Restriction map for
pFN21AB4450
(Circular) (Six-base) MAPSORT of: pFN21AB4450.seq Check: 8110 from: 1 to: 5845
pFN21AB4450 5845 bp
With 182 enzymes: SgfI *
February 27, 2009 14:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2897 2982 2897
Size: 85 5760
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3655 278
Size: 53 83 186 936 2119 2468
Fragments arranged by size:
2468 2119 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3188 1937
Size: 1251 4594
Acc65I G'GTAC_C
Cuts at: 3166 3166
Size: 5845
AclI AA'CG_TT
Cuts at: 1563 3972 4345 1563
Size: 2409 373 3063
Fragments arranged by size:
3063 2409 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3908 5292 5560 1632
Size: 2276 1384 268 1917
Fragments arranged by size:
2276 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5648
AflIII A'CryG_T
Cuts at: 1203 2109 2268 2340 4744 5721 1203
Size: 906 159 72 2404 977 1327
Fragments arranged by size:
2404 1327 977 906 159 72
AgeI A'CCGG_T
Cuts at: 4676 4676
Size: 5845
AhdI GACnn_n'nnGTC
Cuts at: 2050 4574 2050
Size: 2524 3321
AleI CACnn'nnGTG
Cuts at: 1166 2537 2624 1166
Size: 1371 87 4387
Fragments arranged by size:
4387 1371 87
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2849 2987 1184
Size: 1665 138 4042
Fragments arranged by size:
4042 1665 138
AlwNI CAG_nnn'CTG
Cuts at: 3588 5160 3588
Size: 1572 4273
ApaI G_GGCC'C
Cuts at: 2092 3066 2092
Size: 974 4871
ApaLI G'TGCA_C
Cuts at: 3902 5058 3902
Size: 1156 4689
ApoI r'AATT_y
Cuts at: 1493 3154 3380 3532 5641 1493
Size: 1661 226 152 2109 1697
Fragments arranged by size:
2109 1697 1661 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4399 160
Size: 872 3367 1606
Fragments arranged by size:
3367 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5845
AvaI C'yCGr_G
Cuts at: 1870 1943 2083 3170 1870
Size: 73 140 1087 4545
Fragments arranged by size:
4545 1087 140 73
BamHI G'GATC_C
Cuts at: 3175 3542 3175
Size: 367 5478
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2129 2638 2733 3002 3166
Size: 325 217 627 342 509 95 269 164
Cuts at: 3166 4621 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 509 342 325 269 217
164 95
BanII G_rGCy'C
Cuts at: 1391 2092 2582 3066 3164 1391
Size: 701 490 484 98 4072
Fragments arranged by size:
4072 701 490 484 98
BbeI G_GCGC'C
Cuts at: 2737 2737
Size: 5845
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2110 2347 2813 928
Size: 971 211 237 466 3960
Fragments arranged by size:
3960 971 466 237 211
BbvCI CC'TCA_GC
Cuts at: 2372 2423 2372
Size: 51 5794
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4036 4070 1903
Size: 34 2099 34 3678
Fragments arranged by size:
3678 2099 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3737 4947 3737
Size: 1210 4635
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5845
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2367 2483 4456
Size: 107 122 71 631 605 695 116 1973
Cuts at: 4456 136
Size: 1525
Fragments arranged by size:
1973 1525 695 631 605 122 116 107
71
BglII A'GATC_T
Cuts at: 5840 5840
Size: 5845
BlpI GC'TnA_GC
Cuts at: 2191 2315 2744 3262 2191
Size: 124 429 518 4774
Fragments arranged by size:
4774 518 429 124
Bme1580I G_kGCm'C
Cuts at: 1790 2092 2132 2643 3054 3066 3906 5062 1790
Size: 302 40 511 411 12 840 1156 2573
Fragments arranged by size:
2573 1156 840 511 411 302 40 12
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5845
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3576 4534 442
Size: 416 53 83 399 24 2159 958 1753
Fragments arranged by size:
2159 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2214 1056
Size: 1158 4687
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4505 1239
Size: 3266 2579
Bpu10I CC'TnA_GC
Cuts at: 2372 2423 2372
Size: 51 5794
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3970 4835 5133 3970
Size: 865 298 4682
Fragments arranged by size:
4682 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2617 3122 4508 882
Size: 617 1118 505 1386 2219
Fragments arranged by size:
2219 1386 1118 617 505
BsaAI yAC'GTr
Cuts at: 493 1204 2271 3636 493
Size: 711 1067 1365 2702
Fragments arranged by size:
2702 1365 1067 711
BsaBI GATnn'nnATC
Cuts at: 5839 5839
Size: 5845
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2734 3652 4034 4669
Size: 53 83 186 936 1201 918 382 635
Cuts at: 4669 275
Size: 1451
Fragments arranged by size:
1451 1201 936 918 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4278 4676 4950 5097 5508 4278
Size: 398 274 147 411 4615
Fragments arranged by size:
4615 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5620
Fragments arranged by size:
5620 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5845
BseYI C'CCAG_C
Cuts at: 2821 5048 5544 2821
Size: 2227 496 3122
Fragments arranged by size:
3122 2227 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2561 2819 2869 2947 1163
Size: 573 825 258 50 78 4061
Fragments arranged by size:
4061 825 573 258 78 50
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2383 3295 4056 5084 1540
Size: 275 186 382 912 761 1028 2301
Fragments arranged by size:
2301 1028 912 761 382 275 186
BsiHKAI G_wGCw'C
Cuts at: 2154 2582 3906 3991 5062 2154
Size: 428 1324 85 1071 2937
Fragments arranged by size:
2937 1324 1071 428 85
BsmI GAATG_Cn'
Cuts at: 3356 3449 3356
Size: 93 5752
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2092 2132 2154 2582 2643 3054 3066
Size: 399 302 40 22 428 61 411 12
Cuts at: 3066 3164 3906 3991 5062 1391
Size: 98 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 428 411 399 302 98
85 61 40 22 12
BspEI T'CCGG_A
Cuts at: 5508 5508
Size: 5845
BspHI T'CATG_A
Cuts at: 2865 3734 5464 2865
Size: 869 1730 3246
Fragments arranged by size:
3246 1730 869
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2897 2982 3200 844
Size: 2053 85 218 3489
Fragments arranged by size:
3489 2053 218 85
BsrBI CCG'CTC
Cuts at: 1394 1823 3732 1394
Size: 429 1909 3507
Fragments arranged by size:
3507 1909 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2205 4340 4514 65
Size: 1183 957 2135 174 1396
Fragments arranged by size:
2135 1396 1183 957 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2366 2736 4489 4676 1700
Size: 110 556 370 1753 187 2869
Fragments arranged by size:
2869 1753 556 370 187 110
BsrGI T'GTAC_A
Cuts at: 96 2814 96
Size: 2718 3127
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5845
BssSI C'ACGA_G
Cuts at: 3101 3905 4917 3101
Size: 804 1012 4029
Fragments arranged by size:
4029 1012 804
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5845
BstBI TT'CG_AA
Cuts at: 4649 4649
Size: 5845
BstEII G'GTnAC_C
Cuts at: 1184 2767 1184
Size: 1583 4262
BstXI CCAn_nnnn'nTGG
Cuts at: 2626 2626
Size: 5845
BstYI r'GATC_y
Cuts at: 1972 3175 3542 3927 3944 5396 5482 5494 5840
Size: 1203 367 385 17 1452 86 12 346
Cuts at: 5840 1972
Size: 1977
Fragments arranged by size:
1977 1452 1203 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2133 2445 2451 2562 2625 513
Size: 552 939 129 312 6 111 63 3733
Fragments arranged by size:
3733 939 552 312 129 111 63 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2659 2671 487
Size: 683 56 1433 12 3661
Fragments arranged by size:
3661 1433 683 56 12
BtsI GCAGTG_nn'
Cuts at: 2549 3025 3361 4155 4175 2549
Size: 476 336 794 20 4219
Fragments arranged by size:
4219 794 476 336 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5845
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5810
DraI TTT'AAA
Cuts at: 3149 3149
Size: 5845
DraIII CAC_nnn'GTG
Cuts at: 2803 2803
Size: 5845
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4852 809
Size: 507 3536 1802
Fragments arranged by size:
3536 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2391 2409 2448
Size: 54 1425 50 275 18 561 18 39
Cuts at: 2448 2472 2484 2568 2692 2983 3292 4181 5810
Size: 24 12 84 124 291 309 889 1629
Cuts at: 5810 8
Size: 43
Fragments arranged by size:
1629 1425 889 561 309 291 275 124
84 54 50 43 39 24 18 18
12
EagI C'GGCC_G
Cuts at: 1537 1812 3292 1537
Size: 275 1480 4090
Fragments arranged by size:
4090 1480 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2177 2864 3049 3775 1353
Size: 824 687 185 726 3423
Fragments arranged by size:
3423 824 726 687 185
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2942 4432 4806 4952 387
Size: 1064 138 1353 1490 374 146 1280
Fragments arranged by size:
1490 1353 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2580 2580
Size: 5845
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3908 4505 5292 5560 1239
Size: 393 2276 597 787 268 1524
Fragments arranged by size:
2276 1524 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2088 2089 2298 2719 3062 2088
Size: 1 209 421 343 4871
Fragments arranged by size:
4871 421 343 209 1
EcoRI G'AATT_C
Cuts at: 3154 3532 3154
Size: 378 5467
EcoRV GAT'ATC
Cuts at: 2200 2200
Size: 5845
FspI TGC'GCA
Cuts at: 2998 4351 2998
Size: 1353 4492
HaeII r_GCGC'y
Cuts at: 1678 2191 2737 4992 1678
Size: 513 546 2255 2531
Fragments arranged by size:
2531 2255 546 513
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2036 2063 2068 2095 4486
Size: 32 135 32 464 27 5 27 2391
Cuts at: 4486 4518 4560 4592 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2391 464 135 42 32 32 32
32 27 27 5
HincII GTy'rAC
Cuts at: 669 1657 1938 2338 2905 3142 3189 3435 669
Size: 988 281 400 567 237 47 246 3079
Fragments arranged by size:
3079 988 567 400 281 246 237 47
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5845
HpaI GTT'AAC
Cuts at: 3435 3435
Size: 5845
KasI G'GCGC_C
Cuts at: 2733 2733
Size: 5845
KpnI G_GTAC'C
Cuts at: 3170 3170
Size: 5845
MfeI C'AATT_G
Cuts at: 3444 3444
Size: 5845
MluI A'CGCG_T
Cuts at: 5721 5721
Size: 5845
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4959 5143 1305
Size: 3654 184 2007
Fragments arranged by size:
3654 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2393 2411 2450 2486 2570
Size: 54 1425 343 561 18 39 36 84
Cuts at: 2570 2694 2985 10
Size: 124 291 2870
Fragments arranged by size:
2870 1425 561 343 291 124 84 54
39 36 18
MslI CAynn'nnrTG
Cuts at: 518 1166 2108 2537 2624 2651 2753 2756 2969
Size: 648 942 429 87 27 102 3 213
Cuts at: 2969 3803 4162 4321 518
Size: 834 359 159 2042
Fragments arranged by size:
2042 942 834 648 429 359 213 159
102 87 27 3
MspA1I CmG'CkG
Cuts at: 1606 2195 2439 2910 3261 3938 5086 5331 1606
Size: 589 244 471 351 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 589 471 351 245 244
NaeI GCC'GGC
Cuts at: 2738 2738
Size: 5845
NarI GG'CG_CC
Cuts at: 2734 2734
Size: 5845
NcoI C'CATG_G
Cuts at: 513 1065 2004 2562 513
Size: 552 939 558 3796
Fragments arranged by size:
3796 939 558 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5845
NgoMIV G'CCGG_C
Cuts at: 2736 2736
Size: 5845
NheI G'CTAG_C
Cuts at: 1052 2210 1052
Size: 1158 4687
NotI GC'GGCC_GC
Cuts at: 3292 3292
Size: 5845
NspI r_CATG'y
Cuts at: 1132 1229 2113 2344 2821 3203 4748 1132
Size: 97 884 231 477 382 1545 2229
Fragments arranged by size:
2229 1545 884 477 382 231 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5845
PciI A'CATG_T
Cuts at: 2109 2340 4744 2109
Size: 231 2404 3210
Fragments arranged by size:
3210 2404 231
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5845
PmeI GTTT'AAAC
Cuts at: 3149 3149
Size: 5845
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3914 3946 5427 5459 3914
Size: 32 1481 32 4300
Fragments arranged by size:
4300 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2298 2298
Size: 5845
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5845
PsiI TTA'TAA
Cuts at: 3415 3415
Size: 5845
PspOMI G'GGCC_C
Cuts at: 2088 3062 2088
Size: 974 4871
PstI C_TGCA'G
Cuts at: 830 3197 830
Size: 2367 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5845
PvuII CAG'CTG
Cuts at: 2195 2910 2195
Size: 715 5130
SacI G_AGCT'C
Cuts at: 2582 2582
Size: 5845
SalI G'TCGA_C
Cuts at: 1936 3187 1936
Size: 1251 4594
SbfI CC_TGCA'GG
Cuts at: 3197 3197
Size: 5845
ScaI AGT'ACT
Cuts at: 4093 5805 4093
Size: 1712 4133
SexAI A'CCwGG_T
Cuts at: 2799 2799
Size: 5845
SfcI C'TryA_G
Cuts at: 826 1045 3118 3193 4328 5009 5200 826
Size: 219 2073 75 1135 681 191 1471
Fragments arranged by size:
2073 1471 1135 681 219 191 75
SfoI GGC'GCC
Cuts at: 2735 2735
Size: 5845
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5845
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5845
SmaI CCC'GGG
Cuts at: 1872 3172 1872
Size: 1300 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3949 4850 5112 820
Size: 197 926 2006 901 262 1553
Fragments arranged by size:
2006 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3636 493
Size: 3143 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5845
SphI G_CATG'C
Cuts at: 1132 3203 1132
Size: 2071 3774
SspI AAT'ATT
Cuts at: 5 52 3769 5
Size: 47 3717 2081
Fragments arranged by size:
3717 2081 47
StuI AGG'CCT
Cuts at: 1517 2960 1517
Size: 1443 4402
StyI C'CwwG_G
Cuts at: 513 1065 2004 2562 2605 3284 513
Size: 552 939 558 43 679 3074
Fragments arranged by size:
3074 939 679 558 552 43
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2455 3872 3889 4042 4227 4672 325
Size: 1255 875 1417 17 153 185 445 1498
Fragments arranged by size:
1498 1417 1255 875 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2064 2814 4091
Size: 274 80 33 51 1443 87 750 1277
Cuts at: 4091 5803 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1277 750 274 138 87 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3092 3124 5805 5837 3092
Size: 32 2681 32 3100
Fragments arranged by size:
3100 2681 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5845
XbaI T'CTAG_A
Cuts at: 3181 3181
Size: 5845
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2110 2494 2518 2687 3017 2110
Size: 384 24 169 330 4938
Fragments arranged by size:
4938 384 330 169 24
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5845
XmaI C'CCGG_G
Cuts at: 1870 3170 1870
Size: 1300 4545
XmnI GAAnn'nnTTC
Cuts at: 2873 3974 2873
Size: 1101 4744
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3653 276
Size: 53 83 186 936 2119 2468
Fragments arranged by size:
2468 2119 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI
SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI
BsmBI BstZ17I Bsu36I EcoNI FalI FseI FspAI NruI
NsiI PacI PfoI PmlI PspXI PsrI RsrII SacII
SanDI SapI SfiI SrfI SwaI