Restriction map for
pFN21AB4387
(Circular) (Six-base) MAPSORT of: pFN21AB4387.seq Check: 893 from: 1 to: 7279
pFN21AB4387 7279 bp
With 182 enzymes: SgfI *
October 20, 2009 10:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2213 2489 3101 2213
Size: 276 612 6391
Fragments arranged by size:
6391 612 276
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5089 278
Size: 53 83 186 936 3553 2468
Fragments arranged by size:
3553 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4622 1937
Size: 2685 4594
Acc65I G'GTAC_C
Cuts at: 4600 4600
Size: 7279
AclI AA'CG_TT
Cuts at: 1563 3533 5406 5779 1563
Size: 1970 1873 373 3063
Fragments arranged by size:
3063 1970 1873 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2206 5342 6726 6994 1632
Size: 574 3136 1384 268 1917
Fragments arranged by size:
3136 1917 1384 574 268
AfeI AGC'GCT
Cuts at: 2152 2552 2152
Size: 400 6879
AflII C'TTAA_G
Cuts at: 820 1017 2999 4448 820
Size: 197 1982 1449 3651
Fragments arranged by size:
3651 1982 1449 197
AflIII A'CryG_T
Cuts at: 1203 2568 6178 7155 1203
Size: 1365 3610 977 1327
Fragments arranged by size:
3610 1365 1327 977
AgeI A'CCGG_T
Cuts at: 6110 6110
Size: 7279
AhdI GACnn_n'nnGTC
Cuts at: 6008 6008
Size: 7279
AleI CACnn'nnGTG
Cuts at: 1166 3308 4286 1166
Size: 2142 978 4159
Fragments arranged by size:
4159 2142 978
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7279
AlwNI CAG_nnn'CTG
Cuts at: 4042 4148 5022 6594 4042
Size: 106 874 1572 4727
Fragments arranged by size:
4727 1572 874 106
ApaI G_GGCC'C
Cuts at: 2013 2013
Size: 7279
ApaLI G'TGCA_C
Cuts at: 5336 6492 5336
Size: 1156 6123
ApoI r'AATT_y
Cuts at: 1493 2723 4588 4814 4966 7075 1493
Size: 1230 1865 226 152 2109 1697
Fragments arranged by size:
2109 1865 1697 1230 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5833 160
Size: 872 4801 1606
Fragments arranged by size:
4801 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7279
AvaI C'yCGr_G
Cuts at: 1870 1943 4604 1870
Size: 73 2661 4545
Fragments arranged by size:
4545 2661 73
BamHI G'GATC_C
Cuts at: 3082 3453 3577 4609 4976 3082
Size: 371 124 1032 367 5385
Fragments arranged by size:
5385 1032 371 367 124
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4600 6055 618
Size: 325 217 627 2813 1455 1842
Fragments arranged by size:
2813 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2013 3194 3382 4281 4372 4598 1391
Size: 622 1181 188 899 91 226 4072
Fragments arranged by size:
4072 1181 899 622 226 188 91
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3797 4396 4414 4499 928
Size: 971 1898 599 18 85 3708
Fragments arranged by size:
3708 1898 971 599 85 18
BbvCI CC'TCA_GC
Cuts at: 4149 4149
Size: 7279
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5470 5504 1903
Size: 34 3533 34 3678
Fragments arranged by size:
3678 3533 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3502 5171 6381 3502
Size: 1669 1210 4400
Fragments arranged by size:
4400 1669 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7279
BfrBI ATG'CAT
Cuts at: 2121 2871 2121
Size: 750 6529
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2166 2622 3030
Size: 107 122 71 631 605 494 456 408
Cuts at: 3030 3182 4104 5890 136
Size: 152 922 1786 1525
Fragments arranged by size:
1786 1525 922 631 605 494 456 408
152 122 107 71
BglII A'GATC_T
Cuts at: 3709 7274 3709
Size: 3565 3714
BlpI GC'TnA_GC
Cuts at: 4696 4696
Size: 7279
Bme1580I G_kGCm'C
Cuts at: 1790 2013 3026 3494 5340 6496 1790
Size: 223 1013 468 1846 1156 2573
Fragments arranged by size:
2573 1846 1156 1013 468 223
BmgBI CAC'GTC
Cuts at: 1318 2190 2328 2986 4573 1318
Size: 872 138 658 1587 4024
Fragments arranged by size:
4024 1587 872 658 138
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3619 3742 4350
Size: 416 53 83 399 24 2202 123 608
Cuts at: 4350 5010 5968 442
Size: 660 958 1753
Fragments arranged by size:
2202 1753 958 660 608 416 399 123
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7279
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3568 3568
Size: 7279
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2284 2623 3137 4583 5939 1239
Size: 1045 339 514 1446 1356 2579
Fragments arranged by size:
2579 1446 1356 1045 514 339
Bpu10I CC'TnA_GC
Cuts at: 4019 4149 4019
Size: 130 7149
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3745 5404 6269 6567 3745
Size: 1659 865 298 4457
Fragments arranged by size:
4457 1659 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5942 882
Size: 617 4443 2219
Fragments arranged by size:
4443 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2569 5070 493
Size: 711 1365 2501 2702
Fragments arranged by size:
2702 2501 1365 711
BsaBI GATnn'nnATC
Cuts at: 7273 7273
Size: 7279
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3387 5086 5468 6103
Size: 53 83 186 936 1854 1699 382 635
Cuts at: 6103 275
Size: 1451
Fragments arranged by size:
1854 1699 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3249 3786 5712 6110 6384 6531 6942 3249
Size: 537 1926 398 274 147 411 3586
Fragments arranged by size:
3586 1926 537 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2411 2441 3815 3845 1178
Size: 30 165 30 1008 30 1374 30 4612
Fragments arranged by size:
4612 1374 1008 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2039 3704 4005 4144 4388 1186
Size: 853 1665 301 139 244 4077
Fragments arranged by size:
4077 1665 853 301 244 139
BseYI C'CCAG_C
Cuts at: 2490 3048 3366 3981 6482 6978 2490
Size: 558 318 615 2501 496 2791
Fragments arranged by size:
2791 2501 615 558 496 318
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6706
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4729 5490 6518 1540
Size: 275 186 2728 761 1028 2301
Fragments arranged by size:
2728 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2333 2442 2735 4398 5340 5425 6496 2333
Size: 109 293 1663 942 85 1071 3116
Fragments arranged by size:
3116 1663 1071 942 293 109 85
BsmI GAATG_Cn'
Cuts at: 2250 2873 2960 4790 4883 2250
Size: 623 87 1830 93 4646
Fragments arranged by size:
4646 1830 623 93 87
BsmBI CGTCTCn'nnnn_
Cuts at: 2180 3394 2180
Size: 1214 6065
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2013 2333 2442 2735 3026 3194 3382
Size: 399 223 320 109 293 291 168 188
Cuts at: 3382 3494 4281 4372 4398 4598 5340 5425 6496
Size: 112 787 91 26 200 742 85 1071
Cuts at: 6496 1391
Size: 2174
Fragments arranged by size:
2174 1071 787 742 399 320 293 291
223 200 188 168 112 109 91 85
26
BspEI T'CCGG_A
Cuts at: 6942 6942
Size: 7279
BspHI T'CATG_A
Cuts at: 5168 6898 5168
Size: 1730 5549
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2213 2489 3101 4097 4634 844
Size: 1369 276 612 996 537 3489
Fragments arranged by size:
3489 1369 996 612 537 276
BsrBI CCG'CTC
Cuts at: 1394 1823 5166 1394
Size: 429 3343 3507
Fragments arranged by size:
3507 3343 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2998 4030 5774 5948 65
Size: 1183 1750 1032 1744 174 1396
Fragments arranged by size:
1750 1744 1396 1183 1032 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2760 3219 5923 6110 1700
Size: 110 950 459 2704 187 2869
Fragments arranged by size:
2869 2704 950 459 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7279
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7279
BssSI C'ACGA_G
Cuts at: 3224 5339 6351 3224
Size: 2115 1012 4152
Fragments arranged by size:
4152 2115 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7279
BstBI TT'CG_AA
Cuts at: 6083 6083
Size: 7279
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7279
BstXI CCAn_nnnn'nTGG
Cuts at: 3574 3574
Size: 7279
BstYI r'GATC_y
Cuts at: 1972 3082 3453 3577 3709 4609 4976 5361 5378
Size: 1110 371 124 132 900 367 385 17
Cuts at: 5378 6830 6916 6928 7274 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1110 900 385 371 367 346
132 124 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3281 3281
Size: 7279
BtgI C'CryG_G
Cuts at: 513 1065 2004 2055 2752 3382 4284 513
Size: 552 939 51 697 630 902 3508
Fragments arranged by size:
3508 939 902 697 630 552 51
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2157 2286 2814 3181 487
Size: 683 56 931 129 528 367 4585
Fragments arranged by size:
4585 931 683 528 367 129 56
BtsI GCAGTG_nn'
Cuts at: 2076 2611 2771 4164 4795 5589 5609 2076
Size: 535 160 1393 631 794 20 3746
Fragments arranged by size:
3746 1393 794 631 535 160 20
ClaI AT'CG_AT
Cuts at: 1554 3069 3768 1554
Size: 1515 699 5065
Fragments arranged by size:
5065 1515 699
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7244
DraI TTT'AAA
Cuts at: 4583 4583
Size: 7279
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2194 4481 6286 809
Size: 507 878 2287 1805 1802
Fragments arranged by size:
2287 1805 1802 878 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2275 2543 2703
Size: 54 1425 50 275 18 445 268 160
Cuts at: 2703 3312 3721 3837 4116 4161 4726 5615 7244
Size: 609 409 116 279 45 565 889 1629
Cuts at: 7244 8
Size: 43
Fragments arranged by size:
1629 1425 889 609 565 445 409 279
275 268 160 116 54 50 45 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4726 1537
Size: 275 2914 4090
Fragments arranged by size:
4090 2914 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3212 3397 3518 3971 5209 1353
Size: 1859 185 121 453 1238 3423
Fragments arranged by size:
3423 1859 1238 453 185 121
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5866 6240 6386 387
Size: 1064 138 4277 374 146 1280
Fragments arranged by size:
4277 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2206 2284 2623 3137 4583 5342 5939
Size: 393 574 78 339 514 1446 759 597
Cuts at: 5939 6726 6994 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1446 787 759 597 574 514 393
339 268 78
EcoNI CCTnn'n_nnAGG
Cuts at: 4142 4142
Size: 7279
EcoO109I rG'GnC_Cy
Cuts at: 2009 2010 2837 3427 3640 2009
Size: 1 827 590 213 5648
Fragments arranged by size:
5648 827 590 213 1
EcoRI G'AATT_C
Cuts at: 2723 4588 4966 2723
Size: 1865 378 5036
Fragments arranged by size:
5036 1865 378
EcoRV GAT'ATC
Cuts at: 3067 3871 3067
Size: 804 6475
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3814 3814
Size: 7279
FspI TGC'GCA
Cuts at: 5785 5785
Size: 7279
HaeII r_GCGC'y
Cuts at: 1678 2154 2554 2650 6426 1678
Size: 476 400 96 3776 2531
Fragments arranged by size:
3776 2531 476 400 96
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2282 2314 2411 2443 3647
Size: 32 135 32 710 32 97 32 1204
Cuts at: 3647 3679 4291 4323 4543 4575 5920 5952 5994
Size: 32 612 32 220 32 1345 32 42
Cuts at: 5994 6026 1373
Size: 32 2626
Fragments arranged by size:
2626 1345 1204 710 612 220 135 97
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2242 3739 3775 3922 4623 4869
Size: 988 281 304 1497 36 147 701 246
Cuts at: 4869 669
Size: 3079
Fragments arranged by size:
3079 1497 988 701 304 281 246 147
36
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7279
HpaI GTT'AAC
Cuts at: 4869 4869
Size: 7279
KpnI G_GTAC'C
Cuts at: 4604 4604
Size: 7279
MfeI C'AATT_G
Cuts at: 4878 4878
Size: 7279
MluI A'CGCG_T
Cuts at: 7155 7155
Size: 7279
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6393 6577 1305
Size: 5088 184 2007
Fragments arranged by size:
5088 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2277 2705 3314 4118 4163
Size: 54 1425 343 445 428 609 804 45
Cuts at: 4163 10
Size: 3126
Fragments arranged by size:
3126 1425 804 609 445 428 343 54
45
MslI CAynn'nnrTG
Cuts at: 518 1166 3308 3572 4286 5237 5596 5755 518
Size: 648 2142 264 714 951 359 159 2042
Fragments arranged by size:
2142 2042 951 714 648 359 264 159
MspA1I CmG'CkG
Cuts at: 1606 2057 2226 2247 2494 2643 2862 3040 3157
Size: 451 169 21 247 149 219 178 117
Cuts at: 3157 4695 5372 6520 6765 1606
Size: 1538 677 1148 245 2120
Fragments arranged by size:
2120 1538 1148 677 451 247 245 219
178 169 149 117 21
NaeI GCC'GGC
Cuts at: 2762 2762
Size: 7279
NcoI C'CATG_G
Cuts at: 513 1065 2004 2752 513
Size: 552 939 748 5040
Fragments arranged by size:
5040 939 748 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7279
NgoMIV G'CCGG_C
Cuts at: 2760 2760
Size: 7279
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7279
NotI GC'GGCC_GC
Cuts at: 4726 4726
Size: 7279
NsiI A_TGCA'T
Cuts at: 2123 2873 2123
Size: 750 6529
NspI r_CATG'y
Cuts at: 1132 1229 4111 4637 6182 1132
Size: 97 2882 526 1545 2229
Fragments arranged by size:
2882 2229 1545 526 97
PasI CC'CwG_GG
Cuts at: 1348 2013 4274 1348
Size: 665 2261 4353
Fragments arranged by size:
4353 2261 665
PciI A'CATG_T
Cuts at: 6178 6178
Size: 7279
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3043 4318 1108
Size: 1935 1275 4069
Fragments arranged by size:
4069 1935 1275
PmeI GTTT'AAAC
Cuts at: 4583 4583
Size: 7279
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5348 5380 6861 6893 5348
Size: 32 1481 32 5734
Fragments arranged by size:
5734 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2837 2837
Size: 7279
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7279
PsiI TTA'TAA
Cuts at: 4849 4849
Size: 7279
PspOMI G'GGCC_C
Cuts at: 2009 2009
Size: 7279
PstI C_TGCA'G
Cuts at: 830 2071 3098 3362 4159 4631 830
Size: 1241 1027 264 797 472 3478
Fragments arranged by size:
3478 1241 1027 797 472 264
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7279
PvuII CAG'CTG
Cuts at: 2226 2494 2643 3040 3157 2226
Size: 268 149 397 117 6348
Fragments arranged by size:
6348 397 268 149 117
SacII CC_GC'GG
Cuts at: 2058 2058
Size: 7279
SalI G'TCGA_C
Cuts at: 1936 4621 1936
Size: 2685 4594
SanDI GG'GwC_CC
Cuts at: 2837 2837
Size: 7279
SbfI CC_TGCA'GG
Cuts at: 4631 4631
Size: 7279
ScaI AGT'ACT
Cuts at: 5527 7239 5527
Size: 1712 5567
SfcI C'TryA_G
Cuts at: 826 1045 2067 2554 3094 3358 4155 4537 4627
Size: 219 1022 487 540 264 797 382 90
Cuts at: 4627 5762 6443 6634 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1135 1022 797 681 540 487 382
264 219 191 90
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7279
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7279
SmaI CCC'GGG
Cuts at: 1872 4606 1872
Size: 2734 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2999 3760 4448 5383 6284 6546
Size: 197 926 1056 761 688 935 901 262
Cuts at: 6546 820
Size: 1553
Fragments arranged by size:
1553 1056 935 926 901 761 688 262
197
SnaBI TAC'GTA
Cuts at: 493 5070 493
Size: 4577 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7279
SphI G_CATG'C
Cuts at: 1132 4111 4637 1132
Size: 2979 526 3774
Fragments arranged by size:
3774 2979 526
SspI AAT'ATT
Cuts at: 5 52 5203 5
Size: 47 5151 2081
Fragments arranged by size:
5151 2081 47
StuI AGG'CCT
Cuts at: 1517 2426 3176 1517
Size: 909 750 5620
Fragments arranged by size:
5620 909 750
StyI C'CwwG_G
Cuts at: 513 1065 2004 2575 2752 3513 3560 3834 4718
Size: 552 939 571 177 761 47 274 884
Cuts at: 4718 513
Size: 3074
Fragments arranged by size:
3074 939 884 761 571 552 274 177
47
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3380 3491 3995 4181 5306 5323 5476
Size: 1255 1800 111 504 186 1125 17 153
Cuts at: 5476 5661 6106 325
Size: 185 445 1498
Fragments arranged by size:
1800 1498 1255 1125 504 445 186 185
153 111 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3258 3597 5525
Size: 274 80 33 51 1443 1281 339 1928
Cuts at: 5525 7237 96
Size: 1712 138
Fragments arranged by size:
1928 1712 1443 1281 339 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2434 2466 3815 3847 7239 7271 2434
Size: 32 1349 32 3392 32 2442
Fragments arranged by size:
3392 2442 1349 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2885 3404 3482 4303 1316
Size: 1569 519 78 821 4292
Fragments arranged by size:
4292 1569 821 519 78
XbaI T'CTAG_A
Cuts at: 4615 4615
Size: 7279
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2618 2735 2956 2618
Size: 117 221 6941
Fragments arranged by size:
6941 221 117
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7279
XmaI C'CCGG_G
Cuts at: 1870 4604 1870
Size: 2734 4545
XmnI GAAnn'nnTTC
Cuts at: 3681 5408 3681
Size: 1727 5552
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5087 276
Size: 53 83 186 936 3553 2468
Fragments arranged by size:
3553 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NsiI NspI PasI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII
SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbeI BsiWI BstZ17I DraIII
EcoICRI FseI FspAI KasI NarI NruI PacI PfoI
PmlI PspXI PsrI RsrII SacI SapI SexAI SfiI
SfoI SrfI SwaI