Restriction map for
pFN21AB4514
(Circular) (Six-base) MAPSORT of: pFN21AB4514.seq Check: 1125 from: 1 to: 9565
pFN21AB4514 9565 bp
With 182 enzymes: SgfI *
January 16, 2009 15:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3075 4625 5103 3075
Size: 1550 478 7537
Fragments arranged by size:
7537 1550 478
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 7375 278
Size: 53 83 186 936 5839 2468
Fragments arranged by size:
5839 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2202 4980 6078 6908 1937
Size: 265 2778 1098 830 4594
Fragments arranged by size:
4594 2778 1098 830 265
Acc65I G'GTAC_C
Cuts at: 6709 6886 6709
Size: 177 9388
AclI AA'CG_TT
Cuts at: 1563 6636 7692 8065 1563
Size: 5073 1056 373 3063
Fragments arranged by size:
5073 3063 1056 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2264 2405 3290 5348 5484 6135 7628 9012
Size: 632 141 885 2058 136 651 1493 1384
Cuts at: 9012 9280 1632
Size: 268 1917
Fragments arranged by size:
2058 1917 1493 1384 885 651 632 268
141 136
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 9368
AflIII A'CryG_T
Cuts at: 1203 3693 4064 4566 6427 8464 9441 1203
Size: 2490 371 502 1861 2037 977 1327
Fragments arranged by size:
2490 2037 1861 1327 977 502 371
AgeI A'CCGG_T
Cuts at: 8396 8396
Size: 9565
AhdI GACnn_n'nnGTC
Cuts at: 5617 5941 8294 5617
Size: 324 2353 6888
Fragments arranged by size:
6888 2353 324
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 9565
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4716 1184
Size: 3532 6033
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2326 2358 3518 3550 5478 5510 2326
Size: 32 1160 32 1928 32 6381
Fragments arranged by size:
6381 1928 1160 32 32 32
AlwNI CAG_nnn'CTG
Cuts at: 3100 3825 4971 5200 5766 5789 7308 8880 3100
Size: 725 1146 229 566 23 1519 1572 3785
Fragments arranged by size:
3785 1572 1519 1146 725 566 229 23
ApaI G_GGCC'C
Cuts at: 2475 4083 4160 6864 2475
Size: 1608 77 2704 5176
Fragments arranged by size:
5176 2704 1608 77
ApaLI G'TGCA_C
Cuts at: 3056 7622 8778 3056
Size: 4566 1156 3843
Fragments arranged by size:
4566 3843 1156
ApoI r'AATT_y
Cuts at: 1493 4009 5522 6874 7100 7252 9361 1493
Size: 2516 1513 1352 226 152 2109 1697
Fragments arranged by size:
2516 2109 1697 1513 1352 226 152
AseI AT'TA_AT
Cuts at: 160 1032 8119 160
Size: 872 7087 1606
Fragments arranged by size:
7087 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9565
AvaI C'yCGr_G
Cuts at: 1870 1943 2124 2208 2258 2477 2576 4001 4213
Size: 73 181 84 50 219 99 1425 212
Cuts at: 4213 5647 5694 5826 6890 1870
Size: 1434 47 132 1064 4545
Fragments arranged by size:
4545 1434 1425 1064 219 212 181 132
99 84 73 50 47
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 6701 6734 6701
Size: 33 9532
BamHI G'GATC_C
Cuts at: 2562 3857 6485 6895 7262 2562
Size: 1295 2628 410 367 4865
Fragments arranged by size:
4865 2628 1295 410 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2182 2317 2874 3063 4186
Size: 325 217 627 395 135 557 189 1123
Cuts at: 4186 4510 4700 5335 6709 6886 8341 618
Size: 324 190 635 1374 177 1455 1842
Fragments arranged by size:
1842 1455 1374 1123 635 627 557 395
325 324 217 190 189 177 135
BanII G_rGCy'C
Cuts at: 1391 2022 2066 2096 2475 3323 3641 3654 4083
Size: 631 44 30 379 848 318 13 429
Cuts at: 4083 4160 5231 5598 6681 6864 6884 1391
Size: 77 1071 367 1083 183 20 4072
Fragments arranged by size:
4072 1083 1071 848 631 429 379 367
318 183 77 44 30 20 13
BbeI G_GCGC'C
Cuts at: 2321 2878 2321
Size: 557 9008
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 5459 6123 928
Size: 971 3560 664 4370
Fragments arranged by size:
4370 3560 971 664
BbvCI CC'TCA_GC
Cuts at: 2980 5049 2980
Size: 2069 7496
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5675 5709 7756 7790 1903
Size: 34 3738 34 2047 34 3678
Fragments arranged by size:
3738 3678 2047 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6814 7457 8667 6814
Size: 643 1210 7712
Fragments arranged by size:
7712 1210 643
BclI T'GATC_A
Cuts at: 1548 4518 5511 1548
Size: 2970 993 5602
Fragments arranged by size:
5602 2970 993
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3872 4553 5138
Size: 107 122 71 631 605 2200 681 585
Cuts at: 5138 6244 8176 136
Size: 1106 1932 1525
Fragments arranged by size:
2200 1932 1525 1106 681 631 605 585
122 107 71
BglII A'GATC_T
Cuts at: 2658 4176 9560 2658
Size: 1518 5384 2663
Fragments arranged by size:
5384 2663 1518
BlpI GC'TnA_GC
Cuts at: 5243 6982 5243
Size: 1739 7826
Bme1580I G_kGCm'C
Cuts at: 1790 2187 2475 3060 3391 4083 4160 4515 6864
Size: 397 288 585 331 692 77 355 2349
Cuts at: 6864 7626 8782 1790
Size: 762 1156 2573
Fragments arranged by size:
2573 2349 1156 762 692 585 397 355
331 288 77
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 9565
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4350 4898 6654
Size: 416 53 83 399 24 2933 548 1756
Cuts at: 6654 7296 8254 442
Size: 642 958 1753
Fragments arranged by size:
2933 1756 1753 958 642 548 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2279 1056
Size: 1223 8342
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2914 3146 4612 2914
Size: 232 1466 7867
Fragments arranged by size:
7867 1466 232
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2453 2486 2816 2846 3129 3155 3211 3335
Size: 1214 33 330 30 283 26 56 124
Cuts at: 3335 4319 4607 5612 6041 6629 6822 8225 1239
Size: 984 288 1005 429 588 193 1403 2579
Fragments arranged by size:
2579 1403 1214 1005 984 588 429 330
288 283 193 124 56 33 30 26
Bpu10I CC'TnA_GC
Cuts at: 2300 2980 4133 5049 2300
Size: 680 1153 916 6816
Fragments arranged by size:
6816 1153 916 680
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3703 3793 5600 7690 8555 8853 3703
Size: 90 1807 2090 865 298 4415
Fragments arranged by size:
4415 2090 1807 865 298 90
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2332 5604 5627 6286 6391 8228 882
Size: 617 833 3272 23 659 105 1837 2219
Fragments arranged by size:
3272 2219 1837 833 659 617 105 23
BsaAI yAC'GTr
Cuts at: 493 1204 4483 5689 6430 7356 493
Size: 711 3279 1206 741 926 2702
Fragments arranged by size:
3279 2702 1206 926 741 711
BsaBI GATnn'nnATC
Cuts at: 9559 9559
Size: 9565
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2318 2588 2875 3187
Size: 53 83 186 936 785 270 287 312
Cuts at: 3187 4347 5058 7372 7754 8389 275
Size: 1160 711 2314 382 635 1451
Fragments arranged by size:
2314 1451 1160 936 785 711 635 382
312 287 270 186 83 53
BsaWI w'CCGG_w
Cuts at: 4625 4706 5411 6081 7998 8396 8670 8817 9228
Size: 81 705 670 1917 398 274 147 411
Cuts at: 9228 4625
Size: 4962
Fragments arranged by size:
4962 1917 705 670 411 398 274 147
81
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3013 3043 3872 3902 4109
Size: 30 165 30 1610 30 829 30 207
Cuts at: 4109 4139 4494 4524 1178
Size: 30 355 30 6219
Fragments arranged by size:
6219 1610 829 355 207 165 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2224 2459 2906 3034 3489 3794 4473 4476
Size: 1038 235 447 128 455 305 679 3
Cuts at: 4476 4588 4780 4815 5302 5650 6261 6317 6320
Size: 112 192 35 487 348 611 56 3
Cuts at: 6320 6337 1186
Size: 17 4414
Fragments arranged by size:
4414 1038 679 611 487 455 447 348
305 235 192 128 112 56 35 17
3 3
BseYI C'CCAG_C
Cuts at: 2098 2308 2437 2637 3093 4282 4906 5996 6316
Size: 210 129 200 456 1189 624 1090 320
Cuts at: 6316 8768 9264 2098
Size: 2452 496 2399
Fragments arranged by size:
2452 2399 1189 1090 624 496 456 320
210 200 129
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2821 3022 3609 5669 6145 1163
Size: 573 1085 201 587 2060 476 4583
Fragments arranged by size:
4583 2060 1085 587 573 476 201
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 6414 7015 7776 8804 1540
Size: 275 186 4413 601 761 1028 2301
Fragments arranged by size:
4413 2301 1028 761 601 275 186
BsiHKAI G_wGCw'C
Cuts at: 3036 3060 3171 3323 3641 3654 4790 5474 5598
Size: 24 111 152 318 13 1136 684 124
Cuts at: 5598 7626 7711 8782 3036
Size: 2028 85 1071 3819
Fragments arranged by size:
3819 2028 1136 1071 684 318 152 124
111 85 24 13
BsiWI C'GTAC_G
Cuts at: 6522 6522
Size: 9565
BsmI GAATG_Cn'
Cuts at: 5421 6440 7076 7169 5421
Size: 1019 636 93 7817
Fragments arranged by size:
7817 1019 636 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3055 5065 3055
Size: 2010 7555
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2022 2066 2096 2187 2475 3036 3060
Size: 399 232 44 30 91 288 561 24
Cuts at: 3060 3171 3323 3391 3641 3654 4083 4160 4515
Size: 111 152 68 250 13 429 77 355
Cuts at: 4515 4790 5231 5474 5598 6681 6864 6884 7626
Size: 275 441 243 124 1083 183 20 742
Cuts at: 7626 7711 8782 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1083 1071 742 561 441 429 399
355 288 275 250 243 232 183 152
124 111 91 85 77 68 44 30
24 20 13
BspEI T'CCGG_A
Cuts at: 4625 5411 9228 4625
Size: 786 3817 4962
Fragments arranged by size:
4962 3817 786
BspHI T'CATG_A
Cuts at: 5169 5268 7454 9184 5169
Size: 99 2186 1730 5550
Fragments arranged by size:
5550 2186 1730 99
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3075 3499 4625 5103 6920 844
Size: 2231 424 1126 478 1817 3489
Fragments arranged by size:
3489 2231 1817 1126 478 424
BsrBI CCG'CTC
Cuts at: 1394 1823 7452 1394
Size: 429 5629 3507
Fragments arranged by size:
5629 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3087 4521 4831 5700 5751 8060 8234
Size: 1183 1839 1434 310 869 51 2309 174
Cuts at: 8234 65
Size: 1396
Fragments arranged by size:
2309 1839 1434 1396 1183 869 310 174
51
BsrFI r'CCGG_y
Cuts at: 1700 1810 2078 2314 2339 4507 6409 8209 8396
Size: 110 268 236 25 2168 1902 1800 187
Cuts at: 8396 1700
Size: 2869
Fragments arranged by size:
2869 2168 1902 1800 268 236 187 110
25
BsrGI T'GTAC_A
Cuts at: 96 4317 4899 96
Size: 4221 582 4762
Fragments arranged by size:
4762 4221 582
BssHII G'CGCG_C
Cuts at: 1925 2086 1925
Size: 161 9404
BssSI C'ACGA_G
Cuts at: 5135 6587 7625 8637 5135
Size: 1452 1038 1012 6063
Fragments arranged by size:
6063 1452 1038 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2428 5209 5572 794
Size: 1634 2781 363 4787
Fragments arranged by size:
4787 2781 1634 363
BstBI TT'CG_AA
Cuts at: 8369 8369
Size: 9565
BstEII G'GTnAC_C
Cuts at: 1184 5122 6596 1184
Size: 3938 1474 4153
Fragments arranged by size:
4153 3938 1474
BstXI CCAn_nnnn'nTGG
Cuts at: 2425 3898 5215 5886 6142 2425
Size: 1473 1317 671 256 5848
Fragments arranged by size:
5848 1473 1317 671 256
BstYI r'GATC_y
Cuts at: 1972 2562 2658 3126 3857 3933 4176 6485 6895
Size: 590 96 468 731 76 243 2309 410
Cuts at: 6895 7262 7647 7664 9116 9202 9214 9560 1972
Size: 367 385 17 1452 86 12 346 1977
Fragments arranged by size:
2309 1977 1452 731 590 468 410 385
367 346 243 96 86 76 17 12
BstZ17I GTA'TAC
Cuts at: 6079 6079
Size: 9565
Bsu36I CC'TnA_GG
Cuts at: 2852 4792 6258 2852
Size: 1940 1466 6159
Fragments arranged by size:
6159 1940 1466
BtgI C'CryG_G
Cuts at: 513 1065 2004 2049 2266 2619 3011 4084 4267
Size: 552 939 45 217 353 392 1073 183
Cuts at: 4267 4930 5032 5339 5374 6414 6481 513
Size: 663 102 307 35 1040 67 3597
Fragments arranged by size:
3597 1073 1040 939 663 552 392 353
307 217 183 102 67 45 35
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2762 487
Size: 683 56 1536 7290
Fragments arranged by size:
7290 1536 683 56
BtsI GCAGTG_nn'
Cuts at: 2840 3034 3258 3286 3791 6665 7081 7875 7895
Size: 194 224 28 505 2874 416 794 20
Cuts at: 7895 2840
Size: 4510
Fragments arranged by size:
4510 2874 794 505 416 224 194 28
20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 9565
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 9530
DraI TTT'AAA
Cuts at: 4914 6869 4914
Size: 1955 7610
DraIII CAC_nnn'GTG
Cuts at: 4273 4273
Size: 9565
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 8572 809
Size: 507 7256 1802
Fragments arranged by size:
7256 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2080 3045 5139
Size: 54 1425 50 275 18 250 965 2094
Cuts at: 5139 5667 5799 6411 7012 7901 9530 8
Size: 528 132 612 601 889 1629 43
Fragments arranged by size:
2094 1629 1425 965 889 612 601 528
275 250 132 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 6411 7012 1537
Size: 275 4599 601 4090
Fragments arranged by size:
4599 4090 601 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2153 2198 2657 5788 6216 6222 6688 7495
Size: 800 45 459 3131 428 6 466 807
Cuts at: 7495 1353
Size: 3423
Fragments arranged by size:
3423 3131 807 800 466 459 428 45
6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2555 4470 4821 8152 8526 8672
Size: 1064 138 966 1915 351 3331 374 146
Cuts at: 8672 387
Size: 1280
Fragments arranged by size:
3331 1915 1280 1064 966 374 351 146
138
EcoICRI GAG'CTC
Cuts at: 3321 3639 3652 5596 3321
Size: 318 13 1944 7290
Fragments arranged by size:
7290 1944 318 13
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2264 2405 2453 2486 2816 2846 3129
Size: 393 632 141 48 33 330 30 283
Cuts at: 3129 3155 3211 3290 3335 4319 4607 5348 5484
Size: 26 56 79 45 984 288 741 136
Cuts at: 5484 5612 6041 6135 6629 6822 7628 8225 9012
Size: 128 429 94 494 193 806 597 787
Cuts at: 9012 9280 1239
Size: 268 1524
Fragments arranged by size:
1524 984 806 787 741 632 597 494
429 393 330 288 283 268 193 141
136 128 94 79 56 48 45 33
30 26
EcoNI CCTnn'n_nnAGG
Cuts at: 3079 4301 5330 3079
Size: 1222 1029 7314
Fragments arranged by size:
7314 1222 1029
EcoO109I rG'GnC_Cy
Cuts at: 2212 2472 3516 4080 4156 4157 4545 5786 6169
Size: 260 1044 564 76 1 388 1241 383
Cuts at: 6169 6253 6660 2212
Size: 84 407 5117
Fragments arranged by size:
5117 1241 1044 564 407 388 383 260
84 76 1
EcoRI G'AATT_C
Cuts at: 6874 7252 6874
Size: 378 9187
FseI GG_CCGG'CC
Cuts at: 6413 6413
Size: 9565
FspI TGC'GCA
Cuts at: 2402 8071 2402
Size: 5669 3896
HaeII r_GCGC'y
Cuts at: 1678 2321 2878 8712 1678
Size: 643 557 5834 2531
Fragments arranged by size:
5834 2531 643 557
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2146 2178 2547 2579 3127
Size: 32 135 32 574 32 369 32 548
Cuts at: 3127 3159 3872 3904 5045 5077 5603 5626 5635
Size: 32 713 32 1141 32 526 23 9
Cuts at: 5635 5658 5927 5959 6076 6108 8206 8238 8280
Size: 23 269 32 117 32 2098 32 42
Cuts at: 8280 8312 1373
Size: 32 2626
Fragments arranged by size:
2626 2098 1141 713 574 548 526 369
269 135 117 42 32 32 32 32
32 32 32 32 32 32 32 23
23 9
HincII GTy'rAC
Cuts at: 669 1657 1938 2173 6909 7155 669
Size: 988 281 235 4736 246 3079
Fragments arranged by size:
4736 3079 988 281 246 235
HindIII A'AGCT_T
Cuts at: 748 748
Size: 9565
HpaI GTT'AAC
Cuts at: 7155 7155
Size: 9565
KasI G'GCGC_C
Cuts at: 2317 2874 2317
Size: 557 9008
KpnI G_GTAC'C
Cuts at: 6713 6890 6713
Size: 177 9388
MfeI C'AATT_G
Cuts at: 7164 7164
Size: 9565
MluI A'CGCG_T
Cuts at: 9441 9441
Size: 9565
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2571 3140 3314 4152 6477 8679 8863 1305
Size: 1266 569 174 838 2325 2202 184 2007
Fragments arranged by size:
2325 2202 2007 1266 838 569 184 174
MscI TGG'CCA
Cuts at: 10 64 1489 1832 5669 5801 10
Size: 54 1425 343 3837 132 3774
Fragments arranged by size:
3837 3774 1425 343 132 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4256 5174 5213 5555 7523 7882 8041
Size: 648 3090 918 39 342 1968 359 159
Cuts at: 8041 518
Size: 2042
Fragments arranged by size:
3090 2042 1968 918 648 359 342 159
39
MspA1I CmG'CkG
Cuts at: 1606 2038 2057 2102 2116 2268 2296 2621 2984
Size: 432 19 45 14 152 28 325 363
Cuts at: 2984 3097 4077 5341 6320 6377 6981 7658 8806
Size: 113 980 1264 979 57 604 677 1148
Cuts at: 8806 9051 1606
Size: 245 2120
Fragments arranged by size:
2120 1264 1148 980 979 677 604 432
363 325 245 152 113 57 45 28
19 14
NaeI GCC'GGC
Cuts at: 2316 6411 2316
Size: 4095 5470
NarI GG'CG_CC
Cuts at: 2318 2875 2318
Size: 557 9008
NcoI C'CATG_G
Cuts at: 513 1065 2004 4084 5374 513
Size: 552 939 2080 1290 4704
Fragments arranged by size:
4704 2080 1290 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 9565
NgoMIV G'CCGG_C
Cuts at: 2314 6409 2314
Size: 4095 5470
NheI G'CTAG_C
Cuts at: 1052 2275 1052
Size: 1223 8342
NotI GC'GGCC_GC
Cuts at: 7012 7012
Size: 9565
NspI r_CATG'y
Cuts at: 1132 1229 2704 3755 4068 4315 6923 8468 1132
Size: 97 1475 1051 313 247 2608 1545 2229
Fragments arranged by size:
2608 2229 1545 1475 1051 313 247 97
PasI CC'CwG_GG
Cuts at: 1348 3391 6068 1348
Size: 2043 2677 4845
Fragments arranged by size:
4845 2677 2043
PciI A'CATG_T
Cuts at: 4064 8464 4064
Size: 4400 5165
PflMI CCAn_nnn'nTGG
Cuts at: 1108 5789 6510 1108
Size: 4681 721 4163
Fragments arranged by size:
4681 4163 721
PfoI T'CCnGG_A
Cuts at: 2066 2330 3537 2066
Size: 264 1207 8094
Fragments arranged by size:
8094 1207 264
PmeI GTTT'AAAC
Cuts at: 6869 6869
Size: 9565
PmlI CAC'GTG
Cuts at: 5689 6430 5689
Size: 741 8824
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7634 7666 9147 9179 7634
Size: 32 1481 32 8020
Fragments arranged by size:
8020 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2212 3516 6169 6253 2212
Size: 1304 2653 84 5524
Fragments arranged by size:
5524 2653 1304 84
PshAI GACnn'nnGTC
Cuts at: 1613 4986 1613
Size: 3373 6192
PsiI TTA'TAA
Cuts at: 7135 7135
Size: 9565
PspOMI G'GGCC_C
Cuts at: 2471 4079 4156 6860 2471
Size: 1608 77 2704 5176
Fragments arranged by size:
5176 2704 1608 77
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 6506 6538 6506
Size: 32 9533
PstI C_TGCA'G
Cuts at: 830 2121 2154 3072 3253 3281 3318 3834 3942
Size: 1291 33 918 181 28 37 516 108
Cuts at: 3942 4967 5226 6382 6917 830
Size: 1025 259 1156 535 3478
Fragments arranged by size:
3478 1291 1156 1025 918 535 516 259
181 108 37 33 28
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9565
PvuII CAG'CTG
Cuts at: 2057 2102 2984 3097 6320 6377 2057
Size: 45 882 113 3223 57 5245
Fragments arranged by size:
5245 3223 882 113 57 45
SacI G_AGCT'C
Cuts at: 3323 3641 3654 5598 3323
Size: 318 13 1944 7290
Fragments arranged by size:
7290 1944 318 13
SacII CC_GC'GG
Cuts at: 2269 2622 5342 2269
Size: 353 2720 6492
Fragments arranged by size:
6492 2720 353
SalI G'TCGA_C
Cuts at: 1936 6907 1936
Size: 4971 4594
SapI GCTCTTCn'nnn_
Cuts at: 2198 2198
Size: 9565
SbfI CC_TGCA'GG
Cuts at: 6917 6917
Size: 9565
ScaI AGT'ACT
Cuts at: 7813 9525 7813
Size: 1712 7853
SfcI C'TryA_G
Cuts at: 826 1045 2117 2150 3068 3249 3277 3314 3600
Size: 219 1072 33 918 181 28 37 286
Cuts at: 3600 3830 3938 4041 4204 4963 5222 5836 5851
Size: 230 108 103 163 759 259 614 15
Cuts at: 5851 6378 6567 6913 8048 8729 8920 826
Size: 527 189 346 1135 681 191 1471
Fragments arranged by size:
1471 1135 1072 918 759 681 614 527
346 286 259 230 219 191 189 181
163 108 103 37 33 28 15
SfiI GGCCn_nnn'nGGCC
Cuts at: 4553 4553
Size: 9565
SfoI GGC'GCC
Cuts at: 2319 2876 2319
Size: 557 9008
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9565
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 9565
SmaI CCC'GGG
Cuts at: 1872 2126 2210 2479 4003 6892 1872
Size: 254 84 269 1524 2889 4545
Fragments arranged by size:
4545 2889 1524 269 254 84
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3682 3772 5615 7669 8570 8832
Size: 197 926 1739 90 1843 2054 901 262
Cuts at: 8832 820
Size: 1553
Fragments arranged by size:
2054 1843 1739 1553 926 901 262 197
90
SnaBI TAC'GTA
Cuts at: 493 4483 7356 493
Size: 3990 2873 2702
Fragments arranged by size:
3990 2873 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 9565
SphI G_CATG'C
Cuts at: 1132 6923 1132
Size: 5791 3774
SspI AAT'ATT
Cuts at: 5 52 4945 5919 7489 5
Size: 47 4893 974 1570 2081
Fragments arranged by size:
4893 2081 1570 974 47
StuI AGG'CCT
Cuts at: 1517 2897 3365 5903 1517
Size: 1380 468 2538 5179
Fragments arranged by size:
5179 2538 1380 468
StyI C'CwwG_G
Cuts at: 513 1065 2004 2542 2682 3475 3699 4084 5374
Size: 552 939 538 140 793 224 385 1290
Cuts at: 5374 6249 7004 513
Size: 875 755 3074
Fragments arranged by size:
3074 1290 939 875 793 755 552 538
385 224 140
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3967 4109 4590 5092 5288 6070 6179
Size: 1255 2387 142 481 502 196 782 109
Cuts at: 6179 7592 7609 7762 7947 8392 325
Size: 1413 17 153 185 445 1498
Fragments arranged by size:
2387 1498 1413 1255 782 502 481 445
196 185 153 142 109 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4317 4358 4899
Size: 274 80 33 51 1443 2340 41 541
Cuts at: 4899 5235 6620 7811 9523 96
Size: 336 1385 1191 1712 138
Fragments arranged by size:
2340 1712 1443 1385 1191 541 336 274
138 80 51 41 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3013 3045 4494 4526 4878 4910 9525 9557 3013
Size: 32 1449 32 352 32 4615 32 3021
Fragments arranged by size:
4615 3021 1449 352 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2371 1316
Size: 1055 8510
XbaI T'CTAG_A
Cuts at: 6901 6901
Size: 9565
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2056 3868 4135 5381 2056
Size: 1812 267 1246 6240
Fragments arranged by size:
6240 1812 1246 267
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 9565
XmaI C'CCGG_G
Cuts at: 1870 2124 2208 2477 4001 6890 1870
Size: 254 84 269 1524 2889 4545
Fragments arranged by size:
4545 2889 1524 269 254 84
XmnI GAAnn'nnTTC
Cuts at: 7694 7694
Size: 9565
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 7373 276
Size: 53 83 186 936 5839 2468
Fragments arranged by size:
5839 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI
SacII SalI SapI SbfI ScaI SfcI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BfrBI EcoRV FalI FspAI NruI
NsiI PacI PspXI RsrII SanDI SexAI SrfI SwaI