Restriction map for
pFN21AB4391
(Circular) (Six-base) MAPSORT of: pFN21AB4391.seq Check: 5079 from: 1 to: 6265
pFN21AB4391 6265 bp
With 182 enzymes: SgfI *
February 27, 2009 14:33 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4075 278
Size: 53 83 186 936 2539 2468
Fragments arranged by size:
2539 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3608 1937
Size: 1671 4594
Acc65I G'GTAC_C
Cuts at: 3586 3586
Size: 6265
AclI AA'CG_TT
Cuts at: 1563 4392 4765 1563
Size: 2829 373 3063
Fragments arranged by size:
3063 2829 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2510 2816 4328 5712 5980 1632
Size: 878 306 1512 1384 268 1917
Fragments arranged by size:
1917 1512 1384 878 306 268
AfeI AGC'GCT
Cuts at: 3059 3059
Size: 6265
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6068
AflIII A'CryG_T
Cuts at: 1203 2504 5164 6141 1203
Size: 1301 2660 977 1327
Fragments arranged by size:
2660 1327 1301 977
AgeI A'CCGG_T
Cuts at: 5096 5096
Size: 6265
AhdI GACnn_n'nnGTC
Cuts at: 4994 4994
Size: 6265
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6265
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6265
AlwNI CAG_nnn'CTG
Cuts at: 2034 4008 5580 2034
Size: 1974 1572 2719
Fragments arranged by size:
2719 1974 1572
ApaLI G'TGCA_C
Cuts at: 3386 4322 5478 3386
Size: 936 1156 4173
Fragments arranged by size:
4173 1156 936
ApoI r'AATT_y
Cuts at: 1493 2491 2792 2878 3290 3574 3800 3952 6061
Size: 998 301 86 412 284 226 152 2109
Cuts at: 6061 1493
Size: 1697
Fragments arranged by size:
2109 1697 998 412 301 284 226 152
86
AseI AT'TA_AT
Cuts at: 160 1032 4819 160
Size: 872 3787 1606
Fragments arranged by size:
3787 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6265
AvaI C'yCGr_G
Cuts at: 1870 1943 3590 1870
Size: 73 1647 4545
Fragments arranged by size:
4545 1647 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3270 3303 3270
Size: 33 6232
BamHI G'GATC_C
Cuts at: 3595 3962 3595
Size: 367 5898
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3586 5041 618
Size: 325 217 627 1799 1455 1842
Fragments arranged by size:
1842 1799 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2027 3584 1391
Size: 636 1557 4072
Fragments arranged by size:
4072 1557 636
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3098 928
Size: 971 1199 4095
Fragments arranged by size:
4095 1199 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4456 4490 1903
Size: 34 2519 34 3678
Fragments arranged by size:
3678 2519 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4157 5367 4157
Size: 1210 5055
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6265
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4876 136
Size: 107 122 71 631 605 3204 1525
Fragments arranged by size:
3204 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6260 6260
Size: 6265
BlpI GC'TnA_GC
Cuts at: 2860 3682 2860
Size: 822 5443
Bme1580I G_kGCm'C
Cuts at: 1790 3390 4326 5482 1790
Size: 1600 936 1156 2573
Fragments arranged by size:
2573 1600 1156 936
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6265
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3996 4954 442
Size: 416 53 83 399 24 2579 958 1753
Fragments arranged by size:
2579 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6265
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2972 2972
Size: 6265
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2011 4925 1239
Size: 772 2914 2579
Fragments arranged by size:
2914 2579 772
Bpu10I CC'TnA_GC
Cuts at: 2021 2021
Size: 6265
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4390 5255 5553 4390
Size: 865 298 5102
Fragments arranged by size:
5102 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4928 882
Size: 617 3429 2219
Fragments arranged by size:
3429 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3286 3529 4056 493
Size: 711 2082 243 527 2702
Fragments arranged by size:
2702 2082 711 527 243
BsaBI GATnn'nnATC
Cuts at: 6259 6259
Size: 6265
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2784 4072 4454 5089
Size: 53 83 186 936 1251 1288 382 635
Cuts at: 5089 275
Size: 1451
Fragments arranged by size:
1451 1288 1251 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2044 4698 5096 5370 5517 5928 2044
Size: 2654 398 274 147 411 2381
Fragments arranged by size:
2654 2381 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2009 2039 1178
Size: 30 165 30 606 30 5404
Fragments arranged by size:
5404 606 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3503 1186
Size: 2317 3948
BseYI C'CCAG_C
Cuts at: 2326 5468 5964 2326
Size: 3142 496 2627
Fragments arranged by size:
3142 2627 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5692
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3715 4476 5504 1540
Size: 275 186 1714 761 1028 2301
Fragments arranged by size:
2301 1714 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2027 3390 4326 4411 5482 2027
Size: 1363 936 85 1071 2810
Fragments arranged by size:
2810 1363 1071 936 85
BsmI GAATG_Cn'
Cuts at: 2521 2952 3776 3869 2521
Size: 431 824 93 4917
Fragments arranged by size:
4917 824 431 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2027 3390 3584 4326 4411 5482 1391
Size: 399 237 1363 194 742 85 1071 2174
Fragments arranged by size:
2174 1363 1071 742 399 237 194 85
BspEI T'CCGG_A
Cuts at: 2044 5928 2044
Size: 3884 2381
BspHI T'CATG_A
Cuts at: 2511 4154 5884 2511
Size: 1643 1730 2892
Fragments arranged by size:
2892 1730 1643
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3620 844
Size: 2776 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2859 4152 1394
Size: 429 1036 1293 3507
Fragments arranged by size:
3507 1293 1036 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2037 4760 4934 65
Size: 1183 789 2723 174 1396
Fragments arranged by size:
2723 1396 1183 789 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2100 4909 5096 1700
Size: 110 290 2809 187 2869
Fragments arranged by size:
2869 2809 290 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6265
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6265
BssSI C'ACGA_G
Cuts at: 4325 5337 4325
Size: 1012 5253
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2097 794
Size: 1303 4962
BstBI TT'CG_AA
Cuts at: 5069 5069
Size: 6265
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6265
BstYI r'GATC_y
Cuts at: 1972 3595 3962 4347 4364 5816 5902 5914 6260
Size: 1623 367 385 17 1452 86 12 346
Cuts at: 6260 1972
Size: 1977
Fragments arranged by size:
1977 1623 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2580 3391 513
Size: 552 939 576 811 3387
Fragments arranged by size:
3387 939 811 576 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5526
Fragments arranged by size:
5526 683 56
BtsI GCAGTG_nn'
Cuts at: 2463 3781 4575 4595 2463
Size: 1318 794 20 4133
Fragments arranged by size:
4133 1318 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6265
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6230
DraI TTT'AAA
Cuts at: 3569 3569
Size: 6265
DraIII CAC_nnn'GTG
Cuts at: 2684 2684
Size: 6265
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5272 809
Size: 507 3956 1802
Fragments arranged by size:
3956 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2167 3712 4601
Size: 54 1425 50 275 18 337 1545 889
Cuts at: 4601 6230 8
Size: 1629 43
Fragments arranged by size:
1629 1545 1425 889 337 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3712 1537
Size: 275 1900 4090
Fragments arranged by size:
4090 1900 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2658 4195 1353
Size: 1305 1537 3423
Fragments arranged by size:
3423 1537 1305
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2629 4852 5226 5372 387
Size: 1064 138 1040 2223 374 146 1280
Fragments arranged by size:
2223 1280 1064 1040 374 146 138
EcoICRI GAG'CTC
Cuts at: 2025 2025
Size: 6265
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2011 2510 2816 4328 4925 5712 5980
Size: 393 379 499 306 1512 597 787 268
Cuts at: 5980 1239
Size: 1524
Fragments arranged by size:
1524 1512 787 597 499 393 379 306
268
EcoRI G'AATT_C
Cuts at: 2792 2878 3574 3952 2792
Size: 86 696 378 5105
Fragments arranged by size:
5105 696 378 86
FspI TGC'GCA
Cuts at: 4771 4771
Size: 6265
HaeII r_GCGC'y
Cuts at: 1678 3061 5412 1678
Size: 1383 2351 2531
Fragments arranged by size:
2531 2351 1383
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4906 4938 4980 5012 1373
Size: 32 135 32 3334 32 42 32 2626
Fragments arranged by size:
3334 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2971 3609 3855 669
Size: 988 281 1033 638 246 3079
Fragments arranged by size:
3079 1033 988 638 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6265
HpaI GTT'AAC
Cuts at: 2971 3855 2971
Size: 884 5381
KpnI G_GTAC'C
Cuts at: 3590 3590
Size: 6265
MfeI C'AATT_G
Cuts at: 3864 3864
Size: 6265
MluI A'CGCG_T
Cuts at: 6141 6141
Size: 6265
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2130 5379 5563 1305
Size: 825 3249 184 2007
Fragments arranged by size:
3249 2007 825 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2169 10
Size: 54 1425 343 337 4106
Fragments arranged by size:
4106 1425 343 337 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3459 4223 4582 4741 518
Size: 648 2293 764 359 159 2042
Fragments arranged by size:
2293 2042 764 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2387 3377 3447 3681 4358 5506 5751 1606
Size: 781 990 70 234 677 1148 245 2120
Fragments arranged by size:
2120 1148 990 781 677 245 234 70
NcoI C'CATG_G
Cuts at: 513 1065 2004 3391 513
Size: 552 939 1387 3387
Fragments arranged by size:
3387 1387 939 552
NdeI CA'TA_TG
Cuts at: 387 2981 387
Size: 2594 3671
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6265
NotI GC'GGCC_GC
Cuts at: 3712 3712
Size: 6265
NspI r_CATG'y
Cuts at: 1132 1229 2508 3623 5168 1132
Size: 97 1279 1115 1545 2229
Fragments arranged by size:
2229 1545 1279 1115 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6265
PciI A'CATG_T
Cuts at: 2504 5164 2504
Size: 2660 3605
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6265
PmeI GTTT'AAAC
Cuts at: 3569 3569
Size: 6265
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4334 4366 5847 5879 4334
Size: 32 1481 32 4720
Fragments arranged by size:
4720 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6265
PsiI TTA'TAA
Cuts at: 3835 3835
Size: 6265
PstI C_TGCA'G
Cuts at: 830 2093 2874 3617 830
Size: 1263 781 743 3478
Fragments arranged by size:
3478 1263 781 743
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6265
PvuII CAG'CTG
Cuts at: 2387 2387
Size: 6265
SacI G_AGCT'C
Cuts at: 2027 2027
Size: 6265
SalI G'TCGA_C
Cuts at: 1936 3607 1936
Size: 1671 4594
SbfI CC_TGCA'GG
Cuts at: 3617 3617
Size: 6265
ScaI AGT'ACT
Cuts at: 3469 4513 6225 3469
Size: 1044 1712 3509
Fragments arranged by size:
3509 1712 1044
SfcI C'TryA_G
Cuts at: 826 1045 2089 2275 2870 3613 4748 5429 5620
Size: 219 1044 186 595 743 1135 681 191
Cuts at: 5620 826
Size: 1471
Fragments arranged by size:
1471 1135 1044 743 681 595 219 191
186
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6265
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6265
SmaI CCC'GGG
Cuts at: 1872 3592 1872
Size: 1720 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4369 5270 5532 820
Size: 197 926 2426 901 262 1553
Fragments arranged by size:
2426 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3286 4056 493
Size: 2793 770 2702
Fragments arranged by size:
2793 2702 770
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6265
SphI G_CATG'C
Cuts at: 1132 3623 1132
Size: 2491 3774
SspI AAT'ATT
Cuts at: 5 52 4189 5
Size: 47 4137 2081
Fragments arranged by size:
4137 2081 47
StuI AGG'CCT
Cuts at: 1517 2484 3306 3512 1517
Size: 967 822 206 4270
Fragments arranged by size:
4270 967 822 206
StyI C'CwwG_G
Cuts at: 513 1065 2004 2117 2635 3216 3391 3704 513
Size: 552 939 113 518 581 175 313 3074
Fragments arranged by size:
3074 939 581 552 518 313 175 113
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4292 4309 4462 4647 5092 325
Size: 1255 2712 17 153 185 445 1498
Fragments arranged by size:
2712 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2279 3467 4511
Size: 274 80 33 51 1443 302 1188 1044
Cuts at: 4511 6223 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1188 1044 302 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2009 2041 2145 2177 3380 3412 6225 6257 2009
Size: 32 104 32 1203 32 2813 32 2017
Fragments arranged by size:
2813 2017 1203 104 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6265
XbaI T'CTAG_A
Cuts at: 2996 3601 2996
Size: 605 5660
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2329 3545 2329
Size: 1216 5049
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6265
XmaI C'CCGG_G
Cuts at: 1870 3590 1870
Size: 1720 4545
XmnI GAAnn'nnTTC
Cuts at: 4394 4394
Size: 6265
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4073 276
Size: 53 83 186 936 2539 2468
Fragments arranged by size:
2539 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PstI
PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BbeI BbvCI BfrBI
BsiWI BsmBI BstXI BstZ17I Bsu36I EcoNI EcoO109I EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI
RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI