Restriction map for
pFN21AB4495
(Circular) (Six-base) MAPSORT of: pFN21AB4495.seq Check: 1392 from: 1 to: 7594
pFN21AB4495 7594 bp
With 182 enzymes: SgfI *
February 27, 2009 14:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2827 2827
Size: 7594
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5404 278
Size: 53 83 186 936 3868 2468
Fragments arranged by size:
3868 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2148 4937 1937
Size: 211 2789 4594
Fragments arranged by size:
4594 2789 211
Acc65I G'GTAC_C
Cuts at: 4915 4915
Size: 7594
AclI AA'CG_TT
Cuts at: 1563 5721 6094 1563
Size: 4158 373 3063
Fragments arranged by size:
4158 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2246 2704 3716 4439 4511 4846 5657 7041
Size: 614 458 1012 723 72 335 811 1384
Cuts at: 7041 7309 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 1012 811 723 614 458 335
268 72
AfeI AGC'GCT
Cuts at: 2732 4571 4796 2732
Size: 1839 225 5530
Fragments arranged by size:
5530 1839 225
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7397
AflIII A'CryG_T
Cuts at: 1203 6493 7470 1203
Size: 5290 977 1327
Fragments arranged by size:
5290 1327 977
AgeI A'CCGG_T
Cuts at: 6425 6425
Size: 7594
AhdI GACnn_n'nnGTC
Cuts at: 6323 6323
Size: 7594
AleI CACnn'nnGTG
Cuts at: 1166 4217 1166
Size: 3051 4543
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3041 3516 4235 1184
Size: 1857 475 719 4543
Fragments arranged by size:
4543 1857 719 475
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2742 2774 2742
Size: 32 7562
AlwNI CAG_nnn'CTG
Cuts at: 3117 3250 4237 4308 4510 4753 5337 6909 3117
Size: 133 987 71 202 243 584 1572 3802
Fragments arranged by size:
3802 1572 987 584 243 202 133 71
ApaI G_GGCC'C
Cuts at: 4180 4202 4180
Size: 22 7572
ApaLI G'TGCA_C
Cuts at: 4145 5651 6807 4145
Size: 1506 1156 4932
Fragments arranged by size:
4932 1506 1156
ApoI r'AATT_y
Cuts at: 1493 4250 4903 5129 5281 7390 1493
Size: 2757 653 226 152 2109 1697
Fragments arranged by size:
2757 2109 1697 653 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6148 160
Size: 872 5116 1606
Fragments arranged by size:
5116 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7594
AvaI C'yCGr_G
Cuts at: 1870 1943 2429 2825 3029 4195 4202 4388 4919
Size: 73 486 396 204 1166 7 186 531
Cuts at: 4919 1870
Size: 4545
Fragments arranged by size:
4545 1166 531 486 396 204 186 73
7
BamHI G'GATC_C
Cuts at: 2019 4924 5291 2019
Size: 2905 367 4322
Fragments arranged by size:
4322 2905 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3541 4915 6370 618
Size: 325 217 627 1754 1374 1455 1842
Fragments arranged by size:
1842 1754 1455 1374 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3102 3345 3756 4180 4202 4389 4913 1391
Size: 1711 243 411 424 22 187 524 4072
Fragments arranged by size:
4072 1711 524 424 411 243 187 22
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 6623
BbvCI CC'TCA_GC
Cuts at: 3831 3831
Size: 7594
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2635 2669 5785 5819 1903
Size: 34 698 34 3116 34 3678
Fragments arranged by size:
3678 3116 698 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5486 6696 5486
Size: 1210 6384
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7594
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2701 3560
Size: 107 122 71 631 605 337 692 859
Cuts at: 3560 3865 6205 136
Size: 305 2340 1525
Fragments arranged by size:
2340 1525 859 692 631 605 337 305
122 107 71
BglII A'GATC_T
Cuts at: 3063 7589 3063
Size: 4526 3068
BlpI GC'TnA_GC
Cuts at: 2013 2623 3149 4061 4208 5011 2013
Size: 610 526 912 147 803 4596
Fragments arranged by size:
4596 912 803 610 526 147
Bme1580I G_kGCm'C
Cuts at: 1790 2532 3030 3505 4149 4180 4202 5655 6811
Size: 742 498 475 644 31 22 1453 1156
Cuts at: 6811 1790
Size: 2573
Fragments arranged by size:
2573 1453 1156 742 644 498 475 31
22
BmgBI CAC'GTC
Cuts at: 1318 3040 1318
Size: 1722 5872
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2127 3805 4821
Size: 416 53 83 399 24 710 1678 1016
Cuts at: 4821 5325 6283 442
Size: 504 958 1753
Fragments arranged by size:
1753 1678 1016 958 710 504 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7594
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2441 3116 3158 3179 3227 3359 4049 4247
Size: 1202 675 42 21 48 132 690 198
Cuts at: 4247 4729 6254 1239
Size: 482 1525 2579
Fragments arranged by size:
2579 1525 1202 690 675 482 198 132
48 42 21
Bpu10I CC'TnA_GC
Cuts at: 2081 3831 2081
Size: 1750 5844
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3599 3866 3956 3974 5719 6584 6882 3599
Size: 267 90 18 1745 865 298 4311
Fragments arranged by size:
4311 1745 865 298 267 90 18
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3047 3440 4609 6257 882
Size: 617 1548 393 1169 1648 2219
Fragments arranged by size:
2219 1648 1548 1169 617 393
BsaAI yAC'GTr
Cuts at: 493 1204 3025 3355 4260 4339 5385 493
Size: 711 1821 330 905 79 1046 2702
Fragments arranged by size:
2702 1821 1046 905 711 330 79
BsaBI GATnn'nnATC
Cuts at: 2189 7588 2189
Size: 5399 2195
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4326 4400 4623 5401
Size: 53 83 186 936 2793 74 223 778
Cuts at: 5401 5783 6418 275
Size: 382 635 1451
Fragments arranged by size:
2793 1451 936 778 635 382 223 186
83 74 53
BsaWI w'CCGG_w
Cuts at: 2346 6027 6425 6699 6846 7257 2346
Size: 3681 398 274 147 411 2683
Fragments arranged by size:
3681 2683 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2303 2333 1178
Size: 30 165 30 900 30 6439
Fragments arranged by size:
6439 900 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2311 3268 3819 4082 1186
Size: 1125 957 551 263 4698
Fragments arranged by size:
4698 1125 957 551 263
BseYI C'CCAG_C
Cuts at: 2540 3057 3700 4184 4566 6797 7293 2540
Size: 517 643 484 382 2231 496 2841
Fragments arranged by size:
2841 2231 643 517 496 484 382
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2351 3314 4739 1163
Size: 573 615 963 1425 4018
Fragments arranged by size:
4018 1425 963 615 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5044 5805 6833 1540
Size: 275 186 3043 761 1028 2301
Fragments arranged by size:
3043 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3102 3756 4149 4196 4214 4542 5655 5740 6811
Size: 654 393 47 18 328 1113 85 1071
Cuts at: 6811 3102
Size: 3885
Fragments arranged by size:
3885 1113 1071 654 393 328 85 47
18
BsmI GAATG_Cn'
Cuts at: 5105 5198 5105
Size: 93 7501
BsmBI CGTCTCn'nnnn_
Cuts at: 2093 2093
Size: 7594
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2532 3030 3102 3345 3505 3756 4149
Size: 399 742 498 72 243 160 251 393
Cuts at: 4149 4180 4196 4202 4214 4389 4542 4913 5655
Size: 31 16 6 12 175 153 371 742
Cuts at: 5655 5740 6811 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 742 498 399 393 371
251 243 175 160 153 85 72 31
16 12 6
BspEI T'CCGG_A
Cuts at: 7257 7257
Size: 7594
BspHI T'CATG_A
Cuts at: 5483 7213 5483
Size: 1730 5864
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2585 2827 4032 4353 4949 844
Size: 1741 242 1205 321 596 3489
Fragments arranged by size:
3489 1741 1205 596 321 242
BsrBI CCG'CTC
Cuts at: 1394 1823 2027 3292 4072 4351 4393 5481 1394
Size: 429 204 1265 780 279 42 1088 3507
Fragments arranged by size:
3507 1265 1088 780 429 279 204 42
BsrDI GCAATG_nn'
Cuts at: 65 1248 6089 6263 65
Size: 1183 4841 174 1396
Fragments arranged by size:
4841 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2695 2804 6238 6425 1700
Size: 110 885 109 3434 187 2869
Fragments arranged by size:
3434 2869 885 187 110 109
BsrGI T'GTAC_A
Cuts at: 96 2469 96
Size: 2373 5221
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7594
BssSI C'ACGA_G
Cuts at: 4142 5654 6666 4142
Size: 1512 1012 5070
Fragments arranged by size:
5070 1512 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4237 794
Size: 3443 4151
BstBI TT'CG_AA
Cuts at: 2854 6398 2854
Size: 3544 4050
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7594
BstXI CCAn_nnnn'nTGG
Cuts at: 2305 2419 2924 2305
Size: 114 505 6975
Fragments arranged by size:
6975 505 114
BstYI r'GATC_y
Cuts at: 1972 2019 3063 3610 4233 4924 5291 5676 5693
Size: 47 1044 547 623 691 367 385 17
Cuts at: 5693 7145 7231 7243 7589 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1044 691 623 547 385 367
346 86 47 17 12
Bsu36I CC'TnA_GG
Cuts at: 3251 3251
Size: 7594
BtgI C'CryG_G
Cuts at: 513 1065 2004 2946 3370 4548 513
Size: 552 939 942 424 1178 3559
Fragments arranged by size:
3559 1178 942 939 552 424
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6855
Fragments arranged by size:
6855 683 56
BtsI GCAGTG_nn'
Cuts at: 5110 5904 5924 5110
Size: 794 20 6780
Fragments arranged by size:
6780 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7594
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7559
DraI TTT'AAA
Cuts at: 4898 4898
Size: 7594
DraIII CAC_nnn'GTG
Cuts at: 2363 3402 2363
Size: 1039 6555
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6601 809
Size: 507 5285 1802
Fragments arranged by size:
5285 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2028 2914 4596
Size: 54 1425 50 275 18 198 886 1682
Cuts at: 4596 5041 5930 7559 8
Size: 445 889 1629 43
Fragments arranged by size:
1682 1629 1425 889 886 445 275 198
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5041 1537
Size: 275 3229 4090
Fragments arranged by size:
4090 3229 275
EarI CTCTTCn'nnn_
Cuts at: 1353 5524 1353
Size: 4171 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6181 6555 6701 387
Size: 1064 138 4592 374 146 1280
Fragments arranged by size:
4592 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3100 3754 3100
Size: 654 6940
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2246 2441 2704 3116 3158 3179 3227
Size: 393 614 195 263 412 42 21 48
Cuts at: 3227 3359 3716 4049 4247 4439 4511 4729 4846
Size: 132 357 333 198 192 72 218 117
Cuts at: 4846 5657 6254 7041 7309 1239
Size: 811 597 787 268 1524
Fragments arranged by size:
1524 811 787 614 597 412 393 357
333 268 263 218 198 195 192 132
117 72 48 42 21
EcoNI CCTnn'n_nnAGG
Cuts at: 4276 4276
Size: 7594
EcoO109I rG'GnC_Cy
Cuts at: 2067 3114 3603 4176 4199 4281 4507 2067
Size: 1047 489 573 23 82 226 5154
Fragments arranged by size:
5154 1047 573 489 226 82 23
EcoRI G'AATT_C
Cuts at: 4903 5281 4903
Size: 378 7216
FspI TGC'GCA
Cuts at: 6100 6100
Size: 7594
HaeII r_GCGC'y
Cuts at: 1678 2734 4315 4573 4798 6741 1678
Size: 1056 1581 258 225 1943 2531
Fragments arranged by size:
2531 1943 1581 1056 258 225
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2368 2400 6235 6267 6309
Size: 32 135 32 796 32 3835 32 42
Cuts at: 6309 6341 1373
Size: 32 2626
Fragments arranged by size:
3835 2626 796 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4938 5184 669
Size: 988 281 3000 246 3079
Fragments arranged by size:
3079 3000 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7594
HpaI GTT'AAC
Cuts at: 5184 5184
Size: 7594
KpnI G_GTAC'C
Cuts at: 4919 4919
Size: 7594
MfeI C'AATT_G
Cuts at: 5193 5193
Size: 7594
MluI A'CGCG_T
Cuts at: 7470 7470
Size: 7594
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6708 6892 1305
Size: 5403 184 2007
Fragments arranged by size:
5403 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2916 4598 10
Size: 54 1425 343 1084 1682 3006
Fragments arranged by size:
3006 1682 1425 1084 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2120 2303 3023 3498 4217 5552 5911
Size: 648 954 183 720 475 719 1335 359
Cuts at: 5911 6070 518
Size: 159 2042
Fragments arranged by size:
2042 1335 954 720 719 648 475 359
183 159
MspA1I CmG'CkG
Cuts at: 1606 2017 2370 3094 3303 3328 3571 3694 4093
Size: 411 353 724 209 25 243 123 399
Cuts at: 4093 4308 5010 5687 6835 7080 1606
Size: 215 702 677 1148 245 2120
Fragments arranged by size:
2120 1148 724 702 677 411 399 353
245 243 215 209 123 25
NcoI C'CATG_G
Cuts at: 513 1065 2004 3370 513
Size: 552 939 1366 4737
Fragments arranged by size:
4737 1366 939 552
NdeI CA'TA_TG
Cuts at: 387 3277 387
Size: 2890 4704
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7594
NotI GC'GGCC_GC
Cuts at: 5041 5041
Size: 7594
NspI r_CATG'y
Cuts at: 1132 1229 4952 6497 1132
Size: 97 3723 1545 2229
Fragments arranged by size:
3723 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 3047 4330 1348
Size: 1699 1283 4612
Fragments arranged by size:
4612 1699 1283
PciI A'CATG_T
Cuts at: 6493 6493
Size: 7594
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2902 2947 3402 4107 4510 4797 1108
Size: 1794 45 455 705 403 287 3905
Fragments arranged by size:
3905 1794 705 455 403 287 45
PfoI T'CCnGG_A
Cuts at: 3948 4527 4746 3948
Size: 579 219 6796
Fragments arranged by size:
6796 579 219
PmeI GTTT'AAAC
Cuts at: 4898 4898
Size: 7594
PmlI CAC'GTG
Cuts at: 3025 3025
Size: 7594
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5663 5695 7176 7208 5663
Size: 32 1481 32 6049
Fragments arranged by size:
6049 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2067 3114 4281 2067
Size: 1047 1167 5380
Fragments arranged by size:
5380 1167 1047
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7594
PsiI TTA'TAA
Cuts at: 5164 5164
Size: 7594
PspOMI G'GGCC_C
Cuts at: 4176 4198 4176
Size: 22 7572
PstI C_TGCA'G
Cuts at: 830 3122 3302 3570 4946 830
Size: 2292 180 268 1376 3478
Fragments arranged by size:
3478 2292 1376 268 180
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7594
PvuII CAG'CTG
Cuts at: 3303 3328 3571 3694 4093 4308 3303
Size: 25 243 123 399 215 6589
Fragments arranged by size:
6589 399 243 215 123 25
SacI G_AGCT'C
Cuts at: 3102 3756 3102
Size: 654 6940
SalI G'TCGA_C
Cuts at: 1936 4936 1936
Size: 3000 4594
SanDI GG'GwC_CC
Cuts at: 2067 2067
Size: 7594
SbfI CC_TGCA'GG
Cuts at: 4946 4946
Size: 7594
ScaI AGT'ACT
Cuts at: 2569 5842 7554 2569
Size: 3273 1712 2609
Fragments arranged by size:
3273 2609 1712
SfcI C'TryA_G
Cuts at: 826 1045 2737 3118 3298 3566 4554 4942 6077
Size: 219 1692 381 180 268 988 388 1135
Cuts at: 6077 6758 6949 826
Size: 681 191 1471
Fragments arranged by size:
1692 1471 1135 988 681 388 381 268
219 191 180
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7594
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7594
SmaI CCC'GGG
Cuts at: 1872 4921 1872
Size: 3049 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3614 3881 3971 3989 5698 6599
Size: 197 926 1671 267 90 18 1709 901
Cuts at: 6599 6861 820
Size: 262 1553
Fragments arranged by size:
1709 1671 1553 926 901 267 262 197
90 18
SnaBI TAC'GTA
Cuts at: 493 5385 493
Size: 4892 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7594
SphI G_CATG'C
Cuts at: 1132 4952 1132
Size: 3820 3774
SspI AAT'ATT
Cuts at: 5 52 5518 5
Size: 47 5466 2081
Fragments arranged by size:
5466 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7594
StyI C'CwwG_G
Cuts at: 513 1065 2004 2235 2412 3370 4101 4599 5033
Size: 552 939 231 177 958 731 498 434
Cuts at: 5033 513
Size: 3074
Fragments arranged by size:
3074 958 939 731 552 498 434 231
177
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3474 3559 3824 5621 5638 5791 5976
Size: 1255 1894 85 265 1797 17 153 185
Cuts at: 5976 6421 325
Size: 445 1498
Fragments arranged by size:
1894 1797 1498 1255 445 265 185 153
85 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2469 2567 4032
Size: 274 80 33 51 1443 492 98 1465
Cuts at: 4032 5840 7552 96
Size: 1808 1712 138
Fragments arranged by size:
1808 1712 1465 1443 492 274 138 98
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7554 7586 7554
Size: 32 7562
Tth111I GACn'n_nGTC
Cuts at: 1316 3314 1316
Size: 1998 5596
XbaI T'CTAG_A
Cuts at: 4930 4930
Size: 7594
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2569 3044 4159 4615 2569
Size: 475 1115 456 5548
Fragments arranged by size:
5548 1115 475 456
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7594
XmaI C'CCGG_G
Cuts at: 1870 4919 1870
Size: 3049 4545
XmnI GAAnn'nnTTC
Cuts at: 4460 5723 4460
Size: 1263 6331
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5402 276
Size: 53 83 186 936 3868 2468
Fragments arranged by size:
3868 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI BbeI BfrBI BplI BsiWI BstZ17I
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PspXI PsrI RsrII SacII SapI
SexAI SfiI SfoI SrfI SwaI