Restriction map for
pFN21AB4535
(Circular) (Six-base) MAPSORT of: pFN21AB4535.seq Check: 1860 from: 1 to: 6043
pFN21AB4535 6043 bp
With 182 enzymes: SgfI *
February 27, 2009 14:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2429 2863 2429
Size: 434 5609
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3853 278
Size: 53 83 186 936 2317 2468
Fragments arranged by size:
2468 2317 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3386 1937
Size: 1449 4594
Acc65I G'GTAC_C
Cuts at: 3364 3364
Size: 6043
AclI AA'CG_TT
Cuts at: 1563 4170 4543 1563
Size: 2607 373 3063
Fragments arranged by size:
3063 2607 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2510 3080 3226 4106 5490 5758 1632
Size: 878 570 146 880 1384 268 1917
Fragments arranged by size:
1917 1384 880 878 570 268 146
AfeI AGC'GCT
Cuts at: 2556 2945 2556
Size: 389 5654
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5846
AflIII A'CryG_T
Cuts at: 1203 2205 4942 5919 1203
Size: 1002 2737 977 1327
Fragments arranged by size:
2737 1327 1002 977
AgeI A'CCGG_T
Cuts at: 4874 4874
Size: 6043
AhdI GACnn_n'nnGTC
Cuts at: 4772 4772
Size: 6043
AleI CACnn'nnGTG
Cuts at: 1166 2924 3285 1166
Size: 1758 361 3924
Fragments arranged by size:
3924 1758 361
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2159 1184
Size: 975 5068
AlwNI CAG_nnn'CTG
Cuts at: 2332 3786 5358 2332
Size: 1454 1572 3017
Fragments arranged by size:
3017 1572 1454
ApaLI G'TGCA_C
Cuts at: 4100 5256 4100
Size: 1156 4887
ApoI r'AATT_y
Cuts at: 1493 3352 3578 3730 5839 1493
Size: 1859 226 152 2109 1697
Fragments arranged by size:
2109 1859 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4597 160
Size: 872 3565 1606
Fragments arranged by size:
3565 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6043
AvaI C'yCGr_G
Cuts at: 1870 1943 2126 2655 3182 3368 1870
Size: 73 183 529 527 186 4545
Fragments arranged by size:
4545 529 527 186 183 73
BamHI G'GATC_C
Cuts at: 3373 3740 3373
Size: 367 5676
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2023 2079 2190 2427 3253
Size: 325 217 627 236 56 111 237 826
Cuts at: 3253 3364 4819 618
Size: 111 1455 1842
Fragments arranged by size:
1842 1455 826 627 325 237 236 217
111 111 56
BanII G_rGCy'C
Cuts at: 1391 3192 3210 3362 1391
Size: 1801 18 152 4072
Fragments arranged by size:
4072 1801 152 18
BbeI G_GCGC'C
Cuts at: 2083 2194 2431 2083
Size: 111 237 5695
Fragments arranged by size:
5695 237 111
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5072
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2389 2423 4234 4268 1903
Size: 34 452 34 1811 34 3678
Fragments arranged by size:
3678 1811 452 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3935 5145 3935
Size: 1210 4833
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6043
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2678 4654 136
Size: 107 122 71 631 605 1006 1976 1525
Fragments arranged by size:
1976 1525 1006 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6038 6038
Size: 6043
BlpI GC'TnA_GC
Cuts at: 3460 3460
Size: 6043
Bme1580I G_kGCm'C
Cuts at: 1790 2028 2319 3258 3339 4104 5260 1790
Size: 238 291 939 81 765 1156 2573
Fragments arranged by size:
2573 1156 939 765 291 238 81
BmgBI CAC'GTC
Cuts at: 1318 2206 2863 1318
Size: 888 657 4498
Fragments arranged by size:
4498 888 657
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3774 4732 442
Size: 416 53 83 399 24 2357 958 1753
Fragments arranged by size:
2357 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6043
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4703 1239
Size: 3464 2579
Bpu10I CC'TnA_GC
Cuts at: 2478 2478
Size: 6043
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4168 5033 5331 4168
Size: 865 298 4880
Fragments arranged by size:
4880 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2384 2652 4706 882
Size: 617 885 268 2054 2219
Fragments arranged by size:
2219 2054 885 617 268
BsaAI yAC'GTr
Cuts at: 493 1204 3834 493
Size: 711 2630 2702
Fragments arranged by size:
2702 2630 711
BsaBI GATnn'nnATC
Cuts at: 6037 6037
Size: 6043
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2080 2191 2231 2359
Size: 53 83 186 936 547 111 40 128
Cuts at: 2359 2428 3850 4232 4867 275
Size: 69 1422 382 635 1451
Fragments arranged by size:
1451 1422 936 635 547 382 186 128
111 83 69 53 40
BsaWI w'CCGG_w
Cuts at: 2824 4476 4874 5148 5295 5706 2824
Size: 1652 398 274 147 411 3161
Fragments arranged by size:
3161 1652 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5818
Fragments arranged by size:
5818 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2510 1186
Size: 1324 4719
BseYI C'CCAG_C
Cuts at: 2088 2131 2551 2598 2641 2646 3275 5246 5742
Size: 43 420 47 43 5 629 1971 496
Cuts at: 5742 2088
Size: 2389
Fragments arranged by size:
2389 1971 629 496 420 47 43 43
5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2079 2852 2960 1163
Size: 573 343 773 108 4246
Fragments arranged by size:
4246 773 573 343 108
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2262 2305 3493 4254 5282 1540
Size: 275 186 261 43 1188 761 1028 2301
Fragments arranged by size:
2301 1188 1028 761 275 261 186 43
BsiHKAI G_wGCw'C
Cuts at: 2247 2625 3210 4104 4189 5260 2247
Size: 378 585 894 85 1071 3030
Fragments arranged by size:
3030 1071 894 585 378 85
BsmI GAATG_Cn'
Cuts at: 2530 3554 3647 2530
Size: 1024 93 4926
Fragments arranged by size:
4926 1024 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2028 2247 2319 2625 3192 3210 3258
Size: 399 238 219 72 306 567 18 48
Cuts at: 3258 3339 3362 4104 4189 5260 1391
Size: 81 23 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 567 399 306 238 219
85 81 72 48 23 18
BspEI T'CCGG_A
Cuts at: 2824 5706 2824
Size: 2882 3161
BspHI T'CATG_A
Cuts at: 2811 2932 3932 5662 2811
Size: 121 1000 1730 3192
Fragments arranged by size:
3192 1730 1000 121
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2148 2429 2863 3398 844
Size: 1304 281 434 535 3489
Fragments arranged by size:
3489 1304 535 434 281
BsrBI CCG'CTC
Cuts at: 1394 1823 3930 1394
Size: 429 2107 3507
Fragments arranged by size:
3507 2107 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4538 4712 65
Size: 1183 3290 174 1396
Fragments arranged by size:
3290 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2076 2233 4687 4874 1700
Size: 110 266 157 2454 187 2869
Fragments arranged by size:
2869 2454 266 187 157 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6043
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6043
BssSI C'ACGA_G
Cuts at: 4103 5115 4103
Size: 1012 5031
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6043
BstBI TT'CG_AA
Cuts at: 4847 4847
Size: 6043
BstEII G'GTnAC_C
Cuts at: 1184 2278 2773 2894 3304 1184
Size: 1094 495 121 410 3923
Fragments arranged by size:
3923 1094 495 410 121
BstXI CCAn_nnnn'nTGG
Cuts at: 2041 2041
Size: 6043
BstYI r'GATC_y
Cuts at: 1972 2328 3373 3740 4125 4142 5594 5680 5692
Size: 356 1045 367 385 17 1452 86 12
Cuts at: 5692 6038 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1045 385 367 356 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2459 3021 2459
Size: 562 5481
BtgI C'CryG_G
Cuts at: 513 1065 2004 2284 3283 513
Size: 552 939 280 999 3273
Fragments arranged by size:
3273 999 939 552 280
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5304
Fragments arranged by size:
5304 683 56
BtsI GCAGTG_nn'
Cuts at: 2120 2919 3559 4353 4373 2120
Size: 799 640 794 20 3790
Fragments arranged by size:
3790 799 794 640 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6043
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6008
DraI TTT'AAA
Cuts at: 3347 3347
Size: 6043
DraIII CAC_nnn'GTG
Cuts at: 3133 3133
Size: 6043
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5050 809
Size: 507 3734 1802
Fragments arranged by size:
3734 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2031 2043 2259
Size: 54 1425 50 275 18 201 12 216
Cuts at: 2259 3490 4379 6008 8
Size: 1231 889 1629 43
Fragments arranged by size:
1629 1425 1231 889 275 216 201 54
50 43 18 12
EagI C'GGCC_G
Cuts at: 1537 1812 2259 3490 1537
Size: 275 447 1231 4090
Fragments arranged by size:
4090 1231 447 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3215 3221 3973 1353
Size: 1862 6 752 3423
Fragments arranged by size:
3423 1862 752 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4630 5004 5150 387
Size: 1064 138 3041 374 146 1280
Fragments arranged by size:
3041 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3208 3208
Size: 6043
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2510 3080 3226 4106 4703 5490 5758
Size: 393 878 570 146 880 597 787 268
Cuts at: 5758 1239
Size: 1524
Fragments arranged by size:
1524 880 878 787 597 570 393 268
146
EcoNI CCTnn'n_nnAGG
Cuts at: 2477 2666 2477
Size: 189 5854
EcoO109I rG'GnC_Cy
Cuts at: 2111 2569 2638 2800 2111
Size: 458 69 162 5354
Fragments arranged by size:
5354 458 162 69
EcoRI G'AATT_C
Cuts at: 3352 3730 3352
Size: 378 5665
FspI TGC'GCA
Cuts at: 4549 4549
Size: 6043
HaeII r_GCGC'y
Cuts at: 1678 2083 2194 2276 2431 2558 2839 2947 5190
Size: 405 111 82 155 127 281 108 2243
Cuts at: 5190 1678
Size: 2531
Fragments arranged by size:
2531 2243 405 281 155 127 111 108
82
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2744 2776 2806 2838 4684
Size: 32 135 32 1172 32 30 32 1846
Cuts at: 4684 4716 4758 4790 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1846 1172 135 42 32 32 32
32 32 32 30
HincII GTy'rAC
Cuts at: 669 1657 1938 3387 3633 669
Size: 988 281 1449 246 3079
Fragments arranged by size:
3079 1449 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6043
HpaI GTT'AAC
Cuts at: 3633 3633
Size: 6043
KasI G'GCGC_C
Cuts at: 2079 2190 2427 2079
Size: 111 237 5695
Fragments arranged by size:
5695 237 111
KpnI G_GTAC'C
Cuts at: 3368 3368
Size: 6043
MfeI C'AATT_G
Cuts at: 3642 3642
Size: 6043
MluI A'CGCG_T
Cuts at: 5919 5919
Size: 6043
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2869 5157 5341 1305
Size: 1564 2288 184 2007
Fragments arranged by size:
2288 2007 1564 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2033 2045 10
Size: 54 1425 343 201 12 4008
Fragments arranged by size:
4008 1425 343 201 54 12
MslI CAynn'nnrTG
Cuts at: 518 1166 2039 2744 2826 2924 3285 4001 4360
Size: 648 873 705 82 98 361 716 359
Cuts at: 4360 4519 518
Size: 159 2042
Fragments arranged by size:
2042 873 716 705 648 361 359 159
98 82
MspA1I CmG'CkG
Cuts at: 1606 2221 2602 3459 4136 5284 5529 1606
Size: 615 381 857 677 1148 245 2120
Fragments arranged by size:
2120 1148 857 677 615 381 245
NarI GG'CG_CC
Cuts at: 2080 2191 2428 2080
Size: 111 237 5695
Fragments arranged by size:
5695 237 111
NcoI C'CATG_G
Cuts at: 513 1065 2004 3283 513
Size: 552 939 1279 3273
Fragments arranged by size:
3273 1279 939 552
NdeI CA'TA_TG
Cuts at: 387 2979 387
Size: 2592 3451
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6043
NotI GC'GGCC_GC
Cuts at: 3490 3490
Size: 6043
NspI r_CATG'y
Cuts at: 1132 1229 2150 2911 3180 3401 4946 1132
Size: 97 921 761 269 221 1545 2229
Fragments arranged by size:
2229 1545 921 761 269 221 97
PasI CC'CwG_GG
Cuts at: 1348 2965 3121 1348
Size: 1617 156 4270
Fragments arranged by size:
4270 1617 156
PciI A'CATG_T
Cuts at: 4942 4942
Size: 6043
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2332 1108
Size: 1224 4819
PfoI T'CCnGG_A
Cuts at: 3111 3111
Size: 6043
PmeI GTTT'AAAC
Cuts at: 3347 3347
Size: 6043
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4112 4144 5625 5657 4112
Size: 32 1481 32 4498
Fragments arranged by size:
4498 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2569 2638 2569
Size: 69 5974
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6043
PsiI TTA'TAA
Cuts at: 3613 3613
Size: 6043
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2153 2185 2153
Size: 32 6011
PstI C_TGCA'G
Cuts at: 830 2220 3237 3395 830
Size: 1390 1017 158 3478
Fragments arranged by size:
3478 1390 1017 158
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6043
PvuII CAG'CTG
Cuts at: 2221 2602 2221
Size: 381 5662
SacI G_AGCT'C
Cuts at: 3210 3210
Size: 6043
SalI G'TCGA_C
Cuts at: 1936 3385 1936
Size: 1449 4594
SanDI GG'GwC_CC
Cuts at: 2638 2638
Size: 6043
SapI GCTCTTCn'nnn_
Cuts at: 3215 3215
Size: 6043
SbfI CC_TGCA'GG
Cuts at: 3395 3395
Size: 6043
ScaI AGT'ACT
Cuts at: 4291 6003 4291
Size: 1712 4331
SfcI C'TryA_G
Cuts at: 826 1045 2216 3233 3391 4526 5207 5398 826
Size: 219 1171 1017 158 1135 681 191 1471
Fragments arranged by size:
1471 1171 1135 1017 681 219 191 158
SfoI GGC'GCC
Cuts at: 2081 2192 2429 2081
Size: 111 237 5695
Fragments arranged by size:
5695 237 111
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6043
SgrAI Cr'CCGG_yG
Cuts at: 1700 2076 2233 1700
Size: 376 157 5510
Fragments arranged by size:
5510 376 157
SmaI CCC'GGG
Cuts at: 1872 2128 3370 1872
Size: 256 1242 4545
Fragments arranged by size:
4545 1242 256
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4147 5048 5310 820
Size: 197 926 2204 901 262 1553
Fragments arranged by size:
2204 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3834 493
Size: 3341 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6043
SphI G_CATG'C
Cuts at: 1132 3180 3401 1132
Size: 2048 221 3774
Fragments arranged by size:
3774 2048 221
SrfI GCCC'GGGC
Cuts at: 2128 2128
Size: 6043
SspI AAT'ATT
Cuts at: 5 52 3967 5
Size: 47 3915 2081
Fragments arranged by size:
3915 2081 47
StuI AGG'CCT
Cuts at: 1517 2672 3146 1517
Size: 1155 474 4414
Fragments arranged by size:
4414 1155 474
StyI C'CwwG_G
Cuts at: 513 1065 2004 2353 2697 3283 3482 513
Size: 552 939 349 344 586 199 3074
Fragments arranged by size:
3074 939 586 552 349 344 199
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2339 2882 2957 4070 4087 4240 4425
Size: 1255 759 543 75 1113 17 153 185
Cuts at: 4425 4870 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1113 759 543 445 185 153
75 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3001 4289 6001
Size: 274 80 33 51 1443 1024 1288 1712
Cuts at: 6001 96
Size: 138
Fragments arranged by size:
1712 1443 1288 1024 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6003 6035 6003
Size: 32 6011
Tth111I GACn'n_nGTC
Cuts at: 1316 2957 1316
Size: 1641 4402
XbaI T'CTAG_A
Cuts at: 3379 3379
Size: 6043
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2380 2380
Size: 6043
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6043
XmaI C'CCGG_G
Cuts at: 1870 2126 3368 1870
Size: 256 1242 4545
Fragments arranged by size:
4545 1242 256
XmnI GAAnn'nnTTC
Cuts at: 3086 4172 3086
Size: 1086 4957
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3851 276
Size: 53 83 186 936 2317 2468
Fragments arranged by size:
2468 2317 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NarI NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PsrI
PstI PvuI PvuII SacI SalI SanDI SapI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI
BsiWI BsmBI BstZ17I EcoRV FalI FseI FspAI NaeI
NgoMIV NruI NsiI PacI PmlI PspOMI PspXI RsrII
SacII SexAI SfiI SwaI