Restriction map for
pFN21AB4483
(Circular) (Six-base) MAPSORT of: pFN21AB4483.seq Check: 1474 from: 1 to: 6304
pFN21AB4483 6304 bp
With 182 enzymes: SgfI *
June 25, 2013 12:09 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3441 4114 278
Size: 53 83 186 936 1905 673 2468
Fragments arranged by size:
2468 1905 936 673 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2529 3647 1937
Size: 592 1118 4594
Fragments arranged by size:
4594 1118 592
Acc65I G'GTAC_C
Cuts at: 3513 3625 3513
Size: 112 6192
AclI AA'CG_TT
Cuts at: 1563 4431 4804 1563
Size: 2868 373 3063
Fragments arranged by size:
3063 2868 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2489 3485 4367 5751 6019 1632
Size: 857 996 882 1384 268 1917
Fragments arranged by size:
1917 1384 996 882 857 268
AfeI AGC'GCT
Cuts at: 2833 2833
Size: 6304
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6107
AflIII A'CryG_T
Cuts at: 1203 5203 6180 1203
Size: 4000 977 1327
Fragments arranged by size:
4000 1327 977
AgeI A'CCGG_T
Cuts at: 5135 5135
Size: 6304
AhdI GACnn_n'nnGTC
Cuts at: 5033 5033
Size: 6304
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6304
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2504 1184
Size: 1320 4984
AlwNI CAG_nnn'CTG
Cuts at: 2516 3202 4047 5619 2516
Size: 686 845 1572 3201
Fragments arranged by size:
3201 1572 845 686
ApaI G_GGCC'C
Cuts at: 2026 2026
Size: 6304
ApaLI G'TGCA_C
Cuts at: 4361 5517 4361
Size: 1156 5148
ApoI r'AATT_y
Cuts at: 1493 3389 3613 3839 3991 6100 1493
Size: 1896 224 226 152 2109 1697
Fragments arranged by size:
2109 1896 1697 226 224 152
AseI AT'TA_AT
Cuts at: 160 1032 4858 160
Size: 872 3826 1606
Fragments arranged by size:
3826 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6304
AvaI C'yCGr_G
Cuts at: 1870 1943 2085 2150 3230 3629 1870
Size: 73 142 65 1080 399 4545
Fragments arranged by size:
4545 1080 399 142 73 65
AvrII C'CTAG_G
Cuts at: 2554 2554
Size: 6304
BamHI G'GATC_C
Cuts at: 3634 4001 3634
Size: 367 5937
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2125 2488 2558 3218 3513
Size: 325 217 627 338 363 70 660 295
Cuts at: 3513 3625 5080 618
Size: 112 1455 1842
Fragments arranged by size:
1842 1455 660 627 363 338 325 295
217 112 70
BanII G_rGCy'C
Cuts at: 1391 2026 2070 2178 2818 2887 3216 3273 3623
Size: 635 44 108 640 69 329 57 350
Cuts at: 3623 1391
Size: 4072
Fragments arranged by size:
4072 640 635 350 329 108 69 57
44
BbeI G_GCGC'C
Cuts at: 2129 3222 2129
Size: 1093 5211
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3076 928
Size: 971 1177 4156
Fragments arranged by size:
4156 1177 971
BbvCI CC'TCA_GC
Cuts at: 2336 3368 2336
Size: 1032 5272
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4495 4529 1903
Size: 34 2558 34 3678
Fragments arranged by size:
3678 2558 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2909 4196 5406 2909
Size: 1287 1210 3807
Fragments arranged by size:
3807 1287 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6304
BfrBI ATG'CAT
Cuts at: 3455 3455
Size: 6304
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4915 136
Size: 107 122 71 631 605 3243 1525
Fragments arranged by size:
3243 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2161 2617 3168 6299 2161
Size: 456 551 3131 2166
Fragments arranged by size:
3131 2166 551 456
BlpI GC'TnA_GC
Cuts at: 2498 3203 3721 2498
Size: 705 518 5081
Fragments arranged by size:
5081 705 518
Bme1580I G_kGCm'C
Cuts at: 1790 2026 4365 5521 1790
Size: 236 2339 1156 2573
Fragments arranged by size:
2573 2339 1156 236
BmgBI CAC'GTC
Cuts at: 1318 2569 3121 1318
Size: 1251 552 4501
Fragments arranged by size:
4501 1251 552
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2812 4035 4993
Size: 416 53 83 399 24 1395 1223 958
Cuts at: 4993 442
Size: 1753
Fragments arranged by size:
1753 1395 1223 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6304
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2612 2612
Size: 6304
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2423 2607 2622 3125 3146 4964 1239
Size: 1184 184 15 503 21 1818 2579
Fragments arranged by size:
2579 1818 1184 503 184 21 15
Bpu10I CC'TnA_GC
Cuts at: 2172 2336 3368 2172
Size: 164 1032 5108
Fragments arranged by size:
5108 1032 164
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3024 4429 5294 5592 3024
Size: 1405 865 298 3736
Fragments arranged by size:
3736 1405 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2805 4967 882
Size: 617 1306 2162 2219
Fragments arranged by size:
2219 2162 1306 617
BsaAI yAC'GTr
Cuts at: 493 1204 2977 3058 4095 493
Size: 711 1773 81 1037 2702
Fragments arranged by size:
2702 1773 1037 711 81
BsaBI GATnn'nnATC
Cuts at: 6298 6298
Size: 6304
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2126 3134 3219 3438
Size: 53 83 186 936 593 1008 85 219
Cuts at: 3438 4111 4493 5128 275
Size: 673 382 635 1451
Fragments arranged by size:
1451 1008 936 673 635 593 382 219
186 85 83 53
BsaWI w'CCGG_w
Cuts at: 2718 4737 5135 5409 5556 5967 2718
Size: 2019 398 274 147 411 3055
Fragments arranged by size:
3055 2019 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6079
Fragments arranged by size:
6079 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2401 1186
Size: 1215 5089
BseYI C'CCAG_C
Cuts at: 2011 2062 2398 5507 6003 2011
Size: 51 336 3109 496 2312
Fragments arranged by size:
3109 2312 496 336 51
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5731
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3754 4515 5543 1540
Size: 275 186 1753 761 1028 2301
Fragments arranged by size:
2301 1753 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2084 3273 4365 4450 5521 2084
Size: 1189 1092 85 1071 2867
Fragments arranged by size:
2867 1189 1092 1071 85
BsmI GAATG_Cn'
Cuts at: 3815 3908 3815
Size: 93 6211
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2026 2070 2084 2178 2818 2887 3216
Size: 399 236 44 14 94 640 69 329
Cuts at: 3216 3273 3623 4365 4450 5521 1391
Size: 57 350 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 640 399 350 329 236
94 85 69 57 44 14
BspEI T'CCGG_A
Cuts at: 5967 5967
Size: 6304
BspHI T'CATG_A
Cuts at: 4193 5923 4193
Size: 1730 4574
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3659 844
Size: 2815 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2437 3130 4191 1394
Size: 429 614 693 1061 3507
Fragments arranged by size:
3507 1061 693 614 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4799 4973 65
Size: 1183 3551 174 1396
Fragments arranged by size:
3551 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3558 3579 4948 5135 1700
Size: 110 1748 21 1369 187 2869
Fragments arranged by size:
2869 1748 1369 187 110 21
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6304
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6304
BssSI C'ACGA_G
Cuts at: 3266 4364 5376 3266
Size: 1098 1012 4194
Fragments arranged by size:
4194 1098 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6304
BstBI TT'CG_AA
Cuts at: 5108 5108
Size: 6304
BstEII G'GTnAC_C
Cuts at: 1184 2920 3295 1184
Size: 1736 375 4193
Fragments arranged by size:
4193 1736 375
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2011
Size: 6304
BstYI r'GATC_y
Cuts at: 1972 2161 2617 3168 3634 4001 4386 4403 5855
Size: 189 456 551 466 367 385 17 1452
Cuts at: 5855 5941 5953 6299 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 551 466 456 385 367 346
189 86 17 12
BstZ17I GTA'TAC
Cuts at: 2530 2530
Size: 6304
Bsu36I CC'TnA_GG
Cuts at: 2474 2474
Size: 6304
BtgI C'CryG_G
Cuts at: 513 1065 2120 2925 513
Size: 552 1055 805 3892
Fragments arranged by size:
3892 1055 805 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2917 487
Size: 683 56 1691 3874
Fragments arranged by size:
3874 1691 683 56
BtsI GCAGTG_nn'
Cuts at: 3593 3820 4614 4634 3593
Size: 227 794 20 5263
Fragments arranged by size:
5263 794 227 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6304
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6269
DraI TTT'AAA
Cuts at: 3608 3608
Size: 6304
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3080 5311 809
Size: 507 1764 2231 1802
Fragments arranged by size:
2231 1802 1764 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3030 3549 3751
Size: 54 1425 50 275 18 1200 519 202
Cuts at: 3751 4640 6269 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1200 889 519 275 202 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3751 1537
Size: 275 1939 4090
Fragments arranged by size:
4090 1939 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3572 4234 1353
Size: 2219 662 3423
Fragments arranged by size:
3423 2219 662
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2891 4891 5265 5411 387
Size: 1064 138 1302 2000 374 146 1280
Fragments arranged by size:
2000 1302 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3271 3271
Size: 6304
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2423 2489 2607 2622 3125 3146 3485
Size: 393 791 66 118 15 503 21 339
Cuts at: 3485 4367 4964 5751 6019 1239
Size: 882 597 787 268 1524
Fragments arranged by size:
1524 882 791 787 597 503 393 339
268 118 66 21 15
EcoNI CCTnn'n_nnAGG
Cuts at: 2312 2312
Size: 6304
EcoO109I rG'GnC_Cy
Cuts at: 2023 2266 2023
Size: 243 6061
EcoRI G'AATT_C
Cuts at: 3389 3613 3991 3389
Size: 224 378 5702
Fragments arranged by size:
5702 378 224
FspI TGC'GCA
Cuts at: 4810 4810
Size: 6304
HaeII r_GCGC'y
Cuts at: 1678 2129 2835 3222 5451 1678
Size: 451 706 387 2229 2531
Fragments arranged by size:
2531 2229 706 451 387
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3440 3472 4945 4977 5019
Size: 32 135 32 1868 32 1473 32 42
Cuts at: 5019 5051 1373
Size: 32 2626
Fragments arranged by size:
2626 1868 1473 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3313 3648 3894 669
Size: 988 281 1375 335 246 3079
Fragments arranged by size:
3079 1375 988 335 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6304
HpaI GTT'AAC
Cuts at: 3313 3894 3313
Size: 581 5723
KasI G'GCGC_C
Cuts at: 2125 3218 2125
Size: 1093 5211
KpnI G_GTAC'C
Cuts at: 3517 3629 3517
Size: 112 6192
MfeI C'AATT_G
Cuts at: 3903 3903
Size: 6304
MluI A'CGCG_T
Cuts at: 6180 6180
Size: 6304
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3459 5418 5602 1305
Size: 2154 1959 184 2007
Fragments arranged by size:
2154 2007 1959 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3551 10
Size: 54 1425 343 1719 2763
Fragments arranged by size:
2763 1719 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2486 4262 4621 4780 518
Size: 648 1320 1776 359 159 2042
Fragments arranged by size:
2042 1776 1320 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2402 2410 3139 3199 3720 4397 5545 5790
Size: 796 8 729 60 521 677 1148 245
Cuts at: 5790 1606
Size: 2120
Fragments arranged by size:
2120 1148 796 729 677 521 245 60
8
NarI GG'CG_CC
Cuts at: 2126 3219 2126
Size: 1093 5211
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5752
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6304
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6304
NotI GC'GGCC_GC
Cuts at: 3751 3751
Size: 6304
NsiI A_TGCA'T
Cuts at: 3457 3457
Size: 6304
NspI r_CATG'y
Cuts at: 1132 1229 3662 5207 1132
Size: 97 2433 1545 2229
Fragments arranged by size:
2433 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2636 2809 1348
Size: 1288 173 4843
Fragments arranged by size:
4843 1288 173
PciI A'CATG_T
Cuts at: 5203 5203
Size: 6304
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6304
PmeI GTTT'AAAC
Cuts at: 3608 3608
Size: 6304
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2351 2383 2973 3005 4373 4405 5886 5918 2351
Size: 32 590 32 1368 32 1481 32 2737
Fragments arranged by size:
2737 1481 1368 590 32 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6304
PsiI TTA'TAA
Cuts at: 3874 3874
Size: 6304
PspOMI G'GGCC_C
Cuts at: 2022 2022
Size: 6304
PstI C_TGCA'G
Cuts at: 830 2246 2332 3588 3656 830
Size: 1416 86 1256 68 3478
Fragments arranged by size:
3478 1416 1256 86 68
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6304
PvuII CAG'CTG
Cuts at: 2402 2410 3199 2402
Size: 8 789 5507
Fragments arranged by size:
5507 789 8
SacI G_AGCT'C
Cuts at: 3273 3273
Size: 6304
SalI G'TCGA_C
Cuts at: 1936 3646 1936
Size: 1710 4594
SapI GCTCTTCn'nnn_
Cuts at: 3572 3572
Size: 6304
SbfI CC_TGCA'GG
Cuts at: 3656 3656
Size: 6304
ScaI AGT'ACT
Cuts at: 4552 6264 4552
Size: 1712 4592
SexAI A'CCwGG_T
Cuts at: 2532 3144 2532
Size: 612 5692
SfcI C'TryA_G
Cuts at: 826 1045 2242 2328 2450 3584 3652 4787 5468
Size: 219 1197 86 122 1134 68 1135 681
Cuts at: 5468 5659 826
Size: 191 1471
Fragments arranged by size:
1471 1197 1135 1134 681 219 191 122
86 68
SfoI GGC'GCC
Cuts at: 2127 3220 2127
Size: 1093 5211
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6304
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6304
SmaI CCC'GGG
Cuts at: 1872 2087 3631 1872
Size: 215 1544 4545
Fragments arranged by size:
4545 1544 215
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3039 4408 5309 5571 820
Size: 197 926 1096 1369 901 262 1553
Fragments arranged by size:
1553 1369 1096 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4095 493
Size: 3602 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6304
SphI G_CATG'C
Cuts at: 1132 3662 1132
Size: 2530 3774
SspI AAT'ATT
Cuts at: 5 52 4228 5
Size: 47 4176 2081
Fragments arranged by size:
4176 2081 47
StuI AGG'CCT
Cuts at: 1517 2318 2375 3228 1517
Size: 801 57 853 4593
Fragments arranged by size:
4593 853 801 57
StyI C'CwwG_G
Cuts at: 513 1065 2313 2554 3473 3743 513
Size: 552 1248 241 919 270 3074
Fragments arranged by size:
3074 1248 919 552 270 241
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3055 4331 4348 4501 4686 5131 325
Size: 1255 1475 1276 17 153 185 445 1498
Fragments arranged by size:
1498 1475 1276 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2820 4550 6262
Size: 274 80 33 51 1443 843 1730 1712
Cuts at: 6262 96
Size: 138
Fragments arranged by size:
1730 1712 1443 843 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6264 6296 6264
Size: 32 6272
Tth111I GACn'n_nGTC
Cuts at: 1316 2714 3080 1316
Size: 1398 366 4540
Fragments arranged by size:
4540 1398 366
XbaI T'CTAG_A
Cuts at: 3640 3640
Size: 6304
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6304
XmaI C'CCGG_G
Cuts at: 1870 2085 3629 1870
Size: 215 1544 4545
Fragments arranged by size:
4545 1544 215
XmnI GAAnn'nnTTC
Cuts at: 4433 4433
Size: 6304
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3439 4112 276
Size: 53 83 186 936 1905 673 2468
Fragments arranged by size:
2468 1905 936 673 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NarI NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AloI AscI BaeI BsiWI BsmBI DraIII EcoRV
FalI FseI FspAI NaeI NgoMIV NruI PacI PfoI
PmlI PpuMI PspXI PsrI RsrII SacII SanDI SfiI
SrfI SwaI XcmI