Restriction map for
pFN21AB4384
(Circular) (Six-base) MAPSORT of: pFN21AB4384.seq Check: 2567 from: 1 to: 7315
pFN21AB4384 7315 bp
With 182 enzymes: SgfI *
January 16, 2009 15:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5125 278
Size: 53 83 186 936 3589 2468
Fragments arranged by size:
3589 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2607 4658 1937
Size: 670 2051 4594
Fragments arranged by size:
4594 2051 670
Acc65I G'GTAC_C
Cuts at: 4636 4636
Size: 7315
AclI AA'CG_TT
Cuts at: 1563 5442 5815 1563
Size: 3879 373 3063
Fragments arranged by size:
3879 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3480 3512 3679 4142 4148 5378 6762 7030
Size: 1848 32 167 463 6 1230 1384 268
Cuts at: 7030 1632
Size: 1917
Fragments arranged by size:
1917 1848 1384 1230 463 268 167 32
6
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7118
AflIII A'CryG_T
Cuts at: 1203 2487 2952 6214 7191 1203
Size: 1284 465 3262 977 1327
Fragments arranged by size:
3262 1327 1284 977 465
AgeI A'CCGG_T
Cuts at: 6146 6146
Size: 7315
AhdI GACnn_n'nnGTC
Cuts at: 2877 6044 2877
Size: 3167 4148
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7315
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7315
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2747 2779 3659 3691 2747
Size: 32 880 32 6371
Fragments arranged by size:
6371 880 32 32
AlwNI CAG_nnn'CTG
Cuts at: 3438 3724 3778 4168 5058 6630 3438
Size: 286 54 390 890 1572 4123
Fragments arranged by size:
4123 1572 890 390 286 54
ApaI G_GGCC'C
Cuts at: 3951 4011 4491 3951
Size: 60 480 6775
Fragments arranged by size:
6775 480 60
ApaLI G'TGCA_C
Cuts at: 2524 2893 5372 6528 2524
Size: 369 2479 1156 3311
Fragments arranged by size:
3311 2479 1156 369
ApoI r'AATT_y
Cuts at: 1493 2840 4624 4850 5002 7111 1493
Size: 1347 1784 226 152 2109 1697
Fragments arranged by size:
2109 1784 1697 1347 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5869 160
Size: 872 4837 1606
Fragments arranged by size:
4837 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7315
AvaI C'yCGr_G
Cuts at: 1870 1943 2061 3194 3304 3397 3644 3800 4071
Size: 73 118 1133 110 93 247 156 271
Cuts at: 4071 4376 4484 4640 1870
Size: 305 108 156 4545
Fragments arranged by size:
4545 1133 305 271 247 156 156 118
110 108 93 73
BamHI G'GATC_C
Cuts at: 4645 5012 4645
Size: 367 6948
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2076 3592 4636 6091 618
Size: 325 217 627 289 1516 1044 1455 1842
Fragments arranged by size:
1842 1516 1455 1044 627 325 289 217
BanII G_rGCy'C
Cuts at: 1391 2150 2655 3195 3801 3951 4011 4072 4491
Size: 759 505 540 606 150 60 61 419
Cuts at: 4491 4600 4634 1391
Size: 109 34 4072
Fragments arranged by size:
4072 759 606 540 505 419 150 109
61 60 34
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3468 3624 3797 928
Size: 971 1569 156 173 4446
Fragments arranged by size:
4446 1569 971 173 156
BbvCI CC'TCA_GC
Cuts at: 3277 3277
Size: 7315
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5506 5540 1903
Size: 34 3569 34 3678
Fragments arranged by size:
3678 3569 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5207 6417 5207
Size: 1210 6105
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7315
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3212 4006 5926
Size: 107 122 71 631 605 1540 794 1920
Cuts at: 5926 136
Size: 1525
Fragments arranged by size:
1920 1540 1525 794 631 605 122 107
71
BglII A'GATC_T
Cuts at: 7310 7310
Size: 7315
BlpI GC'TnA_GC
Cuts at: 2830 3629 4732 2830
Size: 799 1103 5413
Fragments arranged by size:
5413 1103 799
Bme1580I G_kGCm'C
Cuts at: 1790 2528 2897 3384 3951 4011 4491 4551 5376
Size: 738 369 487 567 60 480 60 825
Cuts at: 5376 6532 1790
Size: 1156 2573
Fragments arranged by size:
2573 1156 825 738 567 487 480 369
60 60
BmgBI CAC'GTC
Cuts at: 1318 3058 4084 1318
Size: 1740 1026 4549
Fragments arranged by size:
4549 1740 1026
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3001 3408 3910
Size: 416 53 83 399 24 1584 407 502
Cuts at: 3910 4417 5046 6004 442
Size: 507 629 958 1753
Fragments arranged by size:
1753 1584 958 629 507 502 416 407
399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7315
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3292 4604 3292
Size: 1312 6003
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2981 3800 4160 5975 1239
Size: 1742 819 360 1815 2579
Fragments arranged by size:
2579 1815 1742 819 360
Bpu10I CC'TnA_GC
Cuts at: 2738 3277 4169 2738
Size: 539 892 5884
Fragments arranged by size:
5884 892 539
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2543 5440 6305 6603 2543
Size: 2897 865 298 3255
Fragments arranged by size:
3255 2897 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2957 5978 882
Size: 617 1458 3021 2219
Fragments arranged by size:
3021 2219 1458 617
BsaAI yAC'GTr
Cuts at: 493 1204 2953 5106 493
Size: 711 1749 2153 2702
Fragments arranged by size:
2702 2153 1749 711
BsaBI GATnn'nnATC
Cuts at: 7309 7309
Size: 7315
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3231 5122 5504 6139
Size: 53 83 186 936 1698 1891 382 635
Cuts at: 6139 275
Size: 1451
Fragments arranged by size:
1891 1698 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2925 5748 6146 6420 6567 6978 2925
Size: 2823 398 274 147 411 3262
Fragments arranged by size:
3262 2823 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2150 2180 2193 2223 1178
Size: 30 165 30 747 30 13 30 6270
Fragments arranged by size:
6270 747 165 30 30 30 30 13
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2059 2201 2206 2441 2613 2828 3203 3212
Size: 873 142 5 235 172 215 375 9
Cuts at: 3212 3284 3346 3662 4580 1186
Size: 72 62 316 918 3921
Fragments arranged by size:
3921 918 873 375 316 235 215 172
142 72 62 9 5
BseYI C'CCAG_C
Cuts at: 3207 3356 3684 4555 6518 7014 3207
Size: 149 328 871 1963 496 3508
Fragments arranged by size:
3508 1963 871 496 328 149
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2572 1163
Size: 573 836 5906
Fragments arranged by size:
5906 836 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4765 5526 6554 1540
Size: 275 186 2764 761 1028 2301
Fragments arranged by size:
2764 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2150 2528 2897 3807 5376 5461 6532 2150
Size: 378 369 910 1569 85 1071 2933
Fragments arranged by size:
2933 1569 1071 910 378 369 85
BsmI GAATG_Cn'
Cuts at: 4826 4919 4826
Size: 93 7222
BsmBI CGTCTCn'nnnn_
Cuts at: 2087 3222 2087
Size: 1135 6180
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2150 2528 2655 2897 3195 3384 3801
Size: 399 360 378 127 242 298 189 417
Cuts at: 3801 3807 3951 4011 4072 4491 4551 4600 4634
Size: 6 144 60 61 419 60 49 34
Cuts at: 4634 5376 5461 6532 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 419 417 399 378 360
298 242 189 144 127 85 61 60
60 49 34 6
BspEI T'CCGG_A
Cuts at: 6978 6978
Size: 7315
BspHI T'CATG_A
Cuts at: 2511 5204 6934 2511
Size: 2693 1730 2892
Fragments arranged by size:
2892 2693 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3006 4386 4515 4670 844
Size: 2162 1380 129 155 3489
Fragments arranged by size:
3489 2162 1380 155 129
BsrBI CCG'CTC
Cuts at: 1394 1823 2500 2829 3652 4173 5202 1394
Size: 429 677 329 823 521 1029 3507
Fragments arranged by size:
3507 1029 823 677 521 429 329
BsrDI GCAATG_nn'
Cuts at: 65 1248 2298 5810 5984 65
Size: 1183 1050 3512 174 1396
Fragments arranged by size:
3512 1396 1183 1050 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2079 3807 4476 5959 6146 1700
Size: 110 269 1728 669 1483 187 2869
Fragments arranged by size:
2869 1728 1483 669 269 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7315
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7315
BssSI C'ACGA_G
Cuts at: 5375 6387 5375
Size: 1012 6303
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3438 794
Size: 2644 4671
BstBI TT'CG_AA
Cuts at: 6119 6119
Size: 7315
BstEII G'GTnAC_C
Cuts at: 1184 2308 2530 2801 1184
Size: 1124 222 271 5698
Fragments arranged by size:
5698 1124 271 222
BstYI r'GATC_y
Cuts at: 1972 3905 3928 4645 5012 5397 5414 6866 6952
Size: 1933 23 717 367 385 17 1452 86
Cuts at: 6952 6964 7310 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1933 1452 717 385 367 346 86
23 17 12
Bsu36I CC'TnA_GG
Cuts at: 2811 4232 2811
Size: 1421 5894
BtgI C'CryG_G
Cuts at: 513 1065 2304 3069 3314 3321 3522 3606 3894
Size: 552 1239 765 245 7 201 84 288
Cuts at: 3894 4098 513
Size: 204 3730
Fragments arranged by size:
3730 1239 765 552 288 245 204 201
84 7
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6576
Fragments arranged by size:
6576 683 56
BtsI GCAGTG_nn'
Cuts at: 2584 3139 3688 4831 5625 5645 2584
Size: 555 549 1143 794 20 4254
Fragments arranged by size:
4254 1143 794 555 549 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7315
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7280
DraI TTT'AAA
Cuts at: 4619 4619
Size: 7315
DraIII CAC_nnn'GTG
Cuts at: 2143 3957 4190 4459 2143
Size: 1814 233 269 4999
Fragments arranged by size:
4999 1814 269 233
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6322 809
Size: 507 5006 1802
Fragments arranged by size:
5006 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2824 2917 3108
Size: 54 1425 50 275 18 994 93 191
Cuts at: 3108 3868 4095 4762 5651 7280 8
Size: 760 227 667 889 1629 43
Fragments arranged by size:
1629 1425 994 889 760 667 275 227
191 93 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4762 1537
Size: 275 2950 4090
Fragments arranged by size:
4090 2950 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3152 3521 3895 4143 5245 1353
Size: 1799 369 374 248 1102 3423
Fragments arranged by size:
3423 1799 1102 374 369 248
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2098 3677 5902 6276 6422 387
Size: 1064 138 509 1579 2225 374 146 1280
Fragments arranged by size:
2225 1579 1280 1064 509 374 146 138
EcoICRI GAG'CTC
Cuts at: 2148 2148
Size: 7315
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2981 3480 3512 3679 3800 4142 4148
Size: 393 1349 499 32 167 121 342 6
Cuts at: 4148 4160 5378 5975 6762 7030 1239
Size: 12 1218 597 787 268 1524
Fragments arranged by size:
1524 1349 1218 787 597 499 393 342
268 167 121 32 12 6
EcoNI CCTnn'n_nnAGG
Cuts at: 4604 4604
Size: 7315
EcoO109I rG'GnC_Cy
Cuts at: 4007 4008 4165 4180 4318 4488 4585 4007
Size: 1 157 15 138 170 97 6737
Fragments arranged by size:
6737 170 157 138 97 15 1
EcoRI G'AATT_C
Cuts at: 2840 4624 5002 2840
Size: 1784 378 5153
Fragments arranged by size:
5153 1784 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3181 3181
Size: 7315
FspI TGC'GCA
Cuts at: 5821 5821
Size: 7315
HaeII r_GCGC'y
Cuts at: 1678 4001 6462 1678
Size: 2323 2461 2531
Fragments arranged by size:
2531 2461 2323
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2137 2150 2169 2182 2201
Size: 32 135 32 565 13 19 13 19
Cuts at: 2201 2379 2411 2863 2895 3219 3251 5956 5988
Size: 178 32 452 32 324 32 2705 32
Cuts at: 5988 6030 6062 1373
Size: 42 32 2626
Fragments arranged by size:
2705 2626 565 452 324 178 135 42
32 32 32 32 32 32 32 19
19 13 13
HincII GTy'rAC
Cuts at: 669 1657 1938 2554 3025 4659 4905 669
Size: 988 281 616 471 1634 246 3079
Fragments arranged by size:
3079 1634 988 616 471 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7315
HpaI GTT'AAC
Cuts at: 4905 4905
Size: 7315
KpnI G_GTAC'C
Cuts at: 4640 4640
Size: 7315
MfeI C'AATT_G
Cuts at: 4914 4914
Size: 7315
MluI A'CGCG_T
Cuts at: 7191 7191
Size: 7315
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4137 6429 6613 1305
Size: 2832 2292 184 2007
Fragments arranged by size:
2832 2292 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2919 3110 3870 10
Size: 54 1425 343 1087 191 760 3455
Fragments arranged by size:
3455 1425 1087 760 343 191 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2303 2516 2675 2756 5273 5632 5791
Size: 648 1137 213 159 81 2517 359 159
Cuts at: 5791 518
Size: 2042
Fragments arranged by size:
2517 2042 1137 648 359 213 159 159
81
MspA1I CmG'CkG
Cuts at: 1606 2012 3211 3392 3438 3778 4138 4348 4731
Size: 406 1199 181 46 340 360 210 383
Cuts at: 4731 5408 6556 6801 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1199 1148 677 406 383 360 340
245 210 181 46
NaeI GCC'GGC
Cuts at: 2081 4478 2081
Size: 2397 4918
NcoI C'CATG_G
Cuts at: 513 1065 3069 3314 3522 3606 3894 4098 513
Size: 552 2004 245 208 84 288 204 3730
Fragments arranged by size:
3730 2004 552 288 245 208 204 84
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7315
NgoMIV G'CCGG_C
Cuts at: 2079 4476 2079
Size: 2397 4918
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7315
NotI GC'GGCC_GC
Cuts at: 4762 4762
Size: 7315
NspI r_CATG'y
Cuts at: 1132 1229 2491 4673 6218 1132
Size: 97 1262 2182 1545 2229
Fragments arranged by size:
2229 2182 1545 1262 97
PasI CC'CwG_GG
Cuts at: 1348 2968 1348
Size: 1620 5695
PciI A'CATG_T
Cuts at: 2487 6214 2487
Size: 3727 3588
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2263 2752 3106 3724 3957 4190 1108
Size: 1155 489 354 618 233 233 4233
Fragments arranged by size:
4233 1155 618 489 354 233 233
PfoI T'CCnGG_A
Cuts at: 3756 4161 3756
Size: 405 6910
PmeI GTTT'AAAC
Cuts at: 4619 4619
Size: 7315
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5384 5416 6897 6929 5384
Size: 32 1481 32 5770
Fragments arranged by size:
5770 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 4165 4180 4585 4165
Size: 15 405 6895
Fragments arranged by size:
6895 405 15
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7315
PsiI TTA'TAA
Cuts at: 4885 4885
Size: 7315
PspOMI G'GGCC_C
Cuts at: 3947 4007 4487 3947
Size: 60 480 6775
Fragments arranged by size:
6775 480 60
PspXI vC'TCGA_Gb
Cuts at: 4071 4071
Size: 7315
PstI C_TGCA'G
Cuts at: 830 3003 4667 830
Size: 2173 1664 3478
Fragments arranged by size:
3478 2173 1664
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7315
PvuII CAG'CTG
Cuts at: 2012 3211 3392 3438 3778 4138 2012
Size: 1199 181 46 340 360 5189
Fragments arranged by size:
5189 1199 360 340 181 46
SacI G_AGCT'C
Cuts at: 2150 2150
Size: 7315
SalI G'TCGA_C
Cuts at: 1936 4657 1936
Size: 2721 4594
SanDI GG'GwC_CC
Cuts at: 4180 4180
Size: 7315
SapI GCTCTTCn'nnn_
Cuts at: 3521 4143 3521
Size: 622 6693
SbfI CC_TGCA'GG
Cuts at: 4667 4667
Size: 7315
ScaI AGT'ACT
Cuts at: 5563 7275 5563
Size: 1712 5603
SexAI A'CCwGG_T
Cuts at: 4186 4186
Size: 7315
SfcI C'TryA_G
Cuts at: 826 1045 2999 3732 4435 4663 5798 6479 6670
Size: 219 1954 733 703 228 1135 681 191
Cuts at: 6670 826
Size: 1471
Fragments arranged by size:
1954 1471 1135 733 703 681 228 219
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7315
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7315
SmaI CCC'GGG
Cuts at: 1872 2063 3399 4378 4642 1872
Size: 191 1336 979 264 4545
Fragments arranged by size:
4545 1336 979 264 191
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2558 4071 5419 6320 6582 820
Size: 197 926 615 1513 1348 901 262 1553
Fragments arranged by size:
1553 1513 1348 926 901 615 262 197
SnaBI TAC'GTA
Cuts at: 493 5106 493
Size: 4613 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7315
SphI G_CATG'C
Cuts at: 1132 4673 1132
Size: 3541 3774
SspI AAT'ATT
Cuts at: 5 52 5239 5
Size: 47 5187 2081
Fragments arranged by size:
5187 2081 47
StuI AGG'CCT
Cuts at: 1517 2165 1517
Size: 648 6667
StyI C'CwwG_G
Cuts at: 513 1065 2286 2898 3069 3314 3522 3537 3606
Size: 552 1221 612 171 245 208 15 69
Cuts at: 3606 3894 4098 4754 513
Size: 288 204 656 3074
Fragments arranged by size:
3074 1221 656 612 552 288 245 208
204 171 69 15
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2712 3359 4316 4436 5342 5359 5512
Size: 1255 1132 647 957 120 906 17 153
Cuts at: 5512 5697 6142 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 1132 957 906 647 445 185
153 120 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5561 7273 96
Size: 274 80 33 51 1443 3584 1712 138
Fragments arranged by size:
3584 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2193 2225 3585 3617 7275 7307 2193
Size: 32 1360 32 3658 32 2201
Fragments arranged by size:
3658 2201 1360 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 4048 1316
Size: 2732 4583
XbaI T'CTAG_A
Cuts at: 4651 4651
Size: 7315
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2176 3241 3353 4318 2176
Size: 1065 112 965 5173
Fragments arranged by size:
5173 1065 965 112
XhoI C'TCGA_G
Cuts at: 1943 4071 1943
Size: 2128 5187
XmaI C'CCGG_G
Cuts at: 1870 2061 3397 4376 4640 1870
Size: 191 1336 979 264 4545
Fragments arranged by size:
4545 1336 979 264 191
XmnI GAAnn'nnTTC
Cuts at: 3515 5444 3515
Size: 1929 5386
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5123 276
Size: 53 83 186 936 3589 2468
Fragments arranged by size:
3589 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI
SanDI SapI SbfI ScaI SexAI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BbeI BfrBI BsiWI
BstXI BstZ17I EcoRV FseI FspAI KasI NarI NruI
NsiI PacI PmlI PsrI RsrII SacII SfiI SfoI
SrfI SwaI