Restriction map for
pFN21AB4523
(Circular) (Six-base) MAPSORT of: pFN21AB4523.seq Check: 4672 from: 1 to: 6220
pFN21AB4523 6220 bp
With 182 enzymes: SgfI *
February 27, 2009 14:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2216 2964 2216
Size: 748 5472
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4030 278
Size: 53 83 186 936 2494 2468
Fragments arranged by size:
2494 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2553 3563 1937
Size: 616 1010 4594
Fragments arranged by size:
4594 1010 616
Acc65I G'GTAC_C
Cuts at: 3541 3541
Size: 6220
AclI AA'CG_TT
Cuts at: 1563 4347 4720 1563
Size: 2784 373 3063
Fragments arranged by size:
3063 2784 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3174 4283 5667 5935 1632
Size: 1542 1109 1384 268 1917
Fragments arranged by size:
1917 1542 1384 1109 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6023
AflIII A'CryG_T
Cuts at: 1203 2136 2138 5119 6096 1203
Size: 933 2 2981 977 1327
Fragments arranged by size:
2981 1327 977 933 2
AgeI A'CCGG_T
Cuts at: 2889 5051 2889
Size: 2162 4058
AhdI GACnn_n'nnGTC
Cuts at: 3013 4949 3013
Size: 1936 4284
AleI CACnn'nnGTG
Cuts at: 1166 2836 1166
Size: 1670 4550
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6220
AlwNI CAG_nnn'CTG
Cuts at: 2098 2488 2576 3095 3358 3963 5535 2098
Size: 390 88 519 263 605 1572 2783
Fragments arranged by size:
2783 1572 605 519 390 263 88
ApaI G_GGCC'C
Cuts at: 2082 2375 2082
Size: 293 5927
ApaLI G'TGCA_C
Cuts at: 2110 4277 5433 2110
Size: 2167 1156 2897
Fragments arranged by size:
2897 2167 1156
ApoI r'AATT_y
Cuts at: 1493 3529 3755 3907 6016 1493
Size: 2036 226 152 2109 1697
Fragments arranged by size:
2109 2036 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3174 4774 160
Size: 872 2142 1600 1606
Fragments arranged by size:
2142 1606 1600 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6220
AvaI C'yCGr_G
Cuts at: 1870 1943 2082 3221 3545 1870
Size: 73 139 1139 324 4545
Fragments arranged by size:
4545 1139 324 139 73
BamHI G'GATC_C
Cuts at: 3550 3917 3550
Size: 367 5853
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2326 2822 2975 3541 4996
Size: 325 217 627 539 496 153 566 1455
Cuts at: 4996 618
Size: 1842
Fragments arranged by size:
1842 1455 627 566 539 496 325 217
153
BanII G_rGCy'C
Cuts at: 1391 2082 2264 2375 3539 1391
Size: 691 182 111 1164 4072
Fragments arranged by size:
4072 1164 691 182 111
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3485 3513 928
Size: 971 1586 28 3635
Fragments arranged by size:
3635 1586 971 28
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2296 2330 4411 4445 1903
Size: 34 359 34 2081 34 3678
Fragments arranged by size:
3678 2081 359 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4112 5322 4112
Size: 1210 5010
BclI T'GATC_A
Cuts at: 1548 3349 1548
Size: 1801 4419
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4831 136
Size: 107 122 71 631 605 3159 1525
Fragments arranged by size:
3159 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6215 6215
Size: 6220
BlpI GC'TnA_GC
Cuts at: 3494 3637 3494
Size: 143 6077
Bme1580I G_kGCm'C
Cuts at: 1790 2082 2114 2331 2375 2978 3309 4281 5437
Size: 292 32 217 44 603 331 972 1156
Cuts at: 5437 1790
Size: 2573
Fragments arranged by size:
2573 1156 972 603 331 292 217 44
32
BmgBI CAC'GTC
Cuts at: 1318 3511 1318
Size: 2193 4027
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2325 3951 4909
Size: 416 53 83 399 24 908 1626 958
Cuts at: 4909 442
Size: 1753
Fragments arranged by size:
1753 1626 958 908 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6220
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2108 2525 2701 3282 4880 1239
Size: 869 417 176 581 1598 2579
Fragments arranged by size:
2579 1598 869 581 417 176
Bpu10I CC'TnA_GC
Cuts at: 2258 2352 2581 2258
Size: 94 229 5897
Fragments arranged by size:
5897 229 94
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2327 2341 4345 5210 5508 2327
Size: 14 2004 865 298 3039
Fragments arranged by size:
3039 2004 865 298 14
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4883 882
Size: 617 3384 2219
Fragments arranged by size:
3384 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2139 4011 493
Size: 711 935 1872 2702
Fragments arranged by size:
2702 1872 935 711
BsaBI GATnn'nnATC
Cuts at: 6214 6214
Size: 6220
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4027 4409 5044 275
Size: 53 83 186 936 2494 382 635 1451
Fragments arranged by size:
2494 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2889 4653 5051 5325 5472 5883 2889
Size: 1764 398 274 147 411 3226
Fragments arranged by size:
3226 1764 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2546 2576 2674 2704 3176
Size: 30 165 30 1143 30 98 30 472
Cuts at: 3176 3206 1178
Size: 30 4192
Fragments arranged by size:
4192 1143 472 165 98 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2017 3279 1186
Size: 831 1262 4127
Fragments arranged by size:
4127 1262 831
BseYI C'CCAG_C
Cuts at: 3489 5423 5919 3489
Size: 1934 496 3790
Fragments arranged by size:
3790 1934 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2942 3521 1163
Size: 573 1206 579 3862
Fragments arranged by size:
3862 1206 579 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2019 3670 4431 5459 1540
Size: 275 186 18 1651 761 1028 2301
Fragments arranged by size:
2301 1651 1028 761 275 186 18
BsiHKAI G_wGCw'C
Cuts at: 2114 2196 2264 2358 2865 4281 4366 5437 2114
Size: 82 68 94 507 1416 85 1071 2897
Fragments arranged by size:
2897 1416 1071 507 94 85 82 68
BsmI GAATG_Cn'
Cuts at: 3731 3824 3731
Size: 93 6127
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2082 2114 2196 2264 2331 2358 2375
Size: 399 292 32 82 68 67 27 17
Cuts at: 2375 2865 2978 3309 3539 4281 4366 5437 1391
Size: 490 113 331 230 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 490 399 331 292 230
113 85 82 68 67 32 27 17
BspEI T'CCGG_A
Cuts at: 5883 5883
Size: 6220
BspHI T'CATG_A
Cuts at: 2857 3082 4109 5839 2857
Size: 225 1027 1730 3238
Fragments arranged by size:
3238 1730 1027 225
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2216 2280 2964 3575 844
Size: 1372 64 684 611 3489
Fragments arranged by size:
3489 1372 684 611 64
BsrBI CCG'CTC
Cuts at: 1394 1823 4107 1394
Size: 429 2284 3507
Fragments arranged by size:
3507 2284 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3108 4715 4889 65
Size: 1183 1860 1607 174 1396
Fragments arranged by size:
1860 1607 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2060 2399 2889 4864 5051 1700
Size: 110 250 339 490 1975 187 2869
Fragments arranged by size:
2869 1975 490 339 250 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6220
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6220
BssSI C'ACGA_G
Cuts at: 2718 4280 5292 2718
Size: 1562 1012 3646
Fragments arranged by size:
3646 1562 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2118 2200 794
Size: 1324 82 4814
Fragments arranged by size:
4814 1324 82
BstBI TT'CG_AA
Cuts at: 5024 5024
Size: 6220
BstEII G'GTnAC_C
Cuts at: 1184 2336 2586 1184
Size: 1152 250 4818
Fragments arranged by size:
4818 1152 250
BstYI r'GATC_y
Cuts at: 1972 2500 2545 3550 3917 4302 4319 5771 5857
Size: 528 45 1005 367 385 17 1452 86
Cuts at: 5857 5869 6215 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1005 528 385 367 346 86
45 17 12
Bsu36I CC'TnA_GG
Cuts at: 2569 2569
Size: 6220
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4729
Fragments arranged by size:
4729 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2380 487
Size: 683 56 1154 4327
Fragments arranged by size:
4327 1154 683 56
BtsI GCAGTG_nn'
Cuts at: 2284 3398 3736 4530 4550 2284
Size: 1114 338 794 20 3954
Fragments arranged by size:
3954 1114 794 338 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6220
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6185
DraI TTT'AAA
Cuts at: 3199 3524 3199
Size: 325 5895
DraIII CAC_nnn'GTG
Cuts at: 2200 2200
Size: 6220
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5227 809
Size: 507 3911 1802
Fragments arranged by size:
3911 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2016 3667 4556
Size: 54 1425 50 275 18 186 1651 889
Cuts at: 4556 6185 8
Size: 1629 43
Fragments arranged by size:
1651 1629 1425 889 275 186 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2016 3667 1537
Size: 275 204 1651 4090
Fragments arranged by size:
4090 1651 275 204
EarI CTCTTCn'nnn_
Cuts at: 1353 2719 4150 1353
Size: 1366 1431 3423
Fragments arranged by size:
3423 1431 1366
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2024 4807 5181 5327 387
Size: 1064 138 435 2783 374 146 1280
Fragments arranged by size:
2783 1280 1064 435 374 146 138
EcoICRI GAG'CTC
Cuts at: 2262 2262
Size: 6220
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2108 2525 2701 3174 3282 4283 4880
Size: 393 476 417 176 473 108 1001 597
Cuts at: 4880 5667 5935 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1001 787 597 476 473 417 393
268 176 108
EcoO109I rG'GnC_Cy
Cuts at: 2079 2154 2269 2371 2868 2079
Size: 75 115 102 497 5431
Fragments arranged by size:
5431 497 115 102 75
EcoRI G'AATT_C
Cuts at: 3529 3907 3529
Size: 378 5842
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2944 2944
Size: 6220
FspI TGC'GCA
Cuts at: 2204 2276 3470 4726 2204
Size: 72 1194 1256 3698
Fragments arranged by size:
3698 1256 1194 72
FspAI rTGC'GCAy
Cuts at: 2204 2204
Size: 6220
HaeII r_GCGC'y
Cuts at: 1678 5367 1678
Size: 3689 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2345 2377 2852 2884 2999
Size: 32 135 32 773 32 475 32 115
Cuts at: 2999 3031 4861 4893 4935 4967 1373
Size: 32 1830 32 42 32 2626
Fragments arranged by size:
2626 1830 773 475 135 115 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3564 3810 669
Size: 988 281 1626 246 3079
Fragments arranged by size:
3079 1626 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6220
HpaI GTT'AAC
Cuts at: 3810 3810
Size: 6220
KpnI G_GTAC'C
Cuts at: 3545 3545
Size: 6220
MfeI C'AATT_G
Cuts at: 2904 3819 2904
Size: 915 5305
MluI A'CGCG_T
Cuts at: 6096 6096
Size: 6220
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5334 5518 1305
Size: 4029 184 2007
Fragments arranged by size:
4029 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4398
Fragments arranged by size:
4398 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2463 2836 3029 3176 4178 4537 4696
Size: 648 1297 373 193 147 1002 359 159
Cuts at: 4696 518
Size: 2042
Fragments arranged by size:
2042 1297 1002 648 373 359 193 159
147
MspA1I CmG'CkG
Cuts at: 1606 2067 2118 2679 2836 3286 3636 4313 5461
Size: 461 51 561 157 450 350 677 1148
Cuts at: 5461 5706 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 561 461 450 350 245
157 51
NaeI GCC'GGC
Cuts at: 2062 2401 2062
Size: 339 5881
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4729
Fragments arranged by size:
4729 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6220
NgoMIV G'CCGG_C
Cuts at: 2060 2399 2060
Size: 339 5881
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6220
NotI GC'GGCC_GC
Cuts at: 3667 3667
Size: 6220
NspI r_CATG'y
Cuts at: 1132 1229 3049 3578 5123 1132
Size: 97 1820 529 1545 2229
Fragments arranged by size:
2229 1820 1545 529 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6220
PciI A'CATG_T
Cuts at: 5119 5119
Size: 6220
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2488 3381 1108
Size: 1380 893 3947
Fragments arranged by size:
3947 1380 893
PfoI T'CCnGG_A
Cuts at: 2541 2541
Size: 6220
PmeI GTTT'AAAC
Cuts at: 3524 3524
Size: 6220
PmlI CAC'GTG
Cuts at: 2139 2139
Size: 6220
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4289 4321 5802 5834 4289
Size: 32 1481 32 4675
Fragments arranged by size:
4675 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2269 2868 2269
Size: 599 5621
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6220
PsiI TTA'TAA
Cuts at: 3790 3790
Size: 6220
PspOMI G'GGCC_C
Cuts at: 2078 2371 2078
Size: 293 5927
PstI C_TGCA'G
Cuts at: 830 3572 830
Size: 2742 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6220
PvuII CAG'CTG
Cuts at: 2118 2836 3286 2118
Size: 718 450 5052
Fragments arranged by size:
5052 718 450
SacI G_AGCT'C
Cuts at: 2264 2264
Size: 6220
SalI G'TCGA_C
Cuts at: 1936 3562 1936
Size: 1626 4594
SanDI GG'GwC_CC
Cuts at: 2868 2868
Size: 6220
SbfI CC_TGCA'GG
Cuts at: 3572 3572
Size: 6220
ScaI AGT'ACT
Cuts at: 3159 4468 6180 3159
Size: 1309 1712 3199
Fragments arranged by size:
3199 1712 1309
SfcI C'TryA_G
Cuts at: 826 1045 3568 4703 5384 5575 826
Size: 219 2523 1135 681 191 1471
Fragments arranged by size:
2523 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6220
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6220
SmaI CCC'GGG
Cuts at: 1872 2084 3547 1872
Size: 212 1463 4545
Fragments arranged by size:
4545 1463 212
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2320 2342 4324 5225 5487 820
Size: 197 926 377 22 1982 901 262 1553
Fragments arranged by size:
1982 1553 926 901 377 262 197 22
SnaBI TAC'GTA
Cuts at: 493 4011 493
Size: 3518 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6220
SphI G_CATG'C
Cuts at: 1132 3578 1132
Size: 2446 3774
SspI AAT'ATT
Cuts at: 5 52 2709 4144 5
Size: 47 2657 1435 2081
Fragments arranged by size:
2657 2081 1435 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6220
StyI C'CwwG_G
Cuts at: 513 1065 2004 2788 2980 3659 513
Size: 552 939 784 192 679 3074
Fragments arranged by size:
3074 939 784 679 552 192
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2297 2993 4247 4264 4417 4602 5047
Size: 1255 717 696 1254 17 153 185 445
Cuts at: 5047 325
Size: 1498
Fragments arranged by size:
1498 1255 1254 717 696 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3157 4466 6178
Size: 274 80 33 51 1443 1180 1309 1712
Cuts at: 6178 96
Size: 138
Fragments arranged by size:
1712 1443 1309 1180 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2674 2706 3176 3208 6180 6212 2674
Size: 32 470 32 2972 32 2682
Fragments arranged by size:
2972 2682 470 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6220
XbaI T'CTAG_A
Cuts at: 3556 3556
Size: 6220
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2176 2224 2987 3061 3142 2176
Size: 48 763 74 81 5254
Fragments arranged by size:
5254 763 81 74 48
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6220
XmaI C'CCGG_G
Cuts at: 1870 2082 3545 1870
Size: 212 1463 4545
Fragments arranged by size:
4545 1463 212
XmnI GAAnn'nnTTC
Cuts at: 2246 2708 4349 2246
Size: 462 1641 4117
Fragments arranged by size:
4117 1641 462
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4028 276
Size: 53 83 186 936 2494 2468
Fragments arranged by size:
2494 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SacI SalI SanDI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI BsiWI BsmBI BstXI BstZ17I EcoNI EcoRV FseI
KasI NarI NruI NsiI PacI PspXI PsrI RsrII
SacII SapI SexAI SfiI SfoI SrfI SwaI