Restriction map for
pFN21AB4480
(Circular) (Six-base) MAPSORT of: pFN21AB4480.seq Check: 4791 from: 1 to: 5782
pFN21AB4480 5782 bp
With 182 enzymes: SgfI *
October 20, 2009 10:59 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3592 278
Size: 53 83 186 936 2056 2468
Fragments arranged by size:
2468 2056 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2858 3125 1937
Size: 921 267 4594
Fragments arranged by size:
4594 921 267
Acc65I G'GTAC_C
Cuts at: 3103 3103
Size: 5782
AclI AA'CG_TT
Cuts at: 1563 3909 4282 1563
Size: 2346 373 3063
Fragments arranged by size:
3063 2346 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2412 3845 5229 5497 1632
Size: 780 1433 1384 268 1917
Fragments arranged by size:
1917 1433 1384 780 268
AflII C'TTAA_G
Cuts at: 820 1017 2738 820
Size: 197 1721 3864
Fragments arranged by size:
3864 1721 197
AflIII A'CryG_T
Cuts at: 1203 4681 5658 1203
Size: 3478 977 1327
Fragments arranged by size:
3478 1327 977
AgeI A'CCGG_T
Cuts at: 4613 4613
Size: 5782
AhdI GACnn_n'nnGTC
Cuts at: 4511 4511
Size: 5782
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5782
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5782
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2752 2784 2752
Size: 32 5750
AlwNI CAG_nnn'CTG
Cuts at: 3525 5097 3525
Size: 1572 4210
ApaLI G'TGCA_C
Cuts at: 3839 4995 3839
Size: 1156 4626
ApoI r'AATT_y
Cuts at: 1493 2443 2633 2886 3011 3091 3317 3469 5578
Size: 950 190 253 125 80 226 152 2109
Cuts at: 5578 1493
Size: 1697
Fragments arranged by size:
2109 1697 950 253 226 190 152 125
80
AseI AT'TA_AT
Cuts at: 160 1032 2732 4336 160
Size: 872 1700 1604 1606
Fragments arranged by size:
1700 1606 1604 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5782
AvaI C'yCGr_G
Cuts at: 1870 1943 3107 1870
Size: 73 1164 4545
Fragments arranged by size:
4545 1164 73
BamHI G'GATC_C
Cuts at: 3112 3479 3112
Size: 367 5415
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2346 3103 4558 618
Size: 325 217 627 559 757 1455 1842
Fragments arranged by size:
1842 1455 757 627 559 325 217
BanII G_rGCy'C
Cuts at: 1391 3049 3101 1391
Size: 1658 52 4072
Fragments arranged by size:
4072 1658 52
BbeI G_GCGC'C
Cuts at: 2350 2350
Size: 5782
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2840 928
Size: 971 941 3870
Fragments arranged by size:
3870 971 941
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3973 4007 1903
Size: 34 50 34 1952 34 3678
Fragments arranged by size:
3678 1952 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3674 4884 3674
Size: 1210 4572
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5782
BfrBI ATG'CAT
Cuts at: 2729 2729
Size: 5782
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4393 136
Size: 107 122 71 631 605 2721 1525
Fragments arranged by size:
2721 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5777 5777
Size: 5782
BlpI GC'TnA_GC
Cuts at: 3199 3199
Size: 5782
Bme1580I G_kGCm'C
Cuts at: 1790 3843 4999 1790
Size: 2053 1156 2573
Fragments arranged by size:
2573 2053 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5782
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3513 4471 442
Size: 416 53 83 399 24 2096 958 1753
Fragments arranged by size:
2096 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5782
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4442 1239
Size: 3203 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3907 4772 5070 3907
Size: 865 298 4619
Fragments arranged by size:
4619 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4445 882
Size: 617 2946 2219
Fragments arranged by size:
2946 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2713 3573 493
Size: 711 1509 860 2702
Fragments arranged by size:
2702 1509 860 711
BsaBI GATnn'nnATC
Cuts at: 5776 5776
Size: 5782
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2347 3589 3971 4606
Size: 53 83 186 936 814 1242 382 635
Cuts at: 4606 275
Size: 1451
Fragments arranged by size:
1451 1242 936 814 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4215 4613 4887 5034 5445 4215
Size: 398 274 147 411 4552
Fragments arranged by size:
4552 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2367 2397 1178
Size: 30 165 30 964 30 4563
Fragments arranged by size:
4563 964 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2105 2715 3057 1186
Size: 919 610 342 3911
Fragments arranged by size:
3911 919 610 342
BseYI C'CCAG_C
Cuts at: 2837 4985 5481 2837
Size: 2148 496 3138
Fragments arranged by size:
3138 2148 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2020 2451 1163
Size: 573 284 431 4494
Fragments arranged by size:
4494 573 431 284
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3232 3993 5021 1540
Size: 275 186 1231 761 1028 2301
Fragments arranged by size:
2301 1231 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2097 3049 3843 3928 4999 2097
Size: 952 794 85 1071 2880
Fragments arranged by size:
2880 1071 952 794 85
BsmI GAATG_Cn'
Cuts at: 2451 3006 3293 3386 2451
Size: 555 287 93 4847
Fragments arranged by size:
4847 555 287 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2096 2096
Size: 5782
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2097 3049 3101 3843 3928 4999 1391
Size: 399 307 952 52 742 85 1071 2174
Fragments arranged by size:
2174 1071 952 742 399 307 85 52
BspEI T'CCGG_A
Cuts at: 5445 5445
Size: 5782
BspHI T'CATG_A
Cuts at: 2368 3671 5401 2368
Size: 1303 1730 2749
Fragments arranged by size:
2749 1730 1303
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2406 3137 844
Size: 1562 731 3489
Fragments arranged by size:
3489 1562 731
BsrBI CCG'CTC
Cuts at: 1394 1823 3669 1394
Size: 429 1846 3507
Fragments arranged by size:
3507 1846 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4277 4451 65
Size: 1183 3029 174 1396
Fragments arranged by size:
3029 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4426 4613 1700
Size: 110 2616 187 2869
Fragments arranged by size:
2869 2616 187 110
BsrGI T'GTAC_A
Cuts at: 96 2587 96
Size: 2491 3291
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5782
BssSI C'ACGA_G
Cuts at: 3842 4854 3842
Size: 1012 4770
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5782
BstBI TT'CG_AA
Cuts at: 2173 2611 4586 2173
Size: 438 1975 3369
Fragments arranged by size:
3369 1975 438
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5782
BstYI r'GATC_y
Cuts at: 1972 3112 3479 3864 3881 5333 5419 5431 5777
Size: 1140 367 385 17 1452 86 12 346
Cuts at: 5777 1972
Size: 1977
Fragments arranged by size:
1977 1452 1140 385 367 346 86 17
12
BstZ17I GTA'TAC
Cuts at: 2859 2859
Size: 5782
Bsu36I CC'TnA_GG
Cuts at: 2916 2916
Size: 5782
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5230
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5043
Fragments arranged by size:
5043 683 56
BtsI GCAGTG_nn'
Cuts at: 3298 4092 4112 3298
Size: 794 20 4968
Fragments arranged by size:
4968 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5782
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5747
DraI TTT'AAA
Cuts at: 2925 3086 2925
Size: 161 5621
DraIII CAC_nnn'GTG
Cuts at: 2335 2335
Size: 5782
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4789 809
Size: 507 3473 1802
Fragments arranged by size:
3473 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3229 4118 5747
Size: 54 1425 50 275 18 1399 889 1629
Cuts at: 5747 8
Size: 43
Fragments arranged by size:
1629 1425 1399 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3229 1537
Size: 275 1417 4090
Fragments arranged by size:
4090 1417 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2015 2191 3712 1353
Size: 662 176 1521 3423
Fragments arranged by size:
3423 1521 662 176
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4369 4743 4889 387
Size: 1064 138 2780 374 146 1280
Fragments arranged by size:
2780 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3047 3047
Size: 5782
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2412 3845 4442 5229 5497 1239
Size: 393 780 1433 597 787 268 1524
Fragments arranged by size:
1524 1433 787 780 597 393 268
EcoRI G'AATT_C
Cuts at: 3011 3091 3469 3011
Size: 80 378 5324
Fragments arranged by size:
5324 378 80
FspI TGC'GCA
Cuts at: 4288 4288
Size: 5782
HaeII r_GCGC'y
Cuts at: 1678 2350 4929 1678
Size: 672 2579 2531
Fragments arranged by size:
2579 2531 672
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2473 2505 4423 4455 4497
Size: 32 135 32 901 32 1918 32 42
Cuts at: 4497 4529 1373
Size: 32 2626
Fragments arranged by size:
2626 1918 901 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3126 3372 669
Size: 988 281 1188 246 3079
Fragments arranged by size:
3079 1188 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5782
HpaI GTT'AAC
Cuts at: 3372 3372
Size: 5782
KasI G'GCGC_C
Cuts at: 2346 2346
Size: 5782
KpnI G_GTAC'C
Cuts at: 3107 3107
Size: 5782
MfeI C'AATT_G
Cuts at: 3381 3381
Size: 5782
MluI A'CGCG_T
Cuts at: 5658 5658
Size: 5782
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2195 2419 4896 5080 1305
Size: 890 224 2477 184 2007
Fragments arranged by size:
2477 2007 890 224 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3960
Fragments arranged by size:
3960 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2895 2952 3740 4099 4258 518
Size: 648 1729 57 788 359 159 2042
Fragments arranged by size:
2042 1729 788 648 359 159 57
MspA1I CmG'CkG
Cuts at: 1606 3198 3875 5023 5268 1606
Size: 1592 677 1148 245 2120
Fragments arranged by size:
2120 1592 1148 677 245
NarI GG'CG_CC
Cuts at: 2347 2347
Size: 5782
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5230
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5782
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5782
NotI GC'GGCC_GC
Cuts at: 3229 3229
Size: 5782
NsiI A_TGCA'T
Cuts at: 2731 2731
Size: 5782
NspI r_CATG'y
Cuts at: 1132 1229 3140 4685 1132
Size: 97 1911 1545 2229
Fragments arranged by size:
2229 1911 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5782
PciI A'CATG_T
Cuts at: 4681 4681
Size: 5782
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5782
PmeI GTTT'AAAC
Cuts at: 3086 3086
Size: 5782
PmlI CAC'GTG
Cuts at: 2713 2713
Size: 5782
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3851 3883 5364 5396 3851
Size: 32 1481 32 4237
Fragments arranged by size:
4237 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5782
PsiI TTA'TAA
Cuts at: 2064 3352 2064
Size: 1288 4494
PstI C_TGCA'G
Cuts at: 830 2542 3134 830
Size: 1712 592 3478
Fragments arranged by size:
3478 1712 592
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5782
SacI G_AGCT'C
Cuts at: 3049 3049
Size: 5782
SalI G'TCGA_C
Cuts at: 1936 3124 1936
Size: 1188 4594
SapI GCTCTTCn'nnn_
Cuts at: 2191 2191
Size: 5782
SbfI CC_TGCA'GG
Cuts at: 3134 3134
Size: 5782
ScaI AGT'ACT
Cuts at: 4030 5742 4030
Size: 1712 4070
SfcI C'TryA_G
Cuts at: 826 1045 2538 3130 4265 4946 5137 826
Size: 219 1493 592 1135 681 191 1471
Fragments arranged by size:
1493 1471 1135 681 592 219 191
SfoI GGC'GCC
Cuts at: 2348 2348
Size: 5782
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5782
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5782
SmaI CCC'GGG
Cuts at: 1872 3109 1872
Size: 1237 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2738 3886 4787 5049 820
Size: 197 926 795 1148 901 262 1553
Fragments arranged by size:
1553 1148 926 901 795 262 197
SnaBI TAC'GTA
Cuts at: 493 3573 493
Size: 3080 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5782
SphI G_CATG'C
Cuts at: 1132 3140 1132
Size: 2008 3774
SspI AAT'ATT
Cuts at: 5 52 3706 5
Size: 47 3654 2081
Fragments arranged by size:
3654 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5782
StyI C'CwwG_G
Cuts at: 513 1065 3221 513
Size: 552 2156 3074
Fragments arranged by size:
3074 2156 552
SwaI ATTT'AAAT
Cuts at: 2925 2925
Size: 5782
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2130 3809 3826 3979 4164 4609 325
Size: 1255 550 1679 17 153 185 445 1498
Fragments arranged by size:
1679 1498 1255 550 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2587 4028 5740
Size: 274 80 33 51 1443 610 1441 1712
Cuts at: 5740 96
Size: 138
Fragments arranged by size:
1712 1443 1441 610 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5742 5774 5742
Size: 32 5750
Tth111I GACn'n_nGTC
Cuts at: 1316 2666 1316
Size: 1350 4432
XbaI T'CTAG_A
Cuts at: 3118 3118
Size: 5782
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5782
XmaI C'CCGG_G
Cuts at: 1870 3107 1870
Size: 1237 4545
XmnI GAAnn'nnTTC
Cuts at: 3911 3911
Size: 5782
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3590 276
Size: 53 83 186 936 2056 2468
Fragments arranged by size:
2468 2056 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NarI NcoI
NdeI NheI NotI NsiI NspI PasI PciI PflMI
PmeI PmlI PpiI PshAI PsiI PstI PvuI SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
SwaI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbvCI BplI
Bpu10I BsiWI BstXI EcoNI EcoO109I EcoRV FalI FseI
FspAI NaeI NgoMIV NruI PacI PfoI PpuMI PspOMI
PspXI PsrI PvuII RsrII SacII SanDI SexAI SfiI
SrfI XcmI