Restriction map for
pFN21AB4546
(Circular) (Six-base) MAPSORT of: pFN21AB4546.seq Check: 3787 from: 1 to: 15085
pFN21AB4546 15085 bp
With 182 enzymes: SgfI *
February 2, 2017 15:09 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2418 2458 5139 5511 6724 2418
Size: 40 2681 372 1213 10779
Fragments arranged by size:
10779 2681 1213 372 40
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 12895 278
Size: 53 83 186 936 11359 2468
Fragments arranged by size:
11359 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3669 3873 7194 8382 12428 1937
Size: 1732 204 3321 1188 4046 4594
Fragments arranged by size:
4594 4046 3321 1732 1188 204
Acc65I G'GTAC_C
Cuts at: 5163 8325 12406 5163
Size: 3162 4081 7842
Fragments arranged by size:
7842 4081 3162
AclI AA'CG_TT
Cuts at: 1563 8082 13212 13585 1563
Size: 6519 5130 373 3063
Fragments arranged by size:
6519 5130 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3581 4221 5314 5453 5515 5951 6537 7015
Size: 1949 640 1093 139 62 436 586 478
Cuts at: 7015 7479 7995 8771 10009 10104 10275 10688 10700
Size: 464 516 776 1238 95 171 413 12
Cuts at: 10700 11052 13148 14532 14800 1632
Size: 352 2096 1384 268 1917
Fragments arranged by size:
2096 1949 1917 1384 1238 1093 776 640
586 516 478 464 436 413 352 268
171 139 95 62 12
AfeI AGC'GCT
Cuts at: 2046 10055 2046
Size: 8009 7076
AflII C'TTAA_G
Cuts at: 820 1017 10603 820
Size: 197 9586 5302
Fragments arranged by size:
9586 5302 197
AflIII A'CryG_T
Cuts at: 1203 3987 5109 9453 10098 11676 13984 14961 1203
Size: 2784 1122 4344 645 1578 2308 977 1327
Fragments arranged by size:
4344 2784 2308 1578 1327 1122 977 645
AgeI A'CCGG_T
Cuts at: 3410 11732 13916 3410
Size: 8322 2184 4579
Fragments arranged by size:
8322 4579 2184
AhdI GACnn_n'nnGTC
Cuts at: 6658 13814 6658
Size: 7156 7929
AleI CACnn'nnGTG
Cuts at: 1166 8301 1166
Size: 7135 7950
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2733 9050 1184
Size: 1549 6317 7219
Fragments arranged by size:
7219 6317 1549
AlwNI CAG_nnn'CTG
Cuts at: 2019 2789 4651 5192 5570 6249 7782 8919 9992
Size: 770 1862 541 378 679 1533 1137 1073
Cuts at: 9992 12828 14400 2019
Size: 2836 1572 2704
Fragments arranged by size:
2836 2704 1862 1572 1533 1137 1073 770
679 541 378
ApaI G_GGCC'C
Cuts at: 2026 7899 10565 2026
Size: 5873 2666 6546
Fragments arranged by size:
6546 5873 2666
ApaLI G'TGCA_C
Cuts at: 2122 2567 4054 8279 13142 14298 2122
Size: 445 1487 4225 4863 1156 2909
Fragments arranged by size:
4863 4225 2909 1487 1156 445
ApoI r'AATT_y
Cuts at: 1493 2470 3272 3450 3466 3614 4031 4512 4931
Size: 977 802 178 16 148 417 481 419
Cuts at: 4931 6830 7435 7694 8129 10323 10761 12316 12394
Size: 1899 605 259 435 2194 438 1555 78
Cuts at: 12394 12620 12772 14881 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2194 2109 1899 1697 1555 977 802 605
481 438 435 419 417 259 226 178
152 148 78 16
AseI AT'TA_AT
Cuts at: 160 1032 8555 13639 160
Size: 872 7523 5084 1606
Fragments arranged by size:
7523 5084 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 15085
AvaI C'yCGr_G
Cuts at: 1870 1943 5874 9533 11331 11979 12410 1870
Size: 73 3931 3659 1798 648 431 4545
Fragments arranged by size:
4545 3931 3659 1798 648 431 73
BamHI G'GATC_C
Cuts at: 6168 8113 9288 10796 11628 12415 12782 6168
Size: 1945 1175 1508 832 787 367 8471
Fragments arranged by size:
8471 1945 1508 1175 832 787 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4884 5163 5499 6404 7305
Size: 325 217 627 3097 279 336 905 901
Cuts at: 7305 7680 7969 8157 8325 9026 9225 9750 11735
Size: 375 289 188 168 701 199 525 1985
Cuts at: 11735 12406 13861 618
Size: 671 1455 1842
Fragments arranged by size:
3097 1985 1842 1455 905 901 701 671
627 525 375 336 325 289 279 217
199 188 168
BanII G_rGCy'C
Cuts at: 1391 2026 2551 2911 4366 4467 6075 7899 9580
Size: 635 525 360 1455 101 1608 1824 1681
Cuts at: 9580 10565 12339 12404 1391
Size: 985 1774 65 4072
Fragments arranged by size:
4072 1824 1774 1681 1608 1455 985 635
525 360 101 65
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3352 6716 7911 8774 9061 10116 10676
Size: 971 1453 3364 1195 863 287 1055 560
Cuts at: 10676 12364 928
Size: 1688 3649
Fragments arranged by size:
3649 3364 1688 1453 1195 1055 971 863
560 287
BbvCI CC'TCA_GC
Cuts at: 2066 2066
Size: 15085
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6623 6657 10760 10794 13276 13310 1903
Size: 34 4686 34 4103 34 2482 34 3678
Fragments arranged by size:
4686 4103 3678 2482 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3526 5365 6618 6904 7873 10625 11348 11714 12977
Size: 1839 1253 286 969 2752 723 366 1263
Cuts at: 12977 14187 3526
Size: 1210 4424
Fragments arranged by size:
4424 2752 1839 1263 1253 1210 969 723
366 286
BclI T'GATC_A
Cuts at: 1548 3051 4369 11082 12250 1548
Size: 1503 1318 6713 1168 4383
Fragments arranged by size:
6713 4383 1503 1318 1168
BfrBI ATG'CAT
Cuts at: 2710 9477 2710
Size: 6767 8318
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3032 6770 13696
Size: 107 122 71 631 605 1360 3738 6926
Cuts at: 13696 136
Size: 1525
Fragments arranged by size:
6926 3738 1525 1360 631 605 122 107
71
BglII A'GATC_T
Cuts at: 3232 3837 7561 7631 9208 10278 15080 3232
Size: 605 3724 70 1577 1070 4802 3237
Fragments arranged by size:
4802 3724 3237 1577 1070 605 70
BlpI GC'TnA_GC
Cuts at: 6377 12502 6377
Size: 6125 8960
Bme1580I G_kGCm'C
Cuts at: 1790 2026 2126 2459 2571 4058 5105 6409 7310
Size: 236 100 333 112 1487 1047 1304 901
Cuts at: 7310 7899 7972 8283 8907 10565 11131 11718 13146
Size: 589 73 311 624 1658 566 587 1428
Cuts at: 13146 14302 1790
Size: 1156 2573
Fragments arranged by size:
2573 1658 1487 1428 1304 1156 1047 901
624 589 587 566 333 311 236 112
100 73
BmgBI CAC'GTC
Cuts at: 1318 8197 1318
Size: 6879 8206
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3095 4340 5684
Size: 416 53 83 399 24 1678 1245 1344
Cuts at: 5684 6237 6760 8389 8708 9970 10031 10870 11470
Size: 553 523 1629 319 1262 61 839 600
Cuts at: 11470 11796 11860 12377 12816 13774 442
Size: 326 64 517 439 958 1753
Fragments arranged by size:
1753 1678 1629 1344 1262 1245 958 839
600 553 523 517 439 416 399 326
319 83 64 61 53 24
BmtI G_CTAG'C
Cuts at: 1056 5510 1056
Size: 4454 10631
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6199 6199
Size: 15085
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2706 3979 4298 4454 4874 5056 5576 5940
Size: 1467 1273 319 156 420 182 520 364
Cuts at: 5940 6040 7040 8284 8858 11242 12206 13745 1239
Size: 100 1000 1244 574 2384 964 1539 2579
Fragments arranged by size:
2579 2384 1539 1467 1273 1244 1000 964
574 520 420 364 319 182 156 100
Bpu10I CC'TnA_GC
Cuts at: 2066 7321 2066
Size: 5255 9830
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4717 5043 11545 13210 14075 14373 4717
Size: 326 6502 1665 865 298 5429
Fragments arranged by size:
6502 5429 1665 865 326 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6141 6953 8646 9530 11107 11849 13748
Size: 617 4642 812 1693 884 1577 742 1899
Cuts at: 13748 882
Size: 2219
Fragments arranged by size:
4642 2219 1899 1693 1577 884 812 742
617
BsaAI yAC'GTr
Cuts at: 493 1204 2401 4356 9097 11677 12876 493
Size: 711 1197 1955 4741 2580 1199 2702
Fragments arranged by size:
4741 2702 2580 1955 1199 1197 711
BsaBI GATnn'nnATC
Cuts at: 3231 5856 10830 15079 3231
Size: 2625 4974 4249 3237
Fragments arranged by size:
4974 4249 3237 2625
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2958 4267 5460 9644
Size: 53 83 186 936 1425 1309 1193 4184
Cuts at: 9644 12892 13274 13909 275
Size: 3248 382 635 1451
Fragments arranged by size:
4184 3248 1451 1425 1309 1193 936 635
382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3410 10495 11732 13518 13916 14190 14337 14748 3410
Size: 7085 1237 1786 398 274 147 411 3747
Fragments arranged by size:
7085 3747 1786 1237 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2609 2639 3126 3156 3840
Size: 30 165 30 1206 30 487 30 684
Cuts at: 3840 3870 5262 5292 6870 6900 7201 7231 8727
Size: 30 1392 30 1578 30 301 30 1496
Cuts at: 8727 8757 10040 10070 11585 11615 1178
Size: 30 1283 30 1515 30 4648
Fragments arranged by size:
4648 1578 1515 1496 1392 1283 1206 684
487 301 165 30 30 30 30 30
30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3868 4156 4993 5468 8281 8417 11317 11593
Size: 2682 288 837 475 2813 136 2900 276
Cuts at: 11593 1186
Size: 4678
Fragments arranged by size:
4678 2900 2813 2682 837 475 288 276
136
BseYI C'CCAG_C
Cuts at: 2019 2053 2526 2843 3030 4096 5238 5339 8228
Size: 34 473 317 187 1066 1142 101 2889
Cuts at: 8228 10044 11168 11661 12129 14288 14784 2019
Size: 1816 1124 493 468 2159 496 2320
Fragments arranged by size:
2889 2320 2159 1816 1142 1124 1066 496
493 473 468 317 187 101 34
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2401 4378 5134 6294 7491 8279 8702
Size: 573 665 1977 756 1160 1197 788 423
Cuts at: 8702 9478 10352 10764 11710 1163
Size: 776 874 412 946 4538
Fragments arranged by size:
4538 1977 1197 1160 946 874 788 776
756 665 573 423 412
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 11733 12535 13296 14324 1540
Size: 275 186 9732 802 761 1028 2301
Fragments arranged by size:
9732 2301 1028 802 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2126 2172 2551 2571 4058 4355 7322 8223 8283
Size: 46 379 20 1487 297 2967 901 60
Cuts at: 8283 8745 9580 10134 11122 13146 13231 14302 2126
Size: 462 835 554 988 2024 85 1071 2909
Fragments arranged by size:
2967 2909 2024 1487 1071 988 901 835
554 462 379 297 85 60 46 20
BsmI GAATG_Cn'
Cuts at: 3830 4907 5183 6757 8020 9269 12596 12689 3830
Size: 1077 276 1574 1263 1249 3327 93 6226
Fragments arranged by size:
6226 3327 1574 1263 1249 1077 276 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2026 2126 2172 2459 2551 2571 2911
Size: 399 236 100 46 287 92 20 340
Cuts at: 2911 4058 4355 4366 4467 5105 6075 6409 7310
Size: 1147 297 11 101 638 970 334 901
Cuts at: 7310 7322 7899 7972 8223 8283 8745 8907 9580
Size: 12 577 73 251 60 462 162 673
Cuts at: 9580 10134 10565 11122 11131 11718 12339 12404 13146
Size: 554 431 557 9 587 621 65 742
Cuts at: 13146 13231 14302 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1147 1071 970 901 742 673 638
621 587 577 557 554 462 431 399
340 334 297 287 251 236 162 101
100 92 85 73 65 60 46 20
12 11 9
BspEI T'CCGG_A
Cuts at: 14748 14748
Size: 15085
BspHI T'CATG_A
Cuts at: 3472 4459 4584 5977 8691 10207 12247 12974 14704
Size: 987 125 1393 2714 1516 2040 727 1730
Cuts at: 14704 3472
Size: 3853
Fragments arranged by size:
3853 2714 2040 1730 1516 1393 987 727
125
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2418 2458 5139 5511 6572 6702 6724 8814
Size: 1574 40 2681 372 1061 130 22 2090
Cuts at: 8814 9834 12440 844
Size: 1020 2606 3489
Fragments arranged by size:
3489 2681 2606 2090 1574 1061 1020 372
130 40 22
BsrBI CCG'CTC
Cuts at: 1394 1823 12972 1394
Size: 429 11149 3507
Fragments arranged by size:
11149 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2722 3266 5838 8821 8829 11095 13580
Size: 1183 1474 544 2572 2983 8 2266 2485
Cuts at: 13580 13754 65
Size: 174 1396
Fragments arranged by size:
2983 2572 2485 2266 1474 1396 1183 544
174 8
BsrFI r'CCGG_y
Cuts at: 1700 1810 3410 8345 10070 11732 13729 13916 1700
Size: 110 1600 4935 1725 1662 1997 187 2869
Fragments arranged by size:
4935 2869 1997 1725 1662 1600 187 110
BsrGI T'GTAC_A
Cuts at: 96 6222 7048 7571 10154 11679 96
Size: 6126 826 523 2583 1525 3502
Fragments arranged by size:
6126 3502 2583 1525 826 523
BssHII G'CGCG_C
Cuts at: 1925 2072 2074 1925
Size: 147 2 14936
Fragments arranged by size:
14936 147 2
BssSI C'ACGA_G
Cuts at: 2748 8649 8773 10049 13145 14157 2748
Size: 5901 124 1276 3096 1012 3676
Fragments arranged by size:
5901 3676 3096 1276 1012 124
BstAPI GCAn_nnn'nTGC
Cuts at: 794 5178 6042 6564 8164 11508 794
Size: 4384 864 522 1600 3344 4371
Fragments arranged by size:
4384 4371 3344 1600 864 522
BstBI TT'CG_AA
Cuts at: 8523 13889 8523
Size: 5366 9719
BstEII G'GTnAC_C
Cuts at: 1184 2366 11405 1184
Size: 1182 9039 4864
Fragments arranged by size:
9039 4864 1182
BstXI CCAn_nnnn'nTGG
Cuts at: 2363 2658 3736 4633 8364 8818 9668 11176 11968
Size: 295 1078 897 3731 454 850 1508 792
Cuts at: 11968 2363
Size: 5480
Fragments arranged by size:
5480 3731 1508 1078 897 850 792 454
295
BstYI r'GATC_y
Cuts at: 1972 3232 3837 6168 7561 7631 8113 8306 9208
Size: 1260 605 2331 1393 70 482 193 902
Cuts at: 9208 9288 9700 10278 10796 10831 11628 11971 12415
Size: 80 412 578 518 35 797 343 444
Cuts at: 12415 12782 13167 13184 14636 14722 14734 15080 1972
Size: 367 385 17 1452 86 12 346 1977
Fragments arranged by size:
2331 1977 1452 1393 1260 902 797 605
578 518 482 444 412 385 367 346
343 193 86 80 70 35 17 12
Bsu36I CC'TnA_GG
Cuts at: 2952 3425 7300 2952
Size: 473 3875 10737
Fragments arranged by size:
10737 3875 473
BtgI C'CryG_G
Cuts at: 513 1065 2004 2131 3184 3399 3793 4192 4870
Size: 552 939 127 1053 215 394 399 678
Cuts at: 4870 6259 8109 9466 9639 10591 11911 513
Size: 1389 1850 1357 173 952 1320 3687
Fragments arranged by size:
3687 1850 1389 1357 1320 1053 952 939
678 552 399 394 215 173 127
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 12109 487
Size: 683 56 10883 3463
Fragments arranged by size:
10883 3463 683 56
BtsI GCAGTG_nn'
Cuts at: 2020 2389 5105 5122 6259 7749 9466 10236 10541
Size: 369 2716 17 1137 1490 1717 770 305
Cuts at: 10541 10776 12084 12601 13395 13415 2020
Size: 235 1308 517 794 20 3690
Fragments arranged by size:
3690 2716 1717 1490 1308 1137 794 770
517 369 305 235 20 17
ClaI AT'CG_AT
Cuts at: 1554 5860 7842 8928 10545 1554
Size: 4306 1982 1086 1617 6094
Fragments arranged by size:
6094 4306 1982 1617 1086
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2966 3001 5048 5083 529
Size: 35 2402 35 2047 35 10531
Fragments arranged by size:
10531 2402 2047 35 35 35
DraI TTT'AAA
Cuts at: 7528 11371 12389 7528
Size: 3843 1018 10224
Fragments arranged by size:
10224 3843 1018
DraIII CAC_nnn'GTG
Cuts at: 10755 12067 10755
Size: 1312 13773
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 14092 809
Size: 507 12776 1802
Fragments arranged by size:
12776 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4227 4687 4813
Size: 54 1425 50 275 18 2397 460 126
Cuts at: 4813 6256 9463 11288 12532 13421 15050 8
Size: 1443 3207 1825 1244 889 1629 43
Fragments arranged by size:
3207 2397 1825 1629 1443 1425 1244 889
460 275 126 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 12532 1537
Size: 275 10720 4090
Fragments arranged by size:
10720 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 5314 7095 9549 11497 13015 1353
Size: 3961 1781 2454 1948 1518 3423
Fragments arranged by size:
3961 3423 2454 1948 1781 1518
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5430 13672 14046 14192 387
Size: 1064 138 3841 8242 374 146 1280
Fragments arranged by size:
8242 3841 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2549 9578 2549
Size: 7029 8056
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2706 3581 3979 4221 4298 4454 4874
Size: 393 1074 875 398 242 77 156 420
Cuts at: 4874 5056 5314 5453 5515 5576 5940 5951 6040
Size: 182 258 139 62 61 364 11 89
Cuts at: 6040 6537 7015 7040 7479 7995 8284 8771 8858
Size: 497 478 25 439 516 289 487 87
Cuts at: 8858 10009 10104 10275 10688 10700 11052 11242 12206
Size: 1151 95 171 413 12 352 190 964
Cuts at: 12206 13148 13745 14532 14800 1239
Size: 942 597 787 268 1524
Fragments arranged by size:
1524 1151 1074 964 942 875 787 597
516 497 487 478 439 420 413 398
393 364 352 289 268 258 242 190
182 171 156 139 95 89 87 77
62 61 25 12 11
EcoNI CCTnn'n_nnAGG
Cuts at: 2951 4380 6698 2951
Size: 1429 2318 11338
Fragments arranged by size:
11338 2318 1429
EcoO109I rG'GnC_Cy
Cuts at: 4240 5365 7224 9354 10562 4240
Size: 1125 1859 2130 1208 8763
Fragments arranged by size:
8763 2130 1859 1208 1125
EcoRI G'AATT_C
Cuts at: 4512 10761 12394 12772 4512
Size: 6249 1633 378 6825
Fragments arranged by size:
6825 6249 1633 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4922 5213 6567 10705 4922
Size: 291 1354 4138 9302
Fragments arranged by size:
9302 4138 1354 291
FspI TGC'GCA
Cuts at: 8636 13591 8636
Size: 4955 10130
HaeII r_GCGC'y
Cuts at: 1678 2048 3802 10035 10057 14232 1678
Size: 370 1754 6233 22 4175 2531
Fragments arranged by size:
6233 4175 2531 1754 370 22
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2328 2360 2609 2641 3155
Size: 32 135 32 756 32 249 32 514
Cuts at: 3155 3187 3248 3280 3285 3317 3840 3872 4097
Size: 32 61 32 5 32 523 32 225
Cuts at: 4097 4129 6431 6463 6478 6510 6644 6676 6887
Size: 32 2302 32 15 32 134 32 211
Cuts at: 6887 6919 7468 7500 7764 7796 8090 8122 9675
Size: 32 549 32 264 32 294 32 1553
Cuts at: 9675 9707 9895 9927 10648 10680 11145 11177 11432
Size: 32 188 32 721 32 465 32 255
Cuts at: 11432 11464 11585 11617 11762 11794 13726 13758 13800
Size: 32 121 32 145 32 1932 32 42
Cuts at: 13800 13832 1373
Size: 32 2626
Fragments arranged by size:
2626 2302 1932 1553 756 721 549 523
514 465 294 264 255 249 225 211
188 145 135 134 121 61 42 32
32 32 32 32 32 32 32 32
32 32 32 32 32 32 32 32
32 32 32 32 32 32 32 32
15 5
HincII GTy'rAC
Cuts at: 669 1657 1938 8735 11554 12100 12429 12675 669
Size: 988 281 6797 2819 546 329 246 3079
Fragments arranged by size:
6797 3079 2819 988 546 329 281 246
HindIII A'AGCT_T
Cuts at: 748 4144 10480 748
Size: 3396 6336 5353
Fragments arranged by size:
6336 5353 3396
HpaI GTT'AAC
Cuts at: 12675 12675
Size: 15085
KpnI G_GTAC'C
Cuts at: 5167 8329 12410 5167
Size: 3162 4081 7842
Fragments arranged by size:
7842 4081 3162
MfeI C'AATT_G
Cuts at: 3996 7909 11174 11667 12684 3996
Size: 3913 3265 493 1017 6397
Fragments arranged by size:
6397 3913 3265 1017 493
MluI A'CGCG_T
Cuts at: 14961 14961
Size: 15085
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2576 5726 6394 6630 7139 8498 9572 10267
Size: 1271 3150 668 236 509 1359 1074 695
Cuts at: 10267 10340 10463 10914 14199 14383 1305
Size: 73 123 451 3285 184 2007
Fragments arranged by size:
3285 3150 2007 1359 1271 1074 695 668
509 451 236 184 123 73
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4229 4689 4815 6258 9465
Size: 54 1425 343 2397 460 126 1443 3207
Cuts at: 9465 11290 10
Size: 1825 3805
Fragments arranged by size:
3805 3207 2397 1825 1443 1425 460 343
126 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2715 2852 3189 3477 4959 5210 6730
Size: 648 1549 137 337 288 1482 251 1520
Cuts at: 6730 8301 8642 9032 9452 9666 10103 13043 13402
Size: 1571 341 390 420 214 437 2940 359
Cuts at: 13402 13561 518
Size: 159 2042
Fragments arranged by size:
2940 2042 1571 1549 1520 1482 648 437
420 390 359 341 337 288 251 214
159 137
MspA1I CmG'CkG
Cuts at: 1606 5034 5353 5567 5598 8111 9921 11587 11625
Size: 3428 319 214 31 2513 1810 1666 38
Cuts at: 11625 11712 11896 12036 12501 13178 14326 14571 1606
Size: 87 184 140 465 677 1148 245 2120
Fragments arranged by size:
3428 2513 2120 1810 1666 1148 677 465
319 245 214 184 140 87 38 31
NaeI GCC'GGC
Cuts at: 8347 10072 8347
Size: 1725 13360
NcoI C'CATG_G
Cuts at: 513 1065 2004 3184 3399 3793 6259 9466 10591
Size: 552 939 1180 215 394 2466 3207 1125
Cuts at: 10591 11911 513
Size: 1320 3687
Fragments arranged by size:
3687 3207 2466 1320 1180 1125 939 552
394 215
NdeI CA'TA_TG
Cuts at: 387 2740 5144 387
Size: 2353 2404 10328
Fragments arranged by size:
10328 2404 2353
NgoMIV G'CCGG_C
Cuts at: 8345 10070 8345
Size: 1725 13360
NheI G'CTAG_C
Cuts at: 1052 5506 1052
Size: 4454 10631
NotI GC'GGCC_GC
Cuts at: 12532 12532
Size: 15085
NsiI A_TGCA'T
Cuts at: 2712 9479 2712
Size: 6767 8318
NspI r_CATG'y
Cuts at: 1132 1229 3991 5113 8641 8672 9457 10102 10534
Size: 97 2762 1122 3528 31 785 645 432
Cuts at: 10534 12022 12122 12443 13988 1132
Size: 1488 100 321 1545 2229
Fragments arranged by size:
3528 2762 2229 1545 1488 1122 785 645
432 321 100 97 31
PasI CC'CwG_GG
Cuts at: 1348 6467 8919 1348
Size: 5119 2452 7514
Fragments arranged by size:
7514 5119 2452
PciI A'CATG_T
Cuts at: 3987 5109 9453 10098 13984 3987
Size: 1122 4344 645 3886 5088
Fragments arranged by size:
5088 4344 3886 1122 645
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3980 4685 7121 10981 11179 12067 1108
Size: 2872 705 2436 3860 198 888 4126
Fragments arranged by size:
4126 3860 2872 2436 888 705 198
PfoI T'CCnGG_A
Cuts at: 2212 4431 6171 6666 10627 2212
Size: 2219 1740 495 3961 6670
Fragments arranged by size:
6670 3961 2219 1740 495
PmeI GTTT'AAAC
Cuts at: 12389 12389
Size: 15085
PmlI CAC'GTG
Cuts at: 2401 4356 11677 2401
Size: 1955 7321 5809
Fragments arranged by size:
7321 5809 1955
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 13154 13186 14667 14699 13154
Size: 32 1481 32 13540
Fragments arranged by size:
13540 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 5365 7224 9354 5365
Size: 1859 2130 11096
Fragments arranged by size:
11096 2130 1859
PshAI GACnn'nnGTC
Cuts at: 1613 12364 1613
Size: 10751 4334
PsiI TTA'TAA
Cuts at: 7794 12655 7794
Size: 4861 10224
PspOMI G'GGCC_C
Cuts at: 2022 7895 10561 2022
Size: 5873 2666 6546
Fragments arranged by size:
6546 5873 2666
PspXI vC'TCGA_Gb
Cuts at: 11979 11979
Size: 15085
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 11328 11360 11328
Size: 32 15053
PstI C_TGCA'G
Cuts at: 830 6034 6254 10066 10586 11711 12437 830
Size: 5204 220 3812 520 1125 726 3478
Fragments arranged by size:
5204 3812 3478 1125 726 520 220
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 15085
PvuII CAG'CTG
Cuts at: 5353 5567 11587 11896 12036 5353
Size: 214 6020 309 140 8402
Fragments arranged by size:
8402 6020 309 214 140
SacI G_AGCT'C
Cuts at: 2551 9580 2551
Size: 7029 8056
SacII CC_GC'GG
Cuts at: 8112 8112
Size: 15085
SalI G'TCGA_C
Cuts at: 1936 12427 1936
Size: 10491 4594
SbfI CC_TGCA'GG
Cuts at: 12437 12437
Size: 15085
ScaI AGT'ACT
Cuts at: 4859 5950 5972 10523 10744 13333 15045 4859
Size: 1091 22 4551 221 2589 1712 4899
Fragments arranged by size:
4899 4551 2589 1712 1091 221 22
SexAI A'CCwGG_T
Cuts at: 4489 4842 5588 9350 4489
Size: 353 746 3762 10224
Fragments arranged by size:
10224 3762 746 353
SfcI C'TryA_G
Cuts at: 826 1045 2481 3138 3288 3666 3889 5906 6030
Size: 219 1436 657 150 378 223 2017 124
Cuts at: 6030 6250 6843 7018 8747 8911 9137 10062 10582
Size: 220 593 175 1729 164 226 925 520
Cuts at: 10582 11707 12433 13568 14249 14440 826
Size: 1125 726 1135 681 191 1471
Fragments arranged by size:
2017 1729 1471 1436 1135 1125 925 726
681 657 593 520 378 226 223 220
219 191 175 164 150 124
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 15085
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 15085
SmaI CCC'GGG
Cuts at: 1872 12412 1872
Size: 10540 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4732 5058 5874 10603 11560 11979
Size: 197 926 2789 326 816 4729 957 419
Cuts at: 11979 13189 14090 14352 820
Size: 1210 901 262 1553
Fragments arranged by size:
4729 2789 1553 1210 957 926 901 816
419 326 262 197
SnaBI TAC'GTA
Cuts at: 493 12876 493
Size: 12383 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 15085
SphI G_CATG'C
Cuts at: 1132 12443 1132
Size: 11311 3774
SspI AAT'ATT
Cuts at: 5 52 8953 9797 13009 5
Size: 47 8901 844 3212 2081
Fragments arranged by size:
8901 3212 2081 844 47
StuI AGG'CCT
Cuts at: 1517 2769 1517
Size: 1252 13833
StyI C'CwwG_G
Cuts at: 513 1065 2004 3184 3399 3793 3979 4663 5167
Size: 552 939 1180 215 394 186 684 504
Cuts at: 5167 6131 6259 6874 7310 9466 9778 10591 11764
Size: 964 128 615 436 2156 312 813 1173
Cuts at: 11764 11911 12524 513
Size: 147 613 3074
Fragments arranged by size:
3074 2156 1180 1173 964 939 813 684
615 613 552 504 436 394 312 215
186 147 128
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2141 2586 3885 5199 5371 7928 8378
Size: 1255 561 445 1299 1314 172 2557 450
Cuts at: 8378 9033 9243 9738 13112 13129 13282 13467 13912
Size: 655 210 495 3374 17 153 185 445
Cuts at: 13912 325
Size: 1498
Fragments arranged by size:
3374 2557 1498 1314 1299 1255 655 561
495 450 445 445 210 185 172 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4857 5325 5472
Size: 274 80 33 51 1443 2880 468 147
Cuts at: 5472 5517 5948 5970 6222 7048 7291 7571 9519
Size: 45 431 22 252 826 243 280 1948
Cuts at: 9519 9623 9852 10154 10521 10742 11679 11925 13331
Size: 104 229 302 367 221 937 246 1406
Cuts at: 13331 15043 96
Size: 1712 138
Fragments arranged by size:
2880 1948 1712 1443 1406 937 826 468
431 367 302 280 274 252 246 243
229 221 147 138 104 80 51 45
33 22
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6309 6341 7201 7233 7338 7370 8482 8514 10040
Size: 32 860 32 105 32 1112 32 1526
Cuts at: 10040 10072 10206 10238 10281 10313 10788 10820 15045
Size: 32 134 32 43 32 475 32 4225
Cuts at: 15045 15077 6309
Size: 32 6317
Fragments arranged by size:
6317 4225 1526 1112 860 475 134 105
43 32 32 32 32 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 5390 9974 11597 1316
Size: 4074 4584 1623 4804
Fragments arranged by size:
4804 4584 4074 1623
XbaI T'CTAG_A
Cuts at: 7564 12421 7564
Size: 4857 10228
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2804 6416 7255 10446 11176 11681 2804
Size: 3612 839 3191 730 505 6208
Fragments arranged by size:
6208 3612 3191 839 730 505
XhoI C'TCGA_G
Cuts at: 1943 5874 11979 1943
Size: 3931 6105 5049
Fragments arranged by size:
6105 5049 3931
XmaI C'CCGG_G
Cuts at: 1870 12410 1870
Size: 10540 4545
XmnI GAAnn'nnTTC
Cuts at: 3000 3249 4905 8593 8953 10414 10673 13214 3000
Size: 249 1656 3688 360 1461 259 2541 4871
Fragments arranged by size:
4871 3688 2541 1656 1461 360 259 249
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 12893 276
Size: 53 83 186 936 11359 2468
Fragments arranged by size:
11359 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI
PstI PvuI PvuII SacI SacII SalI SbfI ScaI
SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbeI BsiWI BsmBI BstZ17I
EcoRV FseI FspAI KasI NarI NruI PacI RsrII
SanDI SapI SfiI SfoI SrfI SwaI