Restriction map for
pFN21AB4426
(Circular) (Six-base) MAPSORT of: pFN21AB4426.seq Check: 2764 from: 1 to: 5521
pFN21AB4426 5521 bp
With 182 enzymes: SgfI *
January 16, 2009 15:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3331 278
Size: 53 83 186 936 1795 2468
Fragments arranged by size:
2468 1795 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2211 2864 1937
Size: 274 653 4594
Fragments arranged by size:
4594 653 274
Acc65I G'GTAC_C
Cuts at: 2842 2842
Size: 5521
AclI AA'CG_TT
Cuts at: 1563 2814 3648 4021 1563
Size: 1251 834 373 3063
Fragments arranged by size:
3063 1251 834 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2465 2472 3584 4968 5236 1632
Size: 833 7 1112 1384 268 1917
Fragments arranged by size:
1917 1384 1112 833 268 7
AfeI AGC'GCT
Cuts at: 2222 2222
Size: 5521
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5324
AflIII A'CryG_T
Cuts at: 1203 4420 5397 1203
Size: 3217 977 1327
Fragments arranged by size:
3217 1327 977
AgeI A'CCGG_T
Cuts at: 4352 4352
Size: 5521
AhdI GACnn_n'nnGTC
Cuts at: 4250 4250
Size: 5521
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5521
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5521
AlwNI CAG_nnn'CTG
Cuts at: 2065 2236 3264 4836 2065
Size: 171 1028 1572 2750
Fragments arranged by size:
2750 1572 1028 171
ApaI G_GGCC'C
Cuts at: 2576 2576
Size: 5521
ApaLI G'TGCA_C
Cuts at: 2078 3578 4734 2078
Size: 1500 1156 2865
Fragments arranged by size:
2865 1500 1156
ApoI r'AATT_y
Cuts at: 1493 2830 3056 3208 5317 1493
Size: 1337 226 152 2109 1697
Fragments arranged by size:
2109 1697 1337 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4075 160
Size: 872 3043 1606
Fragments arranged by size:
3043 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5521
AvaI C'yCGr_G
Cuts at: 1870 1943 2846 1870
Size: 73 903 4545
Fragments arranged by size:
4545 903 73
BamHI G'GATC_C
Cuts at: 2851 3218 2851
Size: 367 5154
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2632 2842 4297 618
Size: 325 217 627 845 210 1455 1842
Fragments arranged by size:
1842 1455 845 627 325 217 210
BanII G_rGCy'C
Cuts at: 1391 2072 2091 2370 2576 2613 2840 1391
Size: 681 19 279 206 37 227 4072
Fragments arranged by size:
4072 681 279 227 206 37 19
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4550
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2501 2535 3712 3746 1903
Size: 34 564 34 1177 34 3678
Fragments arranged by size:
3678 1177 564 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3413 4623 3413
Size: 1210 4311
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5521
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2133 4132 136
Size: 107 122 71 631 605 461 1999 1525
Fragments arranged by size:
1999 1525 631 605 461 122 107 71
BglII A'GATC_T
Cuts at: 2409 5516 2409
Size: 3107 2414
BlpI GC'TnA_GC
Cuts at: 2938 2938
Size: 5521
Bme1580I G_kGCm'C
Cuts at: 1790 2082 2120 2576 2637 3582 4738 1790
Size: 292 38 456 61 945 1156 2573
Fragments arranged by size:
2573 1156 945 456 292 61 38
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5521
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2482 3252 4210
Size: 416 53 83 399 24 1065 770 958
Cuts at: 4210 442
Size: 1753
Fragments arranged by size:
1753 1065 958 770 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5521
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2365 2416 2365
Size: 51 5470
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4181 1239
Size: 2942 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3646 4511 4809 3646
Size: 865 298 4358
Fragments arranged by size:
4358 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2387 4184 882
Size: 617 888 1797 2219
Fragments arranged by size:
2219 1797 888 617
BsaAI yAC'GTr
Cuts at: 493 1204 3312 493
Size: 711 2108 2702
Fragments arranged by size:
2702 2108 711
BsaBI GATnn'nnATC
Cuts at: 5515 5515
Size: 5521
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2208 3328 3710 4345
Size: 53 83 186 936 675 1120 382 635
Cuts at: 4345 275
Size: 1451
Fragments arranged by size:
1451 1120 936 675 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3954 4352 4626 4773 5184 3954
Size: 398 274 147 411 4291
Fragments arranged by size:
4291 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5296
Fragments arranged by size:
5296 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2276 2378 2405 1186
Size: 1090 102 27 4302
Fragments arranged by size:
4302 1090 102 27
BseYI C'CCAG_C
Cuts at: 2123 4724 5220 2123
Size: 2601 496 2424
Fragments arranged by size:
2601 2424 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2258 2270 2526 1163
Size: 573 522 12 256 4158
Fragments arranged by size:
4158 573 522 256 12
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2293 2971 3732 4760 1540
Size: 275 186 292 678 761 1028 2301
Fragments arranged by size:
2301 1028 761 678 292 275 186
BsiHKAI G_wGCw'C
Cuts at: 2082 2361 2613 3582 3667 4738 2082
Size: 279 252 969 85 1071 2865
Fragments arranged by size:
2865 1071 969 279 252 85
BsmI GAATG_Cn'
Cuts at: 3032 3125 3032
Size: 93 5428
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2072 2082 2091 2120 2361 2370 2576
Size: 399 282 10 9 29 241 9 206
Cuts at: 2576 2613 2637 2840 3582 3667 4738 1391
Size: 37 24 203 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 282 241 206 203
85 37 29 24 10 9 9
BspEI T'CCGG_A
Cuts at: 5184 5184
Size: 5521
BspHI T'CATG_A
Cuts at: 3410 5140 3410
Size: 1730 3791
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2186 2658 2876 844
Size: 1342 472 218 3489
Fragments arranged by size:
3489 1342 472 218
BsrBI CCG'CTC
Cuts at: 1394 1823 3408 1394
Size: 429 1585 3507
Fragments arranged by size:
3507 1585 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4016 4190 65
Size: 1183 2768 174 1396
Fragments arranged by size:
2768 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2053 4165 4352 1700
Size: 110 243 2112 187 2869
Fragments arranged by size:
2869 2112 243 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5521
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5521
BssSI C'ACGA_G
Cuts at: 2360 3581 4593 2360
Size: 1221 1012 3288
Fragments arranged by size:
3288 1221 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2178 2281 794
Size: 1384 103 4034
Fragments arranged by size:
4034 1384 103
BstBI TT'CG_AA
Cuts at: 4325 4325
Size: 5521
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5521
BstXI CCAn_nnnn'nTGG
Cuts at: 2131 2131
Size: 5521
BstYI r'GATC_y
Cuts at: 1972 2409 2851 3218 3603 3620 5072 5158 5170
Size: 437 442 367 385 17 1452 86 12
Cuts at: 5170 5516 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 442 437 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2019 2293 2704 513
Size: 552 939 15 274 411 3330
Fragments arranged by size:
3330 939 552 411 274 15
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4782
Fragments arranged by size:
4782 683 56
BtsI GCAGTG_nn'
Cuts at: 2080 2463 2517 2696 3037 3831 3851 2080
Size: 383 54 179 341 794 20 3750
Fragments arranged by size:
3750 794 383 341 179 54 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5521
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5486
DraI TTT'AAA
Cuts at: 2825 2825
Size: 5521
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4528 809
Size: 507 3212 1802
Fragments arranged by size:
3212 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2037 2187 2290
Size: 54 1425 50 275 18 207 150 103
Cuts at: 2290 2385 2707 2968 3857 5486 8
Size: 95 322 261 889 1629 43
Fragments arranged by size:
1629 1425 889 322 275 261 207 150
103 95 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2290 2968 1537
Size: 275 478 678 4090
Fragments arranged by size:
4090 678 478 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3451 1353
Size: 2098 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4108 4482 4628 387
Size: 1064 138 2519 374 146 1280
Fragments arranged by size:
2519 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2611 2611
Size: 5521
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2465 2472 3584 4181 4968 5236 1239
Size: 393 833 7 1112 597 787 268 1524
Fragments arranged by size:
1524 1112 833 787 597 393 268 7
EcoNI CCTnn'n_nnAGG
Cuts at: 2189 2189
Size: 5521
EcoO109I rG'GnC_Cy
Cuts at: 2572 2573 2572
Size: 1 5520
EcoRI G'AATT_C
Cuts at: 2830 3208 2830
Size: 378 5143
FseI GG_CCGG'CC
Cuts at: 2057 2057
Size: 5521
FspI TGC'GCA
Cuts at: 4027 4027
Size: 5521
HaeII r_GCGC'y
Cuts at: 1678 2224 4668 1678
Size: 546 2444 2531
Fragments arranged by size:
2531 2444 546
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2183 2215 4162 4194 4236
Size: 32 135 32 611 32 1947 32 42
Cuts at: 4236 4268 1373
Size: 32 2626
Fragments arranged by size:
2626 1947 611 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2776 2865 3111 669
Size: 988 281 838 89 246 3079
Fragments arranged by size:
3079 988 838 281 246 89
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5521
HpaI GTT'AAC
Cuts at: 3111 3111
Size: 5521
KpnI G_GTAC'C
Cuts at: 2846 2846
Size: 5521
MfeI C'AATT_G
Cuts at: 2656 3120 2656
Size: 464 5057
MluI A'CGCG_T
Cuts at: 5397 5397
Size: 5521
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4635 4819 1305
Size: 3330 184 2007
Fragments arranged by size:
3330 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3699
Fragments arranged by size:
3699 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3479 3838 3997 518
Size: 648 2313 359 159 2042
Fragments arranged by size:
2313 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2021 2272 2295 2937 3614 4762 5007 1606
Size: 415 251 23 642 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 642 415 251 245 23
NaeI GCC'GGC
Cuts at: 2055 2055
Size: 5521
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4030
Fragments arranged by size:
4030 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5521
NgoMIV G'CCGG_C
Cuts at: 2053 2053
Size: 5521
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5521
NotI GC'GGCC_GC
Cuts at: 2968 2968
Size: 5521
NspI r_CATG'y
Cuts at: 1132 1229 2879 4424 1132
Size: 97 1650 1545 2229
Fragments arranged by size:
2229 1650 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2131 2230 1348
Size: 783 99 4639
Fragments arranged by size:
4639 783 99
PciI A'CATG_T
Cuts at: 4420 4420
Size: 5521
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2065 1108
Size: 957 4564
PmeI GTTT'AAAC
Cuts at: 2825 2825
Size: 5521
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3590 3622 5103 5135 3590
Size: 32 1481 32 3976
Fragments arranged by size:
3976 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2560 1613
Size: 947 4574
PsiI TTA'TAA
Cuts at: 3091 3091
Size: 5521
PspOMI G'GGCC_C
Cuts at: 2572 2572
Size: 5521
PstI C_TGCA'G
Cuts at: 830 2607 2873 830
Size: 1777 266 3478
Fragments arranged by size:
3478 1777 266
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5521
SacI G_AGCT'C
Cuts at: 2613 2613
Size: 5521
SacII CC_GC'GG
Cuts at: 2022 2296 2022
Size: 274 5247
SalI G'TCGA_C
Cuts at: 1936 2863 1936
Size: 927 4594
SbfI CC_TGCA'GG
Cuts at: 2607 2873 2607
Size: 266 5255
ScaI AGT'ACT
Cuts at: 3769 5481 3769
Size: 1712 3809
SfcI C'TryA_G
Cuts at: 826 1045 2603 2869 4004 4685 4876 826
Size: 219 1558 266 1135 681 191 1471
Fragments arranged by size:
1558 1471 1135 681 266 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5521
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5521
SmaI CCC'GGG
Cuts at: 1872 2848 1872
Size: 976 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3625 4526 4788 820
Size: 197 926 1682 901 262 1553
Fragments arranged by size:
1682 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3312 493
Size: 2819 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5521
SphI G_CATG'C
Cuts at: 1132 2879 1132
Size: 1747 3774
SspI AAT'ATT
Cuts at: 5 52 3445 5
Size: 47 3393 2081
Fragments arranged by size:
3393 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5521
StyI C'CwwG_G
Cuts at: 513 1065 2004 2960 513
Size: 552 939 956 3074
Fragments arranged by size:
3074 956 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3548 3565 3718 3903 4348 325
Size: 1255 1968 17 153 185 445 1498
Fragments arranged by size:
1968 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3767 5479 96
Size: 274 80 33 51 1443 1790 1712 138
Fragments arranged by size:
1790 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2774 2806 5481 5513 2774
Size: 32 2675 32 2782
Fragments arranged by size:
2782 2675 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2488 1316
Size: 1172 4349
XbaI T'CTAG_A
Cuts at: 2857 2857
Size: 5521
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2380 2626 2380
Size: 246 5275
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5521
XmaI C'CCGG_G
Cuts at: 1870 2846 1870
Size: 976 4545
XmnI GAAnn'nnTTC
Cuts at: 3650 3650
Size: 5521
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3329 276
Size: 53 83 186 936 1795 2468
Fragments arranged by size:
2468 1795 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FseI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PmeI
PpiI PshAI PsiI PspOMI PstI PvuI SacI SacII
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV FalI
FspAI KasI NarI NruI NsiI PacI PfoI PmlI
PpuMI PspXI PsrI PvuII RsrII SanDI SapI SexAI
SfiI SfoI SrfI SwaI