Restriction map for
pFN21AB4524
(Circular) (Six-base) MAPSORT of: pFN21AB4524.seq Check: 3991 from: 1 to: 8521
pFN21AB4524 8521 bp
With 182 enzymes: SgfI *
July 27, 2009 13:41 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3811 3811
Size: 8521
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6331 278
Size: 53 83 186 936 4795 2468
Fragments arranged by size:
4795 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5864 1937
Size: 3927 4594
Acc65I G'GTAC_C
Cuts at: 5842 5842
Size: 8521
AclI AA'CG_TT
Cuts at: 1563 2884 6648 7021 1563
Size: 1321 3764 373 3063
Fragments arranged by size:
3764 3063 1321 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2147 2580 2954 3152 3456 3536 4754 6584
Size: 515 433 374 198 304 80 1218 1830
Cuts at: 6584 7968 8236 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1830 1384 1218 515 433 374 304
268 198 80
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8324
AflIII A'CryG_T
Cuts at: 1203 7420 8397 1203
Size: 6217 977 1327
Fragments arranged by size:
6217 1327 977
AgeI A'CCGG_T
Cuts at: 7352 7352
Size: 8521
AhdI GACnn_n'nnGTC
Cuts at: 5035 7250 5035
Size: 2215 6306
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 8521
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3609 1184
Size: 2425 6096
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2976 3008 4257 4289 2976
Size: 32 1249 32 7208
Fragments arranged by size:
7208 1249 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2302 2896 3613 6264 7836 2302
Size: 594 717 2651 1572 2987
Fragments arranged by size:
2987 2651 1572 717 594
ApaI G_GGCC'C
Cuts at: 2034 5658 2034
Size: 3624 4897
ApaLI G'TGCA_C
Cuts at: 6578 7734 6578
Size: 1156 7365
ApoI r'AATT_y
Cuts at: 1493 2117 2212 4101 5199 5227 5402 5830 6056
Size: 624 95 1889 1098 28 175 428 226
Cuts at: 6056 6208 8317 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1889 1697 1098 624 428 226 175
152 95 28
AseI AT'TA_AT
Cuts at: 160 1032 3395 7075 160
Size: 872 2363 3680 1606
Fragments arranged by size:
3680 2363 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8521
AvaI C'yCGr_G
Cuts at: 1870 1943 2046 5846 1870
Size: 73 103 3800 4545
Fragments arranged by size:
4545 3800 103 73
BamHI G'GATC_C
Cuts at: 2749 5851 6218 2749
Size: 3102 367 5052
Fragments arranged by size:
5052 3102 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3369 5842 7297 618
Size: 325 217 627 1582 2473 1455 1842
Fragments arranged by size:
2473 1842 1582 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2034 2072 2429 5658 5840 1391
Size: 643 38 357 3229 182 4072
Fragments arranged by size:
4072 3229 643 357 182 38
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 5600 928
Size: 971 3701 3849
Fragments arranged by size:
3849 3701 971
BbvCI CC'TCA_GC
Cuts at: 4371 4371
Size: 8521
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3035 3069 6712 6746 1903
Size: 34 1098 34 3643 34 3678
Fragments arranged by size:
3678 3643 1098 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6413 7623 6413
Size: 1210 7311
BclI T'GATC_A
Cuts at: 1548 2955 3400 1548
Size: 1407 445 6669
Fragments arranged by size:
6669 1407 445
BfrBI ATG'CAT
Cuts at: 5326 5326
Size: 8521
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 7132 136
Size: 107 122 71 631 605 5460 1525
Fragments arranged by size:
5460 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3547 8516 3547
Size: 4969 3552
BlpI GC'TnA_GC
Cuts at: 2489 2784 5938 2489
Size: 295 3154 5072
Fragments arranged by size:
5072 3154 295
Bme1580I G_kGCm'C
Cuts at: 1790 2034 4790 4797 5658 6582 7738 1790
Size: 244 2756 7 861 924 1156 2573
Fragments arranged by size:
2756 2573 1156 924 861 244 7
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8521
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4280 6252 7210
Size: 416 53 83 399 24 2863 1972 958
Cuts at: 7210 442
Size: 1753
Fragments arranged by size:
2863 1972 1753 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 5705 1056
Size: 4649 3872
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2101 4396 5576 2101
Size: 2295 1180 5046
Fragments arranged by size:
5046 2295 1180
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2171 2519 3359 3927 4035 4149 4661 4960
Size: 932 348 840 568 108 114 512 299
Cuts at: 4960 7181 1239
Size: 2221 2579
Fragments arranged by size:
2579 2221 932 840 568 512 348 299
114 108
Bpu10I CC'TnA_GC
Cuts at: 4371 4371
Size: 8521
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5093 5807 6646 7511 7809 5093
Size: 714 839 865 298 5805
Fragments arranged by size:
5805 865 839 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5333 7184 882
Size: 617 3834 1851 2219
Fragments arranged by size:
3834 2219 1851 617
BsaAI yAC'GTr
Cuts at: 493 1204 4907 6312 493
Size: 711 3703 1405 2702
Fragments arranged by size:
3703 2702 1405 711
BsaBI GATnn'nnATC
Cuts at: 8515 8515
Size: 8521
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 6328 6710 7345 275
Size: 53 83 186 936 4795 382 635 1451
Fragments arranged by size:
4795 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4341 6954 7352 7626 7773 8184 4341
Size: 2613 398 274 147 411 4678
Fragments arranged by size:
4678 2613 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2363 2393 2447 2477 3706
Size: 30 165 30 960 30 54 30 1229
Cuts at: 3706 3736 3811 3814 3841 3844 3943 3973 1178
Size: 30 75 3 27 3 99 30 5726
Fragments arranged by size:
5726 1229 960 165 99 75 54 30
30 30 30 30 30 27 3 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2339 3816 3819 3822 3825 3828 3831 3834
Size: 1153 1477 3 3 3 3 3 3
Cuts at: 3834 3837 3840 3843 3877 4029 4107 4140 4143
Size: 3 3 3 34 152 78 33 3
Cuts at: 4143 4182 4913 1186
Size: 39 731 4794
Fragments arranged by size:
4794 1477 1153 731 152 78 39 34
33 3 3 3 3 3 3 3
3 3 3
BseYI C'CCAG_C
Cuts at: 2621 3630 5233 5356 7724 8220 2621
Size: 1009 1603 123 2368 496 2922
Fragments arranged by size:
2922 2368 1603 1009 496 123
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3317 4355 5706 1163
Size: 573 1581 1038 1351 3978
Fragments arranged by size:
3978 1581 1351 1038 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2038 5971 6732 7760 1540
Size: 275 186 37 3933 761 1028 2301
Fragments arranged by size:
3933 2301 1028 761 275 186 37
BsiHKAI G_wGCw'C
Cuts at: 2429 3745 6582 6667 7738 2429
Size: 1316 2837 85 1071 3212
Fragments arranged by size:
3212 2837 1316 1071 85
BsmI GAATG_Cn'
Cuts at: 3574 4146 4215 4972 5326 5578 5636 6032 6125
Size: 572 69 757 354 252 58 396 93
Cuts at: 6125 3574
Size: 5970
Fragments arranged by size:
5970 757 572 396 354 252 93 69
58
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2034 2072 2429 3745 4790 4797 5658
Size: 399 244 38 357 1316 1045 7 861
Cuts at: 5658 5840 6582 6667 7738 1391
Size: 182 742 85 1071 2174
Fragments arranged by size:
2174 1316 1071 1045 861 742 399 357
244 182 85 38 7
BspEI T'CCGG_A
Cuts at: 4341 8184 4341
Size: 3843 4678
BspHI T'CATG_A
Cuts at: 2599 6410 8140 2599
Size: 3811 1730 2980
Fragments arranged by size:
3811 2980 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3811 5876 844
Size: 2967 2065 3489
Fragments arranged by size:
3489 2967 2065
BsrBI CCG'CTC
Cuts at: 1394 1823 5530 6408 1394
Size: 429 3707 878 3507
Fragments arranged by size:
3707 3507 878 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2252 2856 7016 7190 65
Size: 1183 1004 604 4160 174 1396
Fragments arranged by size:
4160 1396 1183 1004 604 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3366 7165 7352 1700
Size: 110 1556 3799 187 2869
Fragments arranged by size:
3799 2869 1556 187 110
BsrGI T'GTAC_A
Cuts at: 96 2391 96
Size: 2295 6226
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8521
BssSI C'ACGA_G
Cuts at: 6581 7593 6581
Size: 1012 7509
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 8521
BstBI TT'CG_AA
Cuts at: 7325 7325
Size: 8521
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8521
BstXI CCAn_nnnn'nTGG
Cuts at: 4366 5803 4366
Size: 1437 7084
BstYI r'GATC_y
Cuts at: 1972 2749 3547 5413 5851 6218 6603 6620 8072
Size: 777 798 1866 438 367 385 17 1452
Cuts at: 8072 8158 8170 8516 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1866 1452 798 777 438 385 367
346 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3978 3978
Size: 8521
BtgI C'CryG_G
Cuts at: 513 1065 2004 2038 2745 5730 513
Size: 552 939 34 707 2985 3304
Fragments arranged by size:
3304 2985 939 707 552 34
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 5598 487
Size: 683 56 4372 3410
Fragments arranged by size:
4372 3410 683 56
BtsI GCAGTG_nn'
Cuts at: 3569 4884 6037 6831 6851 3569
Size: 1315 1153 794 20 5239
Fragments arranged by size:
5239 1315 1153 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8521
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3846 3881 4065 4100 5767 5802 529
Size: 35 3282 35 184 35 1667 35 3248
Fragments arranged by size:
3282 3248 1667 184 35 35 35 35
DraI TTT'AAA
Cuts at: 5825 5825
Size: 8521
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7528 809
Size: 507 6212 1802
Fragments arranged by size:
6212 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2035 5809 5968
Size: 54 1425 50 275 18 205 3774 159
Cuts at: 5968 6857 8486 8
Size: 889 1629 43
Fragments arranged by size:
3774 1629 1425 889 275 205 159 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2035 5968 1537
Size: 275 223 3933 4090
Fragments arranged by size:
4090 3933 275 223
EarI CTCTTCn'nnn_
Cuts at: 1353 2607 3108 4009 4167 4832 5543 5642 6451
Size: 1254 501 901 158 665 711 99 809
Cuts at: 6451 1353
Size: 3423
Fragments arranged by size:
3423 1254 901 809 711 665 501 158
99
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 7108 7482 7628 387
Size: 1064 138 5519 374 146 1280
Fragments arranged by size:
5519 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2427 2427
Size: 8521
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2147 2171 2519 2580 2954 3152 3359
Size: 393 515 24 348 61 374 198 207
Cuts at: 3359 3456 3536 3927 4035 4149 4661 4754 4960
Size: 97 80 391 108 114 512 93 206
Cuts at: 4960 6584 7181 7968 8236 1239
Size: 1624 597 787 268 1524
Fragments arranged by size:
1624 1524 787 597 515 512 393 391
374 348 268 207 206 198 114 108
97 93 80 61 24
EcoNI CCTnn'n_nnAGG
Cuts at: 2321 4382 5348 2321
Size: 2061 966 5494
Fragments arranged by size:
5494 2061 966
EcoO109I rG'GnC_Cy
Cuts at: 2030 2043 3049 3610 4208 4375 5654 5655 2030
Size: 13 1006 561 598 167 1279 1 4896
Fragments arranged by size:
4896 1279 1006 598 561 167 13 1
EcoRI G'AATT_C
Cuts at: 4101 5830 6208 4101
Size: 1729 378 6414
Fragments arranged by size:
6414 1729 378
FspI TGC'GCA
Cuts at: 7027 7027
Size: 8521
HaeII r_GCGC'y
Cuts at: 1678 7668 1678
Size: 5990 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2363 2395 2447 2479 2947
Size: 32 135 32 791 32 52 32 468
Cuts at: 2947 2979 2988 3020 4862 4894 5021 5053 5590
Size: 32 9 32 1842 32 127 32 537
Cuts at: 5590 5622 7162 7194 7236 7268 1373
Size: 32 1540 32 42 32 2626
Fragments arranged by size:
2626 1842 1540 791 537 468 135 127
52 42 32 32 32 32 32 32
32 32 32 32 32 9
HincII GTy'rAC
Cuts at: 669 1657 1938 3480 5865 6111 669
Size: 988 281 1542 2385 246 3079
Fragments arranged by size:
3079 2385 1542 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2670 3539 4316 4361 5021 748
Size: 1922 869 777 45 660 4248
Fragments arranged by size:
4248 1922 869 777 660 45
HpaI GTT'AAC
Cuts at: 3480 6111 3480
Size: 2631 5890
KpnI G_GTAC'C
Cuts at: 5846 5846
Size: 8521
MfeI C'AATT_G
Cuts at: 6120 6120
Size: 8521
MluI A'CGCG_T
Cuts at: 8397 8397
Size: 8521
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2688 2920 3447 4539 4933 5665 7635 7819
Size: 1383 232 527 1092 394 732 1970 184
Cuts at: 7819 1305
Size: 2007
Fragments arranged by size:
2007 1970 1383 1092 732 527 394 232
184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 6699
Fragments arranged by size:
6699 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3155 3161 5567 6479 6838 6997 518
Size: 648 1989 6 2406 912 359 159 2042
Fragments arranged by size:
2406 2042 1989 912 648 359 159 6
MspA1I CmG'CkG
Cuts at: 1606 2040 2493 2896 2953 2978 5237 5937 6614
Size: 434 453 403 57 25 2259 700 677
Cuts at: 6614 7762 8007 1606
Size: 1148 245 2120
Fragments arranged by size:
2259 2120 1148 700 677 453 434 403
245 57 25
NaeI GCC'GGC
Cuts at: 3368 3368
Size: 8521
NcoI C'CATG_G
Cuts at: 513 1065 2004 2745 5730 513
Size: 552 939 741 2985 3304
Fragments arranged by size:
3304 2985 939 741 552
NdeI CA'TA_TG
Cuts at: 387 2227 387
Size: 1840 6681
NgoMIV G'CCGG_C
Cuts at: 3366 3366
Size: 8521
NheI G'CTAG_C
Cuts at: 1052 5701 1052
Size: 4649 3872
NotI GC'GGCC_GC
Cuts at: 5968 5968
Size: 8521
NsiI A_TGCA'T
Cuts at: 5328 5328
Size: 8521
NspI r_CATG'y
Cuts at: 1132 1229 4612 4896 5879 7424 1132
Size: 97 3383 284 983 1545 2229
Fragments arranged by size:
3383 2229 1545 983 284 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 8521
PciI A'CATG_T
Cuts at: 7420 7420
Size: 8521
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 8521
PfoI T'CCnGG_A
Cuts at: 4174 4201 4638 4174
Size: 27 437 8057
Fragments arranged by size:
8057 437 27
PmeI GTTT'AAAC
Cuts at: 5825 5825
Size: 8521
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6590 6622 8103 8135 6590
Size: 32 1481 32 6976
Fragments arranged by size:
6976 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2043 3049 3610 4375 2043
Size: 1006 561 765 6189
Fragments arranged by size:
6189 1006 765 561
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8521
PsiI TTA'TAA
Cuts at: 4323 6091 4323
Size: 1768 6753
PspOMI G'GGCC_C
Cuts at: 2030 5654 2030
Size: 3624 4897
PstI C_TGCA'G
Cuts at: 830 3249 3451 4282 5625 5873 830
Size: 2419 202 831 1343 248 3478
Fragments arranged by size:
3478 2419 1343 831 248 202
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8521
PvuII CAG'CTG
Cuts at: 2493 2953 5237 2493
Size: 460 2284 5777
Fragments arranged by size:
5777 2284 460
SacI G_AGCT'C
Cuts at: 2429 2429
Size: 8521
SacII CC_GC'GG
Cuts at: 2041 2041
Size: 8521
SalI G'TCGA_C
Cuts at: 1936 5863 1936
Size: 3927 4594
SanDI GG'GwC_CC
Cuts at: 2043 2043
Size: 8521
SbfI CC_TGCA'GG
Cuts at: 5873 5873
Size: 8521
ScaI AGT'ACT
Cuts at: 3293 5366 6769 8481 3293
Size: 2073 1403 1712 3333
Fragments arranged by size:
3333 2073 1712 1403
SexAI A'CCwGG_T
Cuts at: 3769 3769
Size: 8521
SfcI C'TryA_G
Cuts at: 826 1045 2948 3245 3447 3497 4278 5621 5869
Size: 219 1903 297 202 50 781 1343 248
Cuts at: 5869 7004 7685 7876 826
Size: 1135 681 191 1471
Fragments arranged by size:
1903 1471 1343 1135 781 681 297 248
219 202 191 50
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8521
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8521
SmaI CCC'GGG
Cuts at: 1872 2048 5848 1872
Size: 176 3800 4545
Fragments arranged by size:
4545 3800 176
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5108 5786 6625 7526 7788 820
Size: 197 926 3165 678 839 901 262 1553
Fragments arranged by size:
3165 1553 926 901 839 678 262 197
SnaBI TAC'GTA
Cuts at: 493 4907 6312 493
Size: 4414 1405 2702
Fragments arranged by size:
4414 2702 1405
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8521
SphI G_CATG'C
Cuts at: 1132 4612 5879 1132
Size: 3480 1267 3774
Fragments arranged by size:
3774 3480 1267
SspI AAT'ATT
Cuts at: 5 52 6445 5
Size: 47 6393 2081
Fragments arranged by size:
6393 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 8521
StyI C'CwwG_G
Cuts at: 513 1065 2004 2400 2745 5508 5718 5730 5748
Size: 552 939 396 345 2763 210 12 18
Cuts at: 5748 5796 5960 513
Size: 48 164 3074
Fragments arranged by size:
3074 2763 939 552 396 345 210 164
48 18 12
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2530 6548 6565 6718 6903 7348 325
Size: 1255 950 4018 17 153 185 445 1498
Fragments arranged by size:
4018 1498 1255 950 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2391 3291 5364
Size: 274 80 33 51 1443 414 900 2073
Cuts at: 5364 6767 8479 96
Size: 1403 1712 138
Fragments arranged by size:
2073 1712 1443 1403 900 414 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3221 3253 3775 3807 3814 3846 8481 8513 3221
Size: 32 522 32 7 32 4635 32 3229
Fragments arranged by size:
4635 3229 522 32 32 32 32 7
Tth111I GACn'n_nGTC
Cuts at: 1316 4666 1316
Size: 3350 5171
XbaI T'CTAG_A
Cuts at: 5611 5857 5611
Size: 246 8275
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3685 3685
Size: 8521
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8521
XmaI C'CCGG_G
Cuts at: 1870 2046 5846 1870
Size: 176 3800 4545
Fragments arranged by size:
4545 3800 176
XmnI GAAnn'nnTTC
Cuts at: 2215 3183 6650 2215
Size: 968 3467 4086
Fragments arranged by size:
4086 3467 968
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6329 276
Size: 53 83 186 936 4795 2468
Fragments arranged by size:
4795 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII
SalI SanDI SbfI ScaI SexAI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbeI BsiWI BsmBI BstZ17I
DraIII EcoRV FalI FseI FspAI KasI NarI NruI
PacI PmlI PspXI PsrI RsrII SapI SfiI SfoI
SrfI SwaI