Restriction map for
pFN21AB4430
(Circular) (Six-base) MAPSORT of: pFN21AB4430.seq Check: 3950 from: 1 to: 6406
pFN21AB4430 6406 bp
With 182 enzymes: SgfI *
February 27, 2009 14:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3247 3247
Size: 6406
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4216 278
Size: 53 83 186 936 2680 2468
Fragments arranged by size:
2680 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3345 3749 1937
Size: 1408 404 4594
Fragments arranged by size:
4594 1408 404
Acc65I G'GTAC_C
Cuts at: 3727 3727
Size: 6406
AclI AA'CG_TT
Cuts at: 1563 4533 4906 1563
Size: 2970 373 3063
Fragments arranged by size:
3063 2970 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2790 2813 2921 3601 3699 4469 5853 6121
Size: 1158 23 108 680 98 770 1384 268
Cuts at: 6121 1632
Size: 1917
Fragments arranged by size:
1917 1384 1158 770 680 268 108 98
23
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6209
AflIII A'CryG_T
Cuts at: 1203 2057 2693 3474 5305 6282 1203
Size: 854 636 781 1831 977 1327
Fragments arranged by size:
1831 1327 977 854 781 636
AgeI A'CCGG_T
Cuts at: 5237 5237
Size: 6406
AhdI GACnn_n'nnGTC
Cuts at: 5135 5135
Size: 6406
AleI CACnn'nnGTG
Cuts at: 1166 2152 2750 3450 1166
Size: 986 598 700 4122
Fragments arranged by size:
4122 986 700 598
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6406
AlwNI CAG_nnn'CTG
Cuts at: 2593 2926 3513 4149 5721 2593
Size: 333 587 636 1572 3278
Fragments arranged by size:
3278 1572 636 587 333
ApaLI G'TGCA_C
Cuts at: 3131 4463 5619 3131
Size: 1332 1156 3918
Fragments arranged by size:
3918 1332 1156
ApoI r'AATT_y
Cuts at: 1493 2159 3307 3501 3715 3941 4093 6202 1493
Size: 666 1148 194 214 226 152 2109 1697
Fragments arranged by size:
2109 1697 1148 666 226 214 194 152
AseI AT'TA_AT
Cuts at: 160 1032 4960 160
Size: 872 3928 1606
Fragments arranged by size:
3928 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6406
AvaI C'yCGr_G
Cuts at: 1870 1943 3069 3646 3731 1870
Size: 73 1126 577 85 4545
Fragments arranged by size:
4545 1126 577 85 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2993 3026 2993
Size: 33 6373
BamHI G'GATC_C
Cuts at: 3736 4103 3736
Size: 367 6039
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3727 5182 618
Size: 325 217 627 1940 1455 1842
Fragments arranged by size:
1940 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3152 3725 1391
Size: 1761 573 4072
Fragments arranged by size:
4072 1761 573
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2873 2951 3687 928
Size: 971 974 78 736 3647
Fragments arranged by size:
3647 974 971 736 78
BbvCI CC'TCA_GC
Cuts at: 2772 2934 2772
Size: 162 6244
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2633 2667 3046 3080 4597 4631 1903
Size: 34 696 34 379 34 1517 34 3678
Fragments arranged by size:
3678 1517 696 379 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4298 5508 4298
Size: 1210 5196
BclI T'GATC_A
Cuts at: 1548 2973 3094 1548
Size: 1425 121 4860
Fragments arranged by size:
4860 1425 121
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5017 136
Size: 107 122 71 631 605 3345 1525
Fragments arranged by size:
3345 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2835 6401 2835
Size: 3566 2840
BlpI GC'TnA_GC
Cuts at: 3823 3823
Size: 6406
Bme1580I G_kGCm'C
Cuts at: 1790 3135 4467 5623 1790
Size: 1345 1332 1156 2573
Fragments arranged by size:
2573 1345 1332 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6406
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2540 3592 4137
Size: 416 53 83 399 24 1123 1052 545
Cuts at: 4137 5095 442
Size: 958 1753
Fragments arranged by size:
1753 1123 1052 958 545 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6406
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2875 2875
Size: 6406
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2903 3267 3647 5066 1239
Size: 1664 364 380 1419 2579
Fragments arranged by size:
2579 1664 1419 380 364
Bpu10I CC'TnA_GC
Cuts at: 2021 2772 2934 2021
Size: 751 162 5493
Fragments arranged by size:
5493 751 162
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3128 4531 5396 5694 3128
Size: 1403 865 298 3840
Fragments arranged by size:
3840 1403 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5069 882
Size: 617 3570 2219
Fragments arranged by size:
3570 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2694 4197 493
Size: 711 1490 1503 2702
Fragments arranged by size:
2702 1503 1490 711
BsaBI GATnn'nnATC
Cuts at: 3125 6400 3125
Size: 3275 3131
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4213 4595 5230 275
Size: 53 83 186 936 2680 382 635 1451
Fragments arranged by size:
2680 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4839 5237 5511 5658 6069 4839
Size: 398 274 147 411 5176
Fragments arranged by size:
5176 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2672 2702 1178
Size: 30 165 30 1269 30 4882
Fragments arranged by size:
4882 1269 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2864 2951 3667 3680 1186
Size: 1678 87 716 13 3912
Fragments arranged by size:
3912 1678 716 87 13
BseYI C'CCAG_C
Cuts at: 3033 3052 3506 3564 5609 6105 3033
Size: 19 454 58 2045 496 3334
Fragments arranged by size:
3334 2045 496 454 58 19
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2024 2154 1163
Size: 573 288 130 5415
Fragments arranged by size:
5415 573 288 130
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3856 4617 5645 1540
Size: 275 186 1855 761 1028 2301
Fragments arranged by size:
2301 1855 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2649 3135 3152 3366 3447 4467 4552 5623 2649
Size: 486 17 214 81 1020 85 1071 3432
Fragments arranged by size:
3432 1071 1020 486 214 85 81 17
BsmI GAATG_Cn'
Cuts at: 3917 4010 3917
Size: 93 6313
BsmBI CGTCTCn'nnnn_
Cuts at: 3542 3593 3542
Size: 51 6355
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2649 3135 3152 3366 3447 3725 4467
Size: 399 859 486 17 214 81 278 742
Cuts at: 4467 4552 5623 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 859 742 486 399 278 214
85 81 17
BspEI T'CCGG_A
Cuts at: 6069 6069
Size: 6406
BspHI T'CATG_A
Cuts at: 4295 6025 4295
Size: 1730 4676
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3051 3188 3247 3761 844
Size: 2207 137 59 514 3489
Fragments arranged by size:
3489 2207 514 137 59
BsrBI CCG'CTC
Cuts at: 1394 1823 2676 2941 4293 1394
Size: 429 853 265 1352 3507
Fragments arranged by size:
3507 1352 853 429 265
BsrDI GCAATG_nn'
Cuts at: 65 1248 2440 4901 5075 65
Size: 1183 1192 2461 174 1396
Fragments arranged by size:
2461 1396 1192 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2580 2612 5050 5237 1700
Size: 110 770 32 2438 187 2869
Fragments arranged by size:
2869 2438 770 187 110 32
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6406
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6406
BssSI C'ACGA_G
Cuts at: 3161 4466 5478 3161
Size: 1305 1012 4089
Fragments arranged by size:
4089 1305 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6406
BstBI TT'CG_AA
Cuts at: 5210 5210
Size: 6406
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6406
BstXI CCAn_nnnn'nTGG
Cuts at: 2662 2740 3358 2662
Size: 78 618 5710
Fragments arranged by size:
5710 618 78
BstYI r'GATC_y
Cuts at: 1972 2835 3736 4103 4488 4505 5957 6043 6055
Size: 863 901 367 385 17 1452 86 12
Cuts at: 6055 6401 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 901 863 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 3351 513
Size: 552 939 1347 3568
Fragments arranged by size:
3568 1347 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5667
Fragments arranged by size:
5667 683 56
BtsI GCAGTG_nn'
Cuts at: 3399 3922 4716 4736 3399
Size: 523 794 20 5069
Fragments arranged by size:
5069 794 523 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6406
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6371
DraI TTT'AAA
Cuts at: 3710 3710
Size: 6406
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2114 5413 809
Size: 507 798 3299 1802
Fragments arranged by size:
3299 1802 798 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2033 3853 4742
Size: 54 1425 50 275 18 203 1820 889
Cuts at: 4742 6371 8
Size: 1629 43
Fragments arranged by size:
1820 1629 1425 889 275 203 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3853 1537
Size: 275 2041 4090
Fragments arranged by size:
4090 2041 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4336 1353
Size: 2983 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4993 5367 5513 387
Size: 1064 138 3404 374 146 1280
Fragments arranged by size:
3404 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3150 3150
Size: 6406
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2790 2813 2903 2921 3267 3601 3647
Size: 393 1158 23 90 18 346 334 46
Cuts at: 3647 3699 4469 5066 5853 6121 1239
Size: 52 770 597 787 268 1524
Fragments arranged by size:
1524 1158 787 770 597 393 346 334
268 90 52 46 23 18
EcoO109I rG'GnC_Cy
Cuts at: 2026 3040 3139 2026
Size: 1014 99 5293
Fragments arranged by size:
5293 1014 99
EcoRI G'AATT_C
Cuts at: 3715 4093 3715
Size: 378 6028
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2899 2899
Size: 6406
FspI TGC'GCA
Cuts at: 3047 4912 3047
Size: 1865 4541
HaeII r_GCGC'y
Cuts at: 1678 5553 1678
Size: 3875 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2660 2672 2692 2704 2870
Size: 32 135 32 1088 12 20 12 166
Cuts at: 2870 2902 3332 3364 3629 3661 5047 5079 5121
Size: 32 430 32 265 32 1386 32 42
Cuts at: 5121 5153 1373
Size: 32 2626
Fragments arranged by size:
2626 1386 1088 430 265 166 135 42
32 32 32 32 32 32 32 20
12 12
HincII GTy'rAC
Cuts at: 669 1657 1938 2578 3555 3750 3996 669
Size: 988 281 640 977 195 246 3079
Fragments arranged by size:
3079 988 977 640 281 246 195
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6406
HpaI GTT'AAC
Cuts at: 2578 3996 2578
Size: 1418 4988
KpnI G_GTAC'C
Cuts at: 3731 3731
Size: 6406
MfeI C'AATT_G
Cuts at: 4005 4005
Size: 6406
MluI A'CGCG_T
Cuts at: 2057 6282 2057
Size: 4225 2181
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5520 5704 1305
Size: 4215 184 2007
Fragments arranged by size:
4215 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4584
Fragments arranged by size:
4584 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2152 2750 3450 3536 4364 4723 4882
Size: 648 986 598 700 86 828 359 159
Cuts at: 4882 518
Size: 2042
Fragments arranged by size:
2042 986 828 700 648 598 359 159
86
MspA1I CmG'CkG
Cuts at: 1606 2038 3033 3822 4499 5647 5892 1606
Size: 432 995 789 677 1148 245 2120
Fragments arranged by size:
2120 1148 995 789 677 432 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 3351 513
Size: 552 939 1347 3568
Fragments arranged by size:
3568 1347 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6406
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6406
NotI GC'GGCC_GC
Cuts at: 3853 3853
Size: 6406
NspI r_CATG'y
Cuts at: 1132 1229 3026 3764 5309 1132
Size: 97 1797 738 1545 2229
Fragments arranged by size:
2229 1797 1545 738 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6406
PciI A'CATG_T
Cuts at: 5305 5305
Size: 6406
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3513 3604 1108
Size: 2405 91 3910
Fragments arranged by size:
3910 2405 91
PfoI T'CCnGG_A
Cuts at: 2661 3299 2661
Size: 638 5768
PmeI GTTT'AAAC
Cuts at: 3710 3710
Size: 6406
PmlI CAC'GTG
Cuts at: 2694 2694
Size: 6406
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4475 4507 5988 6020 4475
Size: 32 1481 32 4861
Fragments arranged by size:
4861 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3040 3139 3040
Size: 99 6307
PshAI GACnn'nnGTC
Cuts at: 1613 3341 1613
Size: 1728 4678
PsiI TTA'TAA
Cuts at: 3976 3976
Size: 6406
PstI C_TGCA'G
Cuts at: 830 2219 3758 830
Size: 1389 1539 3478
Fragments arranged by size:
3478 1539 1389
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6406
PvuII CAG'CTG
Cuts at: 3033 3033
Size: 6406
SacI G_AGCT'C
Cuts at: 3152 3152
Size: 6406
SalI G'TCGA_C
Cuts at: 1936 3748 1936
Size: 1812 4594
SbfI CC_TGCA'GG
Cuts at: 3758 3758
Size: 6406
ScaI AGT'ACT
Cuts at: 4654 6366 4654
Size: 1712 4694
SfcI C'TryA_G
Cuts at: 826 1045 2215 2416 3002 3754 4889 5570 5761
Size: 219 1170 201 586 752 1135 681 191
Cuts at: 5761 826
Size: 1471
Fragments arranged by size:
1471 1170 1135 752 681 586 219 201
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6406
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6406
SmaI CCC'GGG
Cuts at: 1872 3071 3733 1872
Size: 1199 662 4545
Fragments arranged by size:
4545 1199 662
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3143 4510 5411 5673 820
Size: 197 926 1200 1367 901 262 1553
Fragments arranged by size:
1553 1367 1200 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4197 493
Size: 3704 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6406
SphI G_CATG'C
Cuts at: 1132 3764 1132
Size: 2632 3774
SspI AAT'ATT
Cuts at: 5 52 4330 5
Size: 47 4278 2081
Fragments arranged by size:
4278 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6406
StyI C'CwwG_G
Cuts at: 513 1065 2004 2991 3351 3432 3845 513
Size: 552 939 987 360 81 413 3074
Fragments arranged by size:
3074 987 939 552 413 360 81
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3353 4433 4450 4603 4788 5233 325
Size: 1255 1773 1080 17 153 185 445 1498
Fragments arranged by size:
1773 1498 1255 1080 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4652 6364 96
Size: 274 80 33 51 1443 2675 1712 138
Fragments arranged by size:
2675 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2458 2490 6366 6398 2458
Size: 32 3876 32 2466
Fragments arranged by size:
3876 2466 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6406
XbaI T'CTAG_A
Cuts at: 3742 3742
Size: 6406
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3307 3307
Size: 6406
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6406
XmaI C'CCGG_G
Cuts at: 1870 3069 3731 1870
Size: 1199 662 4545
Fragments arranged by size:
4545 1199 662
XmnI GAAnn'nnTTC
Cuts at: 4535 4535
Size: 6406
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4214 276
Size: 53 83 186 936 2680 2468
Fragments arranged by size:
2680 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BbeI BfrBI BsiWI
BstZ17I Bsu36I DraIII EcoNI EcoRV FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PspOMI PspXI
PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI