3497 4382 1184
Size: 1867 446 885 4237
Fragments arranged by size:
4237 1867 885 446
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4598 4630 4598
Size: 32 7403
AlwNI CAG_nnn'CTG
Cuts at: 3055 3302 3556 4483 5178 6750 3055
Size: 247 254 927 695 1572 3740
Fragments arranged by size:
3740 1572 927 695 254 247
ApaI G_GGCC'C
Cuts at: 2635 3982 2635
Size: 1347 6088
ApaLI G'TGCA_C
Cuts at: 5492 6648 5492
Size: 1156 6279
ApoI r'AATT_y
Cuts at: 1493 2750 3215 4220 4718 4744 4970 5122 7231
Size: 1257 465 1005 498 26 226 152 2109
Cuts at: 7231 1493
Size: 1697
Fragments arranged by size:
2109 1697 1257 1005 498 465 226 152
26
AseI AT'TA_AT
Cuts at: 160 1032 5989 160
Size: 872 4957 1606
Fragments arranged by size:
4957 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7435
AvaI C'yCGr_G
Cuts at: 1870 1943 2250 2919 3210 4248 4362 4760 1870
Size: 73 307 669 291 1038 114 398 4545
Fragments arranged by size:
4545 1038 669 398 307 291 114 73
BamHI G'GATC_C
Cuts at: 4765 5132 4765
Size: 367 7068
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2828 3848 4115 4239 4337
Size: 325 217 627 1041 1020 267 124 98
Cuts at: 4337 4411 4756 6211 618
Size: 74 345 1455 1842
Fragments arranged by size:
1842 1455 1041 1020 627 345 325 267
217 124 98 74
BanII G_rGCy'C
Cuts at: 1391 2235 2635 2865 3697 3732 3982 4184 4754
Size: 844 400 230 832 35 250 202 570
Cuts at: 4754 1391
Size: 4072
Fragments arranged by size:
4072 844 832 570 400 250 230 202
35
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3598 4279 928
Size: 971 1699 681 4084
Fragments arranged by size:
4084 1699 971 681
BbvCI CC'TCA_GC
Cuts at: 2156 2936 3357 2156
Size: 780 421 6234
Fragments arranged by size:
6234 780 421
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3808 3842 4253 4287 5626 5660 1903
Size: 34 1871 34 411 34 1339 34 3678
Fragments arranged by size:
3678 1871 1339 411 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2212 3526 5327 6537 2212
Size: 1314 1801 1210 3110
Fragments arranged by size:
3110 1801 1314 1210
BclI T'GATC_A
Cuts at: 1548 3390 4626 1548
Size: 1842 1236 4357
Fragments arranged by size:
4357 1842 1236
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3947 6046 136
Size: 107 122 71 631 605 2275 2099 1525
Fragments arranged by size:
2275 2099 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7430 7430
Size: 7435
BlpI GC'TnA_GC
Cuts at: 2398 4852 2398
Size: 2454 4981
Bme1580I G_kGCm'C
Cuts at: 1790 2635 2690 3982 4249 4416 5496 6652 1790
Size: 845 55 1292 267 167 1080 1156 2573
Fragments arranged by size:
2573 1292 1156 1080 845 267 167 55
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7435
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4114 5166 6124
Size: 416 53 83 399 24 2697 1052 958
Cuts at: 6124 442
Size: 1753
Fragments arranged by size:
2697 1753 1052 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 4047 1056
Size: 2991 4444
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2709 2819 3158 4043 6095 1239
Size: 1470 110 339 885 2052 2579
Fragments arranged by size:
2579 2052 1470 885 339 110
Bpu10I CC'TnA_GC
Cuts at: 2139 2156 2436 2936 3357 2139
Size: 17 280 500 421 6217
Fragments arranged by size:
6217 500 421 280 17
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2516 4362 5560 6425 6723 2516
Size: 1846 1198 865 298 3228
Fragments arranged by size:
3228 1846 1198 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4103 6098 882
Size: 617 2604 1995 2219
Fragments arranged by size:
2604 2219 1995 617
BsaAI yAC'GTr
Cuts at: 493 1204 4096 5226 493
Size: 711 2892 1130 2702
Fragments arranged by size:
2892 2702 1130 711
BsaBI GATnn'nnATC
Cuts at: 7429 7429
Size: 7435
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2009 4440 5242 5624
Size: 53 83 186 936 476 2431 802 382
Cuts at: 5624 6259 275
Size: 635 1451
Fragments arranged by size:
2431 1451 936 802 635 476 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 5868 6266 6540 6687 7098 5868
Size: 398 274 147 411 6205
Fragments arranged by size:
6205 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3869 3899 4598 4628 1178
Size: 30 165 30 2466 30 699 30 3985
Fragments arranged by size:
3985 2466 699 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2023 2124 2630 2855 3705 3959 4004 4019
Size: 837 101 506 225 850 254 45 15
Cuts at: 4019 4040 1186
Size: 21 4581
Fragments arranged by size:
4581 850 837 506 254 225 101 45
21 15
BseYI C'CCAG_C
Cuts at: 3283 4561 6638 7134 3283
Size: 1278 2077 496 3584
Fragments arranged by size:
3584 2077 1278 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2081 3737 3821 4070 4118 1163
Size: 573 345 1656 84 249 48 4480
Fragments arranged by size:
4480 1656 573 345 249 84 48
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4885 5646 6674 1540
Size: 275 186 2884 761 1028 2301
Fragments arranged by size:
2884 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2865 3879 5496 5581 6652 2865
Size: 1014 1617 85 1071 3648
Fragments arranged by size:
3648 1617 1071 1014 85
BsmI GAATG_Cn'
Cuts at: 3064 3686 4648 4946 5039 3064
Size: 622 962 298 93 5460
Fragments arranged by size:
5460 962 622 298 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3626 3626
Size: 7435
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2235 2635 2690 2865 3697 3732 3879
Size: 399 445 400 55 175 832 35 147
Cuts at: 3879 3982 4184 4249 4416 4754 5496 5581 6652
Size: 103 202 65 167 338 742 85 1071
Cuts at: 6652 1391
Size: 2174
Fragments arranged by size:
2174 1071 832 742 445 400 399 338
202 175 167 147 103 85 65 55
35
BspEI T'CCGG_A
Cuts at: 7098 7098
Size: 7435
BspHI T'CATG_A
Cuts at: 5324 7054 5324
Size: 1730 5705
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3040 3566 4092 4357 4790 844
Size: 2196 526 526 265 433 3489
Fragments arranged by size:
3489 2196 526 526 433 265
BsrBI CCG'CTC
Cuts at: 1394 1823 2025 2143 5322 1394
Size: 429 202 118 3179 3507
Fragments arranged by size:
3507 3179 429 202 118
BsrDI GCAATG_nn'
Cuts at: 65 1248 2370 3351 4155 4156 4212 5930 6104
Size: 1183 1122 981 804 1 56 1718 174
Cuts at: 6104 65
Size: 1396
Fragments arranged by size:
1718 1396 1183 1122 981 804 174 56
1
BsrFI r'CCGG_y
Cuts at: 1700 1810 2445 3399 3815 4112 4508 6079 6266
Size: 110 635 954 416 297 396 1571 187
Cuts at: 6266 1700
Size: 2869
Fragments arranged by size:
2869 1571 954 635 416 396 297 187
110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7435
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7435
BssSI C'ACGA_G
Cuts at: 2121 5495 6507 2121
Size: 3374 1012 3049
Fragments arranged by size:
3374 3049 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2685 3556 3681 794
Size: 1891 871 125 4548
Fragments arranged by size:
4548 1891 871 125
BstBI TT'CG_AA
Cuts at: 2263 3223 6239 2263
Size: 960 3016 3459
Fragments arranged by size:
3459 3016 960
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7435
BstXI CCAn_nnnn'nTGG
Cuts at: 3766 4333 3766
Size: 567 6868
BstYI r'GATC_y
Cuts at: 1972 2527 4765 5132 5517 5534 6986 7072 7084
Size: 555 2238 367 385 17 1452 86 12
Cuts at: 7084 7430 1972
Size: 346 1977
Fragments arranged by size:
2238 1977 1452 555 385 367 346 86
17 12
BstZ17I GTA'TAC
Cuts at: 2538 2538
Size: 7435
BtgI C'CryG_G
Cuts at: 513 1065 2004 2046 3999 4383 4443 513
Size: 552 939 42 1953 384 60 3505
Fragments arranged by size:
3505 1953 939 552 384 60 42
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4566 487
Size: 683 56 3340 3356
Fragments arranged by size:
3356 3340 683 56
BtsI GCAGTG_nn'
Cuts at: 2626 2722 4951 5745 5765 2626
Size: 96 2229 794 20 4296
Fragments arranged by size:
4296 2229 794 96 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7435
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7400
DraI TTT'AAA
Cuts at: 4739 4739
Size: 7435
DraIII CAC_nnn'GTG
Cuts at: 2563 2891 4479 2563
Size: 328 1588 5519
Fragments arranged by size:
5519 1588 328
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6442 809
Size: 507 5126 1802
Fragments arranged by size:
5126 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4882 5771 7400
Size: 54 1425 50 275 18 3052 889 1629
Cuts at: 7400 8
Size: 43
Fragments arranged by size:
3052 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4882 1537
Size: 275 3070 4090
Fragments arranged by size:
4090 3070 275
EarI CTCTTCn'nnn_
Cuts at: 1353 5365 1353
Size: 4012 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6022 6396 6542 387
Size: 1064 138 4433 374 146 1280
Fragments arranged by size:
4433 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2863 2863
Size: 7435
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2709 2759 2819 2891 3158 3551 3592
Size: 393 1077 50 60 72 267 393 41
Cuts at: 3592 3606 3718 4043 4339 5498 6095 6882 7150
Size: 14 112 325 296 1159 597 787 268
Cuts at: 7150 1239
Size: 1524
Fragments arranged by size:
1524 1159 1077 787 597 393 393 325
296 268 267 112 72 60 50 41
14
EcoNI CCTnn'n_nnAGG
Cuts at: 2870 2870
Size: 7435
EcoO109I rG'GnC_Cy
Cuts at: 2042 2631 2632 4315 2042
Size: 589 1 1683 5162
Fragments arranged by size:
5162 1683 589 1
EcoRI G'AATT_C
Cuts at: 4744 5122 4744
Size: 378 7057
EcoRV GAT'ATC
Cuts at: 4465 4465
Size: 7435
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3767 3913 3767
Size: 146 7289
FspI TGC'GCA
Cuts at: 4148 4601 5941 4148
Size: 453 1340 5642
Fragments arranged by size:
5642 1340 453
HaeII r_GCGC'y
Cuts at: 1678 4668 4687 6582 1678
Size: 2990 19 1895 2531
Fragments arranged by size:
2990 2531 1895 19
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2034 2066 3404 3436 6076
Size: 32 135 32 462 32 1338 32 2640
Cuts at: 6076 6108 6150 6182 1373
Size: 32 42 32 2626
Fragments arranged by size:
2640 2626 1338 462 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4779 5025 669
Size: 988 281 2841 246 3079
Fragments arranged by size:
3079 2841 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3230 748
Size: 2482 4953
HpaI GTT'AAC
Cuts at: 5025 5025
Size: 7435
KpnI G_GTAC'C
Cuts at: 2832 4243 4341 4760 2832
Size: 1411 98 419 5507
Fragments arranged by size:
5507 1411 419 98
MfeI C'AATT_G
Cuts at: 5034 5034
Size: 7435
MluI A'CGCG_T
Cuts at: 7311 7311
Size: 7435
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3148 4104 6549 6733 1305
Size: 1843 956 2445 184 2007
Fragments arranged by size:
2445 2007 1843 956 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5613
Fragments arranged by size:
5613 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2078 2844 3074 3432 4364 4409 4503
Size: 648 912 766 230 358 932 45 94
Cuts at: 4503 5393 5752 5911 518
Size: 890 359 159 2042
Fragments arranged by size:
2042 932 912 890 766 648 359 358
230 159 94 45
MspA1I CmG'CkG
Cuts at: 1606 2048 2065 3571 4851 5528 6676 6921 1606
Size: 442 17 1506 1280 677 1148 245 2120
Fragments arranged by size:
2120 1506 1280 1148 677 442 245 17
NaeI GCC'GGC
Cuts at: 3817 4510 3817
Size: 693 6742
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5944
Fragments arranged by size:
5944 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7435
NgoMIV G'CCGG_C
Cuts at: 3815 4508 3815
Size: 693 6742
NheI G'CTAG_C
Cuts at: 1052 4043 1052
Size: 2991 4444
NotI GC'GGCC_GC
Cuts at: 4882 4882
Size: 7435
NruI TCG'CGA
Cuts at: 2767 2767
Size: 7435
NspI r_CATG'y
Cuts at: 1132 1229 2609 3202 4147 4286 4793 6338 1132
Size: 97 1380 593 945 139 507 1545 2229
Fragments arranged by size:
2229 1545 1380 945 593 507 139 97
PasI CC'CwG_GG
Cuts at: 1348 2235 2317 1348
Size: 887 82 6466
Fragments arranged by size:
6466 887 82
PciI A'CATG_T
Cuts at: 3198 4282 6334 3198
Size: 1084 2052 4299
Fragments arranged by size:
4299 2052 1084
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7435
PfoI T'CCnGG_A
Cuts at: 2523 2523
Size: 7435
PmeI GTTT'AAAC
Cuts at: 4739 4739
Size: 7435
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4598 4630 5504 5536 7017 7049 4598
Size: 32 874 32 1481 32 4984
Fragments arranged by size:
4984 1481 874 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2042 4315 2042
Size: 2273 5162
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7435
PsiI TTA'TAA
Cuts at: 5005 5005
Size: 7435
PspOMI G'GGCC_C
Cuts at: 2631 3978 2631
Size: 1347 6088
PstI C_TGCA'G
Cuts at: 830 2743 3451 3777 4533 4787 830
Size: 1913 708 326 756 254 3478
Fragments arranged by size:
3478 1913 756 708 326 254
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7435
SacI G_AGCT'C
Cuts at: 2865 2865
Size: 7435
SacII CC_GC'GG
Cuts at: 2049 2049
Size: 7435
SalI G'TCGA_C
Cuts at: 1936 4777 1936
Size: 2841 4594
SanDI GG'GwC_CC
Cuts at: 2042 2042
Size: 7435
SbfI CC_TGCA'GG
Cuts at: 4787 4787
Size: 7435
ScaI AGT'ACT
Cuts at: 5683 7395 5683
Size: 1712 5723
SexAI A'CCwGG_T
Cuts at: 2655 2655
Size: 7435
SfcI C'TryA_G
Cuts at: 826 1045 2739 3447 3773 4173 4529 4726 4783
Size: 219 1694 708 326 400 356 197 57
Cuts at: 4783 5918 6599 6790 826
Size: 1135 681 191 1471
Fragments arranged by size:
1694 1471 1135 708 681 400 356 326
219 197 191 57
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7435
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7435
SmaI CCC'GGG
Cuts at: 1872 2921 3212 4762 1872
Size: 1049 291 1550 4545
Fragments arranged by size:
4545 1550 1049 291
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2250 2531 4377 5539 6440 6702
Size: 197 926 307 281 1846 1162 901 262
Cuts at: 6702 820
Size: 1553
Fragments arranged by size:
1846 1553 1162 926 901 307 281 262
197
SnaBI TAC'GTA
Cuts at: 493 5226 493
Size: 4733 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7435
SphI G_CATG'C
Cuts at: 1132 4793 1132
Size: 3661 3774
SspI AAT'ATT
Cuts at: 5 52 5359 5
Size: 47 5307 2081
Fragments arranged by size:
5307 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7435
StyI C'CwwG_G
Cuts at: 513 1065 2004 2344 2552 3039 3759 3888 4545
Size: 552 939 340 208 487 720 129 657
Cuts at: 4545 4874 513
Size: 329 3074
Fragments arranged by size:
3074 939 720 657 552 487 340 329
208 129
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3167 4082 4133 5462 5479 5632 5817
Size: 1255 1587 915 51 1329 17 153 185
Cuts at: 5817 6262 325
Size: 445 1498
Fragments arranged by size:
1587 1498 1329 1255 915 445 185 153
51 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5681 7393 96
Size: 274 80 33 51 1443 3704 1712 138
Fragments arranged by size:
3704 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2482 2505 2514 2537 3869 3901 7395 7427 2482
Size: 23 9 23 1332 32 3494 32 2490
Fragments arranged by size:
3494 2490 1332 32 32 23 23 9
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7435
XbaI T'CTAG_A
Cuts at: 4771 4771
Size: 7435
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3007 4552 3007
Size: 1545 5890
XhoI C'TCGA_G
Cuts at: 1943 2250 1943
Size: 307 7128
XmaI C'CCGG_G
Cuts at: 1870 2919 3210 4760 1870
Size: 1049 291 1550 4545
Fragments arranged by size:
4545 1550 1049 291
XmnI GAAnn'nnTTC
Cuts at: 3219 4613 5564 3219
Size: 1394 951 5090
Fragments arranged by size:
5090 1394 951
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5243 276
Size: 53 83 186 936 3709 2468
Fragments arranged by size:
3709 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NruI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacI
SacII SalI SanDI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbeI BfrBI BplI BsiWI
Bsu36I FseI FspAI KasI NarI NsiI PacI PmlI
PspXI PsrI PvuII RsrII SapI SfiI SfoI SrfI
SwaI