Restriction map for
pFN21AB4508
(Circular) (Six-base) MAPSORT of: pFN21AB4508.seq Check: 7003 from: 1 to: 5872
pFN21AB4508 5872 bp
With 182 enzymes: SgfI *
October 20, 2009 10:59 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3682 278
Size: 53 83 186 936 2146 2468
Fragments arranged by size:
2468 2146 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3215 1937
Size: 1278 4594
Acc65I G'GTAC_C
Cuts at: 3193 3193
Size: 5872
AclI AA'CG_TT
Cuts at: 1563 3999 4372 1563
Size: 2436 373 3063
Fragments arranged by size:
3063 2436 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3135 3935 5319 5587 1632
Size: 1503 800 1384 268 1917
Fragments arranged by size:
1917 1503 1384 800 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5675
AflIII A'CryG_T
Cuts at: 1203 4771 5748 1203
Size: 3568 977 1327
Fragments arranged by size:
3568 1327 977
AgeI A'CCGG_T
Cuts at: 2120 4703 2120
Size: 2583 3289
AhdI GACnn_n'nnGTC
Cuts at: 4601 4601
Size: 5872
AleI CACnn'nnGTG
Cuts at: 1166 2387 2770 1166
Size: 1221 383 4268
Fragments arranged by size:
4268 1221 383
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5872
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2247 2279 2247
Size: 32 5840
AlwNI CAG_nnn'CTG
Cuts at: 3615 5187 3615
Size: 1572 4300
ApaLI G'TGCA_C
Cuts at: 3929 5085 3929
Size: 1156 4716
ApoI r'AATT_y
Cuts at: 1493 2497 3070 3181 3407 3559 5668 1493
Size: 1004 573 111 226 152 2109 1697
Fragments arranged by size:
2109 1697 1004 573 226 152 111
AseI AT'TA_AT
Cuts at: 160 1032 2351 4426 160
Size: 872 1319 2075 1606
Fragments arranged by size:
2075 1606 1319 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5872
AvaI C'yCGr_G
Cuts at: 1870 1943 3197 1870
Size: 73 1254 4545
Fragments arranged by size:
4545 1254 73
BamHI G'GATC_C
Cuts at: 3202 3569 3202
Size: 367 5505
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3193 4648 618
Size: 325 217 627 1406 1455 1842
Fragments arranged by size:
1842 1455 1406 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2284 2367 3191 1391
Size: 893 83 824 4072
Fragments arranged by size:
4072 893 824 83
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2521 928
Size: 971 622 4279
Fragments arranged by size:
4279 971 622
BbvCI CC'TCA_GC
Cuts at: 2997 2997
Size: 5872
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4063 4097 1903
Size: 34 2126 34 3678
Fragments arranged by size:
3678 2126 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3764 4974 3764
Size: 1210 4662
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5872
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4483 136
Size: 107 122 71 631 605 2811 1525
Fragments arranged by size:
2811 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2700 5867 2700
Size: 3167 2705
BlpI GC'TnA_GC
Cuts at: 2333 2507 3289 2333
Size: 174 782 4916
Fragments arranged by size:
4916 782 174
Bme1580I G_kGCm'C
Cuts at: 1790 2662 2953 3933 5089 1790
Size: 872 291 980 1156 2573
Fragments arranged by size:
2573 1156 980 872 291
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5872
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3603 4561 442
Size: 416 53 83 399 24 2186 958 1753
Fragments arranged by size:
2186 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5872
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2156 2684 4532 1239
Size: 917 528 1848 2579
Fragments arranged by size:
2579 1848 917 528
Bpu10I CC'TnA_GC
Cuts at: 2997 2997
Size: 5872
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2470 3997 4862 5160 2470
Size: 1527 865 298 3182
Fragments arranged by size:
3182 1527 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2314 4535 882
Size: 617 815 2221 2219
Fragments arranged by size:
2221 2219 815 617
BsaAI yAC'GTr
Cuts at: 493 1204 3663 493
Size: 711 2459 2702
Fragments arranged by size:
2702 2459 711
BsaBI GATnn'nnATC
Cuts at: 5866 5866
Size: 5872
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3679 4061 4696 275
Size: 53 83 186 936 2146 382 635 1451
Fragments arranged by size:
2146 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2120 4305 4703 4977 5124 5535 2120
Size: 2185 398 274 147 411 2457
Fragments arranged by size:
2457 2185 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2614 2644 1178
Size: 30 165 30 1211 30 4406
Fragments arranged by size:
4406 1211 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2749 1186
Size: 1563 4309
BseYI C'CCAG_C
Cuts at: 2296 2800 5075 5571 2296
Size: 504 2275 496 2597
Fragments arranged by size:
2597 2275 504 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5299
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3322 4083 5111 1540
Size: 275 186 1321 761 1028 2301
Fragments arranged by size:
2301 1321 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2172 3933 4018 5089 2172
Size: 1761 85 1071 2955
Fragments arranged by size:
2955 1761 1071 85
BsmI GAATG_Cn'
Cuts at: 3383 3476 3383
Size: 93 5779
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2172 2284 2367 2662 2953 3191 3933
Size: 399 382 112 83 295 291 238 742
Cuts at: 3933 4018 5089 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 382 295 291 238
112 85 83
BspEI T'CCGG_A
Cuts at: 5535 5535
Size: 5872
BspHI T'CATG_A
Cuts at: 3057 3761 5491 3057
Size: 704 1730 3438
Fragments arranged by size:
3438 1730 704
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3144 3227 844
Size: 2300 83 3489
Fragments arranged by size:
3489 2300 83
BsrBI CCG'CTC
Cuts at: 1394 1823 3759 1394
Size: 429 1936 3507
Fragments arranged by size:
3507 1936 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2795 2886 2898 4367 4541 65
Size: 1183 1547 91 12 1469 174 1396
Fragments arranged by size:
1547 1469 1396 1183 174 91 12
BsrFI r'CCGG_y
Cuts at: 1700 1810 2120 4516 4703 1700
Size: 110 310 2396 187 2869
Fragments arranged by size:
2869 2396 310 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5872
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5872
BssSI C'ACGA_G
Cuts at: 3932 4944 3932
Size: 1012 4860
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5872
BstBI TT'CG_AA
Cuts at: 4676 4676
Size: 5872
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5872
BstXI CCAn_nnnn'nTGG
Cuts at: 2320 2389 2320
Size: 69 5803
BstYI r'GATC_y
Cuts at: 1972 2700 3202 3569 3954 3971 5423 5509 5521
Size: 728 502 367 385 17 1452 86 12
Cuts at: 5521 5867 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 728 502 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2163 2720 513
Size: 552 939 159 557 3665
Fragments arranged by size:
3665 939 557 552 159
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5133
Fragments arranged by size:
5133 683 56
BtsI GCAGTG_nn'
Cuts at: 3388 4182 4202 3388
Size: 794 20 5058
Fragments arranged by size:
5058 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5872
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5837
DraI TTT'AAA
Cuts at: 3176 3176
Size: 5872
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2525 4879 809
Size: 507 1209 2354 1802
Fragments arranged by size:
2354 1802 1209 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3036 3319 4208
Size: 54 1425 50 275 18 1206 283 889
Cuts at: 4208 5837 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1206 889 283 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3319 1537
Size: 275 1507 4090
Fragments arranged by size:
4090 1507 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3802 1353
Size: 2449 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4459 4833 4979 387
Size: 1064 138 2870 374 146 1280
Fragments arranged by size:
2870 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2156 2684 3135 3935 4532 5319 5587
Size: 393 524 528 451 800 597 787 268
Cuts at: 5587 1239
Size: 1524
Fragments arranged by size:
1524 800 787 597 528 524 451 393
268
EcoO109I rG'GnC_Cy
Cuts at: 2518 2518
Size: 5872
EcoRI G'AATT_C
Cuts at: 2497 3181 3559 2497
Size: 684 378 4810
Fragments arranged by size:
4810 684 378
FspI TGC'GCA
Cuts at: 4378 4378
Size: 5872
HaeII r_GCGC'y
Cuts at: 1678 2915 5019 1678
Size: 1237 2104 2531
Fragments arranged by size:
2531 2104 1237
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4513 4545 4587 4619 1373
Size: 32 135 32 2941 32 42 32 2626
Fragments arranged by size:
2941 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2221 3216 3462 669
Size: 988 281 283 995 246 3079
Fragments arranged by size:
3079 995 988 283 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5872
HpaI GTT'AAC
Cuts at: 3462 3462
Size: 5872
KpnI G_GTAC'C
Cuts at: 3197 3197
Size: 5872
MfeI C'AATT_G
Cuts at: 3471 3471
Size: 5872
MluI A'CGCG_T
Cuts at: 5748 5748
Size: 5872
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2983 3099 4986 5170 1305
Size: 1678 116 1887 184 2007
Fragments arranged by size:
2007 1887 1678 184 116
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3038 10
Size: 54 1425 343 1206 2844
Fragments arranged by size:
2844 1425 1206 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2318 2387 2770 2891 3830 4189 4348
Size: 648 1152 69 383 121 939 359 159
Cuts at: 4348 518
Size: 2042
Fragments arranged by size:
2042 1152 939 648 383 359 159 121
69
MspA1I CmG'CkG
Cuts at: 1606 2813 3288 3965 5113 5358 1606
Size: 1207 475 677 1148 245 2120
Fragments arranged by size:
2120 1207 1148 677 475 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2720 513
Size: 552 939 716 3665
Fragments arranged by size:
3665 939 716 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5872
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5872
NotI GC'GGCC_GC
Cuts at: 3319 3319
Size: 5872
NspI r_CATG'y
Cuts at: 1132 1229 2783 3230 4775 1132
Size: 97 1554 447 1545 2229
Fragments arranged by size:
2229 1554 1545 447 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5872
PciI A'CATG_T
Cuts at: 4771 4771
Size: 5872
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2935 1108
Size: 1827 4045
PmeI GTTT'AAAC
Cuts at: 3176 3176
Size: 5872
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3941 3973 5454 5486 3941
Size: 32 1481 32 4327
Fragments arranged by size:
4327 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2518 2518
Size: 5872
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5872
PsiI TTA'TAA
Cuts at: 3442 3442
Size: 5872
PstI C_TGCA'G
Cuts at: 830 3224 830
Size: 2394 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5872
SalI G'TCGA_C
Cuts at: 1936 3214 1936
Size: 1278 4594
SbfI CC_TGCA'GG
Cuts at: 3224 3224
Size: 5872
ScaI AGT'ACT
Cuts at: 2397 4120 5832 2397
Size: 1723 1712 2437
Fragments arranged by size:
2437 1723 1712
SexAI A'CCwGG_T
Cuts at: 2064 2064
Size: 5872
SfcI C'TryA_G
Cuts at: 826 1045 2625 3220 4355 5036 5227 826
Size: 219 1580 595 1135 681 191 1471
Fragments arranged by size:
1580 1471 1135 681 595 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5872
SgrAI Cr'CCGG_yG
Cuts at: 1700 2120 1700
Size: 420 5452
SmaI CCC'GGG
Cuts at: 1872 3199 1872
Size: 1327 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2485 3976 4877 5139 820
Size: 197 926 542 1491 901 262 1553
Fragments arranged by size:
1553 1491 926 901 542 262 197
SnaBI TAC'GTA
Cuts at: 493 3663 493
Size: 3170 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5872
SphI G_CATG'C
Cuts at: 1132 2783 3230 1132
Size: 1651 447 3774
Fragments arranged by size:
3774 1651 447
SspI AAT'ATT
Cuts at: 5 52 3796 5
Size: 47 3744 2081
Fragments arranged by size:
3744 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5872
StyI C'CwwG_G
Cuts at: 513 1065 2004 2720 3311 513
Size: 552 939 716 591 3074
Fragments arranged by size:
3074 939 716 591 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3899 3916 4069 4254 4699 325
Size: 1255 2319 17 153 185 445 1498
Fragments arranged by size:
2319 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2395 4118 5830
Size: 274 80 33 51 1443 418 1723 1712
Cuts at: 5830 96
Size: 138
Fragments arranged by size:
1723 1712 1443 418 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5832 5864 5832
Size: 32 5840
Tth111I GACn'n_nGTC
Cuts at: 1316 2820 1316
Size: 1504 4368
XbaI T'CTAG_A
Cuts at: 3208 3208
Size: 5872
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2640 3018 3151 2640
Size: 378 133 5361
Fragments arranged by size:
5361 378 133
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5872
XmaI C'CCGG_G
Cuts at: 1870 3197 1870
Size: 1327 4545
XmnI GAAnn'nnTTC
Cuts at: 4001 4001
Size: 5872
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3680 276
Size: 53 83 186 936 2146 2468
Fragments arranged by size:
2468 2146 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI SalI SbfI ScaI SexAI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI
PvuII RsrII SacI SacII SanDI SapI SfiI SfoI
SrfI SwaI