Restriction map for
pFN21AB4399
(Circular) (Six-base) MAPSORT of: pFN21AB4399.seq Check: 5793 from: 1 to: 11689
pFN21AB4399 11689 bp
With 182 enzymes: SgfI *
February 27, 2009 14:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 6696 7551 7951 6696
Size: 855 400 10434
Fragments arranged by size:
10434 855 400
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 9499 278
Size: 53 83 186 936 7963 2468
Fragments arranged by size:
7963 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 9032 1937
Size: 7095 4594
Acc65I G'GTAC_C
Cuts at: 3801 9010 3801
Size: 5209 6480
AclI AA'CG_TT
Cuts at: 1563 9816 10189 1563
Size: 8253 373 3063
Fragments arranged by size:
8253 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2699 3019 3800 4208 4289 5565 6287 6893
Size: 1067 320 781 408 81 1276 722 606
Cuts at: 6893 6980 7049 7714 7864 9752 11136 11404 1632
Size: 87 69 665 150 1888 1384 268 1917
Fragments arranged by size:
1917 1888 1384 1276 1067 781 722 665
606 408 320 268 150 87 81 69
AfeI AGC'GCT
Cuts at: 8195 8195
Size: 11689
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 11492
AflIII A'CryG_T
Cuts at: 1203 7068 10588 11565 1203
Size: 5865 3520 977 1327
Fragments arranged by size:
5865 3520 1327 977
AgeI A'CCGG_T
Cuts at: 10520 10520
Size: 11689
AhdI GACnn_n'nnGTC
Cuts at: 10418 10418
Size: 11689
AleI CACnn'nnGTG
Cuts at: 1166 2810 1166
Size: 1644 10045
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 5328 6183 6548 6912 7394 1184
Size: 4144 855 365 364 482 5479
Fragments arranged by size:
5479 4144 855 482 365 364
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2390 2422 2390
Size: 32 11657
AlwNI CAG_nnn'CTG
Cuts at: 3025 3189 3466 3550 4480 4534 4828 5065 5140
Size: 164 277 84 930 54 294 237 75
Cuts at: 5140 5212 5291 5302 5431 5618 6571 6634 6742
Size: 72 79 11 129 187 953 63 108
Cuts at: 6742 6952 7381 7666 7887 8380 8610 8902 9432
Size: 210 429 285 221 493 230 292 530
Cuts at: 9432 11004 3025
Size: 1572 3710
Fragments arranged by size:
3710 1572 953 930 530 493 429 294
292 285 277 237 230 221 210 187
164 129 108 84 79 75 72 63
54 11
ApaI G_GGCC'C
Cuts at: 4345 6946 8586 4345
Size: 2601 1640 7448
Fragments arranged by size:
7448 2601 1640
ApaLI G'TGCA_C
Cuts at: 4871 7514 9746 10902 4871
Size: 2643 2232 1156 5658
Fragments arranged by size:
5658 2643 2232 1156
ApoI r'AATT_y
Cuts at: 1493 7718 8998 9224 9376 11485 1493
Size: 6225 1280 226 152 2109 1697
Fragments arranged by size:
6225 2109 1697 1280 226 152
AseI AT'TA_AT
Cuts at: 160 1032 10243 160
Size: 872 9211 1606
Fragments arranged by size:
9211 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 11689
AvaI C'yCGr_G
Cuts at: 1870 1943 2211 3575 6223 7212 7466 8047 8347
Size: 73 268 1364 2648 989 254 581 300
Cuts at: 8347 9014 1870
Size: 667 4545
Fragments arranged by size:
4545 2648 1364 989 667 581 300 268
254 73
AvrII C'CTAG_G
Cuts at: 6403 6403
Size: 11689
BamHI G'GATC_C
Cuts at: 5622 9019 9386 5622
Size: 3397 367 7925
Fragments arranged by size:
7925 3397 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3564 3801 4134 5340 6328
Size: 325 217 627 1777 237 333 1206 988
Cuts at: 6328 6684 6857 7421 7686 9010 10465 618
Size: 356 173 564 265 1324 1455 1842
Fragments arranged by size:
1842 1777 1455 1324 1206 988 627 564
356 333 325 265 237 217 173
BanII G_rGCy'C
Cuts at: 1391 2408 3246 3445 4062 4345 4491 5592 5862
Size: 1017 838 199 617 283 146 1101 270
Cuts at: 5862 6146 6698 6819 6946 7359 7539 8094 8457
Size: 284 552 121 127 413 180 555 363
Cuts at: 8457 8502 8586 9008 1391
Size: 45 84 422 4072
Fragments arranged by size:
4072 1101 1017 838 617 555 552 422
413 363 284 283 270 199 180 146
127 121 84 45
BbeI G_GCGC'C
Cuts at: 3568 6861 7690 3568
Size: 3293 829 7567
Fragments arranged by size:
7567 3293 829
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2567 3702 4703 6122 928
Size: 971 668 1135 1001 1419 6495
Fragments arranged by size:
6495 1419 1135 1001 971 668
BbvCI CC'TCA_GC
Cuts at: 5555 5712 6792 8067 8777 5555
Size: 157 1080 1275 710 8467
Fragments arranged by size:
8467 1275 1080 710 157
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2192 2202 2226 2236 2854 2888 3401
Size: 34 255 10 24 10 618 34 513
Cuts at: 3401 3435 7661 7695 9880 9914 1903
Size: 34 4226 34 2185 34 3678
Fragments arranged by size:
4226 3678 2185 618 513 255 34 34
34 34 34 24 10 10
BciVI GTATCCnnnnn_n'
Cuts at: 5437 9581 10791 5437
Size: 4144 1210 6335
Fragments arranged by size:
6335 4144 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 11689
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2739 4340 5027
Size: 107 122 71 631 605 1067 1601 687
Cuts at: 5027 5540 5798 6758 6941 7493 8063 8186 10300
Size: 513 258 960 183 552 570 123 2114
Cuts at: 10300 136
Size: 1525
Fragments arranged by size:
2114 1601 1525 1067 960 687 631 605
570 552 513 258 183 123 122 107
71
BglII A'GATC_T
Cuts at: 3978 4089 11684 3978
Size: 111 7595 3983
Fragments arranged by size:
7595 3983 111
BlpI GC'TnA_GC
Cuts at: 2110 2524 2727 3869 5292 6261 9106 2110
Size: 414 203 1142 1423 969 2845 4693
Fragments arranged by size:
4693 2845 1423 1142 969 414 203
Bme1580I G_kGCm'C
Cuts at: 1790 4137 4345 4875 5343 6687 6946 7518 8586
Size: 2347 208 530 468 1344 259 572 1068
Cuts at: 8586 9750 10906 1790
Size: 1164 1156 2573
Fragments arranged by size:
2573 2347 1344 1164 1156 1068 572 530
468 259 208
BmgBI CAC'GTC
Cuts at: 1318 5014 7036 7474 7576 8767 1318
Size: 3696 2022 438 102 1191 4240
Fragments arranged by size:
4240 3696 2022 1191 438 102
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 6031 6502 8280
Size: 416 53 83 399 24 4614 471 1778
Cuts at: 8280 8439 9420 10378 442
Size: 159 981 958 1753
Fragments arranged by size:
4614 1778 1753 981 958 471 416 399
159 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2936 1056
Size: 1880 9809
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2305 6516 2305
Size: 4211 7478
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2300 2313 2348 2363 2414 2587 2726 3221
Size: 1061 13 35 15 51 173 139 495
Cuts at: 3221 3467 3488 3923 4103 4205 4391 4400 4430
Size: 246 21 435 180 102 186 9 30
Cuts at: 4430 4664 4673 5024 5066 5453 5828 5846 7082
Size: 234 9 351 42 387 375 18 1236
Cuts at: 7082 7130 7373 7913 8051 8228 8295 8597 10349
Size: 48 243 540 138 177 67 302 1752
Cuts at: 10349 1239
Size: 2579
Fragments arranged by size:
2579 1752 1236 1061 540 495 435 387
375 351 302 246 243 234 186 180
177 173 139 138 102 67 51 48
42 35 30 21 18 15 13 9
9
Bpu10I CC'TnA_GC
Cuts at: 3459 5082 5555 5712 6792 8067 8633 8777 3459
Size: 1623 473 157 1080 1275 566 144 6371
Fragments arranged by size:
6371 1623 1275 1080 566 473 157 144
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2357 5698 6385 6689 7073 8246 8262 9814 10679
Size: 3341 687 304 384 1173 16 1552 865
Cuts at: 10679 10977 2357
Size: 298 3069
Fragments arranged by size:
3341 3069 1552 1173 865 687 384 304
298 16
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3302 3401 3572 4892 7091 7211 7778
Size: 617 1803 99 171 1320 2199 120 567
Cuts at: 7778 8248 10352 882
Size: 470 2104 2219
Fragments arranged by size:
2219 2199 2104 1803 1320 617 567 470
171 120 99
BsaAI yAC'GTr
Cuts at: 493 1204 7177 9480 493
Size: 711 5973 2303 2702
Fragments arranged by size:
5973 2702 2303 711
BsaBI GATnn'nnATC
Cuts at: 2330 11683 2330
Size: 9353 2336
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2076 3012 3565 4287
Size: 53 83 186 936 543 936 553 722
Cuts at: 4287 4578 6750 6858 7485 7687 8931 9496 9878
Size: 291 2172 108 627 202 1244 565 382
Cuts at: 9878 10513 275
Size: 635 1451
Fragments arranged by size:
2172 1451 1244 936 936 722 635 627
565 553 543 382 291 202 186 108
83 53
BsaWI w'CCGG_w
Cuts at: 3288 5625 10122 10520 10794 10941 11352 3288
Size: 2337 4497 398 274 147 411 3625
Fragments arranged by size:
4497 3625 2337 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2390 2420 2585 2615 2819
Size: 30 165 30 987 30 165 30 204
Cuts at: 2819 2849 5039 5069 5114 5144 6812 6842 7055
Size: 30 2190 30 45 30 1668 30 213
Cuts at: 7055 7085 7736 7766 8819 8849 1178
Size: 30 651 30 1053 30 4018
Fragments arranged by size:
4018 2190 1668 1053 987 651 213 204
165 165 45 30 30 30 30 30
30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2744 3443 3878 4388 4397 4910 5138 6317
Size: 1558 699 435 510 9 513 228 1179
Cuts at: 6317 6359 6366 6503 6686 7739 7801 7952 8279
Size: 42 7 137 183 1053 62 151 327
Cuts at: 8279 8297 8837 8840 1186
Size: 18 540 3 4035
Fragments arranged by size:
4035 1558 1179 1053 699 540 513 510
435 327 228 183 151 137 62 42
18 9 7 3
BseYI C'CCAG_C
Cuts at: 2070 2737 3884 5058 5173 5618 6120 6307 7546
Size: 667 1147 1174 115 445 502 187 1239
Cuts at: 7546 7604 7695 8007 8184 10892 11388 2070
Size: 58 91 312 177 2708 496 2371
Fragments arranged by size:
2708 2371 1239 1174 1147 667 502 496
445 312 187 177 115 91 58
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3602 4682 5771 6059 7385 7580 8561
Size: 573 1866 1080 1089 288 1326 195 981
Cuts at: 8561 1163
Size: 4291
Fragments arranged by size:
4291 1866 1326 1089 1080 981 573 288
195
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 6726 6916 7497 9139 9900 10928
Size: 275 186 4725 190 581 1642 761 1028
Cuts at: 10928 1540
Size: 2301
Fragments arranged by size:
4725 2301 1642 1028 761 581 275 190
186
BsiHKAI G_wGCw'C
Cuts at: 2289 2712 3246 4023 4062 4407 4491 4875 5862
Size: 423 534 777 39 345 84 384 987
Cuts at: 5862 6146 6698 6819 7359 7518 7539 8634 8858
Size: 284 552 121 540 159 21 1095 224
Cuts at: 8858 9750 9835 10906 2289
Size: 892 85 1071 3072
Fragments arranged by size:
3072 1095 1071 987 892 777 552 540
534 423 384 345 284 224 159 121
85 84 39 21
BsmI GAATG_Cn'
Cuts at: 2714 5461 7261 9200 9293 2714
Size: 2747 1800 1939 93 5110
Fragments arranged by size:
5110 2747 1939 1800 93
BsmBI CGTCTCn'nnnn_
Cuts at: 6467 6467
Size: 11689
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2289 2408 2712 3246 3445 4023 4062
Size: 399 499 119 304 534 199 578 39
Cuts at: 4062 4137 4345 4407 4491 4875 5343 5592 5862
Size: 75 208 62 84 384 468 249 270
Cuts at: 5862 6146 6687 6698 6819 6946 7359 7518 7539
Size: 284 541 11 121 127 413 159 21
Cuts at: 7539 8094 8457 8502 8586 8634 8858 9008 9750
Size: 555 363 45 84 48 224 150 742
Cuts at: 9750 9835 10906 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 578 555 541 534 499
468 413 399 384 363 304 284 270
249 224 208 199 159 150 127 121
119 85 84 84 75 62 48 45
39 21 11
BspEI T'CCGG_A
Cuts at: 5625 11352 5625
Size: 5727 5962
BspHI T'CATG_A
Cuts at: 6552 7827 9578 11308 6552
Size: 1275 1751 1730 6933
Fragments arranged by size:
6933 1751 1730 1275
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2256 2952 3549 3708 4527 4958 5306 6159
Size: 1412 696 597 159 819 431 348 853
Cuts at: 6159 6696 7407 7551 7951 8388 9044 844
Size: 537 711 144 400 437 656 3489
Fragments arranged by size:
3489 1412 853 819 711 696 656 597
537 437 431 400 348 159 144
BsrBI CCG'CTC
Cuts at: 1394 1823 2158 2752 3433 4840 7240 7531 8014
Size: 429 335 594 681 1407 2400 291 483
Cuts at: 8014 9576 1394
Size: 1562 3507
Fragments arranged by size:
3507 2400 1562 1407 681 594 483 429
335 291
BsrDI GCAATG_nn'
Cuts at: 65 1248 3306 10184 10358 65
Size: 1183 2058 6878 174 1396
Fragments arranged by size:
6878 2058 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2649 4293 6078 8512 10333 10520 1700
Size: 110 839 1644 1785 2434 1821 187 2869
Fragments arranged by size:
2869 2434 1821 1785 1644 839 187 110
BsrGI T'GTAC_A
Cuts at: 96 2386 3144 4554 96
Size: 2290 758 1410 7231
Fragments arranged by size:
7231 2290 1410 758
BssHII G'CGCG_C
Cuts at: 1925 4118 1925
Size: 2193 9496
BssSI C'ACGA_G
Cuts at: 3335 9749 10761 3335
Size: 6414 1012 4263
Fragments arranged by size:
6414 4263 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2337 3025 3550 4480 4534 5140 5152 5302
Size: 1543 688 525 930 54 606 12 150
Cuts at: 5302 6541 7666 8539 794
Size: 1239 1125 873 3944
Fragments arranged by size:
3944 1543 1239 1125 930 873 688 606
525 150 54 12
BstBI TT'CG_AA
Cuts at: 10493 10493
Size: 11689
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 11689
BstXI CCAn_nnnn'nTGG
Cuts at: 4744 8674 4744
Size: 3930 7759
BstYI r'GATC_y
Cuts at: 1972 3978 4089 5209 5622 6837 7428 7941 9019
Size: 2006 111 1120 413 1215 591 513 1078
Cuts at: 9019 9386 9771 9788 11240 11326 11338 11684 1972
Size: 367 385 17 1452 86 12 346 1977
Fragments arranged by size:
2006 1977 1452 1215 1120 1078 591 513
413 385 367 346 111 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 6972 6972
Size: 11689
BtgI C'CryG_G
Cuts at: 513 1065 3051 4063 4390 6195 6599 8175 8448
Size: 552 1986 1012 327 1805 404 1576 273
Cuts at: 8448 8519 8527 8695 8809 513
Size: 71 8 168 114 3393
Fragments arranged by size:
3393 1986 1805 1576 1012 552 404 327
273 168 114 71 8
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3718 4269 4960 7291 487
Size: 683 56 2492 551 691 2331 4885
Fragments arranged by size:
4885 2492 2331 691 683 551 56
BtsI GCAGTG_nn'
Cuts at: 2335 2862 3590 3860 6569 7121 9205 9999 10019
Size: 527 728 270 2709 552 2084 794 20
Cuts at: 10019 2335
Size: 4005
Fragments arranged by size:
4005 2709 2084 794 728 552 527 270
20
ClaI AT'CG_AT
Cuts at: 1554 6000 1554
Size: 4446 7243
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2005 2040 4914 4949 8206 8241 529
Size: 35 1441 35 2874 35 3257 35 3977
Fragments arranged by size:
3977 3257 2874 1441 35 35 35 35
DraI TTT'AAA
Cuts at: 8993 8993
Size: 11689
DraIII CAC_nnn'GTG
Cuts at: 4809 7513 8125 4809
Size: 2704 612 8373
Fragments arranged by size:
8373 2704 612
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2492 3821 4655 4831 10696 809
Size: 507 1176 1329 834 176 5865 1802
Fragments arranged by size:
5865 1802 1329 1176 834 507 176
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2013 4617 4756
Size: 54 1425 50 275 18 183 2604 139
Cuts at: 4756 5016 6723 6913 6933 7299 7383 7494 7578
Size: 260 1707 190 20 366 84 111 84
Cuts at: 7578 8100 8178 8328 9136 10025 11654 8
Size: 522 78 150 808 889 1629 43
Fragments arranged by size:
2604 1707 1629 1425 889 808 522 366
275 260 190 183 150 139 111 84
84 78 54 50 43 20 18
EagI C'GGCC_G
Cuts at: 1537 1812 6723 6913 7494 9136 1537
Size: 275 4911 190 581 1642 4090
Fragments arranged by size:
4911 4090 1642 581 275 190
EarI CTCTTCn'nnn_
Cuts at: 1353 3608 4431 6846 7038 7150 7188 8352 8810
Size: 2255 823 2415 192 112 38 1164 458
Cuts at: 8810 8954 9619 1353
Size: 144 665 3423
Fragments arranged by size:
3423 2415 2255 1164 823 665 458 192
144 112 38
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3904 4529 6662 7055 7727 10276
Size: 1064 138 2315 625 2133 393 672 2549
Cuts at: 10276 10650 10796 387
Size: 374 146 1280
Fragments arranged by size:
2549 2315 2133 1280 1064 672 625 393
374 146 138
EcoICRI GAG'CTC
Cuts at: 3244 4060 4489 5860 6144 6696 6817 7357 7537
Size: 816 429 1371 284 552 121 540 180
Cuts at: 7537 3244
Size: 7396
Fragments arranged by size:
7396 1371 816 552 540 429 284 180
121
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2300 2313 2348 2363 2414 2587 2699
Size: 393 668 13 35 15 51 173 112
Cuts at: 2699 2726 3019 3221 3467 3488 3800 3923 4103
Size: 27 293 202 246 21 312 123 180
Cuts at: 4103 4205 4208 4289 4391 4400 4430 4664 4673
Size: 102 3 81 102 9 30 234 9
Cuts at: 4673 5024 5066 5453 5565 5828 5846 6287 6893
Size: 351 42 387 112 263 18 441 606
Cuts at: 6893 6980 7049 7082 7130 7373 7714 7864 7913
Size: 87 69 33 48 243 341 150 49
Cuts at: 7913 8051 8228 8295 8597 9752 10349 11136 11404
Size: 138 177 67 302 1155 597 787 268
Cuts at: 11404 1239
Size: 1524
Fragments arranged by size:
1524 1155 787 668 606 597 441 393
387 351 341 312 302 293 268 263
246 243 234 202 180 177 173 150
138 123 112 112 102 102 87 81
69 67 51 49 48 42 35 33
30 27 21 18 15 13 9 9
3
EcoNI CCTnn'n_nnAGG
Cuts at: 3458 4685 5285 6703 8462 3458
Size: 1227 600 1418 1759 6685
Fragments arranged by size:
6685 1759 1418 1227 600
EcoO109I rG'GnC_Cy
Cuts at: 2130 2245 3186 3451 4192 4342 4477 4648 4960
Size: 115 941 265 741 150 135 171 312
Cuts at: 4960 5137 5437 6232 6664 6949 8467 8582 8607
Size: 177 300 795 432 285 1518 115 25
Cuts at: 8607 2130
Size: 5212
Fragments arranged by size:
5212 1518 941 795 741 432 312 300
285 265 177 171 150 135 115 115
25
EcoRI G'AATT_C
Cuts at: 8998 9376 8998
Size: 378 11311
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2635 3116 8785 2635
Size: 481 5669 5539
Fragments arranged by size:
5669 5539 481
FspI TGC'GCA
Cuts at: 2330 10195 2330
Size: 7865 3824
FspAI rTGC'GCAy
Cuts at: 2330 2330
Size: 11689
HaeII r_GCGC'y
Cuts at: 1678 2734 3283 3334 3568 6195 6679 6861 7667
Size: 1056 549 51 234 2627 484 182 806
Cuts at: 7667 7690 8197 10836 1678
Size: 23 507 2639 2531
Fragments arranged by size:
2639 2627 2531 1056 806 549 507 484
234 182 51 23
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2093 2125 2780 2812 3634
Size: 32 135 32 521 32 655 32 822
Cuts at: 3634 3666 5039 5071 7736 7768 7796 7828 8260
Size: 32 1373 32 2665 32 28 32 432
Cuts at: 8260 8292 8327 8359 8712 8744 8819 8851 10330
Size: 32 35 32 353 32 75 32 1479
Cuts at: 10330 10362 10404 10436 1373
Size: 32 42 32 2626
Fragments arranged by size:
2665 2626 1479 1373 822 655 521 432
353 135 75 42 35 32 32 32
32 32 32 32 32 32 32 32
32 32 32 28
HincII GTy'rAC
Cuts at: 669 1657 1938 9033 9279 669
Size: 988 281 7095 246 3079
Fragments arranged by size:
7095 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 11689
HpaI GTT'AAC
Cuts at: 9279 9279
Size: 11689
KasI G'GCGC_C
Cuts at: 3564 6857 7686 3564
Size: 3293 829 7567
Fragments arranged by size:
7567 3293 829
KpnI G_GTAC'C
Cuts at: 3805 9014 3805
Size: 5209 6480
MfeI C'AATT_G
Cuts at: 9288 9288
Size: 11689
MluI A'CGCG_T
Cuts at: 11565 11565
Size: 11689
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3666 4815 6171 8625 10803 10987 1305
Size: 2361 1149 1356 2454 2178 184 2007
Fragments arranged by size:
2454 2361 2178 2007 1356 1149 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4758 6935 7580 8102 10
Size: 54 1425 343 2926 2177 645 522 3597
Fragments arranged by size:
3597 2926 2177 1425 645 522 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2583 2810 6866 7370 8412 9647 10006
Size: 648 1417 227 4056 504 1042 1235 359
Cuts at: 10006 10165 518
Size: 159 2042
Fragments arranged by size:
4056 2042 1417 1235 1042 648 504 359
227 159
MspA1I CmG'CkG
Cuts at: 1606 2070 2607 2737 2928 3025 3371 3884 4186
Size: 464 537 130 191 97 346 513 302
Cuts at: 4186 5065 5107 5191 5428 5755 6019 6208 6424
Size: 879 42 84 237 327 264 189 216
Cuts at: 6424 6601 6634 6722 6931 7378 7918 8007 8106
Size: 177 33 88 209 447 540 89 99
Cuts at: 8106 8184 8521 9105 9782 10930 11175 1606
Size: 78 337 584 677 1148 245 2120
Fragments arranged by size:
2120 1148 879 677 584 540 537 513
464 447 346 337 327 302 264 245
237 216 209 191 189 177 130 99
97 89 88 84 78 42 33
NaeI GCC'GGC
Cuts at: 8514 8514
Size: 11689
NarI GG'CG_CC
Cuts at: 3565 6858 7687 3565
Size: 3293 829 7567
Fragments arranged by size:
7567 3293 829
NcoI C'CATG_G
Cuts at: 513 1065 6195 8527 8695 8809 513
Size: 552 5130 2332 168 114 3393
Fragments arranged by size:
5130 3393 2332 552 168 114
NdeI CA'TA_TG
Cuts at: 387 7600 387
Size: 7213 4476
NgoMIV G'CCGG_C
Cuts at: 8512 8512
Size: 11689
NheI G'CTAG_C
Cuts at: 1052 2932 1052
Size: 1880 9809
NotI GC'GGCC_GC
Cuts at: 9136 9136
Size: 11689
NspI r_CATG'y
Cuts at: 1132 1229 2266 3616 5272 5315 7072 7375 8026
Size: 97 1037 1350 1656 43 1757 303 651
Cuts at: 8026 8726 9047 10592 1132
Size: 700 321 1545 2229
Fragments arranged by size:
2229 1757 1656 1545 1350 1037 700 651
321 303 97 43
PasI CC'CwG_GG
Cuts at: 1348 4345 4423 5461 6946 7800 8680 1348
Size: 2997 78 1038 1485 854 880 4357
Fragments arranged by size:
4357 2997 1485 1038 880 854 78
PciI A'CATG_T
Cuts at: 7068 10588 7068
Size: 3520 8169
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2341 2437 4501 4908 5431 6064 7546 7576
Size: 1233 96 2064 407 523 633 1482 30
Cuts at: 7576 7702 1108
Size: 126 5095
Fragments arranged by size:
5095 2064 1482 1233 633 523 407 126
96 30
PfoI T'CCnGG_A
Cuts at: 2132 3116 3606 3828 4095 4221 4368 4680 5514
Size: 984 490 222 267 126 147 312 834
Cuts at: 5514 6384 6393 7200 7350 7569 7754 7970 8609
Size: 870 9 807 150 219 185 216 639
Cuts at: 8609 2132
Size: 5212
Fragments arranged by size:
5212 984 870 834 807 639 490 312
267 222 219 216 185 150 147 126
9
PmeI GTTT'AAAC
Cuts at: 8993 8993
Size: 11689
PmlI CAC'GTG
Cuts at: 7177 7177
Size: 11689
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2390 2422 6365 6397 8356 8388 9758 9790 11271
Size: 32 3943 32 1959 32 1370 32 1481
Cuts at: 11271 11303 2390
Size: 32 2776
Fragments arranged by size:
3943 2776 1959 1481 1370 32 32 32
32 32
PpuMI rG'GwC_Cy
Cuts at: 2130 2245 3451 4192 4648 4960 6232 6664 8467
Size: 115 1206 741 456 312 1272 432 1803
Cuts at: 8467 8607 2130
Size: 140 5212
Fragments arranged by size:
5212 1803 1272 1206 741 456 432 312
140 115
PshAI GACnn'nnGTC
Cuts at: 1613 3011 4521 6242 1613
Size: 1398 1510 1721 7060
Fragments arranged by size:
7060 1721 1510 1398
PsiI TTA'TAA
Cuts at: 9259 9259
Size: 11689
PspOMI G'GGCC_C
Cuts at: 4341 6942 8582 4341
Size: 2601 1640 7448
Fragments arranged by size:
7448 2601 1640
PspXI vC'TCGA_Gb
Cuts at: 2211 2211
Size: 11689
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 4547 4579 7064 7096 8606 8638 4547
Size: 32 2485 32 1510 32 7598
Fragments arranged by size:
7598 2485 1510 32 32 32
PstI C_TGCA'G
Cuts at: 830 3030 3546 3558 3597 4476 4539 5145 5157
Size: 2200 516 12 39 879 63 606 12
Cuts at: 5157 5196 5208 5307 5614 6099 6450 6576 6621
Size: 39 12 99 307 485 351 126 45
Cuts at: 6621 6693 6738 6964 7593 7917 7959 8385 8847
Size: 72 45 226 629 324 42 426 462
Cuts at: 8847 9041 830
Size: 194 3478
Fragments arranged by size:
3478 2200 879 629 606 516 485 462
426 351 324 307 226 194 126 99
72 63 45 45 42 39 39 12
12 12
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 11689
PvuII CAG'CTG
Cuts at: 2607 2737 3025 3371 3884 4186 5065 5191 5755
Size: 130 288 346 513 302 879 126 564
Cuts at: 5755 6019 6208 6424 6634 6931 7378 8184 2607
Size: 264 189 216 210 297 447 806 6112
Fragments arranged by size:
6112 879 806 564 513 447 346 302
297 288 264 216 210 189 130 126
SacI G_AGCT'C
Cuts at: 3246 4062 4491 5862 6146 6698 6819 7359 7539
Size: 816 429 1371 284 552 121 540 180
Cuts at: 7539 3246
Size: 7396
Fragments arranged by size:
7396 1371 816 552 540 429 284 180
121
SacII CC_GC'GG
Cuts at: 6602 8522 6602
Size: 1920 9769
SalI G'TCGA_C
Cuts at: 1936 9031 1936
Size: 7095 4594
SapI GCTCTTCn'nnn_
Cuts at: 3608 7038 8810 3608
Size: 3430 1772 6487
Fragments arranged by size:
6487 3430 1772
SbfI CC_TGCA'GG
Cuts at: 3546 5157 5208 9041 3546
Size: 1611 51 3833 6194
Fragments arranged by size:
6194 3833 1611 51
ScaI AGT'ACT
Cuts at: 6770 9937 11649 6770
Size: 3167 1712 6810
Fragments arranged by size:
6810 3167 1712
SexAI A'CCwGG_T
Cuts at: 4805 4805
Size: 11689
SfcI C'TryA_G
Cuts at: 826 1045 3026 3542 3554 3593 3704 4472 4535
Size: 219 1981 516 12 39 111 768 63
Cuts at: 4535 5141 5153 5192 5204 5303 5610 5831 6095
Size: 606 12 39 12 99 307 221 264
Cuts at: 6095 6446 6572 6617 6689 6734 6960 7589 7913
Size: 351 126 45 72 45 226 629 324
Cuts at: 7913 7955 8381 8843 9037 10172 10853 11044 826
Size: 42 426 462 194 1135 681 191 1471
Fragments arranged by size:
1981 1471 1135 768 681 629 606 516
462 426 351 324 307 264 226 221
219 194 191 126 111 99 72 63
45 45 42 39 39 12 12 12
SfiI GGCCn_nnn'nGGCC
Cuts at: 4340 5798 6941 4340
Size: 1458 1143 9088
Fragments arranged by size:
9088 1458 1143
SfoI GGC'GCC
Cuts at: 3566 6859 7688 3566
Size: 3293 829 7567
Fragments arranged by size:
7567 3293 829
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 11689
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 11689
SmaI CCC'GGG
Cuts at: 1872 6225 7214 8349 9016 1872
Size: 4353 989 1135 667 4545
Fragments arranged by size:
4545 4353 1135 989 667
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2211 2372 3575 5677 6364 6668
Size: 197 926 268 161 1203 2102 687 304
Cuts at: 6668 7088 8047 8225 8277 9793 10694 10956 820
Size: 420 959 178 52 1516 901 262 1553
Fragments arranged by size:
2102 1553 1516 1203 959 926 901 687
420 304 268 262 197 178 161 52
SnaBI TAC'GTA
Cuts at: 493 9480 493
Size: 8987 2702
SpeI A'CTAG_T
Cuts at: 152 3166 152
Size: 3014 8675
SphI G_CATG'C
Cuts at: 1132 2266 5315 7375 9047 1132
Size: 1134 3049 2060 1672 3774
Fragments arranged by size:
3774 3049 2060 1672 1134
SspI AAT'ATT
Cuts at: 5 52 9613 5
Size: 47 9561 2081
Fragments arranged by size:
9561 2081 47
StuI AGG'CCT
Cuts at: 1517 2144 3032 4046 5198 5228 5801 6797 7931
Size: 627 888 1014 1152 30 573 996 1134
Cuts at: 7931 1517
Size: 5275
Fragments arranged by size:
5275 1152 1134 1014 996 888 627 573
30
StyI C'CwwG_G
Cuts at: 513 1065 2145 2316 2529 3742 5389 5506 6195
Size: 552 1080 171 213 1213 1647 117 689
Cuts at: 6195 6403 6843 8527 8695 8809 9128 513
Size: 208 440 1684 168 114 319 3074
Fragments arranged by size:
3074 1684 1647 1213 1080 689 552 440
319 213 208 171 168 117 114
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2182 3425 4776 5258 6472 7115 7538
Size: 1255 602 1243 1351 482 1214 643 423
Cuts at: 7538 9716 9733 9886 10071 10516 325
Size: 2178 17 153 185 445 1498
Fragments arranged by size:
2178 1498 1351 1255 1243 1214 643 602
482 445 423 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2386 2922 3144
Size: 274 80 33 51 1443 409 536 222
Cuts at: 3144 4554 6768 8622 9935 11647 96
Size: 1410 2214 1854 1313 1712 138
Fragments arranged by size:
2214 1854 1712 1443 1410 1313 536 409
274 222 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2819 2851 5114 5146 8109 8141 11649 11681 2819
Size: 32 2263 32 2963 32 3508 32 2827
Fragments arranged by size:
3508 2963 2827 2263 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 11689
XbaI T'CTAG_A
Cuts at: 9025 9025
Size: 11689
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3350 4103 4342 4948 3350
Size: 753 239 606 10091
Fragments arranged by size:
10091 753 606 239
XhoI C'TCGA_G
Cuts at: 1943 2211 3575 8047 1943
Size: 268 1364 4472 5585
Fragments arranged by size:
5585 4472 1364 268
XmaI C'CCGG_G
Cuts at: 1870 6223 7212 8347 9014 1870
Size: 4353 989 1135 667 4545
Fragments arranged by size:
4545 4353 1135 989 667
XmnI GAAnn'nnTTC
Cuts at: 2166 3602 3929 4238 4440 6380 7259 8963 9818
Size: 1436 327 309 202 1940 879 1704 855
Cuts at: 9818 2166
Size: 4037
Fragments arranged by size:
4037 1940 1704 1436 879 855 327 309
202
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 9497 276
Size: 53 83 186 936 7963 2468
Fragments arranged by size:
7963 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII
SacI SacII SalI SapI SbfI ScaI SexAI SfcI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI BaeI BfrBI BsiWI BstZ17I EcoRV FseI NruI
NsiI PacI RsrII SanDI SrfI SwaI