Restriction map for
pFN21AB4506
(Circular) (Six-base) MAPSORT of: pFN21AB4506.seq Check: 9089 from: 1 to: 6670
pFN21AB4506 6670 bp
With 182 enzymes: SgfI *
February 27, 2009 14:37 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4480 278
Size: 53 83 186 936 2944 2468
Fragments arranged by size:
2944 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4013 1937
Size: 2076 4594
Acc65I G'GTAC_C
Cuts at: 2527 3991 2527
Size: 1464 5206
AclI AA'CG_TT
Cuts at: 1563 4797 5170 1563
Size: 3234 373 3063
Fragments arranged by size:
3234 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3666 4733 6117 6385 1632
Size: 2034 1067 1384 268 1917
Fragments arranged by size:
2034 1917 1384 1067 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6473
AflIII A'CryG_T
Cuts at: 1203 5569 6546 1203
Size: 4366 977 1327
Fragments arranged by size:
4366 1327 977
AgeI A'CCGG_T
Cuts at: 5501 5501
Size: 6670
AhdI GACnn_n'nnGTC
Cuts at: 5399 5399
Size: 6670
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6670
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3413 1184
Size: 2229 4441
AlwNI CAG_nnn'CTG
Cuts at: 2405 2558 4413 5985 2405
Size: 153 1855 1572 3090
Fragments arranged by size:
3090 1855 1572 153
ApaI G_GGCC'C
Cuts at: 3864 3864
Size: 6670
ApaLI G'TGCA_C
Cuts at: 4727 5883 4727
Size: 1156 5514
ApoI r'AATT_y
Cuts at: 1493 3979 4205 4357 6466 1493
Size: 2486 226 152 2109 1697
Fragments arranged by size:
2486 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5224 160
Size: 872 4192 1606
Fragments arranged by size:
4192 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6670
AvaI C'yCGr_G
Cuts at: 1870 1943 3507 3516 3676 3995 1870
Size: 73 1564 9 160 319 4545
Fragments arranged by size:
4545 1564 319 160 73 9
AvrII C'CTAG_G
Cuts at: 2491 2491
Size: 6670
BamHI G'GATC_C
Cuts at: 2738 4000 4367 2738
Size: 1262 367 5041
Fragments arranged by size:
5041 1262 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2527 3991 5446 618
Size: 325 217 627 740 1464 1455 1842
Fragments arranged by size:
1842 1464 1455 740 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3864 3989 1391
Size: 2473 125 4072
Fragments arranged by size:
4072 2473 125
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3075 3261 3847 928
Size: 971 1176 186 586 3751
Fragments arranged by size:
3751 1176 971 586 186
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4861 4895 1903
Size: 34 2924 34 3678
Fragments arranged by size:
3678 2924 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4562 5772 4562
Size: 1210 5460
BclI T'GATC_A
Cuts at: 1548 2614 1548
Size: 1066 5604
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2255 3708 5281
Size: 107 122 71 631 605 583 1453 1573
Cuts at: 5281 136
Size: 1525
Fragments arranged by size:
1573 1525 1453 631 605 583 122 107
71
BglII A'GATC_T
Cuts at: 3034 6665 3034
Size: 3631 3039
BlpI GC'TnA_GC
Cuts at: 4087 4087
Size: 6670
Bme1580I G_kGCm'C
Cuts at: 1790 3864 4731 5887 1790
Size: 2074 867 1156 2573
Fragments arranged by size:
2573 2074 1156 867
BmgBI CAC'GTC
Cuts at: 1318 3908 1318
Size: 2590 4080
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2814 3189 4401
Size: 416 53 83 399 24 1397 375 1212
Cuts at: 4401 5359 442
Size: 958 1753
Fragments arranged by size:
1753 1397 1212 958 416 399 375 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6670
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3749 3749
Size: 6670
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2871 3003 3552 3579 3628 5330 1239
Size: 1632 132 549 27 49 1702 2579
Fragments arranged by size:
2579 1702 1632 549 132 49 27
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2067 4795 5660 5958 2067
Size: 2728 865 298 2779
Fragments arranged by size:
2779 2728 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5333 882
Size: 617 3834 2219
Fragments arranged by size:
3834 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4461 493
Size: 711 3257 2702
Fragments arranged by size:
3257 2702 711
BsaBI GATnn'nnATC
Cuts at: 3039 3197 6664 3039
Size: 158 3467 3045
Fragments arranged by size:
3467 3045 158
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2009 4477 4859 5494
Size: 53 83 186 936 476 2468 382 635
Cuts at: 5494 275
Size: 1451
Fragments arranged by size:
2468 1451 936 635 476 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2861 2877 5103 5501 5775 5922 6333 2861
Size: 16 2226 398 274 147 411 3198
Fragments arranged by size:
3198 2226 411 398 274 147 16
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2548 2578 2872 2902 3393
Size: 30 165 30 1145 30 294 30 491
Cuts at: 3393 3423 3528 3558 1178
Size: 30 105 30 4290
Fragments arranged by size:
4290 1145 491 294 165 105 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2973 3489 3498 3549 3915 1186
Size: 1787 516 9 51 366 3941
Fragments arranged by size:
3941 1787 516 366 51 9
BseYI C'CCAG_C
Cuts at: 2073 2486 5873 6369 2073
Size: 413 3387 496 2374
Fragments arranged by size:
3387 2374 496 413
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2391 2544 1163
Size: 573 655 153 5289
Fragments arranged by size:
5289 655 573 153
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4120 4881 5909 1540
Size: 275 186 2119 761 1028 2301
Fragments arranged by size:
2301 2119 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3942 4731 4816 5887 3942
Size: 789 85 1071 4725
Fragments arranged by size:
4725 1071 789 85
BsmI GAATG_Cn'
Cuts at: 4181 4274 4181
Size: 93 6577
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3864 3942 3989 4731 4816 5887 1391
Size: 399 2074 78 47 742 85 1071 2174
Fragments arranged by size:
2174 2074 1071 742 399 85 78 47
BspEI T'CCGG_A
Cuts at: 2861 6333 2861
Size: 3472 3198
BspHI T'CATG_A
Cuts at: 4559 6289 4559
Size: 1730 4940
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4025 844
Size: 3181 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4557 1394
Size: 429 2734 3507
Fragments arranged by size:
3507 2734 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5165 5339 65
Size: 1183 3917 174 1396
Fragments arranged by size:
3917 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5314 5501 1700
Size: 110 3504 187 2869
Fragments arranged by size:
3504 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3094 96
Size: 2998 3672
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6670
BssSI C'ACGA_G
Cuts at: 4730 5742 4730
Size: 1012 5658
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2405 794
Size: 1611 5059
BstBI TT'CG_AA
Cuts at: 5474 5474
Size: 6670
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6670
BstXI CCAn_nnnn'nTGG
Cuts at: 2111 2282 2531 2827 2111
Size: 171 249 296 5954
Fragments arranged by size:
5954 296 249 171
BstYI r'GATC_y
Cuts at: 1972 2738 3034 3058 4000 4367 4752 4769 6221
Size: 766 296 24 942 367 385 17 1452
Cuts at: 6221 6307 6319 6665 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 942 766 385 367 346 296
86 24 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2013 3018 3279 3412 3450 3469
Size: 552 939 9 1005 261 133 38 19
Cuts at: 3469 3775 3799 513
Size: 306 24 3384
Fragments arranged by size:
3384 1005 939 552 306 261 133 38
24 19 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3249 487
Size: 683 56 2023 3908
Fragments arranged by size:
3908 2023 683 56
BtsI GCAGTG_nn'
Cuts at: 2175 2307 2403 2556 3155 3264 3575 4186 4980
Size: 132 96 153 599 109 311 611 794
Cuts at: 4980 5000 2175
Size: 20 3845
Fragments arranged by size:
3845 794 611 599 311 153 132 109
96 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6670
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6635
DraI TTT'AAA
Cuts at: 3974 3974
Size: 6670
DraIII CAC_nnn'GTG
Cuts at: 3383 3413 3383
Size: 30 6640
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3453 5677 809
Size: 507 2137 2224 1802
Fragments arranged by size:
2224 2137 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3772 3805 4117
Size: 54 1425 50 275 18 1942 33 312
Cuts at: 4117 5006 6635 8
Size: 889 1629 43
Fragments arranged by size:
1942 1629 1425 889 312 275 54 50
43 33 18
EagI C'GGCC_G
Cuts at: 1537 1812 4117 1537
Size: 275 2305 4090
Fragments arranged by size:
4090 2305 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2908 3718 3892 4600 1353
Size: 1555 810 174 708 3423
Fragments arranged by size:
3423 1555 810 708 174
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3660 5257 5631 5777 387
Size: 1064 138 2071 1597 374 146 1280
Fragments arranged by size:
2071 1597 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2871 3003 3552 3579 3628 3666 4733
Size: 393 1239 132 549 27 49 38 1067
Cuts at: 4733 5330 6117 6385 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1239 1067 787 597 549 393 268
132 49 38 27
EcoNI CCTnn'n_nnAGG
Cuts at: 3209 3498 3209
Size: 289 6381
EcoO109I rG'GnC_Cy
Cuts at: 3437 3446 3503 3634 3860 3861 3914 3437
Size: 9 57 131 226 1 53 6193
Fragments arranged by size:
6193 226 131 57 53 9 1
EcoRI G'AATT_C
Cuts at: 3979 4357 3979
Size: 378 6292
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3347 3347
Size: 6670
FspI TGC'GCA
Cuts at: 5176 5176
Size: 6670
HaeII r_GCGC'y
Cuts at: 1678 2982 5817 1678
Size: 1304 2835 2531
Fragments arranged by size:
2835 2531 1304
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3056 3088 3528 3560 5311
Size: 32 135 32 1484 32 440 32 1751
Cuts at: 5311 5343 5385 5417 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1751 1484 440 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2453 2687 4014 4260 669
Size: 988 281 515 234 1327 246 3079
Fragments arranged by size:
3079 1327 988 515 281 246 234
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6670
HpaI GTT'AAC
Cuts at: 4260 4260
Size: 6670
KpnI G_GTAC'C
Cuts at: 2531 3995 2531
Size: 1464 5206
MfeI C'AATT_G
Cuts at: 4269 4269
Size: 6670
MluI A'CGCG_T
Cuts at: 6546 6546
Size: 6670
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2267 2830 5784 5968 1305
Size: 962 563 2954 184 2007
Fragments arranged by size:
2954 2007 962 563 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4848
Fragments arranged by size:
4848 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4628 4987 5146 518
Size: 648 3462 359 159 2042
Fragments arranged by size:
3462 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 4086 4763 5911 6156 1606
Size: 2480 677 1148 245 2120
Fragments arranged by size:
2480 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 3018 3450 513
Size: 552 939 1014 432 3733
Fragments arranged by size:
3733 1014 939 552 432
NdeI CA'TA_TG
Cuts at: 387 2110 2356 387
Size: 1723 246 4701
Fragments arranged by size:
4701 1723 246
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6670
NotI GC'GGCC_GC
Cuts at: 4117 4117
Size: 6670
NspI r_CATG'y
Cuts at: 1132 1229 3400 3715 4028 5573 1132
Size: 97 2171 315 313 1545 2229
Fragments arranged by size:
2229 2171 1545 315 313 97
PasI CC'CwG_GG
Cuts at: 1348 3854 1348
Size: 2506 4164
PciI A'CATG_T
Cuts at: 5569 5569
Size: 6670
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2384 3413 1108
Size: 1276 1029 4365
Fragments arranged by size:
4365 1276 1029
PfoI T'CCnGG_A
Cuts at: 3421 3421
Size: 6670
PmeI GTTT'AAAC
Cuts at: 3974 3974
Size: 6670
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4739 4771 6252 6284 4739
Size: 32 1481 32 5125
Fragments arranged by size:
5125 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3437 3446 3503 3914 3437
Size: 9 57 411 6193
Fragments arranged by size:
6193 411 57 9
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6670
PsiI TTA'TAA
Cuts at: 4240 4240
Size: 6670
PspOMI G'GGCC_C
Cuts at: 3860 3860
Size: 6670
PstI C_TGCA'G
Cuts at: 830 2044 4022 830
Size: 1214 1978 3478
Fragments arranged by size:
3478 1978 1214
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6670
SalI G'TCGA_C
Cuts at: 1936 4012 1936
Size: 2076 4594
SanDI GG'GwC_CC
Cuts at: 3446 3446
Size: 6670
SbfI CC_TGCA'GG
Cuts at: 4022 4022
Size: 6670
ScaI AGT'ACT
Cuts at: 4918 6630 4918
Size: 1712 4958
SfcI C'TryA_G
Cuts at: 826 1045 2029 2040 2056 2313 2512 2743 3054
Size: 219 984 11 16 257 199 231 311
Cuts at: 3054 4018 5153 5834 6025 826
Size: 964 1135 681 191 1471
Fragments arranged by size:
1471 1135 984 964 681 311 257 231
219 199 191 16 11
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6670
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6670
SmaI CCC'GGG
Cuts at: 1872 3509 3678 3997 1872
Size: 1637 169 319 4545
Fragments arranged by size:
4545 1637 319 169
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2082 4774 5675 5937 820
Size: 197 926 139 2692 901 262 1553
Fragments arranged by size:
2692 1553 926 901 262 197 139
SnaBI TAC'GTA
Cuts at: 493 4461 493
Size: 3968 2702
SpeI A'CTAG_T
Cuts at: 152 2447 2606 2717 152
Size: 2295 159 111 4105
Fragments arranged by size:
4105 2295 159 111
SphI G_CATG'C
Cuts at: 1132 3400 3715 4028 1132
Size: 2268 315 313 3774
Fragments arranged by size:
3774 2268 315 313
SspI AAT'ATT
Cuts at: 5 52 4594 5
Size: 47 4542 2081
Fragments arranged by size:
4542 2081 47
StuI AGG'CCT
Cuts at: 1517 2333 1517
Size: 816 5854
StyI C'CwwG_G
Cuts at: 513 1065 2004 2491 3018 3270 3450 4109 513
Size: 552 939 487 527 252 180 659 3074
Fragments arranged by size:
3074 939 659 552 527 487 252 180
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2117 2342 2819 3277 3834 4697 4714
Size: 1255 537 225 477 458 557 863 17
Cuts at: 4714 4867 5052 5497 325
Size: 153 185 445 1498
Fragments arranged by size:
1498 1255 863 557 537 477 458 445
225 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3094 4916 6628
Size: 274 80 33 51 1443 1117 1822 1712
Cuts at: 6628 96
Size: 138
Fragments arranged by size:
1822 1712 1443 1117 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2548 2580 2872 2904 3393 3425 6630 6662 2548
Size: 32 292 32 489 32 3205 32 2556
Fragments arranged by size:
3205 2556 489 292 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6670
XbaI T'CTAG_A
Cuts at: 3121 4006 3121
Size: 885 5785
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3277 3457 3554 3277
Size: 180 97 6393
Fragments arranged by size:
6393 180 97
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6670
XmaI C'CCGG_G
Cuts at: 1870 3507 3676 3995 1870
Size: 1637 169 319 4545
Fragments arranged by size:
4545 1637 319 169
XmnI GAAnn'nnTTC
Cuts at: 4799 4799
Size: 6670
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4478 276
Size: 53 83 186 936 2944 2468
Fragments arranged by size:
2944 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI SalI SanDI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI BaeI BbeI BbvCI BfrBI
Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PmlI PspXI PsrI PvuII RsrII SacI SacII SapI
SexAI SfiI SfoI SrfI SwaI