Restriction map for
pFN21AB4375
(Circular) (Six-base) MAPSORT of: pFN21AB4375.seq Check: 8402 from: 1 to: 5872
pFN21AB4375 5872 bp
With 182 enzymes: SgfI *
October 20, 2009 10:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2175 2175
Size: 5872
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3682 278
Size: 53 83 186 936 2146 2468
Fragments arranged by size:
2468 2146 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2925 3215 1937
Size: 988 290 4594
Fragments arranged by size:
4594 988 290
Acc65I G'GTAC_C
Cuts at: 3193 3193
Size: 5872
AclI AA'CG_TT
Cuts at: 1563 3999 4372 1563
Size: 2436 373 3063
Fragments arranged by size:
3063 2436 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2466 3935 5319 5587 1632
Size: 834 1469 1384 268 1917
Fragments arranged by size:
1917 1469 1384 834 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5675
AflIII A'CryG_T
Cuts at: 1203 4771 5748 1203
Size: 3568 977 1327
Fragments arranged by size:
3568 1327 977
AgeI A'CCGG_T
Cuts at: 4703 4703
Size: 5872
AhdI GACnn_n'nnGTC
Cuts at: 4601 4601
Size: 5872
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5872
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3067 1184
Size: 1883 3989
AlwNI CAG_nnn'CTG
Cuts at: 3615 5187 3615
Size: 1572 4300
ApaI G_GGCC'C
Cuts at: 2095 2095
Size: 5872
ApaLI G'TGCA_C
Cuts at: 2992 3092 3929 5085 2992
Size: 100 837 1156 3779
Fragments arranged by size:
3779 1156 837 100
ApoI r'AATT_y
Cuts at: 1493 2413 2607 2625 3181 3407 3559 5668 1493
Size: 920 194 18 556 226 152 2109 1697
Fragments arranged by size:
2109 1697 920 556 226 194 152 18
AseI AT'TA_AT
Cuts at: 160 1032 4426 160
Size: 872 3394 1606
Fragments arranged by size:
3394 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5872
AvaI C'yCGr_G
Cuts at: 1870 1943 2100 2245 2733 3197 1870
Size: 73 157 145 488 464 4545
Fragments arranged by size:
4545 488 464 157 145 73
BamHI G'GATC_C
Cuts at: 3202 3569 3202
Size: 367 5505
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2249 2258 2267 2394 2900
Size: 325 217 627 462 9 9 127 506
Cuts at: 2900 3193 4648 618
Size: 293 1455 1842
Fragments arranged by size:
1842 1455 627 506 462 325 293 217
127 9 9
BanII G_rGCy'C
Cuts at: 1391 2095 3191 1391
Size: 704 1096 4072
Fragments arranged by size:
4072 1096 704
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4901
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4063 4097 1903
Size: 34 2126 34 3678
Fragments arranged by size:
3678 2126 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3764 4974 3764
Size: 1210 4662
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5872
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2241 2286 4483
Size: 107 122 71 631 605 569 45 2197
Cuts at: 4483 136
Size: 1525
Fragments arranged by size:
2197 1525 631 605 569 122 107 71
45
BglII A'GATC_T
Cuts at: 5867 5867
Size: 5872
BlpI GC'TnA_GC
Cuts at: 3289 3289
Size: 5872
Bme1580I G_kGCm'C
Cuts at: 1790 2095 2252 2397 2996 3096 3933 5089 1790
Size: 305 157 145 599 100 837 1156 2573
Fragments arranged by size:
2573 1156 837 599 305 157 145 100
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5872
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2504 3603 4561
Size: 416 53 83 399 24 1087 1099 958
Cuts at: 4561 442
Size: 1753
Fragments arranged by size:
1753 1099 1087 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5872
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2215 2215
Size: 5872
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2550 2568 2646 2681 2903 4532 1239
Size: 1311 18 78 35 222 1629 2579
Fragments arranged by size:
2579 1629 1311 222 78 35 18
Bpu10I CC'TnA_GC
Cuts at: 2273 2337 2273
Size: 64 5808
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2664 3044 3997 4862 5160 2664
Size: 380 953 865 298 3376
Fragments arranged by size:
3376 953 865 380 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2832 4535 882
Size: 617 1333 1703 2219
Fragments arranged by size:
2219 1703 1333 617
BsaAI yAC'GTr
Cuts at: 493 1204 3663 493
Size: 711 2459 2702
Fragments arranged by size:
2702 2459 711
BsaBI GATnn'nnATC
Cuts at: 5866 5866
Size: 5872
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3048 3679 4061 4696
Size: 53 83 186 936 1515 631 382 635
Cuts at: 4696 275
Size: 1451
Fragments arranged by size:
1515 1451 936 635 631 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4305 4703 4977 5124 5535 4305
Size: 398 274 147 411 4642
Fragments arranged by size:
4642 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2366 2396 1178
Size: 30 165 30 963 30 4654
Fragments arranged by size:
4654 963 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3047 1186
Size: 1861 4011
BseYI C'CCAG_C
Cuts at: 3151 5075 5571 3151
Size: 1924 496 3452
Fragments arranged by size:
3452 1924 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5299
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3322 4083 5111 1540
Size: 275 186 1321 761 1028 2301
Fragments arranged by size:
2301 1321 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2817 2996 3096 3933 4018 5089 2817
Size: 179 100 837 85 1071 3600
Fragments arranged by size:
3600 1071 837 179 100 85
BsmI GAATG_Cn'
Cuts at: 3383 3476 3383
Size: 93 5779
BsmBI CGTCTCn'nnnn_
Cuts at: 3002 3002
Size: 5872
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2095 2252 2397 2817 2996 3096 3191
Size: 399 305 157 145 420 179 100 95
Cuts at: 3191 3933 4018 5089 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 420 399 305 179 157
145 100 95 85
BspEI T'CCGG_A
Cuts at: 5535 5535
Size: 5872
BspHI T'CATG_A
Cuts at: 3761 5491 3761
Size: 1730 4142
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2175 3227 844
Size: 1331 1052 3489
Fragments arranged by size:
3489 1331 1052
BsrBI CCG'CTC
Cuts at: 1394 1823 3759 1394
Size: 429 1936 3507
Fragments arranged by size:
3507 1936 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2497 4367 4541 65
Size: 1183 1249 1870 174 1396
Fragments arranged by size:
1870 1396 1249 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4516 4703 1700
Size: 110 2706 187 2869
Fragments arranged by size:
2869 2706 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5872
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5872
BssSI C'ACGA_G
Cuts at: 3932 4944 3932
Size: 1012 4860
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5872
BstBI TT'CG_AA
Cuts at: 4676 4676
Size: 5872
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5872
BstXI CCAn_nnnn'nTGG
Cuts at: 3151 3151
Size: 5872
BstYI r'GATC_y
Cuts at: 1972 3202 3569 3954 3971 5423 5509 5521 5867
Size: 1230 367 385 17 1452 86 12 346
Cuts at: 5867 1972
Size: 1977
Fragments arranged by size:
1977 1452 1230 385 367 346 86 17
12
BstZ17I GTA'TAC
Cuts at: 2926 2926
Size: 5872
BtgI C'CryG_G
Cuts at: 513 1065 2004 2054 513
Size: 552 939 50 4331
Fragments arranged by size:
4331 939 552 50
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5133
Fragments arranged by size:
5133 683 56
BtsI GCAGTG_nn'
Cuts at: 2996 3388 4182 4202 2996
Size: 392 794 20 4666
Fragments arranged by size:
4666 794 392 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5872
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3118 3153 529
Size: 35 2554 35 3248
Fragments arranged by size:
3248 2554 35 35
DraI TTT'AAA
Cuts at: 3176 3176
Size: 5872
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2655 4879 809
Size: 507 1339 2224 1802
Fragments arranged by size:
2224 1802 1339 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3319 4208 5837
Size: 54 1425 50 275 18 1489 889 1629
Cuts at: 5837 8
Size: 43
Fragments arranged by size:
1629 1489 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3319 1537
Size: 275 1507 4090
Fragments arranged by size:
4090 1507 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2135 3802 1353
Size: 782 1667 3423
Fragments arranged by size:
3423 1667 782
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4459 4833 4979 387
Size: 1064 138 2870 374 146 1280
Fragments arranged by size:
2870 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2466 2550 2568 2646 2681 2903 3935
Size: 393 834 84 18 78 35 222 1032
Cuts at: 3935 4532 5319 5587 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1032 834 787 597 393 268 222
84 78 35 18
EcoNI CCTnn'n_nnAGG
Cuts at: 3059 3059
Size: 5872
EcoO109I rG'GnC_Cy
Cuts at: 2091 2092 2543 2570 2579 2588 2597 2666 2684
Size: 1 451 27 9 9 9 69 18
Cuts at: 2684 2702 2091
Size: 18 5261
Fragments arranged by size:
5261 451 69 27 18 18 9 9
9 1
EcoRI G'AATT_C
Cuts at: 2607 2625 3181 3559 2607
Size: 18 556 378 4920
Fragments arranged by size:
4920 556 378 18
FspI TGC'GCA
Cuts at: 4378 4378
Size: 5872
HaeII r_GCGC'y
Cuts at: 1678 2054 5019 1678
Size: 376 2965 2531
Fragments arranged by size:
2965 2531 376
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4513 4545 4587 4619 1373
Size: 32 135 32 2941 32 42 32 2626
Fragments arranged by size:
2941 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3216 3462 669
Size: 988 281 1278 246 3079
Fragments arranged by size:
3079 1278 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5872
HpaI GTT'AAC
Cuts at: 3462 3462
Size: 5872
KpnI G_GTAC'C
Cuts at: 3197 3197
Size: 5872
MfeI C'AATT_G
Cuts at: 3471 3471
Size: 5872
MluI A'CGCG_T
Cuts at: 5748 5748
Size: 5872
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4986 5170 1305
Size: 3681 184 2007
Fragments arranged by size:
3681 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4050
Fragments arranged by size:
4050 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2837 3113 3830 4189 4348 518
Size: 648 1671 276 717 359 159 2042
Fragments arranged by size:
2042 1671 717 648 359 276 159
MspA1I CmG'CkG
Cuts at: 1606 2056 2121 2750 2874 3288 3965 5113 5358
Size: 450 65 629 124 414 677 1148 245
Cuts at: 5358 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 629 450 414 245 124
65
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4381
Fragments arranged by size:
4381 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5872
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5872
NotI GC'GGCC_GC
Cuts at: 3319 3319
Size: 5872
NspI r_CATG'y
Cuts at: 1132 1229 3230 4775 1132
Size: 97 2001 1545 2229
Fragments arranged by size:
2229 2001 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2593 1348
Size: 1245 4627
PciI A'CATG_T
Cuts at: 4771 4771
Size: 5872
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5872
PfoI T'CCnGG_A
Cuts at: 2548 2566 2584 2620 2629 2548
Size: 18 18 36 9 5791
Fragments arranged by size:
5791 36 18 18 9
PmeI GTTT'AAAC
Cuts at: 3176 3176
Size: 5872
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3941 3973 5454 5486 3941
Size: 32 1481 32 4327
Fragments arranged by size:
4327 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2543 2570 2579 2588 2597 2666 2684 2702 2543
Size: 27 9 9 9 69 18 18 5713
Fragments arranged by size:
5713 69 27 18 18 9 9 9
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5872
PsiI TTA'TAA
Cuts at: 3442 3442
Size: 5872
PspOMI G'GGCC_C
Cuts at: 2091 2091
Size: 5872
PstI C_TGCA'G
Cuts at: 830 2047 2991 3224 830
Size: 1217 944 233 3478
Fragments arranged by size:
3478 1217 944 233
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5872
PvuII CAG'CTG
Cuts at: 2121 2750 2121
Size: 629 5243
SacII CC_GC'GG
Cuts at: 2057 2057
Size: 5872
SalI G'TCGA_C
Cuts at: 1936 3214 1936
Size: 1278 4594
SanDI GG'GwC_CC
Cuts at: 2597 2597
Size: 5872
SapI GCTCTTCn'nnn_
Cuts at: 2135 2135
Size: 5872
SbfI CC_TGCA'GG
Cuts at: 3224 3224
Size: 5872
ScaI AGT'ACT
Cuts at: 4120 5832 4120
Size: 1712 4160
SexAI A'CCwGG_T
Cuts at: 2653 2653
Size: 5872
SfcI C'TryA_G
Cuts at: 826 1045 2043 2762 2914 2987 3220 4355 5036
Size: 219 998 719 152 73 233 1135 681
Cuts at: 5036 5227 826
Size: 191 1471
Fragments arranged by size:
1471 1135 998 719 681 233 219 191
152 73
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5872
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5872
SmaI CCC'GGG
Cuts at: 1872 2102 3199 1872
Size: 230 1097 4545
Fragments arranged by size:
4545 1097 230
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2679 2733 3059 3976 4877 5139
Size: 197 926 736 54 326 917 901 262
Cuts at: 5139 820
Size: 1553
Fragments arranged by size:
1553 926 917 901 736 326 262 197
54
SnaBI TAC'GTA
Cuts at: 493 3663 493
Size: 3170 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5872
SphI G_CATG'C
Cuts at: 1132 3230 1132
Size: 2098 3774
SrfI GCCC'GGGC
Cuts at: 2102 2102
Size: 5872
SspI AAT'ATT
Cuts at: 5 52 3796 5
Size: 47 3744 2081
Fragments arranged by size:
3744 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5872
StyI C'CwwG_G
Cuts at: 513 1065 2004 2236 2320 2770 2847 3311 513
Size: 552 939 232 84 450 77 464 3074
Fragments arranged by size:
3074 939 552 464 450 232 84 77
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3143 3899 3916 4069 4254 4699 325
Size: 1255 1563 756 17 153 185 445 1498
Fragments arranged by size:
1563 1498 1255 756 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4118 5830 96
Size: 274 80 33 51 1443 2141 1712 138
Fragments arranged by size:
2141 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2366 2398 5832 5864 2366
Size: 32 3434 32 2374
Fragments arranged by size:
3434 2374 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5872
XbaI T'CTAG_A
Cuts at: 3208 3208
Size: 5872
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2705 2959 2705
Size: 254 5618
XhoI C'TCGA_G
Cuts at: 1943 2733 1943
Size: 790 5082
XmaI C'CCGG_G
Cuts at: 1870 2100 3197 1870
Size: 230 1097 4545
Fragments arranged by size:
4545 1097 230
XmnI GAAnn'nnTTC
Cuts at: 2417 2981 4001 2417
Size: 564 1020 4288
Fragments arranged by size:
4288 1020 564
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3680 276
Size: 53 83 186 936 2146 2468
Fragments arranged by size:
2468 2146 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SacII SalI SanDI SapI SbfI ScaI SexAI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BsiWI Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI
PspXI PsrI RsrII SacI SfiI SfoI SwaI